UniProtKB - P0AFD4 (NUOH_ECOLI)
Protein
NADH-quinone oxidoreductase subunit H
Gene
nuoH
Organism
Escherichia coli (strain K12)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone.
Catalytic activityi
GO - Molecular functioni
- oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor Source: UniProtKB-UniRule
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- aerobic respiration Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Ligand | NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOH-MONOMER MetaCyc:NUOH-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit H (EC:7.1.1.-)Alternative name(s): NADH dehydrogenase I subunit H NDH-1 subunit H NUO8 |
Gene namesi | Name:nuoH Ordered Locus Names:b2282, JW2277 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
- plasma membrane respiratory chain complex I Source: EcoCyc
Other locations
- NADH dehydrogenase complex Source: EcoliWiki
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 11 – 31 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 81 – 101 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 154 – 174 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 186 – 206 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 237 – 257 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 265 – 285 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 304 – 324 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000117523 | 1 – 325 | NADH-quinone oxidoreductase subunit HAdd BLAST | 325 |
Keywords - PTMi
QuinoneProteomic databases
jPOSTi | P0AFD4 |
PaxDbi | P0AFD4 |
PRIDEi | P0AFD4 |
Interactioni
Subunit structurei
NDH-1 is composed of 13 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex.
Protein-protein interaction databases
BioGRIDi | 4260511, 40 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
IntActi | P0AFD4, 1 interactor |
STRINGi | 511145.b2282 |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I subunit 1 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1005, Bacteria |
HOGENOMi | CLU_015134_0_1_6 |
InParanoidi | P0AFD4 |
PhylomeDBi | P0AFD4 |
Family and domain databases
HAMAPi | MF_01350, NDH1_NuoH, 1 hit |
InterProi | View protein in InterPro IPR001694, NADH_UbQ_OxRdtase_su1/FPO IPR018086, NADH_UbQ_OxRdtase_su1_CS |
PANTHERi | PTHR11432, PTHR11432, 1 hit |
Pfami | View protein in Pfam PF00146, NADHdh, 1 hit |
PROSITEi | View protein in PROSITE PS00667, COMPLEX1_ND1_1, 1 hit PS00668, COMPLEX1_ND1_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFD4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSWISPELIE ILLTILKAVV ILLVVVTCGA FMSFGERRLL GLFQNRYGPN
60 70 80 90 100
RVGWGGSLQL VADMIKMFFK EDWIPKFSDR VIFTLAPMIA FTSLLLAFAI
110 120 130 140 150
VPVSPGWVVA DLNIGILFFL MMAGLAVYAV LFAGWSSNNK YSLLGAMRAS
160 170 180 190 200
AQTLSYEVFL GLSLMGVVAQ AGSFNMTDIV NSQAHVWNVI PQFFGFITFA
210 220 230 240 250
IAGVAVCHRH PFDQPEAEQE LADGYHIEYS GMKFGLFFVG EYIGIVTISA
260 270 280 290 300
LMVTLFFGGW QGPLLPPFIW FALKTAFFMM MFILIRASLP RPRYDQVMSF
310 320
GWKICLPLTL INLLVTAAVI LWQAQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 35 – 36 | GE → AK in CAA48367 (PubMed:7690854).Curated | 2 | |
Sequence conflicti | 53 | G → A in CAA48367 (PubMed:7690854).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48367.1 U00096 Genomic DNA Translation: AAC75342.1 AP009048 Genomic DNA Translation: BAA16110.1 |
PIRi | H64999 |
RefSeqi | NP_416785.1, NC_000913.3 WP_000118507.1, NZ_STEB01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC75342; AAC75342; b2282 BAA16110; BAA16110; BAA16110 |
GeneIDi | 58462907 946761 |
KEGGi | ecj:JW2277 eco:b2282 |
PATRICi | fig|1411691.4.peg.4454 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48367.1 U00096 Genomic DNA Translation: AAC75342.1 AP009048 Genomic DNA Translation: BAA16110.1 |
PIRi | H64999 |
RefSeqi | NP_416785.1, NC_000913.3 WP_000118507.1, NZ_STEB01000008.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260511, 40 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
IntActi | P0AFD4, 1 interactor |
STRINGi | 511145.b2282 |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Proteomic databases
jPOSTi | P0AFD4 |
PaxDbi | P0AFD4 |
PRIDEi | P0AFD4 |
Protocols and materials databases
DNASUi | 946761 |
Genome annotation databases
EnsemblBacteriai | AAC75342; AAC75342; b2282 BAA16110; BAA16110; BAA16110 |
GeneIDi | 58462907 946761 |
KEGGi | ecj:JW2277 eco:b2282 |
PATRICi | fig|1411691.4.peg.4454 |
Organism-specific databases
EchoBASEi | EB2012 |
Phylogenomic databases
eggNOGi | COG1005, Bacteria |
HOGENOMi | CLU_015134_0_1_6 |
InParanoidi | P0AFD4 |
PhylomeDBi | P0AFD4 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOH-MONOMER MetaCyc:NUOH-MONOMER |
Miscellaneous databases
PROi | PR:P0AFD4 |
Family and domain databases
HAMAPi | MF_01350, NDH1_NuoH, 1 hit |
InterProi | View protein in InterPro IPR001694, NADH_UbQ_OxRdtase_su1/FPO IPR018086, NADH_UbQ_OxRdtase_su1_CS |
PANTHERi | PTHR11432, PTHR11432, 1 hit |
Pfami | View protein in Pfam PF00146, NADHdh, 1 hit |
PROSITEi | View protein in PROSITE PS00667, COMPLEX1_ND1_1, 1 hit PS00668, COMPLEX1_ND1_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOH_ECOLI | |
Accessioni | P0AFD4Primary (citable) accession number: P0AFD4 Secondary accession number(s): P33603, P78307 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families