UniProtKB - P0AFI2 (PARC_ECOLI)
Protein
DNA topoisomerase 4 subunit A
Gene
parC
Organism
Escherichia coli (strain K12)
Status
Functioni
Topoisomerase IV is essential for chromosome segregation; it is the principal protein responsible for decatenating newly replicated chromosomes (PubMed:9334322). It relaxes supercoiled DNA (PubMed:12269820, PuMed:16023670, PubMed:21300644). MukB stimulates the relaxation activity of topoisomerase IV and also has a modest effect on decatenation (PubMed:20921377).5 Publications
Catalytic activityi
- ATP-dependent breakage, passage and rejoining of double-stranded DNA.UniRule annotation3 Publications EC:5.6.2.2
Activity regulationi
Inhibited by quinolones.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 119 | Transition state stabilizerUniRule annotation | 1 | |
Active sitei | 120 | O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: GO_Central
- DNA binding Source: EcoliWiki
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity Source: UniProtKB-UniRule
GO - Biological processi
- chromosome segregation Source: EcoliWiki
- DNA topological change Source: EcoliWiki
- plasmid partitioning Source: EcoliWiki
- sister chromatid cohesion Source: EcoliWiki
Keywordsi
Molecular function | DNA-binding, Isomerase, Topoisomerase |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10686-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: DNA topoisomerase 4 subunit AUniRule annotation (EC:5.6.2.2UniRule annotation3 Publications)Alternative name(s): Topoisomerase IV subunit AUniRule annotation |
Gene namesi | Name:parCUniRule annotation Ordered Locus Names:b3019, JW2987 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Cytosol
- cytosol Source: EcoCyc
Plasma Membrane
- extrinsic component of plasma membrane Source: EcoliWiki
Other locations
- chromosome Source: InterPro
- cytoplasm Source: EcoliWiki
- DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex Source: EcoliWiki
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 80 | S → L: Confers resistance to quinolones. No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 84 | E → K: Strongly reduced enzyme activity. Increases stability of covalent reaction intermediate with DNA. Confers resistance to quinolones. 1 Publication | 1 | |
Mutagenesisi | 84 | E → P: Confers resistance to quinolones. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL1895 |
DrugBanki | DB11943, Delafloxacin DB12924, Ozenoxacin DB00817, Rosoxacin |
DrugCentrali | P0AFI2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145397 | 1 – 752 | DNA topoisomerase 4 subunit AAdd BLAST | 752 |
Proteomic databases
jPOSTi | P0AFI2 |
PaxDbi | P0AFI2 |
PRIDEi | P0AFI2 |
Interactioni
Subunit structurei
Heterotetramer composed of ParC and ParE.
Interacts with MukB.
UniRule annotation4 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 39 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 75 | Interaction with DNAUniRule annotation | 1 | |
Sitei | 77 | Interaction with DNAUniRule annotation | 1 |
Binary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 4262365, 18 interactors |
ComplexPortali | CPX-1104, Topoisomerase IV |
DIPi | DIP-36030N |
IntActi | P0AFI2, 24 interactors |
MINTi | P0AFI2 |
STRINGi | 511145.b3019 |
Chemistry databases
BindingDBi | P0AFI2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFI2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFI2 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 497 – 752 | Sufficient for MukB bindingAdd BLAST | 256 |
Sequence similaritiesi
Belongs to the type II topoisomerase GyrA/ParC subunit family. ParC type 1 subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0188, Bacteria |
HOGENOMi | CLU_002977_6_1_6 |
InParanoidi | P0AFI2 |
PhylomeDBi | P0AFI2 |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_00936, ParC_type1, 1 hit |
InterProi | View protein in InterPro IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf IPR005742, TopoIV_A_Gneg |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 2 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFI2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSDMAERLAL HEFTENAYLN YSMYVIMDRA LPFIGDGLKP VQRRIVYAMS
60 70 80 90 100
ELGLNASAKF KKSARTVGDV LGKYHPHGDS ACYEAMVLMA QPFSYRYPLV
110 120 130 140 150
DGQGNWGAPD DPKSFAAMRY TESRLSKYSE LLLSELGQGT ADWVPNFDGT
160 170 180 190 200
LQEPKMLPAR LPNILLNGTT GIAVGMATDI PPHNLREVAQ AAIALIDQPK
210 220 230 240 250
TTLDQLLDIV QGPDYPTEAE IITSRAEIRK IYENGRGSVR MRAVWKKEDG
260 270 280 290 300
AVVISALPHQ VSGARVLEQI AAQMRNKKLP MVDDLRDESD HENPTRLVIV
310 320 330 340 350
PRSNRVDMDQ VMNHLFATTD LEKSYRINLN MIGLDGRPAV KNLLEILSEW
360 370 380 390 400
LVFRRDTVRR RLNYRLEKVL KRLHILEGLL VAFLNIDEVI EIIRNEDEPK
410 420 430 440 450
PALMSRFGLT ETQAEAILEL KLRHLAKLEE MKIRGEQSEL EKERDQLQGI
460 470 480 490 500
LASERKMNNL LKKELQADAQ AYGDDRRSPL QEREEAKAMS EHDMLPSEPV
510 520 530 540 550
TIVLSQMGWV RSAKGHDIDA PGLNYKAGDS FKAAVKGKSN QPVVFVDSTG
560 570 580 590 600
RSYAIDPITL PSARGQGEPL TGKLTLPPGA TVDHMLMESD DQKLLMASDA
610 620 630 640 650
GYGFVCTFND LVARNRAGKA LITLPENAHV MPPVVIEDAS DMLLAITQAG
660 670 680 690 700
RMLMFPVSDL PQLSKGKGNK IINIPSAEAA RGEDGLAQLY VLPPQSTLTI
710 720 730 740 750
HVGKRKIKLR PEELQKVTGE RGRRGTLMRG LQRIDRVEID SPRRASSGDS
EE
Sequence cautioni
The sequence AAA24297 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58408 Genomic DNA Translation: AAA24297.1 Frameshift. M63491 Genomic DNA Translation: AAA24396.1 U28377 Genomic DNA Translation: AAA69187.1 U00096 Genomic DNA Translation: AAC76055.1 AP009048 Genomic DNA Translation: BAE77075.1 L22025 Unassigned DNA Translation: AAC36840.1 |
PIRi | A65089 |
RefSeqi | NP_417491.1, NC_000913.3 WP_001281881.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC76055; AAC76055; b3019 BAE77075; BAE77075; BAE77075 |
GeneIDi | 58460630 947499 |
KEGGi | ecj:JW2987 eco:b3019 |
PATRICi | fig|1411691.4.peg.3711 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M58408 Genomic DNA Translation: AAA24297.1 Frameshift. M63491 Genomic DNA Translation: AAA24396.1 U28377 Genomic DNA Translation: AAA69187.1 U00096 Genomic DNA Translation: AAC76055.1 AP009048 Genomic DNA Translation: BAE77075.1 L22025 Unassigned DNA Translation: AAC36840.1 |
PIRi | A65089 |
RefSeqi | NP_417491.1, NC_000913.3 WP_001281881.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ZVT | X-ray | 1.70 | A/B | 497-752 | [»] | |
1ZVU | X-ray | 3.00 | A | 27-742 | [»] | |
4MN4 | X-ray | 2.30 | A/B | 497-752 | [»] | |
SMRi | P0AFI2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262365, 18 interactors |
ComplexPortali | CPX-1104, Topoisomerase IV |
DIPi | DIP-36030N |
IntActi | P0AFI2, 24 interactors |
MINTi | P0AFI2 |
STRINGi | 511145.b3019 |
Chemistry databases
BindingDBi | P0AFI2 |
ChEMBLi | CHEMBL1895 |
DrugBanki | DB11943, Delafloxacin DB12924, Ozenoxacin DB00817, Rosoxacin |
DrugCentrali | P0AFI2 |
Proteomic databases
jPOSTi | P0AFI2 |
PaxDbi | P0AFI2 |
PRIDEi | P0AFI2 |
Genome annotation databases
EnsemblBacteriai | AAC76055; AAC76055; b3019 BAE77075; BAE77075; BAE77075 |
GeneIDi | 58460630 947499 |
KEGGi | ecj:JW2987 eco:b3019 |
PATRICi | fig|1411691.4.peg.3711 |
Organism-specific databases
EchoBASEi | EB0680 |
Phylogenomic databases
eggNOGi | COG0188, Bacteria |
HOGENOMi | CLU_002977_6_1_6 |
InParanoidi | P0AFI2 |
PhylomeDBi | P0AFI2 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10686-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFI2 |
PROi | PR:P0AFI2 |
Family and domain databases
CDDi | cd00187, TOP4c, 1 hit |
Gene3Di | 1.10.268.10, 1 hit 2.120.10.90, 1 hit 3.90.199.10, 1 hit |
HAMAPi | MF_00936, ParC_type1, 1 hit |
InterProi | View protein in InterPro IPR006691, GyrA/parC_rep IPR035516, Gyrase/topoIV_suA_C IPR013760, Topo_IIA-like_dom_sf IPR002205, Topo_IIA_A/C IPR013758, Topo_IIA_A/C_ab IPR013757, Topo_IIA_A_a_sf IPR005742, TopoIV_A_Gneg |
Pfami | View protein in Pfam PF03989, DNA_gyraseA_C, 2 hits PF00521, DNA_topoisoIV, 1 hit |
SMARTi | View protein in SMART SM00434, TOP4c, 1 hit |
SUPFAMi | SSF101904, SSF101904, 1 hit SSF56719, SSF56719, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PARC_ECOLI | |
Accessioni | P0AFI2Primary (citable) accession number: P0AFI2 Secondary accession number(s): O69154, P20082, Q2M9I1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families