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UniProtKB - P0AFG0 (NUSG_ECOLI)

Entry version 121 (07 Apr 2021)
Sequence version 2 (23 Jan 2007)
Previous versions | rss
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Protein

Transcription termination/antitermination protein NusG

Gene

nusG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of the processive rRNA transcription and antitermination complex (rrnTAC). The complex forms an RNA-chaperone ring around the RNA exit tunnel of RNA polymerase (RNAP). It supports rapid transcription and antitermination of rRNA operons, cotranscriptional rRNA folding, and annealing of distal rRNA regions to allow correct ribosome biogenesis (PubMed:32871103). Participates in transcription elongation, termination and antitermination. In the absence of Rho, increases the rate of transcription elongation by the RNAP, probably by partially suppressing pausing. In the presence of Rho, modulates most Rho-dependent termination events by interacting with the RNAP to render the complex more susceptible to the termination activity of Rho. May be required to overcome a kinetic limitation of Rho to function at certain terminators. Also involved in ribosomal RNA and phage lambda N-mediated transcriptional antitermination.UniRule annotation10 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processRibosome biogenesis, Transcription, Transcription antitermination, Transcription regulation, Transcription termination

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG10667-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Transcription termination/antitermination protein NusGUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nusGUniRule annotation
Ordered Locus Names:b3982, JW3945
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemoved1 Publication
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001139262 – 181Transcription termination/antitermination protein NusGAdd BLAST180

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0AFG0

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AFG0

PRoteomics IDEntifications database

More...PRIDEi		P0AFG0

2D gel databases

Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

More...SWISS-2DPAGEi		P0AFG0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

Interacts with the transcription termination factor Rho and with RNAP. One NusG monomer forms a stable complex with a Rho hexamer. Binds directly, but weakly, to the core enzyme of RNAP.

Also interacts with RpsJ (NusE). The rRNA transcription and antitermination complex (rrnTAC) consists of RNAP, NusA, NusB, NusE (rpsJ), NusG, SubB, ribosomal protein S4, DNA and precursor rRNA; S4 is more flexible than other subunits (PubMed:32871103).

UniRule annotation5 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4259510, 11 interactors
852779, 4 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-5674, Transcription elongation complex
CPX-5780, lambdaN-dependent processive transcription antitermination complex

Database of interacting proteins

More...DIPi		DIP-31860N

Protein interaction database and analysis system

More...IntActi		P0AFG0, 61 interactors

Molecular INTeraction database

More...MINTi		P0AFG0

STRING: functional protein association networks

More...STRINGi		511145.b3982

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1181
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		P0AFG0

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AFG0

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P0AFG0

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini130 – 161KOWUniRule annotationAdd BLAST32

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The N-terminal domain interacts with RNAP and the C-terminal domain binds either to Rho or to RpsJ (NusE). These domains are separated by a flexible linker.1 Publication

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the NusG family.UniRule annotation

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0250, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_067287_1_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AFG0

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AFG0

Family and domain databases

Database of protein disorder

More...DisProti		DP01496

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		2.30.30.30, 1 hit
3.30.70.940, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_00948, NusG, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR005824, KOW
IPR006645, NGN_dom
IPR036735, NGN_dom_sf
IPR043425, NusG-like
IPR014722, Rib_L2_dom2
IPR001062, Transcrpt_antiterm_NusG
IPR015869, Transcrpt_antiterm_NusG_bac_CS
IPR008991, Translation_prot_SH3-like_sf

The PANTHER Classification System

More...PANTHERi		PTHR30265, PTHR30265, 1 hit
PTHR30265:SF2, PTHR30265:SF2, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00467, KOW, 1 hit
PF02357, NusG, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00338, NUSGTNSCPFCT

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00739, KOW, 1 hit
SM00738, NGN, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF50104, SSF50104, 1 hit
SSF82679, SSF82679, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00922, nusG, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS01014, NUSG, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

P0AFG0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEAPKKRWY VVQAFSGFEG RVATSLREHI KLHNMEDLFG EVMVPTEEVV 
        60         70         80         90        100
EIRGGQRRKS ERKFFPGYVL VQMVMNDASW HLVRSVPRVM GFIGGTSDRP 
       110        120        130        140        150
APISDKEVDA IMNRLQQVGD KPRPKTLFEP GEMVRVNDGP FADFNGVVEE 
       160        170        180 
VDYEKSRLKV SVSIFGRATP VELDFSQVEK A
Length:181
Mass (Da):20,532
Last modified:January 23, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB3AF94284CC17B04
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M30610 Genomic DNA Translation: AAA24622.1
U00006 Genomic DNA Translation: AAC43080.1
U00096 Genomic DNA Translation: AAC76956.1
AP009048 Genomic DNA Translation: BAE77338.1

Protein sequence database of the Protein Information Resource

More...PIRi		B35139, TWECNG

NCBI Reference Sequences

More...RefSeqi		NP_418409.1, NC_000913.3
WP_001287516.1, NZ_STEB01000045.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76956; AAC76956; b3982
BAE77338; BAE77338; BAE77338

Database of genes from NCBI RefSeq genomes

More...GeneIDi		58390080
948485

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3945
eco:b3982

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.2730

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M30610 Genomic DNA Translation: AAA24622.1
U00006 Genomic DNA Translation: AAC43080.1
U00096 Genomic DNA Translation: AAC76956.1
AP009048 Genomic DNA Translation: BAE77338.1
PIRiB35139, TWECNG
RefSeqiNP_418409.1, NC_000913.3
WP_001287516.1, NZ_STEB01000045.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JVVNMR-A1-181[»]
2K06NMR-A1-123[»]
2KVQNMR-G123-181[»]
5MS0electron microscopy9.80F1-181[»]
6C6Uelectron microscopy3.70N1-181[»]
6DUQX-ray3.70M/N/O/P/Q/R/S/T/U/V/W/X125-181[»]
6TQNelectron microscopy3.80G1-181[»]
6TQOelectron microscopy4.00G1-181[»]
6XAVelectron microscopy7.70L1-181[»]
6XE0electron microscopy6.80V1-181[»]
BMRBiP0AFG0
SMRiP0AFG0
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4259510, 11 interactors
852779, 4 interactors
ComplexPortaliCPX-5674, Transcription elongation complex
CPX-5780, lambdaN-dependent processive transcription antitermination complex
DIPiDIP-31860N
IntActiP0AFG0, 61 interactors
MINTiP0AFG0
STRINGi511145.b3982

2D gel databases

SWISS-2DPAGEiP0AFG0

Proteomic databases

jPOSTiP0AFG0
PaxDbiP0AFG0
PRIDEiP0AFG0

Genome annotation databases

EnsemblBacteriaiAAC76956; AAC76956; b3982
BAE77338; BAE77338; BAE77338
GeneIDi58390080
948485
KEGGiecj:JW3945
eco:b3982
PATRICifig|1411691.4.peg.2730

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0661

Phylogenomic databases

eggNOGiCOG0250, Bacteria
HOGENOMiCLU_067287_1_0_6
InParanoidiP0AFG0
PhylomeDBiP0AFG0

Enzyme and pathway databases

BioCyciEcoCyc:EG10667-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AFG0

Protein Ontology

More...PROi		PR:P0AFG0

Family and domain databases

DisProtiDP01496
Gene3Di2.30.30.30, 1 hit
3.30.70.940, 1 hit
HAMAPiMF_00948, NusG, 1 hit
InterProiView protein in InterPro
IPR005824, KOW
IPR006645, NGN_dom
IPR036735, NGN_dom_sf
IPR043425, NusG-like
IPR014722, Rib_L2_dom2
IPR001062, Transcrpt_antiterm_NusG
IPR015869, Transcrpt_antiterm_NusG_bac_CS
IPR008991, Translation_prot_SH3-like_sf
PANTHERiPTHR30265, PTHR30265, 1 hit
PTHR30265:SF2, PTHR30265:SF2, 1 hit
PfamiView protein in Pfam
PF00467, KOW, 1 hit
PF02357, NusG, 1 hit
PRINTSiPR00338, NUSGTNSCPFCT
SMARTiView protein in SMART
SM00739, KOW, 1 hit
SM00738, NGN, 1 hit
SUPFAMiSSF50104, SSF50104, 1 hit
SSF82679, SSF82679, 1 hit
TIGRFAMsiTIGR00922, nusG, 1 hit
PROSITEiView protein in PROSITE
PS01014, NUSG, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNUSG_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AFG0
Secondary accession number(s): P16921, Q2M8R8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: April 7, 2021
This is version 121 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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