UniProtKB - P0AFK0 (PMBA_ECOLI)
Protein
Metalloprotease PmbA
Gene
pmbA
Organism
Escherichia coli (strain K12)
Status
Functioni
Metalloprotease involved in CcdA degradation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA).1 Publication
Miscellaneous
In strains containing the pMccB17 plasmid, required for the maturation and secretion of the antibiotic bacteriocin microcin B17 (MccB17).1 Publication
GO - Molecular functioni
- metallopeptidase activity Source: UniProtKB-KW
GO - Biological processi
- proteolysis Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10741-MONOMER MetaCyc:EG10741-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:pmbA Synonyms:tldE Ordered Locus Names:b4235, JW4194 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000142351 | 1 – 450 | Metalloprotease PmbAAdd BLAST | 450 |
Proteomic databases
jPOSTi | P0AFK0 |
PaxDbi | P0AFK0 |
PRIDEi | P0AFK0 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262248, 15 interactors |
DIPi | DIP-47844N |
IntActi | P0AFK0, 6 interactors |
STRINGi | 511145.b4235 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFK0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase U62 family.Curated
Phylogenomic databases
eggNOGi | COG0312, Bacteria |
HOGENOMi | CLU_026425_0_0_6 |
InParanoidi | P0AFK0 |
PhylomeDBi | P0AFK0 |
Family and domain databases
Gene3Di | 3.30.2290.10, 1 hit |
InterProi | View protein in InterPro IPR002510, TldD/PmbA IPR035068, TldD/PmbA_N IPR036059, TldD/PmbA_sf |
Pfami | View protein in Pfam PF01523, PmbA_TldD, 1 hit |
SUPFAMi | SSF111283, SSF111283, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFK0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALAMKVISQ VEAQRKILEE AVSTALELAS GKSDGAEVAV SKTTGISVST
60 70 80 90 100
RYGEVENVEF NSDGALGITV YHQNRKGSAS STDLSPQAIA RTVQAALDIA
110 120 130 140 150
RYTSPDPCAG VADKELLAFD APDLDLFHPA EVSPDEAIEL AARAEQAALQ
160 170 180 190 200
ADKRITNTEG GSFNSHYGVK VFGNSHGMLQ GYCSTRHSLS SCVIAEENGD
210 220 230 240 250
MERDYAYTIG RAMSDLQTPE WVGADCARRT LSRLSPRKLS TMKAPVIFAN
260 270 280 290 300
EVATGLFGHL VGAIAGGSVY RKSTFLLDSL GKQILPDWLT IEEHPHLLKG
310 320 330 340 350
LASTPFDSEG VRTERRDIIK DGILTQWLLT SYSARKLGLK STGHAGGIHN
360 370 380 390 400
WRIAGQGLSF EQMLKEMGTG LVVTELMGQG VSAITGDYSR GAAGFWVENG
410 420 430 440 450
EIQYPVSEIT IAGNLKDMWR NIVTVGNDIE TRSNIQCGSV LLPEMKIAGQ
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54152 Genomic DNA Translation: CAA38091.1 D44452 Genomic DNA Translation: BAA07915.1 U14003 Genomic DNA Translation: AAA97132.1 U00096 Genomic DNA Translation: AAC77192.1 AP009048 Genomic DNA Translation: BAE78235.1 |
PIRi | S13730 |
RefSeqi | NP_418656.1, NC_000913.3 WP_001162171.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC77192; AAC77192; b4235 BAE78235; BAE78235; BAE78235 |
GeneIDi | 948750 |
KEGGi | ecj:JW4194 eco:b4235 |
PATRICi | fig|1411691.4.peg.2467 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X54152 Genomic DNA Translation: CAA38091.1 D44452 Genomic DNA Translation: BAA07915.1 U14003 Genomic DNA Translation: AAA97132.1 U00096 Genomic DNA Translation: AAC77192.1 AP009048 Genomic DNA Translation: BAE78235.1 |
PIRi | S13730 |
RefSeqi | NP_418656.1, NC_000913.3 WP_001162171.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5NJ5 | X-ray | 1.90 | B/D | 1-450 | [»] | |
5NJ9 | X-ray | 1.25 | B/D | 1-450 | [»] | |
5NJA | X-ray | 1.40 | B/D | 1-450 | [»] | |
5NJB | X-ray | 1.50 | B/D | 1-450 | [»] | |
5NJC | X-ray | 1.35 | B/D | 1-450 | [»] | |
5NJF | X-ray | 1.42 | B/D | 1-450 | [»] | |
SMRi | P0AFK0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262248, 15 interactors |
DIPi | DIP-47844N |
IntActi | P0AFK0, 6 interactors |
STRINGi | 511145.b4235 |
Proteomic databases
jPOSTi | P0AFK0 |
PaxDbi | P0AFK0 |
PRIDEi | P0AFK0 |
Genome annotation databases
EnsemblBacteriai | AAC77192; AAC77192; b4235 BAE78235; BAE78235; BAE78235 |
GeneIDi | 948750 |
KEGGi | ecj:JW4194 eco:b4235 |
PATRICi | fig|1411691.4.peg.2467 |
Organism-specific databases
EchoBASEi | EB0734 |
Phylogenomic databases
eggNOGi | COG0312, Bacteria |
HOGENOMi | CLU_026425_0_0_6 |
InParanoidi | P0AFK0 |
PhylomeDBi | P0AFK0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10741-MONOMER MetaCyc:EG10741-MONOMER |
Miscellaneous databases
PROi | PR:P0AFK0 |
Family and domain databases
Gene3Di | 3.30.2290.10, 1 hit |
InterProi | View protein in InterPro IPR002510, TldD/PmbA IPR035068, TldD/PmbA_N IPR036059, TldD/PmbA_sf |
Pfami | View protein in Pfam PF01523, PmbA_TldD, 1 hit |
SUPFAMi | SSF111283, SSF111283, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PMBA_ECOLI | |
Accessioni | P0AFK0Primary (citable) accession number: P0AFK0 Secondary accession number(s): P24231, Q2M671 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families