We will be switching to the new UniProt website on Tuesday, June 28. Please explore and share your feedback.
Take me to UniProt BETA
UniProtKB - P0AFH0 (OGT_ECOLI)
Protein
Methylated-DNA--protein-cysteine methyltransferase
Gene
ogt
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Miscellaneous
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.
Catalytic activityi
- a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]UniRule annotationEC:2.1.1.63UniRule annotation
- a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]UniRule annotationEC:2.1.1.63UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 139 | Nucleophile; methyl group acceptorCurated | 1 |
GO - Molecular functioni
GO - Biological processi
- DNA dealkylation involved in DNA repair Source: EcoCyc
- methylation Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | DNA damage, DNA repair |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10668-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)Alternative name(s): 6-O-methylguanine-DNA methyltransferaseUniRule annotation Short name: MGMTUniRule annotation O-6-methylguanine-DNA-alkyltransferaseUniRule annotation |
Gene namesi | Name:ogtUniRule annotation Ordered Locus Names:b1335, JW1329 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 102 | C → G: Little effect on the activity. 1 Publication | 1 | |
Mutagenesisi | 139 | C → G: Eliminates the activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000139365 | 1 – 171 | Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST | 171 |
Proteomic databases
jPOSTi | P0AFH0 |
PaxDbi | P0AFH0 |
PRIDEi | P0AFH0 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262876, 110 interactors |
DIPi | DIP-48073N |
IntActi | P0AFH0, 6 interactors |
STRINGi | 511145.b1335 |
Family & Domainsi
Sequence similaritiesi
Belongs to the MGMT family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0350, Bacteria |
HOGENOMi | CLU_000445_52_2_6 |
InParanoidi | P0AFH0 |
OMAi | SACAMNA |
PhylomeDBi | P0AFH0 |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00772, OGT, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR023546, MGMT IPR036631, MGMT_N_sf IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit SSF53155, SSF53155, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFH0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLRLLEEKIA TPLGPLWVIC DEQFRLRAVE WEEYSERMVQ LLDIHYRKEG
60 70 80 90 100
YERISATNPG GLSDKLREYF AGNLSIIDTL PTATGGTPFQ REVWKTLRTI
110 120 130 140 150
PCGQVMHYGQ LAEQLGRPGA ARAVGAANGS NPISIVVPCH RVIGRNGTMT
160 170
GYAGGVQRKE WLLRHEGYLL L
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 37 | R → A in CAA68548 (PubMed:2825131).Curated | 1 | |
Sequence conflicti | 68 | E → D in CAA68548 (PubMed:2825131).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00495 Genomic DNA Translation: CAA68548.1 U00096 Genomic DNA Translation: AAC74417.1 AP009048 Genomic DNA Translation: BAA14928.1 |
PIRi | B64883, XUECAD |
RefSeqi | NP_415851.1, NC_000913.3 WP_000945011.1, NZ_SSZK01000012.1 |
Genome annotation databases
EnsemblBacteriai | AAC74417; AAC74417; b1335 BAA14928; BAA14928; BAA14928 |
GeneIDi | 58391829 945853 |
KEGGi | ecj:JW1329 eco:b1335 |
PATRICi | fig|1411691.4.peg.942 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y00495 Genomic DNA Translation: CAA68548.1 U00096 Genomic DNA Translation: AAC74417.1 AP009048 Genomic DNA Translation: BAA14928.1 |
PIRi | B64883, XUECAD |
RefSeqi | NP_415851.1, NC_000913.3 WP_000945011.1, NZ_SSZK01000012.1 |
3D structure databases
AlphaFoldDBi | P0AFH0 |
SMRi | P0AFH0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262876, 110 interactors |
DIPi | DIP-48073N |
IntActi | P0AFH0, 6 interactors |
STRINGi | 511145.b1335 |
Proteomic databases
jPOSTi | P0AFH0 |
PaxDbi | P0AFH0 |
PRIDEi | P0AFH0 |
Genome annotation databases
EnsemblBacteriai | AAC74417; AAC74417; b1335 BAA14928; BAA14928; BAA14928 |
GeneIDi | 58391829 945853 |
KEGGi | ecj:JW1329 eco:b1335 |
PATRICi | fig|1411691.4.peg.942 |
Organism-specific databases
EchoBASEi | EB0662 |
Phylogenomic databases
eggNOGi | COG0350, Bacteria |
HOGENOMi | CLU_000445_52_2_6 |
InParanoidi | P0AFH0 |
OMAi | SACAMNA |
PhylomeDBi | P0AFH0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10668-MONOMER |
Miscellaneous databases
PROi | PR:P0AFH0 |
Family and domain databases
CDDi | cd06445, ATase, 1 hit |
Gene3Di | 1.10.10.10, 1 hit |
HAMAPi | MF_00772, OGT, 1 hit |
InterProi | View protein in InterPro IPR001497, MethylDNA_cys_MeTrfase_AS IPR014048, MethylDNA_cys_MeTrfase_DNA-bd IPR036217, MethylDNA_cys_MeTrfase_DNAb IPR008332, MethylG_MeTrfase_N IPR023546, MGMT IPR036631, MGMT_N_sf IPR036388, WH-like_DNA-bd_sf |
Pfami | View protein in Pfam PF01035, DNA_binding_1, 1 hit PF02870, Methyltransf_1N, 1 hit |
SUPFAMi | SSF46767, SSF46767, 1 hit SSF53155, SSF53155, 1 hit |
TIGRFAMsi | TIGR00589, ogt, 1 hit |
PROSITEi | View protein in PROSITE PS00374, MGMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | OGT_ECOLI | |
Accessioni | P0AFH0Primary (citable) accession number: P0AFH0 Secondary accession number(s): P09168, P78284 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families