UniProtKB - P0AFD6 (NUOI_ECOLI)
Protein
NADH-quinone oxidoreductase subunit I
Gene
nuoI
Organism
Escherichia coli (strain K12)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activityi
Cofactori
[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 60 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 63 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 66 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 70 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 99 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 102 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 105 | Iron-sulfur 2 (4Fe-4S)By similarity | 1 | |
Metal bindingi | 109 | Iron-sulfur 1 (4Fe-4S)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- iron ion binding Source: UniProtKB-UniRule
- NADH dehydrogenase (quinone) activity Source: UniProtKB-UniRule
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- aerobic respiration Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOI-MONOMER MetaCyc:NUOI-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit I (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit I NDH-1 subunit I NUO9 |
Gene namesi | Name:nuoI Ordered Locus Names:b2281, JW2276 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane Curated; Peripheral membrane protein Curated
Plasma Membrane
- plasma membrane Source: EcoCyc
- plasma membrane respiratory chain complex I Source: EcoCyc
Other locations
- NADH dehydrogenase complex Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118722 | 1 – 180 | NADH-quinone oxidoreductase subunit IAdd BLAST | 180 |
Keywords - PTMi
QuinoneProteomic databases
jPOSTi | P0AFD6 |
PaxDbi | P0AFD6 |
PRIDEi | P0AFD6 |
Interactioni
Subunit structurei
NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 4260510, 80 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-35918N |
IntActi | P0AFD6, 8 interactors |
STRINGi | 511145.b2281 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 80 | 4Fe-4S ferredoxin-type 1Add BLAST | 31 | |
Domaini | 90 – 119 | 4Fe-4S ferredoxin-type 2Add BLAST | 30 |
Sequence similaritiesi
Belongs to the complex I 23 kDa subunit family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG1143, Bacteria |
HOGENOMi | CLU_067218_4_3_6 |
InParanoidi | P0AFD6 |
PhylomeDBi | P0AFD6 |
Family and domain databases
HAMAPi | MF_01351, NDH1_NuoI, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR010226, NADH_quinone_OxRdtase_chainI |
PANTHERi | PTHR10849, PTHR10849, 1 hit |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit |
TIGRFAMsi | TIGR01971, NuoI, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
i Sequence
Sequence statusi: Complete.
P0AFD6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLKELLVGF GTQVRSIWMI GLHAFAKRET RMYPEEPVYL PPRYRGRIVL
60 70 80 90 100
TRDPDGEERC VACNLCAVAC PVGCISLQKA ETKDGRWYPE FFRINFSRCI
110 120 130 140 150
FCGLCEEACP TTAIQLTPDF EMGEYKRQDL VYEKEDLLIS GPGKYPEYNF
160 170 180
YRMAGMAIDG KDKGEAENEA KPIDVKSLLP
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 – 29 | AFAKRE → GSPNQ in CAA48368 (PubMed:7690854).Curated | 6 | |
Sequence conflicti | 165 – 175 | EAENEAKPIDV → DRRTKPSLSTF in CAA48368 (PubMed:7690854).CuratedAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48368.1 U00096 Genomic DNA Translation: AAC75341.1 AP009048 Genomic DNA Translation: BAA16109.2 |
PIRi | G64999 |
RefSeqi | NP_416784.1, NC_000913.3 WP_000172749.1, NZ_STEB01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC75341; AAC75341; b2281 BAA16109; BAA16109; BAA16109 |
GeneIDi | 58462906 946757 |
KEGGi | ecj:JW2276 eco:b2281 |
PATRICi | fig|511145.12.peg.2374 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48368.1 U00096 Genomic DNA Translation: AAC75341.1 AP009048 Genomic DNA Translation: BAA16109.2 |
PIRi | G64999 |
RefSeqi | NP_416784.1, NC_000913.3 WP_000172749.1, NZ_STEB01000008.1 |
3D structure databases
SMRi | P0AFD6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260510, 80 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
DIPi | DIP-35918N |
IntActi | P0AFD6, 8 interactors |
STRINGi | 511145.b2281 |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Proteomic databases
jPOSTi | P0AFD6 |
PaxDbi | P0AFD6 |
PRIDEi | P0AFD6 |
Genome annotation databases
EnsemblBacteriai | AAC75341; AAC75341; b2281 BAA16109; BAA16109; BAA16109 |
GeneIDi | 58462906 946757 |
KEGGi | ecj:JW2276 eco:b2281 |
PATRICi | fig|511145.12.peg.2374 |
Organism-specific databases
EchoBASEi | EB2013 |
Phylogenomic databases
eggNOGi | COG1143, Bacteria |
HOGENOMi | CLU_067218_4_3_6 |
InParanoidi | P0AFD6 |
PhylomeDBi | P0AFD6 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOI-MONOMER MetaCyc:NUOI-MONOMER |
Miscellaneous databases
PROi | PR:P0AFD6 |
Family and domain databases
HAMAPi | MF_01351, NDH1_NuoI, 1 hit |
InterProi | View protein in InterPro IPR017896, 4Fe4S_Fe-S-bd IPR017900, 4Fe4S_Fe_S_CS IPR010226, NADH_quinone_OxRdtase_chainI |
PANTHERi | PTHR10849, PTHR10849, 1 hit |
Pfami | View protein in Pfam PF12838, Fer4_7, 1 hit |
TIGRFAMsi | TIGR01971, NuoI, 1 hit |
PROSITEi | View protein in PROSITE PS00198, 4FE4S_FER_1, 2 hits PS51379, 4FE4S_FER_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOI_ECOLI | |
Accessioni | P0AFD6Primary (citable) accession number: P0AFD6 Secondary accession number(s): P33604, P76488, P78183 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families