UniProtKB - P0AFG8 (ODP1_ECOLI)
Protein
Pyruvate dehydrogenase E1 component
Gene
aceE
Organism
Escherichia coli (strain K12)
Status
Functioni
Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2.
Catalytic activityi
- [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-lipoyl-L-lysine + H+ + pyruvate = [dihydrolipoyllysine-residue acetyltransferase]-(R)-N6-(S8-acetyldihydrolipoyl)-L-lysine + CO2EC:1.2.4.1
Cofactori
Protein has several cofactor binding sites:Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 231 | Magnesium | 1 | |
Metal bindingi | 261 | Magnesium | 1 | |
Metal bindingi | 263 | Magnesium; via carbonyl oxygen | 1 |
GO - Molecular functioni
- identical protein binding Source: IntAct
- magnesium ion binding Source: CAFA
- protein homodimerization activity Source: CAFA
- pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC
- pyruvate dehydrogenase activity Source: EcoliWiki
- thiamine pyrophosphate binding Source: CAFA
GO - Biological processi
- glycolytic process Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding, Pyruvate, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:E1P-MONOMER MetaCyc:E1P-MONOMER |
BRENDAi | 1.2.4.1, 2026 |
SABIO-RKi | P0AFG8 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:aceE Ordered Locus Names:b0114, JW0110 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- cytosol Source: UniProtKB
- cytosolic pyruvate dehydrogenase complex Source: EcoCyc
- membrane Source: UniProtKB
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000162243 | 2 – 887 | Pyruvate dehydrogenase E1 componentAdd BLAST | 886 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 716 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0AFG8 |
PaxDbi | P0AFG8 |
PRIDEi | P0AFG8 |
2D gel databases
SWISS-2DPAGEi | P0AFG8 |
PTM databases
CarbonylDBi | P0AFG8 |
iPTMneti | P0AFG8 |
Interactioni
Subunit structurei
Homodimer. Part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Binary interactionsi
Hide detailsP0AFG8
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein homodimerization activity Source: CAFA
Protein-protein interaction databases
BioGRIDi | 4261303, 14 interactors 849234, 1 interactor |
ComplexPortali | CPX-3943, Pyruvate dehydrogenase complex |
DIPi | DIP-9039N |
IntActi | P0AFG8, 104 interactors |
STRINGi | 511145.b0114 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFG8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFG8 |
Family & Domainsi
Phylogenomic databases
eggNOGi | COG2609, Bacteria |
HOGENOMi | CLU_009154_2_0_6 |
InParanoidi | P0AFG8 |
PhylomeDBi | P0AFG8 |
Family and domain databases
CDDi | cd02017, TPP_E1_EcPDC_like, 1 hit |
DisProti | DP00427 |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR035807, PDC_E1_N IPR004660, PDH_E1 IPR041621, PDH_E1_M IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005474, Transketolase_N |
PANTHERi | PTHR43825:SF3, PTHR43825:SF3, 1 hit |
Pfami | View protein in Pfam PF17831, PDH_E1_M, 1 hit PF00456, Transketolase_N, 1 hit |
PIRSFi | PIRSF000156, Pyruvate_dh_E1, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00759, aceE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AFG8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSERFPNDVD PIETRDWLQA IESVIREEGV ERAQYLIDQL LAEARKGGVN
60 70 80 90 100
VAAGTGISNY INTIPVEEQP EYPGNLELER RIRSAIRWNA IMTVLRASKK
110 120 130 140 150
DLELGGHMAS FQSSATIYDV CFNHFFRARN EQDGGDLVYF QGHISPGVYA
160 170 180 190 200
RAFLEGRLTQ EQLDNFRQEV HGNGLSSYPH PKLMPEFWQF PTVSMGLGPI
210 220 230 240 250
GAIYQAKFLK YLEHRGLKDT SKQTVYAFLG DGEMDEPESK GAITIATREK
260 270 280 290 300
LDNLVFVINC NLQRLDGPVT GNGKIINELE GIFEGAGWNV IKVMWGSRWD
310 320 330 340 350
ELLRKDTSGK LIQLMNETVD GDYQTFKSKD GAYVREHFFG KYPETAALVA
360 370 380 390 400
DWTDEQIWAL NRGGHDPKKI YAAFKKAQET KGKATVILAH TIKGYGMGDA
410 420 430 440 450
AEGKNIAHQV KKMNMDGVRH IRDRFNVPVS DADIEKLPYI TFPEGSEEHT
460 470 480 490 500
YLHAQRQKLH GYLPSRQPNF TEKLELPSLQ DFGALLEEQS KEISTTIAFV
510 520 530 540 550
RALNVMLKNK SIKDRLVPII ADEARTFGME GLFRQIGIYS PNGQQYTPQD
560 570 580 590 600
REQVAYYKED EKGQILQEGI NELGAGCSWL AAATSYSTNN LPMIPFYIYY
610 620 630 640 650
SMFGFQRIGD LCWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS
660 670 680 690 700
LTIPNCISYD PAYAYEVAVI MHDGLERMYG EKQENVYYYI TTLNENYHMP
710 720 730 740 750
AMPEGAEEGI RKGIYKLETI EGSKGKVQLL GSGSILRHVR EAAEILAKDY
760 770 780 790 800
GVGSDVYSVT SFTELARDGQ DCERWNMLHP LETPRVPYIA QVMNDAPAVA
810 820 830 840 850
STDYMKLFAE QVRTYVPADD YRVLGTDGFG RSDSRENLRH HFEVDASYVV
860 870 880
VAALGELAKR GEIDKKVVAD AIAKFNIDAD KVNPRLA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 146 | P → R in CAA24740 (PubMed:6343085).Curated | 1 | |
Sequence conflicti | 276 | Missing in CAA24740 (PubMed:6343085).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01498 Genomic DNA Translation: CAA24740.1 U00096 Genomic DNA Translation: AAC73225.1 AP009048 Genomic DNA Translation: BAB96684.2 S67363 Genomic DNA Translation: AAB29357.1 |
PIRi | B64734, DEECPV |
RefSeqi | NP_414656.1, NC_000913.3 WP_000003820.1, NZ_STEB01000010.1 |
Genome annotation databases
EnsemblBacteriai | AAC73225; AAC73225; b0114 BAB96684; BAB96684; BAB96684 |
GeneIDi | 944834 |
KEGGi | ecj:JW0110 eco:b0114 |
PATRICi | fig|1411691.4.peg.2168 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01498 Genomic DNA Translation: CAA24740.1 U00096 Genomic DNA Translation: AAC73225.1 AP009048 Genomic DNA Translation: BAB96684.2 S67363 Genomic DNA Translation: AAB29357.1 |
PIRi | B64734, DEECPV |
RefSeqi | NP_414656.1, NC_000913.3 WP_000003820.1, NZ_STEB01000010.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1L8A | X-ray | 1.85 | A/B | 2-887 | [»] | |
1RP7 | X-ray | 2.09 | A/B | 2-887 | [»] | |
2G25 | X-ray | 2.10 | A/B | 2-887 | [»] | |
2G28 | X-ray | 1.85 | A/B | 2-887 | [»] | |
2G67 | X-ray | 2.32 | A/B | 2-887 | [»] | |
2IEA | X-ray | 1.85 | A/B | 2-887 | [»] | |
2QTA | X-ray | 1.85 | A/B | 2-887 | [»] | |
2QTC | X-ray | 1.77 | A/B | 2-887 | [»] | |
SMRi | P0AFG8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261303, 14 interactors 849234, 1 interactor |
ComplexPortali | CPX-3943, Pyruvate dehydrogenase complex |
DIPi | DIP-9039N |
IntActi | P0AFG8, 104 interactors |
STRINGi | 511145.b0114 |
Chemistry databases
DrugBanki | DB01987, Cocarboxylase |
PTM databases
CarbonylDBi | P0AFG8 |
iPTMneti | P0AFG8 |
2D gel databases
SWISS-2DPAGEi | P0AFG8 |
Proteomic databases
jPOSTi | P0AFG8 |
PaxDbi | P0AFG8 |
PRIDEi | P0AFG8 |
Genome annotation databases
EnsemblBacteriai | AAC73225; AAC73225; b0114 BAB96684; BAB96684; BAB96684 |
GeneIDi | 944834 |
KEGGi | ecj:JW0110 eco:b0114 |
PATRICi | fig|1411691.4.peg.2168 |
Organism-specific databases
EchoBASEi | EB0023 |
Phylogenomic databases
eggNOGi | COG2609, Bacteria |
HOGENOMi | CLU_009154_2_0_6 |
InParanoidi | P0AFG8 |
PhylomeDBi | P0AFG8 |
Enzyme and pathway databases
BioCyci | EcoCyc:E1P-MONOMER MetaCyc:E1P-MONOMER |
BRENDAi | 1.2.4.1, 2026 |
SABIO-RKi | P0AFG8 |
Miscellaneous databases
EvolutionaryTracei | P0AFG8 |
PROi | PR:P0AFG8 |
Family and domain databases
CDDi | cd02017, TPP_E1_EcPDC_like, 1 hit |
DisProti | DP00427 |
Gene3Di | 3.40.50.920, 1 hit |
InterProi | View protein in InterPro IPR035807, PDC_E1_N IPR004660, PDH_E1 IPR041621, PDH_E1_M IPR029061, THDP-binding IPR009014, Transketo_C/PFOR_II IPR005474, Transketolase_N |
PANTHERi | PTHR43825:SF3, PTHR43825:SF3, 1 hit |
Pfami | View protein in Pfam PF17831, PDH_E1_M, 1 hit PF00456, Transketolase_N, 1 hit |
PIRSFi | PIRSF000156, Pyruvate_dh_E1, 1 hit |
SUPFAMi | SSF52518, SSF52518, 2 hits SSF52922, SSF52922, 1 hit |
TIGRFAMsi | TIGR00759, aceE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ODP1_ECOLI | |
Accessioni | P0AFG8Primary (citable) accession number: P0AFG8 Secondary accession number(s): P06958, P78049, Q53382 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references