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UniProtKB - P0AFF4 (NUPG_ECOLI)

Entry version 115 (23 Feb 2022)
Sequence version 1 (20 Dec 2005)
Previous versions | rss
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Protein

Nucleoside permease NupG

Gene

nupG

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Broad-specificity transporter of purine and pyrimidine nucleosides. Driven by a proton motive force. Can transport uridine, adenosine, inosine, guanosine, thymidine and cytidine. Can also transport xanthosine, but with a very low affinity.

5 Publications

Miscellaneous

Binding of nucleosides to NupG requires the presence of hydroxyl groups at each of the C-3' and C-5' positions of ribose.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by the protonophore uncouplers 2,4-dinitrophenol and carbonyl cyanide m-chlorophenylhydrazone (CCCP), and by valinomycin.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=23.6 µM for uridine1 Publication
  2. KM=20.6 µM for adenosine1 Publication
  1. Vmax=67.2 nmol/min/mg enzyme with uridine as substrate1 Publication
  2. Vmax=56.8 nmol/min/mg enzyme with adenosine as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:NUPG-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.1.10.1, the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Nucleoside permease NupGUniRule annotationCurated
Alternative name(s):
Nucleoside-transport system protein NupGCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nupG1 PublicationUniRule annotation
Ordered Locus Names:b2964, JW2932
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 8CytoplasmicCurated8
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei9 – 29HelicalUniRule annotationAdd BLAST21
Topological domaini30 – 39PeriplasmicCurated10
Transmembranei40 – 60HelicalUniRule annotationAdd BLAST21
Topological domaini61 – 69CytoplasmicCurated9
Transmembranei70 – 90HelicalUniRule annotationAdd BLAST21
Topological domaini91 – 95PeriplasmicCurated5
Transmembranei96 – 116HelicalUniRule annotationAdd BLAST21
Topological domaini117 – 136CytoplasmicCuratedAdd BLAST20
Transmembranei137 – 157HelicalUniRule annotationAdd BLAST21
Topological domaini158 – 161PeriplasmicCurated4
Transmembranei162 – 182HelicalUniRule annotationAdd BLAST21
Topological domaini183 – 209CytoplasmicCuratedAdd BLAST27
Transmembranei210 – 230HelicalUniRule annotationAdd BLAST21
Topological domaini231 – 254PeriplasmicCuratedAdd BLAST24
Transmembranei255 – 275HelicalUniRule annotationAdd BLAST21
Topological domaini276 – 282CytoplasmicCurated7
Transmembranei283 – 303HelicalUniRule annotationAdd BLAST21
Topological domaini304PeriplasmicCurated1
Transmembranei305 – 325HelicalUniRule annotationAdd BLAST21
Topological domaini326 – 348CytoplasmicCuratedAdd BLAST23
Transmembranei349 – 369HelicalUniRule annotationAdd BLAST21
Topological domaini370 – 381PeriplasmicCuratedAdd BLAST12
Transmembranei382 – 402HelicalUniRule annotationAdd BLAST21
Topological domaini403 – 418Cytoplasmic1 PublicationAdd BLAST16

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000580051 – 418Nucleoside permease NupGAdd BLAST418

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0AFF4

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AFF4

PRoteomics IDEntifications database

More...PRIDEi		P0AFF4

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transcriptionally regulated by CytR and DeoR.1 Publication

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4262362, 113 interactors

Protein interaction database and analysis system

More...IntActi		P0AFF4, 1 interactor

STRING: functional protein association networks

More...STRINGi		511145.b2964

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AFF4

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the major facilitator superfamily. Nucleoside:H(+) symporter (NHS) (TC 2.A.1.10) family. [View classification]UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2211, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_013133_1_2_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AFF4

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AFF4

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1250.20, 2 hits

HAMAP database of protein families

More...HAMAPi		MF_02049, NupG, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
IPR004740, Nuc_H_symport
IPR033667, NupG

Pfam protein domain database

More...Pfami		View protein in Pfam
PF03825, Nuc_H_symport, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103473, SSF103473, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00889, 2A0110, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50850, MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AFF4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLKLQLKIL SFLQFCLWGS WLTTLGSYMF VTLKFDGASI GAVYSSLGIA 
        60         70         80         90        100
AVFMPALLGI VADKWLSAKW VYAICHTIGA ITLFMAAQVT TPEAMFLVIL 
       110        120        130        140        150
INSFAYMPTL GLINTISYYR LQNAGMDIVT DFPPIRIWGT IGFIMAMWVV 
       160        170        180        190        200
SLSGFELSHM QLYIGAALSA ILVLFTLTLP HIPVAKQQAN QSWTTLLGLD 
       210        220        230        240        250
AFALFKNKRM AIFFIFSMLL GAELQITNMF GNTFLHSFDK DPMFASSFIV 
       260        270        280        290        300
QHASIIMSIS QISETLFILT IPFFLSRYGI KNVMMISIVA WILRFALFAY 
       310        320        330        340        350
GDPTPFGTVL LVLSMIVYGC AFDFFNISGS VFVEKEVSPA IRASAQGMFL 
       360        370        380        390        400
MMTNGFGCIL GGIVSGKVVE MYTQNGITDW QTVWLIFAGY SVVLAFAFMA 
       410 
MFKYKHVRVP TGTQTVSH
Length:418
Mass (Da):46,389
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i01337ECEFC369266
GO

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAA69132 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti3L → S AA sequence (PubMed:15513740).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X06174 Genomic DNA Translation: CAA29541.1
U28377 Genomic DNA Translation: AAA69132.1 Different initiation.
U00096 Genomic DNA Translation: AAC76001.2
AP009048 Genomic DNA Translation: BAE77027.1

NCBI Reference Sequences

More...RefSeqi		NP_417439.4, NC_000913.3
WP_001049791.1, NZ_STEB01000001.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76001; AAC76001; b2964
BAE77027; BAE77027; BAE77027

Database of genes from NCBI RefSeq genomes

More...GeneIDi		66673154
946282

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2932
eco:b2964

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3766

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X06174 Genomic DNA Translation: CAA29541.1
U28377 Genomic DNA Translation: AAA69132.1 Different initiation.
U00096 Genomic DNA Translation: AAC76001.2
AP009048 Genomic DNA Translation: BAE77027.1
RefSeqiNP_417439.4, NC_000913.3
WP_001049791.1, NZ_STEB01000001.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
7DL9X-ray3.00A/B1-418[»]
7DLAX-ray3.00A1-418[»]
SMRiP0AFF4
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4262362, 113 interactors
IntActiP0AFF4, 1 interactor
STRINGi511145.b2964

Protein family/group databases

TCDBi2.A.1.10.1, the major facilitator superfamily (mfs)

Proteomic databases

jPOSTiP0AFF4
PaxDbiP0AFF4
PRIDEiP0AFF4

Genome annotation databases

EnsemblBacteriaiAAC76001; AAC76001; b2964
BAE77027; BAE77027; BAE77027
GeneIDi66673154
946282
KEGGiecj:JW2932
eco:b2964
PATRICifig|1411691.4.peg.3766

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0658

Phylogenomic databases

eggNOGiCOG2211, Bacteria
HOGENOMiCLU_013133_1_2_6
InParanoidiP0AFF4
PhylomeDBiP0AFF4

Enzyme and pathway databases

BioCyciEcoCyc:NUPG-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P0AFF4

Family and domain databases

Gene3Di1.20.1250.20, 2 hits
HAMAPiMF_02049, NupG, 1 hit
InterProiView protein in InterPro
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
IPR004740, Nuc_H_symport
IPR033667, NupG
PfamiView protein in Pfam
PF03825, Nuc_H_symport, 1 hit
SUPFAMiSSF103473, SSF103473, 1 hit
TIGRFAMsiTIGR00889, 2A0110, 1 hit
PROSITEiView protein in PROSITE
PS50850, MFS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNUPG_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AFF4
Secondary accession number(s): P09452, P76653, Q2M9M9
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: February 23, 2022
This is version 115 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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