UniProtKB - P0AFG0 (NUSG_ECOLI)
Transcription termination/antitermination protein NusG
nusG
Functioni
Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed:32871103).
Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNAP, probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.
UniRule annotation10 PublicationsGO - Biological processi
- DNA-templated transcription, elongation Source: UniProtKB-UniRule
- DNA-templated transcription, termination Source: EcoCyc
- regulation of DNA-templated transcription, elongation Source: InterPro
- ribosome biogenesis Source: UniProtKB-KW
- transcription antitermination Source: EcoCyc
Keywordsi
Biological process | Ribosome biogenesis, Transcription, Transcription antitermination, Transcription regulation, Transcription termination |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10667-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription termination/antitermination protein NusGUniRule annotation |
Gene namesi | Name:nusGUniRule annotation Ordered Locus Names:b3982, JW3945 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000113926 | 2 – 181 | Transcription termination/antitermination protein NusGAdd BLAST | 180 |
Proteomic databases
jPOSTi | P0AFG0 |
PaxDbi | P0AFG0 |
PRIDEi | P0AFG0 |
2D gel databases
SWISS-2DPAGEi | P0AFG0 |
Interactioni
Subunit structurei
Monomer.
Interacts with the transcription termination factor Rho and with RNAP. One NusG monomer forms a stable complex with a Rho hexamer. Binds directly, but weakly, to the core enzyme of RNAP.
Also interacts with RpsJ (NusE). The rRNA transcription and antitermination complex (rrnTAC) consists of RNAP, NusA, NusB, NusE (rpsJ), NusG, SubB, ribosomal protein S4, DNA and precursor rRNA; S4 is more flexible than other subunits (PubMed:32871103).
UniRule annotation5 PublicationsBinary interactionsi
Protein-protein interaction databases
BioGRIDi | 4259510, 11 interactors 852779, 4 interactors |
ComplexPortali | CPX-5674, Transcription elongation complex CPX-5780, lambdaN-dependent processive transcription antitermination complex |
DIPi | DIP-31860N |
IntActi | P0AFG0, 61 interactors |
MINTi | P0AFG0 |
STRINGi | 511145.b3982 |
Structurei
Secondary structure
3D structure databases
BMRBi | P0AFG0 |
SMRi | P0AFG0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFG0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 130 – 161 | KOWUniRule annotationAdd BLAST | 32 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0250, Bacteria |
HOGENOMi | CLU_067287_1_0_6 |
InParanoidi | P0AFG0 |
PhylomeDBi | P0AFG0 |
Family and domain databases
DisProti | DP01496 |
Gene3Di | 2.30.30.30, 1 hit 3.30.70.940, 1 hit |
HAMAPi | MF_00948, NusG, 1 hit |
InterProi | View protein in InterPro IPR005824, KOW IPR006645, NGN_dom IPR036735, NGN_dom_sf IPR043425, NusG-like IPR014722, Rib_L2_dom2 IPR001062, Transcrpt_antiterm_NusG IPR015869, Transcrpt_antiterm_NusG_bac_CS IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR30265, PTHR30265, 1 hit PTHR30265:SF2, PTHR30265:SF2, 1 hit |
Pfami | View protein in Pfam PF00467, KOW, 1 hit PF02357, NusG, 1 hit |
PRINTSi | PR00338, NUSGTNSCPFCT |
SMARTi | View protein in SMART SM00739, KOW, 1 hit SM00738, NGN, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit SSF82679, SSF82679, 1 hit |
TIGRFAMsi | TIGR00922, nusG, 1 hit |
PROSITEi | View protein in PROSITE PS01014, NUSG, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV
60 70 80 90 100
EIRGGQRRKS ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP
110 120 130 140 150
APISDKEVDA IMNRLQQVGD KPRPKTLFEP GEMVRVNDGP FADFNGVVEE
160 170 180
VDYEKSRLKV SVSIFGRATP VELDFSQVEK A
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30610 Genomic DNA Translation: AAA24622.1 U00006 Genomic DNA Translation: AAC43080.1 U00096 Genomic DNA Translation: AAC76956.1 AP009048 Genomic DNA Translation: BAE77338.1 |
PIRi | B35139, TWECNG |
RefSeqi | NP_418409.1, NC_000913.3 WP_001287516.1, NZ_STEB01000045.1 |
Genome annotation databases
EnsemblBacteriai | AAC76956; AAC76956; b3982 BAE77338; BAE77338; BAE77338 |
GeneIDi | 66672107 948485 |
KEGGi | ecj:JW3945 eco:b3982 |
PATRICi | fig|1411691.4.peg.2730 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M30610 Genomic DNA Translation: AAA24622.1 U00006 Genomic DNA Translation: AAC43080.1 U00096 Genomic DNA Translation: AAC76956.1 AP009048 Genomic DNA Translation: BAE77338.1 |
PIRi | B35139, TWECNG |
RefSeqi | NP_418409.1, NC_000913.3 WP_001287516.1, NZ_STEB01000045.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2JVV | NMR | - | A | 1-181 | [»] | |
2K06 | NMR | - | A | 1-123 | [»] | |
2KVQ | NMR | - | G | 123-181 | [»] | |
5MS0 | electron microscopy | 9.80 | F | 1-181 | [»] | |
6C6U | electron microscopy | 3.70 | N | 1-181 | [»] | |
6DUQ | X-ray | 3.70 | M/N/O/P/Q/R/S/T/U/V/W/X | 125-181 | [»] | |
6TQN | electron microscopy | 3.80 | G | 1-181 | [»] | |
6TQO | electron microscopy | 4.00 | G | 1-181 | [»] | |
6XAV | electron microscopy | 7.70 | L | 1-181 | [»] | |
6XE0 | electron microscopy | 6.80 | V | 1-181 | [»] | |
BMRBi | P0AFG0 | |||||
SMRi | P0AFG0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259510, 11 interactors 852779, 4 interactors |
ComplexPortali | CPX-5674, Transcription elongation complex CPX-5780, lambdaN-dependent processive transcription antitermination complex |
DIPi | DIP-31860N |
IntActi | P0AFG0, 61 interactors |
MINTi | P0AFG0 |
STRINGi | 511145.b3982 |
2D gel databases
SWISS-2DPAGEi | P0AFG0 |
Proteomic databases
jPOSTi | P0AFG0 |
PaxDbi | P0AFG0 |
PRIDEi | P0AFG0 |
Genome annotation databases
EnsemblBacteriai | AAC76956; AAC76956; b3982 BAE77338; BAE77338; BAE77338 |
GeneIDi | 66672107 948485 |
KEGGi | ecj:JW3945 eco:b3982 |
PATRICi | fig|1411691.4.peg.2730 |
Organism-specific databases
EchoBASEi | EB0661 |
Phylogenomic databases
eggNOGi | COG0250, Bacteria |
HOGENOMi | CLU_067287_1_0_6 |
InParanoidi | P0AFG0 |
PhylomeDBi | P0AFG0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10667-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AFG0 |
PROi | PR:P0AFG0 |
Family and domain databases
DisProti | DP01496 |
Gene3Di | 2.30.30.30, 1 hit 3.30.70.940, 1 hit |
HAMAPi | MF_00948, NusG, 1 hit |
InterProi | View protein in InterPro IPR005824, KOW IPR006645, NGN_dom IPR036735, NGN_dom_sf IPR043425, NusG-like IPR014722, Rib_L2_dom2 IPR001062, Transcrpt_antiterm_NusG IPR015869, Transcrpt_antiterm_NusG_bac_CS IPR008991, Translation_prot_SH3-like_sf |
PANTHERi | PTHR30265, PTHR30265, 1 hit PTHR30265:SF2, PTHR30265:SF2, 1 hit |
Pfami | View protein in Pfam PF00467, KOW, 1 hit PF02357, NusG, 1 hit |
PRINTSi | PR00338, NUSGTNSCPFCT |
SMARTi | View protein in SMART SM00739, KOW, 1 hit SM00738, NGN, 1 hit |
SUPFAMi | SSF50104, SSF50104, 1 hit SSF82679, SSF82679, 1 hit |
TIGRFAMsi | TIGR00922, nusG, 1 hit |
PROSITEi | View protein in PROSITE PS01014, NUSG, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NUSG_ECOLI | |
Accessioni | P0AFG0Primary (citable) accession number: P0AFG0 Secondary accession number(s): P16921, Q2M8R8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families