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UniProtKB - P0AFF4 (NUPG_ECOLI)
Protein
Nucleoside permease NupG
Gene
nupG
Organism
Escherichia coli (strain K12)
Status
Functioni
Broad-specificity transporter of purine and pyrimidine nucleosides. Driven by a proton motive force. Can transport uridine, adenosine, inosine, guanosine, thymidine and cytidine. Can also transport xanthosine, but with a very low affinity.
5 PublicationsMiscellaneous
Binding of nucleosides to NupG requires the presence of hydroxyl groups at each of the C-3' and C-5' positions of ribose.1 Publication
Activity regulationi
Inhibited by the protonophore uncouplers 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by valinomycin.1 Publication
Kineticsi
- KM=23.6 µM for uridine1 Publication
- KM=20.6 µM for adenosine1 Publication
- Vmax=67.2 nmol/min/mg enzyme with uridine as substrate1 Publication
- Vmax=56.8 nmol/min/mg enzyme with adenosine as substrate1 Publication
GO - Molecular functioni
- cytidine transmembrane transporter activity Source: EcoliWiki
- nucleoside:proton symporter activity Source: UniProtKB-UniRule
- pyrimidine nucleoside transmembrane transporter activity Source: EcoliWiki
- uridine transmembrane transporter activity Source: EcoliWiki
GO - Biological processi
- adenosine transport Source: EcoliWiki
- organic substance transport Source: GO_Central
- purine nucleoside transmembrane transport Source: EcoliWiki
- pyrimidine nucleoside transport Source: EcoliWiki
- uridine transport Source: EcoliWiki
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:NUPG-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.10.1, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Nucleoside permease NupGUniRule annotationCuratedAlternative name(s): Nucleoside-transport system protein NupGCurated |
Gene namesi | Name:nupG1 PublicationUniRule annotation Ordered Locus Names:b2964, JW2932 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation3 Publications; Multi-pass membrane protein UniRule annotation
Plasma Membrane
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | CytoplasmicCurated | 8 | |
Transmembranei | 9 – 29 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 30 – 39 | PeriplasmicCurated | 10 | |
Transmembranei | 40 – 60 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 61 – 69 | CytoplasmicCurated | 9 | |
Transmembranei | 70 – 90 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 91 – 95 | PeriplasmicCurated | 5 | |
Transmembranei | 96 – 116 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 117 – 136 | CytoplasmicCuratedAdd BLAST | 20 | |
Transmembranei | 137 – 157 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 158 – 161 | PeriplasmicCurated | 4 | |
Transmembranei | 162 – 182 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 183 – 209 | CytoplasmicCuratedAdd BLAST | 27 | |
Transmembranei | 210 – 230 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 231 – 254 | PeriplasmicCuratedAdd BLAST | 24 | |
Transmembranei | 255 – 275 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 276 – 282 | CytoplasmicCurated | 7 | |
Transmembranei | 283 – 303 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 304 | PeriplasmicCurated | 1 | |
Transmembranei | 305 – 325 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 326 – 348 | CytoplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 349 – 369 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 370 – 381 | PeriplasmicCuratedAdd BLAST | 12 | |
Transmembranei | 382 – 402 | HelicalUniRule annotationAdd BLAST | 21 | |
Topological domaini | 403 – 418 | Cytoplasmic1 PublicationAdd BLAST | 16 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058005 | 1 – 418 | Nucleoside permease NupGAdd BLAST | 418 |
Proteomic databases
jPOSTi | P0AFF4 |
PaxDbi | P0AFF4 |
PRIDEi | P0AFF4 |
Expressioni
Inductioni
Transcriptionally regulated by CytR and DeoR.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262362, 113 interactors |
IntActi | P0AFF4, 1 interactor |
STRINGi | 511145.b2964 |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family. [View classification]UniRule annotationCurated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2211, Bacteria |
HOGENOMi | CLU_013133_1_2_6 |
InParanoidi | P0AFF4 |
PhylomeDBi | P0AFF4 |
Family and domain databases
Gene3Di | 1.20.1250.20, 2 hits |
HAMAPi | MF_02049, NupG, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004740, Nuc_H_symport IPR033667, NupG |
Pfami | View protein in Pfam PF03825, Nuc_H_symport, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00889, 2A0110, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFF4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLKLQLKIL SFLQFCLWGS WLTTLGSYMF VTLKFDGASI GAVYSSLGIA
60 70 80 90 100
AVFMPALLGI VADKWLSAKW VYAICHTIGA ITLFMAAQVT TPEAMFLVIL
110 120 130 140 150
INSFAYMPTL GLINTISYYR LQNAGMDIVT DFPPIRIWGT IGFIMAMWVV
160 170 180 190 200
SLSGFELSHM QLYIGAALSA ILVLFTLTLP HIPVAKQQAN QSWTTLLGLD
210 220 230 240 250
AFALFKNKRM AIFFIFSMLL GAELQITNMF GNTFLHSFDK DPMFASSFIV
260 270 280 290 300
QHASIIMSIS QISETLFILT IPFFLSRYGI KNVMMISIVA WILRFALFAY
310 320 330 340 350
GDPTPFGTVL LVLSMIVYGC AFDFFNISGS VFVEKEVSPA IRASAQGMFL
360 370 380 390 400
MMTNGFGCIL GGIVSGKVVE MYTQNGITDW QTVWLIFAGY SVVLAFAFMA
410
MFKYKHVRVP TGTQTVSH
Sequence cautioni
The sequence AAA69132 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 3 | L → S AA sequence (PubMed:15513740).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06174 Genomic DNA Translation: CAA29541.1 U28377 Genomic DNA Translation: AAA69132.1 Different initiation. U00096 Genomic DNA Translation: AAC76001.2 AP009048 Genomic DNA Translation: BAE77027.1 |
RefSeqi | NP_417439.4, NC_000913.3 WP_001049791.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC76001; AAC76001; b2964 BAE77027; BAE77027; BAE77027 |
GeneIDi | 66673154 946282 |
KEGGi | ecj:JW2932 eco:b2964 |
PATRICi | fig|1411691.4.peg.3766 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06174 Genomic DNA Translation: CAA29541.1 U28377 Genomic DNA Translation: AAA69132.1 Different initiation. U00096 Genomic DNA Translation: AAC76001.2 AP009048 Genomic DNA Translation: BAE77027.1 |
RefSeqi | NP_417439.4, NC_000913.3 WP_001049791.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7DL9 | X-ray | 3.00 | A/B | 1-418 | [»] | |
7DLA | X-ray | 3.00 | A | 1-418 | [»] | |
SMRi | P0AFF4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262362, 113 interactors |
IntActi | P0AFF4, 1 interactor |
STRINGi | 511145.b2964 |
Protein family/group databases
TCDBi | 2.A.1.10.1, the major facilitator superfamily (mfs) |
Proteomic databases
jPOSTi | P0AFF4 |
PaxDbi | P0AFF4 |
PRIDEi | P0AFF4 |
Genome annotation databases
EnsemblBacteriai | AAC76001; AAC76001; b2964 BAE77027; BAE77027; BAE77027 |
GeneIDi | 66673154 946282 |
KEGGi | ecj:JW2932 eco:b2964 |
PATRICi | fig|1411691.4.peg.3766 |
Organism-specific databases
EchoBASEi | EB0658 |
Phylogenomic databases
eggNOGi | COG2211, Bacteria |
HOGENOMi | CLU_013133_1_2_6 |
InParanoidi | P0AFF4 |
PhylomeDBi | P0AFF4 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUPG-MONOMER |
Miscellaneous databases
PROi | PR:P0AFF4 |
Family and domain databases
Gene3Di | 1.20.1250.20, 2 hits |
HAMAPi | MF_02049, NupG, 1 hit |
InterProi | View protein in InterPro IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR004740, Nuc_H_symport IPR033667, NupG |
Pfami | View protein in Pfam PF03825, Nuc_H_symport, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00889, 2A0110, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NUPG_ECOLI | |
Accessioni | P0AFF4Primary (citable) accession number: P0AFF4 Secondary accession number(s): P09452, P76653, Q2M9M9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families