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Protein

Transcription termination/antitermination protein NusG

Gene

nusG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNA polymerase (RNAP), probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.UniRule annotation9 Publications

GO - Biological processi

  • DNA-templated transcription, termination Source: EcoCyc
  • regulation of DNA-templated transcription, elongation Source: InterPro
  • transcription antitermination Source: EcoCyc

Keywordsi

Biological processTranscription, Transcription antitermination, Transcription regulation, Transcription termination

Enzyme and pathway databases

BioCyciEcoCyc:EG10667-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription termination/antitermination protein NusGUniRule annotation
Gene namesi
Name:nusGUniRule annotation
Ordered Locus Names:b3982, JW3945
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10667 nusG

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001139262 – 181Transcription termination/antitermination protein NusGAdd BLAST180

Proteomic databases

EPDiP0AFG0
PaxDbiP0AFG0
PRIDEiP0AFG0

2D gel databases

SWISS-2DPAGEiP0AFG0

Interactioni

Subunit structurei

Monomer. Interacts with the transcription termination factor Rho and with RNA polymerase. One NusG monomer forms a stable complex with a Rho hexamer. Binds directly, but weakly, to the core enzyme of RNAP. Also interacts with RpsJ (NusE).UniRule annotation4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4259510, 11 interactors
DIPiDIP-31860N
IntActiP0AFG0, 61 interactors
MINTiP0AFG0
STRINGi316385.ECDH10B_4170

Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi8 – 14Combined sources7
Helixi19 – 33Combined sources15
Turni36 – 38Combined sources3
Beta strandi68 – 73Combined sources6
Helixi77 – 85Combined sources9
Beta strandi86 – 99Combined sources14
Helixi105 – 116Combined sources12
Beta strandi132 – 136Combined sources5
Turni140 – 143Combined sources4
Beta strandi144 – 152Combined sources9
Turni153 – 156Combined sources4
Beta strandi157 – 164Combined sources8
Beta strandi167 – 173Combined sources7
Turni175 – 177Combined sources3
Beta strandi178 – 180Combined sources3

3D structure databases

ProteinModelPortaliP0AFG0
SMRiP0AFG0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AFG0

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini130 – 161KOWUniRule annotationAdd BLAST32

Domaini

The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). These domains are separated by a flexible linker.1 Publication

Sequence similaritiesi

Belongs to the NusG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E5V Bacteria
COG0250 LUCA
HOGENOMiHOG000219264
InParanoidiP0AFG0
KOiK02601
OMAiDGPFNGF
PhylomeDBiP0AFG0

Family and domain databases

Gene3Di2.30.30.30, 1 hit
3.30.70.940, 1 hit
HAMAPiMF_00948 NusG, 1 hit
InterProiView protein in InterPro
IPR005824 KOW
IPR006645 NGN_dom
IPR036735 NGN_dom_sf
IPR014722 Rib_L2_dom2
IPR001062 Transcrpt_antiterm_NusG
IPR015869 Transcrpt_antiterm_NusG_bac_CS
IPR008991 Translation_prot_SH3-like_sf
PANTHERiPTHR30265:SF2 PTHR30265:SF2, 1 hit
PfamiView protein in Pfam
PF00467 KOW, 1 hit
PF02357 NusG, 1 hit
PRINTSiPR00338 NUSGTNSCPFCT
SMARTiView protein in SMART
SM00739 KOW, 1 hit
SM00738 NGN, 1 hit
SUPFAMiSSF50104 SSF50104, 1 hit
SSF82679 SSF82679, 1 hit
TIGRFAMsiTIGR00922 nusG, 1 hit
PROSITEiView protein in PROSITE
PS01014 NUSG, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV
60 70 80 90 100
EIRGGQRRKS ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP
110 120 130 140 150
APISDKEVDA IMNRLQQVGD KPRPKTLFEP GEMVRVNDGP FADFNGVVEE
160 170 180
VDYEKSRLKV SVSIFGRATP VELDFSQVEK A
Length:181
Mass (Da):20,532
Last modified:January 23, 2007 - v2
Checksum:iB3AF94284CC17B04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30610 Genomic DNA Translation: AAA24622.1
U00006 Genomic DNA Translation: AAC43080.1
U00096 Genomic DNA Translation: AAC76956.1
AP009048 Genomic DNA Translation: BAE77338.1
PIRiB35139 TWECNG
RefSeqiNP_418409.1, NC_000913.3
WP_001287516.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76956; AAC76956; b3982
BAE77338; BAE77338; BAE77338
GeneIDi948485
KEGGiecj:JW3945
eco:b3982
PATRICifig|1411691.4.peg.2730

Similar proteinsi

Entry informationi

Entry nameiNUSG_ECOLI
AccessioniPrimary (citable) accession number: P0AFG0
Secondary accession number(s): P16921, Q2M8R8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 103 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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