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UniProtKB - P0AFE8 (NUOM_ECOLI)
Protein
NADH-quinone oxidoreductase subunit M
Gene
nuoM
Organism
Escherichia coli (strain K12)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activityi
GO - Molecular functioni
- NADH dehydrogenase (ubiquinone) activity Source: InterPro
- ubiquinone binding Source: EcoCyc
GO - Biological processi
- aerobic respiration Source: EcoCyc
- ATP synthesis coupled electron transport Source: InterPro
- electron transport coupled proton transport Source: EcoCyc
- respiratory electron transport chain Source: ComplexPortal
Keywordsi
Molecular function | Translocase |
Ligand | NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOM-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit M (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit M NDH-1 subunit M NUO13 |
Gene namesi | Name:nuoM Ordered Locus Names:b2277, JW2272 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
- plasma membrane respiratory chain complex I Source: EcoCyc
Other locations
- membrane Source: ComplexPortal
- NADH dehydrogenase complex Source: EcoliWiki
- respiratory chain complex I Source: ComplexPortal
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 22 – 29 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 30 – 50 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 51 – 82 | PeriplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 83 – 103 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 104 – 121 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 122 – 142 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 143 – 173 | PeriplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 174 – 194 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 195 – 221 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 222 – 242 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 243 – 258 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 259 – 279 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 280 – 285 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 286 – 306 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 307 – 313 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 314 – 334 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 335 – 339 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 340 – 360 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 361 – 382 | PeriplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 383 – 403 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 404 – 424 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 425 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 426 – 446 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 447 – 464 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 465 – 485 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 486 – 509 | PeriplasmicSequence analysisAdd BLAST | 24 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118039 | 1 – 509 | NADH-quinone oxidoreductase subunit MAdd BLAST | 509 |
Keywords - PTMi
QuinoneProteomic databases
jPOSTi | P0AFE8 |
PaxDbi | P0AFE8 |
PRIDEi | P0AFE8 |
Interactioni
Subunit structurei
Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Protein-protein interaction databases
BioGRIDi | 4260887, 53 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-59255N |
IntActi | P0AFE8, 1 interactor |
STRINGi | 511145.b2277 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P0AFE8 |
SMRi | P0AFE8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I subunit 4 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1008, Bacteria |
HOGENOMi | CLU_007100_4_4_6 |
InParanoidi | P0AFE8 |
OMAi | ITRWGNQ |
PhylomeDBi | P0AFE8 |
Family and domain databases
InterProi | View protein in InterPro IPR010227, NADH_Q_OxRdtase_chainM/4 IPR003918, NADH_UbQ_OxRdtase IPR001750, ND/Mrp_mem |
PANTHERi | PTHR43507, PTHR43507, 1 hit |
Pfami | View protein in Pfam PF00361, Proton_antipo_M, 1 hit |
PRINTSi | PR01437, NUOXDRDTASE4 |
TIGRFAMsi | TIGR01972, NDH_I_M, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFE8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLPWLILIP FIGGFLCWQT ERFGVKVPRW IALITMGLTL ALSLQLWLQG
60 70 80 90 100
GYSLTQSAGI PQWQSEFDMP WIPRFGISIH LAIDGLSLLM VVLTGLLGVL
110 120 130 140 150
AVLCSWKEIE KYQGFFHLNL MWILGGVIGV FLAIDMFLFF FFWEMMLVPM
160 170 180 190 200
YFLIALWGHK ASDGKTRITA ATKFFIYTQA SGLVMLIAIL ALVFVHYNAT
210 220 230 240 250
GVWTFNYEEL LNTPMSSGVE YLLMLGFFIA FAVKMPVVPL HGWLPDAHSQ
260 270 280 290 300
APTAGSVDLA GILLKTAAYG LLRFSLPLFP NASAEFAPIA MWLGVIGIFY
310 320 330 340 350
GAWMAFAQTD IKRLIAYTSV SHMGFVLIAI YTGSQLAYQG AVIQMIAHGL
360 370 380 390 400
SAAGLFILCG QLYERIHTRD MRMMGGLWSK MKWLPALSLF FAVATLGMPG
410 420 430 440 450
TGNFVGEFMI LFGSFQVVPV ITVISTFGLV FASVYSLAML HRAYFGKAKS
460 470 480 490 500
QIASQELPGM SLRELFMILL LVVLLVLLGF YPQPILDTSH SAIGNIQQWF
VNSVTTTRP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 464 – 465 | EL → DV (PubMed:7690854).Curated | 2 | |
Sequence conflicti | 464 – 465 | EL → DV (PubMed:8366049).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48372.1 L19568 Genomic DNA Translation: AAA53583.1 U00096 Genomic DNA Translation: AAC75337.1 AP009048 Genomic DNA Translation: BAA16105.1 |
PIRi | C64999 |
RefSeqi | NP_416780.1, NC_000913.3 WP_000926441.1, NZ_STEB01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC75337; AAC75337; b2277 BAA16105; BAA16105; BAA16105 |
GeneIDi | 66673840 947731 |
KEGGi | ecj:JW2272 eco:b2277 |
PATRICi | fig|511145.12.peg.2370 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48372.1 L19568 Genomic DNA Translation: AAA53583.1 U00096 Genomic DNA Translation: AAC75337.1 AP009048 Genomic DNA Translation: BAA16105.1 |
PIRi | C64999 |
RefSeqi | NP_416780.1, NC_000913.3 WP_000926441.1, NZ_STEB01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
7NYH | electron microscopy | 3.60 | M | 1-509 | [»] | |
7NYR | electron microscopy | 3.30 | M | 1-509 | [»] | |
7NYU | electron microscopy | 3.80 | M | 1-509 | [»] | |
7NYV | electron microscopy | 3.70 | M | 1-509 | [»] | |
AlphaFoldDBi | P0AFE8 | |||||
SMRi | P0AFE8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260887, 53 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-59255N |
IntActi | P0AFE8, 1 interactor |
STRINGi | 511145.b2277 |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Proteomic databases
jPOSTi | P0AFE8 |
PaxDbi | P0AFE8 |
PRIDEi | P0AFE8 |
Protocols and materials databases
DNASUi | 947731 |
Genome annotation databases
EnsemblBacteriai | AAC75337; AAC75337; b2277 BAA16105; BAA16105; BAA16105 |
GeneIDi | 66673840 947731 |
KEGGi | ecj:JW2272 eco:b2277 |
PATRICi | fig|511145.12.peg.2370 |
Organism-specific databases
EchoBASEi | EB1722 |
Phylogenomic databases
eggNOGi | COG1008, Bacteria |
HOGENOMi | CLU_007100_4_4_6 |
InParanoidi | P0AFE8 |
OMAi | ITRWGNQ |
PhylomeDBi | P0AFE8 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOM-MONOMER |
Miscellaneous databases
PROi | PR:P0AFE8 |
Family and domain databases
InterProi | View protein in InterPro IPR010227, NADH_Q_OxRdtase_chainM/4 IPR003918, NADH_UbQ_OxRdtase IPR001750, ND/Mrp_mem |
PANTHERi | PTHR43507, PTHR43507, 1 hit |
Pfami | View protein in Pfam PF00361, Proton_antipo_M, 1 hit |
PRINTSi | PR01437, NUOXDRDTASE4 |
TIGRFAMsi | TIGR01972, NDH_I_M, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOM_ECOLI | |
Accessioni | P0AFE8Primary (citable) accession number: P0AFE8 Secondary accession number(s): P31978, P78248 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families