UniProtKB - P0AFC0 (NUDB_ECOLI)
Protein
Dihydroneopterin triphosphate diphosphatase
Gene
nudB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of dihydroneopterin triphosphate to dihydroneopterin monophosphate and pyrophosphate. Required for efficient folate biosynthesis. Can also hydrolyze nucleoside triphosphates with a preference for dATP.2 Publications
Catalytic activityi
- 7,8-dihydroneopterin 3'-triphosphate + H2O = 7,8-dihydroneopterin 3'-phosphate + diphosphate + H+1 PublicationEC:3.6.1.671 Publication
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
Activity regulationi
Subject to product inhibition by pyrophosphate.1 Publication
Kineticsi
- KM=0.27 mM for dihydroneopterin triphosphate2 Publications
- KM=0.79 mM for dATP2 Publications
pH dependencei
Optimum pH is 8.5-9.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 7 | Substrate | 1 | |
Binding sitei | 29 | Substrate | 1 | |
Binding sitei | 40 | Substrate | 1 | |
Metal bindingi | 56 | Magnesium | 1 | |
Metal bindingi | 60 | Magnesium | 1 | |
Metal bindingi | 117 | Magnesium | 1 | |
Binding sitei | 135 | SubstrateSequence analysis | 1 |
GO - Molecular functioni
- dATP pyrophosphohydrolase activity Source: EcoCyc
- dihydroneopterin triphosphate pyrophosphohydrolase activity Source: EcoCyc
- magnesium ion binding Source: EcoCyc
- metal ion binding Source: EcoCyc
GO - Biological processi
- folic acid biosynthetic process Source: EcoCyc
- tetrahydrofolate biosynthetic process Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | Folate biosynthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:H2NEOPTERINP3PYROPHOSPHOHYDRO-MONOMER MetaCyc:H2NEOPTERINP3PYROPHOSPHOHYDRO-MONOMER |
BRENDAi | 3.6.1.B15, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroneopterin triphosphate diphosphatase (EC:3.6.1.671 Publication)Alternative name(s): Dihydroneopterin triphosphate pyrophosphatase dATP pyrophosphohydrolase |
Gene namesi | Name:nudB Synonyms:ntpA Ordered Locus Names:b1865, JW1854 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056952 | 1 – 150 | Dihydroneopterin triphosphate diphosphataseAdd BLAST | 150 |
Proteomic databases
PaxDbi | P0AFC0 |
PRIDEi | P0AFC0 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260348, 315 interactors |
DIPi | DIP-10372N |
IntActi | P0AFC0, 7 interactors |
STRINGi | 511145.b1865 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFC0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFC0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 146 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 142 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 81 – 84 | Substrate bindingSequence analysis | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 41 – 62 | Nudix boxAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the Nudix hydrolase family.Curated
Phylogenomic databases
eggNOGi | COG0494, Bacteria |
HOGENOMi | CLU_128620_0_0_6 |
InParanoidi | P0AFC0 |
Family and domain databases
InterProi | View protein in InterPro IPR003564, DHNTPase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01404, NPPPHYDRLASE |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFC0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKDKVYKRPV SILVVIYAQD TKRVLMLQRR DDPDFWQSVT GSVEEGETAP
60 70 80 90 100
QAAMREVKEE VTIDVVAEQL TLIDCQRTVE FEIFSHLRHR YAPGVTRNTE
110 120 130 140 150
SWFCLALPHE RQIVFTEHLA YKWLDAPAAA ALTKSWSNRQ AIEQFVINAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59551 Genomic DNA Translation: CAA42124.1 D10165 Genomic DNA Translation: BAA01029.1 U00096 Genomic DNA Translation: AAC74935.1 AP009048 Genomic DNA Translation: BAA15676.1 |
PIRi | B38113 |
RefSeqi | NP_416379.1, NC_000913.3 WP_001300367.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74935; AAC74935; b1865 BAA15676; BAA15676; BAA15676 |
GeneIDi | 58389836 946383 |
KEGGi | ecj:JW1854 eco:b1865 |
PATRICi | fig|511145.12.peg.1944 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X59551 Genomic DNA Translation: CAA42124.1 D10165 Genomic DNA Translation: BAA01029.1 U00096 Genomic DNA Translation: AAC74935.1 AP009048 Genomic DNA Translation: BAA15676.1 |
PIRi | B38113 |
RefSeqi | NP_416379.1, NC_000913.3 WP_001300367.1, NZ_SSZK01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2O1C | X-ray | 1.80 | A/B/C/D | 1-150 | [»] | |
2O5W | X-ray | 2.60 | A/B/C/D | 1-150 | [»] | |
SMRi | P0AFC0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260348, 315 interactors |
DIPi | DIP-10372N |
IntActi | P0AFC0, 7 interactors |
STRINGi | 511145.b1865 |
Proteomic databases
PaxDbi | P0AFC0 |
PRIDEi | P0AFC0 |
Genome annotation databases
EnsemblBacteriai | AAC74935; AAC74935; b1865 BAA15676; BAA15676; BAA15676 |
GeneIDi | 58389836 946383 |
KEGGi | ecj:JW1854 eco:b1865 |
PATRICi | fig|511145.12.peg.1944 |
Organism-specific databases
EchoBASEi | EB1128 |
Phylogenomic databases
eggNOGi | COG0494, Bacteria |
HOGENOMi | CLU_128620_0_0_6 |
InParanoidi | P0AFC0 |
Enzyme and pathway databases
BioCyci | EcoCyc:H2NEOPTERINP3PYROPHOSPHOHYDRO-MONOMER MetaCyc:H2NEOPTERINP3PYROPHOSPHOHYDRO-MONOMER |
BRENDAi | 3.6.1.B15, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AFC0 |
PROi | PR:P0AFC0 |
Family and domain databases
InterProi | View protein in InterPro IPR003564, DHNTPase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR01404, NPPPHYDRLASE |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUDB_ECOLI | |
Accessioni | P0AFC0Primary (citable) accession number: P0AFC0 Secondary accession number(s): P24236 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families