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Entry version 112 (08 May 2019)
Sequence version 1 (01 Aug 1988)
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Protein

Phosphate regulon transcriptional regulatory protein PhoB

Gene

phoB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB and PhoR when phosphate is limited.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi129 – 227OmpR/PhoB-typePROSITE-ProRule annotationAdd BLAST99

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding
Biological processPhosphate transport, Transcription, Transcription regulation, Transport, Two-component regulatory system

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:PHOB-MONOMER
ECOL316407:JW0389-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Phosphate regulon transcriptional regulatory protein PhoB
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:phoB
Ordered Locus Names:b0399, JW0389
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

Escherichia coli strain K12 genome database

More...
EcoGenei
EG10728 phoB

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000811861 – 229Phosphate regulon transcriptional regulatory protein PhoBAdd BLAST229

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei534-aspartylphosphatePROSITE-ProRule annotation1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by PhoR or CreC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0AFJ5

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0AFJ5

PRoteomics IDEntifications database

More...
PRIDEi
P0AFJ5

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

WithEntry#Exp.IntActNotes
itself9EBI-1116564,EBI-1116564

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
4259816, 27 interactors

Database of interacting proteins

More...
DIPi
DIP-35852N

Protein interaction database and analysis system

More...
IntActi
P0AFJ5, 24 interactors

STRING: functional protein association networks

More...
STRINGi
511145.b0399

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1229
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B00X-ray1.88A/B1-127[»]
1GXPX-ray2.50A/B/E/F124-229[»]
1GXQX-ray2.00A124-229[»]
1QQINMR-A126-229[»]
1ZESX-ray1.90A/B/C1-125[»]
2IYNX-ray2.08A/B/C1-127[»]
2JB9X-ray1.70A/B1-127[»]
2JBAX-ray1.45A/B1-127[»]
2Z33NMR-A126-229[»]
3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P0AFJ5

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...
EvolutionaryTracei
P0AFJ5

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini4 – 120Response regulatoryPROSITE-ProRule annotationAdd BLAST117

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
ENOG4105CK6 Bacteria
COG0745 LUCA

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000034819

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0AFJ5

KEGG Orthology (KO)

More...
KOi
K07657

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0AFJ5

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00156 REC, 1 hit
cd00383 trans_reg_C, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.10.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011006 CheY-like_superfamily
IPR001867 OmpR/PhoB-type_DNA-bd
IPR011879 Sig_transdc_resp-reg_PhoB
IPR001789 Sig_transdc_resp-reg_receiver
IPR039420 WalR-like
IPR036388 WH-like_DNA-bd_sf

The PANTHER Classification System

More...
PANTHERi
PTHR26402 PTHR26402, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00072 Response_reg, 1 hit
PF00486 Trans_reg_C, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00448 REC, 1 hit
SM00862 Trans_reg_C, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF52172 SSF52172, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR02154 PhoB, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS51755 OMPR_PHOB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AFJ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MARRILVVED EAPIREMVCF VLEQNGFQPV EAEDYDSAVN QLNEPWPDLI
60 70 80 90 100
LLDWMLPGGS GIQFIKHLKR ESMTRDIPVV MLTARGEEED RVRGLETGAD
110 120 130 140 150
DYITKPFSPK ELVARIKAVM RRISPMAVEE VIEMQGLSLD PTSHRVMAGE
160 170 180 190 200
EPLEMGPTEF KLLHFFMTHP ERVYSREQLL NHVWGTNVYV EDRTVDVHIR
210 220
RLRKALEPGG HDRMVQTVRG TGYRFSTRF
Length:229
Mass (Da):26,433
Last modified:August 1, 1988 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iEB46BA282F1F2C23
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X04026 Genomic DNA Translation: CAA27659.1
U73857 Genomic DNA Translation: AAB18123.1
U00096 Genomic DNA Translation: AAC73502.1
AP009048 Genomic DNA Translation: BAE76179.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A24256 RGECFB

NCBI Reference Sequences

More...
RefSeqi
NP_414933.1, NC_000913.3
WP_000113933.1, NZ_LN832404.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC73502; AAC73502; b0399
BAE76179; BAE76179; BAE76179

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
945046

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW0389
eco:b0399

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.1879

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04026 Genomic DNA Translation: CAA27659.1
U73857 Genomic DNA Translation: AAB18123.1
U00096 Genomic DNA Translation: AAC73502.1
AP009048 Genomic DNA Translation: BAE76179.1
PIRiA24256 RGECFB
RefSeqiNP_414933.1, NC_000913.3
WP_000113933.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B00X-ray1.88A/B1-127[»]
1GXPX-ray2.50A/B/E/F124-229[»]
1GXQX-ray2.00A124-229[»]
1QQINMR-A126-229[»]
1ZESX-ray1.90A/B/C1-125[»]
2IYNX-ray2.08A/B/C1-127[»]
2JB9X-ray1.70A/B1-127[»]
2JBAX-ray1.45A/B1-127[»]
2Z33NMR-A126-229[»]
3T72X-ray4.331/4/5/8/9/A/B/E/F/I/J/M/N/R/S/V/W/Z/c/d/g/h/k/l128-229[»]
SMRiP0AFJ5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259816, 27 interactors
DIPiDIP-35852N
IntActiP0AFJ5, 24 interactors
STRINGi511145.b0399

Proteomic databases

jPOSTiP0AFJ5
PaxDbiP0AFJ5
PRIDEiP0AFJ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73502; AAC73502; b0399
BAE76179; BAE76179; BAE76179
GeneIDi945046
KEGGiecj:JW0389
eco:b0399
PATRICifig|1411691.4.peg.1879

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB0721
EcoGeneiEG10728 phoB

Phylogenomic databases

eggNOGiENOG4105CK6 Bacteria
COG0745 LUCA
HOGENOMiHOG000034819
InParanoidiP0AFJ5
KOiK07657
PhylomeDBiP0AFJ5

Enzyme and pathway databases

BioCyciEcoCyc:PHOB-MONOMER
ECOL316407:JW0389-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AFJ5

Protein Ontology

More...
PROi
PR:P0AFJ5

Family and domain databases

CDDicd00156 REC, 1 hit
cd00383 trans_reg_C, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR001867 OmpR/PhoB-type_DNA-bd
IPR011879 Sig_transdc_resp-reg_PhoB
IPR001789 Sig_transdc_resp-reg_receiver
IPR039420 WalR-like
IPR036388 WH-like_DNA-bd_sf
PANTHERiPTHR26402 PTHR26402, 1 hit
PfamiView protein in Pfam
PF00072 Response_reg, 1 hit
PF00486 Trans_reg_C, 1 hit
SMARTiView protein in SMART
SM00448 REC, 1 hit
SM00862 Trans_reg_C, 1 hit
SUPFAMiSSF52172 SSF52172, 1 hit
TIGRFAMsiTIGR02154 PhoB, 1 hit
PROSITEiView protein in PROSITE
PS51755 OMPR_PHOB, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPHOB_ECOLI
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AFJ5
Secondary accession number(s): P08402, Q2MC27
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: May 8, 2019
This is version 112 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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