UniProtKB - P0AFC7 (NUOB_ECOLI)
Protein
NADH-quinone oxidoreductase subunit B
Gene
nuoB
Organism
Escherichia coli (strain K12)
Status
Functioni
NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
Catalytic activityi
Cofactori
[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 63 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 64 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 129 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 | |
Metal bindingi | 158 | Iron-sulfur (4Fe-4S)UniRule annotation | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: EcoCyc
- iron ion binding Source: UniProtKB-UniRule
- NADH dehydrogenase (ubiquinone) activity Source: EcoCyc
- quinone binding Source: UniProtKB-KW
GO - Biological processi
- aerobic respiration Source: EcoCyc
- electron transport coupled proton transport Source: EcoCyc
Keywordsi
Molecular function | Translocase |
Biological process | Transport |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOB-MONOMER MetaCyc:NUOB-MONOMER |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
Names & Taxonomyi
Protein namesi | Recommended name: NADH-quinone oxidoreductase subunit BUniRule annotation (EC:7.1.1.-UniRule annotation)Alternative name(s): NADH dehydrogenase I subunit BUniRule annotation NDH-1 subunit BUniRule annotation NUO2 |
Gene namesi | Name:nuoBUniRule annotation Ordered Locus Names:b2287, JW5875 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- membrane Source: UniProtKB
- NADH dehydrogenase complex Source: EcoliWiki
- plasma membrane Source: EcoliWiki
- plasma membrane respiratory chain complex I Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000118765 | 1 – 220 | NADH-quinone oxidoreductase subunit BAdd BLAST | 220 |
Keywords - PTMi
QuinoneProteomic databases
jPOSTi | P0AFC7 |
PaxDbi | P0AFC7 |
PRIDEi | P0AFC7 |
2D gel databases
SWISS-2DPAGEi | P0AFC7 |
Interactioni
Subunit structurei
NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
Protein-protein interaction databases
BioGRIDi | 4262975, 59 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
IntActi | P0AFC7, 1 interactor |
STRINGi | 511145.b2287 |
Family & Domainsi
Sequence similaritiesi
Belongs to the complex I 20 kDa subunit family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0377, Bacteria |
HOGENOMi | CLU_055737_7_3_6 |
InParanoidi | P0AFC7 |
PhylomeDBi | P0AFC7 |
Family and domain databases
HAMAPi | MF_01356, NDH1_NuoB, 1 hit |
InterProi | View protein in InterPro IPR006137, NADH_UbQ_OxRdtase-like_20kDa IPR006138, NADH_UQ_OxRdtase_20Kd_su |
Pfami | View protein in Pfam PF01058, Oxidored_q6, 1 hit |
TIGRFAMsi | TIGR01957, nuoB_fam, 1 hit |
PROSITEi | View protein in PROSITE PS01150, COMPLEX1_20K, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFC7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDYTLTRIDP NGENDRYPLQ KQEIVTDPLE QEVNKNVFMG KLNDMVNWGR
60 70 80 90 100
KNSIWPYNFG LSCCYVEMVT SFTAVHDVAR FGAEVLRASP RQADLMVVAG
110 120 130 140 150
TCFTKMAPVI QRLYDQMLEP KWVISMGACA NSGGMYDIYS VVQGVDKFIP
160 170 180 190 200
VDVYIPGCPP RPEAYMQALM LLQESIGKER RPLSWVVGDQ GVYRANMQSE
210 220
RERKRGERIA VTNLRTPDEI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 71 | S → L in CAA48361 (PubMed:7690854).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48361.1 U00096 Genomic DNA Translation: AAC75347.1 AP009048 Genomic DNA Translation: BAA16121.2 |
PIRi | E65000 |
RefSeqi | NP_416790.1, NC_000913.3 WP_000386733.1, NZ_STEB01000008.1 WP_046608242.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75347; AAC75347; b2287 BAA16121; BAA16121; BAA16121 |
GeneIDi | 52077603 946738 |
KEGGi | ecj:JW5875 eco:b2287 |
PATRICi | fig|1411691.4.peg.4449 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X68301 Genomic DNA Translation: CAA48361.1 U00096 Genomic DNA Translation: AAC75347.1 AP009048 Genomic DNA Translation: BAA16121.2 |
PIRi | E65000 |
RefSeqi | NP_416790.1, NC_000913.3 WP_000386733.1, NZ_STEB01000008.1 WP_046608242.1, NZ_LN832404.1 |
3D structure databases
SMRi | P0AFC7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262975, 59 interactors |
ComplexPortali | CPX-243, Respiratory chain complex I |
IntActi | P0AFC7, 1 interactor |
STRINGi | 511145.b2287 |
Protein family/group databases
TCDBi | 3.D.1.1.1, the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family |
2D gel databases
SWISS-2DPAGEi | P0AFC7 |
Proteomic databases
jPOSTi | P0AFC7 |
PaxDbi | P0AFC7 |
PRIDEi | P0AFC7 |
Genome annotation databases
EnsemblBacteriai | AAC75347; AAC75347; b2287 BAA16121; BAA16121; BAA16121 |
GeneIDi | 52077603 946738 |
KEGGi | ecj:JW5875 eco:b2287 |
PATRICi | fig|1411691.4.peg.4449 |
Organism-specific databases
EchoBASEi | EB2008 |
Phylogenomic databases
eggNOGi | COG0377, Bacteria |
HOGENOMi | CLU_055737_7_3_6 |
InParanoidi | P0AFC7 |
PhylomeDBi | P0AFC7 |
Enzyme and pathway databases
BioCyci | EcoCyc:NUOB-MONOMER MetaCyc:NUOB-MONOMER |
Miscellaneous databases
PROi | PR:P0AFC7 |
Family and domain databases
HAMAPi | MF_01356, NDH1_NuoB, 1 hit |
InterProi | View protein in InterPro IPR006137, NADH_UbQ_OxRdtase-like_20kDa IPR006138, NADH_UQ_OxRdtase_20Kd_su |
Pfami | View protein in Pfam PF01058, Oxidored_q6, 1 hit |
TIGRFAMsi | TIGR01957, nuoB_fam, 1 hit |
PROSITEi | View protein in PROSITE PS01150, COMPLEX1_20K, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUOB_ECOLI | |
Accessioni | P0AFC7Primary (citable) accession number: P0AFC7 Secondary accession number(s): P33598 P78187 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families