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Protein

Nucleoside permease NupC

Gene

nupC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force. Transports cytidine, uridine, thymidine, adenosine and inosine. Can also transport xanthosine, but with a very low affinity.3 Publications

Miscellaneous

Binding of nucleosides to NupC requires the presence of hydroxyl groups at the C-3' position of ribose.1 Publication

GO - Molecular functioni

  • cytidine transmembrane transporter activity Source: EcoliWiki
  • nucleoside:proton symporter activity Source: EcoCyc
  • uridine transmembrane transporter activity Source: EcoliWiki

GO - Biological processi

  • nucleoside transmembrane transport Source: EcoliWiki
  • nucleoside transport Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:NUPC-MONOMER
MetaCyc:NUPC-MONOMER

Protein family/group databases

TCDBi2.A.41.1.1 the concentrative nucleoside transporter (cnt) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoside permease NupCCurated
Alternative name(s):
Nucleoside-transport system protein NupCCurated
Gene namesi
Name:nupC1 Publication
Synonyms:cru
Ordered Locus Names:b2393, JW2389
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11971 nupC

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 3CytoplasmicCurated3
Transmembranei4 – 24HelicalSequence analysisAdd BLAST21
Topological domaini25 – 36PeriplasmicCuratedAdd BLAST12
Transmembranei37 – 57HelicalSequence analysisAdd BLAST21
Topological domaini58 – 86CytoplasmicCuratedAdd BLAST29
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 168PeriplasmicCuratedAdd BLAST61
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 192CytoplasmicCurated3
Transmembranei193 – 213HelicalSequence analysisAdd BLAST21
Topological domaini214 – 250PeriplasmicCuratedAdd BLAST37
Transmembranei251 – 271HelicalSequence analysisAdd BLAST21
Topological domaini272 – 281CytoplasmicCurated10
Transmembranei282 – 302HelicalSequence analysisAdd BLAST21
Topological domaini303 – 341PeriplasmicCuratedAdd BLAST39
Transmembranei342 – 362HelicalSequence analysisAdd BLAST21
Topological domaini363 – 378CytoplasmicCuratedAdd BLAST16
Transmembranei379 – 399HelicalSequence analysisAdd BLAST21
Topological domaini400Periplasmic1 Publication1

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000704541 – 400Nucleoside permease NupCAdd BLAST400

Proteomic databases

PaxDbiP0AFF2
PRIDEiP0AFF2

Interactioni

Protein-protein interaction databases

BioGridi4259184, 18 interactors
STRINGi316385.ECDH10B_2557

Structurei

3D structure databases

ProteinModelPortaliP0AFF2
SMRiP0AFF2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D9N Bacteria
COG1972 LUCA
HOGENOMiHOG000267657
InParanoidiP0AFF2
KOiK11535
OMAiVYGDRIC
PhylomeDBiP0AFF2

Family and domain databases

InterProiView protein in InterPro
IPR008276 C_nuclsd_transpt
IPR018270 C_nuclsd_transpt_met_bac
IPR011657 CNT_C_dom
IPR002668 CNT_N_dom
IPR011642 Gate_dom
PANTHERiPTHR10590 PTHR10590, 1 hit
PfamiView protein in Pfam
PF07670 Gate, 1 hit
PF07662 Nucleos_tra2_C, 1 hit
PF01773 Nucleos_tra2_N, 1 hit
TIGRFAMsiTIGR00804 nupC, 1 hit

Sequencei

Sequence statusi: Complete.

P0AFF2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRVLHFVLA LAVVAILALL VSSDRKKIRI RYVIQLLVIE VLLAWFFLNS
60 70 80 90 100
DVGLGFVKGF SEMFEKLLGF ANEGTNFVFG SMNDQGLAFF FLKVLCPIVF
110 120 130 140 150
ISALIGILQH IRVLPVIIRA IGFLLSKVNG MGKLESFNAV SSLILGQSEN
160 170 180 190 200
FIAYKDILGK ISRNRMYTMA ATAMSTVSMS IVGAYMTMLE PKYVVAALVL
210 220 230 240 250
NMFSTFIVLS LINPYRVDAS EENIQMSNLH EGQSFFEMLG EYILAGFKVA
260 270 280 290 300
IIVAAMLIGF IALIAALNAL FATVTGWFGY SISFQGILGY IFYPIAWVMG
310 320 330 340 350
VPSSEALQVG SIMATKLVSN EFVAMMDLQK IASTLSPRAE GIISVFLVSF
360 370 380 390 400
ANFSSIGIIA GAVKGLNEEQ GNVVSRFGLK LVYGSTLVSV LSASIAALVL
Length:400
Mass (Da):43,476
Last modified:December 20, 2005 - v1
Checksum:i1EA1DE138C4351CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti82Missing in CAA52821 (PubMed:8022285).Curated1
Sequence conflicti88A → AE in CAA52821 (PubMed:8022285).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74825 Genomic DNA Translation: CAA52821.1
U00096 Genomic DNA Translation: AAC75452.1
AP009048 Genomic DNA Translation: BAA16263.1
PIRiF65013
RefSeqiNP_416894.1, NC_000913.3
WP_000376337.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75452; AAC75452; b2393
BAA16263; BAA16263; BAA16263
GeneIDi946895
KEGGiecj:JW2389
eco:b2393
PATRICifig|1411691.4.peg.4336

Similar proteinsi

Entry informationi

Entry nameiNUPC_ECOLI
AccessioniPrimary (citable) accession number: P0AFF2
Secondary accession number(s): P33031, P77236
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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