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UniProtKB - P0AFE8 (NUOM_ECOLI)

Entry version 112 (10 Feb 2021)
Sequence version 1 (20 Dec 2005)
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Protein

NADH-quinone oxidoreductase subunit M

Gene

nuoM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTranslocase
LigandNAD, Ubiquinone

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:NUOM-MONOMER
MetaCyc:NUOM-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
NADH-quinone oxidoreductase subunit M (EC:7.1.1.-)
Alternative name(s):
NADH dehydrogenase I subunit M
NDH-1 subunit M
NUO13
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:nuoM
Ordered Locus Names:b2277, JW2272
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini22 – 29CytoplasmicSequence analysis8
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Topological domaini51 – 82PeriplasmicSequence analysisAdd BLAST32
Transmembranei83 – 103HelicalSequence analysisAdd BLAST21
Topological domaini104 – 121CytoplasmicSequence analysisAdd BLAST18
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 173PeriplasmicSequence analysisAdd BLAST31
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 221CytoplasmicSequence analysisAdd BLAST27
Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
Topological domaini243 – 258PeriplasmicSequence analysisAdd BLAST16
Transmembranei259 – 279HelicalSequence analysisAdd BLAST21
Topological domaini280 – 285CytoplasmicSequence analysis6
Transmembranei286 – 306HelicalSequence analysisAdd BLAST21
Topological domaini307 – 313PeriplasmicSequence analysis7
Transmembranei314 – 334HelicalSequence analysisAdd BLAST21
Topological domaini335 – 339CytoplasmicSequence analysis5
Transmembranei340 – 360HelicalSequence analysisAdd BLAST21
Topological domaini361 – 382PeriplasmicSequence analysisAdd BLAST22
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Topological domaini425PeriplasmicSequence analysis1
Transmembranei426 – 446HelicalSequence analysisAdd BLAST21
Topological domaini447 – 464CytoplasmicSequence analysisAdd BLAST18
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21
Topological domaini486 – 509PeriplasmicSequence analysisAdd BLAST24

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001180391 – 509NADH-quinone oxidoreductase subunit MAdd BLAST509

Keywords - PTMi

Quinone

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0AFE8

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AFE8

PRoteomics IDEntifications database

More...PRIDEi		P0AFE8

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260887, 53 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-243, Respiratory chain complex I

Database of interacting proteins

More...DIPi		DIP-59255N

Protein interaction database and analysis system

More...IntActi		P0AFE8, 1 interactor

STRING: functional protein association networks

More...STRINGi		511145.b2277

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AFE8

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the complex I subunit 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG1008, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_007100_4_4_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AFE8

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AFE8

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR010227, NADH_Q_OxRdtase_chainM/4
IPR003918, NADH_UbQ_OxRdtase
IPR001750, ND/Mrp_mem

The PANTHER Classification System

More...PANTHERi		PTHR43507, PTHR43507, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00361, Proton_antipo_M, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR01437, NUOXDRDTASE4

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01972, NDH_I_M, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AFE8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLPWLILIP FIGGFLCWQT ERFGVKVPRW IALITMGLTL ALSLQLWLQG 
        60         70         80         90        100
GYSLTQSAGI PQWQSEFDMP WIPRFGISIH LAIDGLSLLM VVLTGLLGVL 
       110        120        130        140        150
AVLCSWKEIE KYQGFFHLNL MWILGGVIGV FLAIDMFLFF FFWEMMLVPM 
       160        170        180        190        200
YFLIALWGHK ASDGKTRITA ATKFFIYTQA SGLVMLIAIL ALVFVHYNAT 
       210        220        230        240        250
GVWTFNYEEL LNTPMSSGVE YLLMLGFFIA FAVKMPVVPL HGWLPDAHSQ 
       260        270        280        290        300
APTAGSVDLA GILLKTAAYG LLRFSLPLFP NASAEFAPIA MWLGVIGIFY 
       310        320        330        340        350
GAWMAFAQTD IKRLIAYTSV SHMGFVLIAI YTGSQLAYQG AVIQMIAHGL 
       360        370        380        390        400
SAAGLFILCG QLYERIHTRD MRMMGGLWSK MKWLPALSLF FAVATLGMPG 
       410        420        430        440        450
TGNFVGEFMI LFGSFQVVPV ITVISTFGLV FASVYSLAML HRAYFGKAKS 
       460        470        480        490        500
QIASQELPGM SLRELFMILL LVVLLVLLGF YPQPILDTSH SAIGNIQQWF 

VNSVTTTRP
Length:509
Mass (Da):56,525
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i3F8395683908EF97
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti464 – 465EL → DV (PubMed:7690854).Curated2
Sequence conflicti464 – 465EL → DV (PubMed:8366049).Curated2

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48372.1
L19568 Genomic DNA Translation: AAA53583.1
U00096 Genomic DNA Translation: AAC75337.1
AP009048 Genomic DNA Translation: BAA16105.1

Protein sequence database of the Protein Information Resource

More...PIRi		C64999

NCBI Reference Sequences

More...RefSeqi		NP_416780.1, NC_000913.3
WP_000926441.1, NZ_STEB01000008.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75337; AAC75337; b2277
BAA16105; BAA16105; BAA16105

Database of genes from NCBI RefSeq genomes

More...GeneIDi		57728013
947731

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2272
eco:b2277

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|511145.12.peg.2370

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68301 Genomic DNA Translation: CAA48372.1
L19568 Genomic DNA Translation: AAA53583.1
U00096 Genomic DNA Translation: AAC75337.1
AP009048 Genomic DNA Translation: BAA16105.1
PIRiC64999
RefSeqiNP_416780.1, NC_000913.3
WP_000926441.1, NZ_STEB01000008.1

3D structure databases

SMRiP0AFE8
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4260887, 53 interactors
ComplexPortaliCPX-243, Respiratory chain complex I
DIPiDIP-59255N
IntActiP0AFE8, 1 interactor
STRINGi511145.b2277

Protein family/group databases

TCDBi3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Proteomic databases

jPOSTiP0AFE8
PaxDbiP0AFE8
PRIDEiP0AFE8

Protocols and materials databases

The DNASU plasmid repository

More...DNASUi		947731

Genome annotation databases

EnsemblBacteriaiAAC75337; AAC75337; b2277
BAA16105; BAA16105; BAA16105
GeneIDi57728013
947731
KEGGiecj:JW2272
eco:b2277
PATRICifig|511145.12.peg.2370

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB1722

Phylogenomic databases

eggNOGiCOG1008, Bacteria
HOGENOMiCLU_007100_4_4_6
InParanoidiP0AFE8
PhylomeDBiP0AFE8

Enzyme and pathway databases

BioCyciEcoCyc:NUOM-MONOMER
MetaCyc:NUOM-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P0AFE8

Family and domain databases

InterProiView protein in InterPro
IPR010227, NADH_Q_OxRdtase_chainM/4
IPR003918, NADH_UbQ_OxRdtase
IPR001750, ND/Mrp_mem
PANTHERiPTHR43507, PTHR43507, 1 hit
PfamiView protein in Pfam
PF00361, Proton_antipo_M, 1 hit
PRINTSiPR01437, NUOXDRDTASE4
TIGRFAMsiTIGR01972, NDH_I_M, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiNUOM_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AFE8
Secondary accession number(s): P31978, P78248
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: February 10, 2021
This is version 112 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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