UniProtKB - P0AFG6 (ODO2_ECOLI)
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex
sucB
Functioni
Catalytic activityi
- (R)-N6-dihydrolipoyl-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + succinyl-CoA = (R)-N6-(S8-succinyldihydrolipoyl)-L-lysyl-[2-oxoglutarate dehydrogenase complex component E2] + CoA1 PublicationEC:2.3.1.611 Publication
Cofactori
: L-lysine degradation via saccharopine pathway Pathwayi
This protein is involved in step 6 of the subpathway that synthesizes glutaryl-CoA from L-lysine.Proteins known to be involved in the 6 steps of the subpathway in this organism are:
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
- no protein annotated in this organism
- Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (odhB), Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (sucB)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutaryl-CoA from L-lysine, the pathway L-lysine degradation via saccharopine pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 376 | 2 Publications | 1 | |
Active sitei | 380 | 2 Publications | 1 |
GO - Molecular functioni
- dihydrolipoyllysine-residue succinyltransferase activity Source: EcoliWiki
- lipoic acid binding Source: EcoliWiki
GO - Biological processi
- L-lysine catabolic process to acetyl-CoA via saccharopine Source: UniProtKB-UniPathway
- tricarboxylic acid cycle Source: EcoliWiki
Keywordsi
Molecular function | Acyltransferase, Transferase |
Biological process | Tricarboxylic acid cycle |
Enzyme and pathway databases
BioCyci | EcoCyc:E2O-MONOMER MetaCyc:E2O-MONOMER |
BRENDAi | 2.3.1.61, 2026 |
SABIO-RKi | P0AFG6 |
UniPathwayi | UPA00868;UER00840 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC:2.3.1.611 Publication)Alternative name(s): 2-oxoglutarate dehydrogenase complex component E2 Short name: OGDC-E2 Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex |
Gene namesi | Name:sucB Ordered Locus Names:b0727, JW0716 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: EcoCyc
Other locations
- oxoglutarate dehydrogenase complex Source: EcoliWiki
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000162262 | 2 – 405 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexAdd BLAST | 404 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 44 | N6-lipoyllysinePROSITE-ProRule annotation1 Publication | 1 | |
Modified residuei | 148 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0AFG6 |
PaxDbi | P0AFG6 |
PRIDEi | P0AFG6 |
2D gel databases
SWISS-2DPAGEi | P0AFG6 |
PTM databases
iPTMneti | P0AFG6 |
Interactioni
Subunit structurei
Forms a 24-polypeptide structural core with octahedral symmetry (PubMed:9677295, PubMed:10739245). Part of the 2-oxoglutarate dehydrogenase (OGDH) complex composed of E1 (2-oxoglutarate dehydrogenase), E2 (dihydrolipoamide succinyltransferase) and E3 (dihydrolipoamide dehydrogenase); the complex contains multiple copies of the three enzymatic components (E1, E2 and E3) (Probable).
Interacts with SucA (via N-terminus), the E1 component of OGDH complex (PubMed:17367808).
1 Publication3 PublicationsBinary interactionsi
Hide detailsP0AFG6
With | #Exp. | IntAct |
---|---|---|
lpdA [P0A9P0] | 3 | EBI-558621,EBI-542856 |
sucA [P0AFG3] | 7 | EBI-558621,EBI-543523 |
ybaY [P77717] | 3 | EBI-558621,EBI-558645 |
Protein-protein interaction databases
BioGRIDi | 4259945, 23 interactors 849684, 3 interactors |
ComplexPortali | CPX-3921, 2-oxoglutarate dehydrogenase complex |
DIPi | DIP-35787N |
IntActi | P0AFG6, 39 interactors |
STRINGi | 511145.b0727 |
Structurei
Secondary structure
3D structure databases
BMRBi | P0AFG6 |
SASBDBi | P0AFG6 |
SMRi | P0AFG6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFG6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 78 | Lipoyl-bindingPROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 113 – 150 | Peripheral subunit-binding (PSBD)PROSITE-ProRule annotationAdd BLAST | 38 |
Sequence similaritiesi
Keywords - Domaini
LipoylPhylogenomic databases
eggNOGi | COG0508, Bacteria |
HOGENOMi | CLU_016733_0_0_6 |
InParanoidi | P0AFG6 |
PhylomeDBi | P0AFG6 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR036625, E3-bd_dom_sf IPR004167, PSBD IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit PS51826, PSBD, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSSVDILVPD LPESVADATV ATWHKKPGDA VVRDEVLVEI ETDKVVLEVP
60 70 80 90 100
ASADGILDAV LEDEGTTVTS RQILGRLREG NSAGKETSAK SEEKASTPAQ
110 120 130 140 150
RQQASLEEQN NDALSPAIRR LLAEHNLDAS AIKGTGVGGR LTREDVEKHL
160 170 180 190 200
AKAPAKESAP AAAAPAAQPA LAARSEKRVP MTRLRKRVAE RLLEAKNSTA
210 220 230 240 250
MLTTFNEVNM KPIMDLRKQY GEAFEKRHGI RLGFMSFYVK AVVEALKRYP
260 270 280 290 300
EVNASIDGDD VVYHNYFDVS MAVSTPRGLV TPVLRDVDTL GMADIEKKIK
310 320 330 340 350
ELAVKGRDGK LTVEDLTGGN FTITNGGVFG SLMSTPIINP PQSAILGMHA
360 370 380 390 400
IKDRPMAVNG QVEILPMMYL ALSYDHRLID GRESVGFLVT IKELLEDPTR
LLLDV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01619 Genomic DNA Translation: AAA23898.1 X00664 Genomic DNA Translation: CAA25284.1 U00096 Genomic DNA Translation: AAC73821.1 AP009048 Genomic DNA Translation: BAA35393.1 X00661 Genomic DNA Translation: CAA25281.1 |
PIRi | F64808, XUECSD |
RefSeqi | NP_415255.1, NC_000913.3 WP_000099823.1, NZ_SSZK01000033.1 |
Genome annotation databases
EnsemblBacteriai | AAC73821; AAC73821; b0727 BAA35393; BAA35393; BAA35393 |
GeneIDi | 58460903 945307 |
KEGGi | ecj:JW0716 eco:b0727 |
PATRICi | fig|1411691.4.peg.1546 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01619 Genomic DNA Translation: AAA23898.1 X00664 Genomic DNA Translation: CAA25284.1 U00096 Genomic DNA Translation: AAC73821.1 AP009048 Genomic DNA Translation: BAA35393.1 X00661 Genomic DNA Translation: CAA25281.1 |
PIRi | F64808, XUECSD |
RefSeqi | NP_415255.1, NC_000913.3 WP_000099823.1, NZ_SSZK01000033.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BAL | NMR | - | A | 104-153 | [»] | |
1BBL | NMR | - | A | 104-153 | [»] | |
1C4T | X-ray | 3.00 | A/B/C | 173-405 | [»] | |
1E2O | X-ray | 3.00 | A | 173-405 | [»] | |
1PMR | NMR | - | A | 2-81 | [»] | |
1SCZ | X-ray | 2.20 | A | 173-405 | [»] | |
1W4H | NMR | - | A | 109-153 | [»] | |
2BTG | NMR | - | A | 109-153 | [»] | |
2BTH | NMR | - | A | 109-153 | [»] | |
2WXC | NMR | - | A | 109-153 | [»] | |
6PBR | X-ray | 3.00 | A/B/C/D/E/F | 173-405 | [»] | |
BMRBi | P0AFG6 | |||||
SASBDBi | P0AFG6 | |||||
SMRi | P0AFG6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259945, 23 interactors 849684, 3 interactors |
ComplexPortali | CPX-3921, 2-oxoglutarate dehydrogenase complex |
DIPi | DIP-35787N |
IntActi | P0AFG6, 39 interactors |
STRINGi | 511145.b0727 |
PTM databases
iPTMneti | P0AFG6 |
2D gel databases
SWISS-2DPAGEi | P0AFG6 |
Proteomic databases
jPOSTi | P0AFG6 |
PaxDbi | P0AFG6 |
PRIDEi | P0AFG6 |
Genome annotation databases
EnsemblBacteriai | AAC73821; AAC73821; b0727 BAA35393; BAA35393; BAA35393 |
GeneIDi | 58460903 945307 |
KEGGi | ecj:JW0716 eco:b0727 |
PATRICi | fig|1411691.4.peg.1546 |
Organism-specific databases
EchoBASEi | EB0973 |
Phylogenomic databases
eggNOGi | COG0508, Bacteria |
HOGENOMi | CLU_016733_0_0_6 |
InParanoidi | P0AFG6 |
PhylomeDBi | P0AFG6 |
Enzyme and pathway databases
UniPathwayi | UPA00868;UER00840 |
BioCyci | EcoCyc:E2O-MONOMER MetaCyc:E2O-MONOMER |
BRENDAi | 2.3.1.61, 2026 |
SABIO-RKi | P0AFG6 |
Miscellaneous databases
EvolutionaryTracei | P0AFG6 |
PROi | PR:P0AFG6 |
Family and domain databases
Gene3Di | 3.30.559.10, 1 hit 4.10.320.10, 1 hit |
InterProi | View protein in InterPro IPR003016, 2-oxoA_DH_lipoyl-BS IPR001078, 2-oxoacid_DH_actylTfrase IPR000089, Biotin_lipoyl IPR023213, CAT-like_dom_sf IPR036625, E3-bd_dom_sf IPR004167, PSBD IPR011053, Single_hybrid_motif IPR006255, SucB |
Pfami | View protein in Pfam PF00198, 2-oxoacid_dh, 1 hit PF00364, Biotin_lipoyl, 1 hit PF02817, E3_binding, 1 hit |
SUPFAMi | SSF47005, SSF47005, 1 hit SSF51230, SSF51230, 1 hit |
TIGRFAMsi | TIGR01347, sucB, 1 hit |
PROSITEi | View protein in PROSITE PS50968, BIOTINYL_LIPOYL, 1 hit PS00189, LIPOYL, 1 hit PS51826, PSBD, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ODO2_ECOLI | |
Accessioni | P0AFG6Primary (citable) accession number: P0AFG6 Secondary accession number(s): P07016 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 1, 1988 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families