UniProtKB - P0AFJ5 (PHOB_ECOLI)
Protein
Phosphate regulon transcriptional regulatory protein PhoB
Gene
phoB
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 129 – 227 | OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST | 99 |
GO - Molecular functioni
- bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
- DNA-binding transcription activator activity Source: GO_Central
- identical protein binding Source: IntAct
- phosphorelay response regulator activity Source: GO_Central
- transcription regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- phosphate ion transport Source: UniProtKB-KW
- regulation of DNA-templated transcription, initiation Source: EcoCyc
Keywordsi
Molecular function | Activator, DNA-binding |
Biological process | Phosphate transport, Transcription, Transcription regulation, Transport, Two-component regulatory system |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOB-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Phosphate regulon transcriptional regulatory protein PhoB |
Gene namesi | Name:phoB Ordered Locus Names:b0399, JW0389 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: GO_Central
Other locations
- protein-DNA complex Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081186 | 1 – 229 | Phosphate regulon transcriptional regulatory protein PhoBAdd BLAST | 229 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 53 | 4-aspartylphosphatePROSITE-ProRule annotation1 Publication | 1 |
Post-translational modificationi
Phosphorylated by PhoR or CreC.1 Publication
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AFJ5 |
PaxDbi | P0AFJ5 |
PRIDEi | P0AFJ5 |
Interactioni
Binary interactionsi
P0AFJ5
With | #Exp. | IntAct |
---|---|---|
itself | 9 | EBI-1116564,EBI-1116564 |
GO - Molecular functioni
- bacterial-type RNA polymerase holo enzyme binding Source: EcoCyc
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4259816, 27 interactors 849435, 5 interactors |
DIPi | DIP-35852N |
IntActi | P0AFJ5, 24 interactors |
STRINGi | 511145.b0399 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AFJ5 |
SMRi | P0AFJ5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFJ5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 120 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 117 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_4_6 |
InParanoidi | P0AFJ5 |
PhylomeDBi | P0AFJ5 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR011879, Sig_transdc_resp-reg_PhoB IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
TIGRFAMsi | TIGR02154, PhoB, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFJ5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI
60 70 80 90 100
LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD
110 120 130 140 150
DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE
160 170 180 190 200
EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR
210 220
RLRKALEPGG HDRMVQTVRG TGYRFSTRF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04026 Genomic DNA Translation: CAA27659.1 U73857 Genomic DNA Translation: AAB18123.1 U00096 Genomic DNA Translation: AAC73502.1 AP009048 Genomic DNA Translation: BAE76179.1 |
PIRi | A24256, RGECFB |
RefSeqi | NP_414933.1, NC_000913.3 WP_000113933.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73502; AAC73502; b0399 BAE76179; BAE76179; BAE76179 |
GeneIDi | 57731575 945046 |
KEGGi | ecj:JW0389 eco:b0399 |
PATRICi | fig|1411691.4.peg.1879 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X04026 Genomic DNA Translation: CAA27659.1 U73857 Genomic DNA Translation: AAB18123.1 U00096 Genomic DNA Translation: AAC73502.1 AP009048 Genomic DNA Translation: BAE76179.1 |
PIRi | A24256, RGECFB |
RefSeqi | NP_414933.1, NC_000913.3 WP_000113933.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B00 | X-ray | 1.88 | A/B | 1-127 | [»] | |
1GXP | X-ray | 2.50 | A/B/E/F | 124-229 | [»] | |
1GXQ | X-ray | 2.00 | A | 124-229 | [»] | |
1QQI | NMR | - | A | 126-229 | [»] | |
1ZES | X-ray | 1.90 | A/B/C | 1-125 | [»] | |
2IYN | X-ray | 2.08 | A/B/C | 1-127 | [»] | |
2JB9 | X-ray | 1.70 | A/B | 1-127 | [»] | |
2JBA | X-ray | 1.45 | A/B | 1-127 | [»] | |
2Z33 | NMR | - | A | 126-229 | [»] | |
3T72 | X-ray | 4.33 | 1/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l | 128-229 | [»] | |
BMRBi | P0AFJ5 | |||||
SMRi | P0AFJ5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259816, 27 interactors 849435, 5 interactors |
DIPi | DIP-35852N |
IntActi | P0AFJ5, 24 interactors |
STRINGi | 511145.b0399 |
Proteomic databases
jPOSTi | P0AFJ5 |
PaxDbi | P0AFJ5 |
PRIDEi | P0AFJ5 |
Genome annotation databases
EnsemblBacteriai | AAC73502; AAC73502; b0399 BAE76179; BAE76179; BAE76179 |
GeneIDi | 57731575 945046 |
KEGGi | ecj:JW0389 eco:b0399 |
PATRICi | fig|1411691.4.peg.1879 |
Organism-specific databases
EchoBASEi | EB0721 |
Phylogenomic databases
eggNOGi | COG0745, Bacteria |
HOGENOMi | CLU_000445_30_4_6 |
InParanoidi | P0AFJ5 |
PhylomeDBi | P0AFJ5 |
Enzyme and pathway databases
BioCyci | EcoCyc:PHOB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFJ5 |
PROi | PR:P0AFJ5 |
Family and domain databases
CDDi | cd00383, trans_reg_C, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR001867, OmpR/PhoB-type_DNA-bd IPR011879, Sig_transdc_resp-reg_PhoB IPR001789, Sig_transdc_resp-reg_receiver IPR039420, WalR-like IPR036388, WH-like_DNA-bd_sf |
PANTHERi | PTHR48111, PTHR48111, 1 hit |
Pfami | View protein in Pfam PF00072, Response_reg, 1 hit PF00486, Trans_reg_C, 1 hit |
SMARTi | View protein in SMART SM00448, REC, 1 hit SM00862, Trans_reg_C, 1 hit |
SUPFAMi | SSF52172, SSF52172, 1 hit |
TIGRFAMsi | TIGR02154, PhoB, 1 hit |
PROSITEi | View protein in PROSITE PS51755, OMPR_PHOB, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PHOB_ECOLI | |
Accessioni | P0AFJ5Primary (citable) accession number: P0AFJ5 Secondary accession number(s): P08402, Q2MC27 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1988 |
Last sequence update: | August 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references