UniProtKB - P0AFB8 (NTRC_ECOLI)
Protein
DNA-binding transcriptional regulator NtrC
Gene
glnG
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Phosphorylated NtrC binds directly to DNA and stimulates the formation of open promoter-sigma54-RNA polymerase complexes (PubMed:2874557, PubMed:3304660, PubMed:2574599, PubMed:1350679). Activates transcription of many genes and operons whose products minimize the slowing of growth under nitrogen-limiting conditions, including genes coding for glutamine synthetase (glnA), transporters, amino acid permeases and catabolic enzymes (PubMed:11121068).5 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 168 – 175 | ATPPROSITE-ProRule annotation | 8 | |
Nucleotide bindingi | 231 – 240 | ATPPROSITE-ProRule annotation | 10 | |
DNA bindingi | 445 – 464 | H-T-H motifBy similarityAdd BLAST | 20 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphorelay response regulator activity Source: InterPro
- sequence-specific DNA binding Source: InterPro
- transcription factor binding Source: InterPro
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
- regulation of nitrogen utilization Source: InterPro
- regulation of transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Activator, DNA-binding, Repressor |
Biological process | Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-NRI |
Names & Taxonomyi
Protein namesi | Recommended name: DNA-binding transcriptional regulator NtrCCuratedAlternative name(s): Nitrogen regulation protein NR(I)Curated Nitrogen regulator I1 Publication Short name: NRI1 Publication |
Gene namesi | Name:glnG1 Publication Synonyms:glnT, ntrC1 Publication Ordered Locus Names:b3868, JW3839 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm Curated
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000081165 | 1 – 469 | DNA-binding transcriptional regulator NtrCAdd BLAST | 469 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 54 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Phosphorylated and dephosphorylated by NtrB (PubMed:2874557, PubMed:2574599). Phosphorylation induces strong cooperative binding to DNA (PubMed:1350679).3 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AFB8 |
PaxDbi | P0AFB8 |
PRIDEi | P0AFB8 |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsGO - Molecular functioni
- transcription factor binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4262628, 38 interactors 852658, 2 interactors |
DIPi | DIP-9781N |
IntActi | P0AFB8, 6 interactors |
STRINGi | 511145.b3868 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 119 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 115 | |
Domaini | 140 – 369 | Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST | 230 |
Domaini
Contains an N-terminal receiver domain, a central output domain, which hydrolyzes ATP, interacts with RNA polymerase and is required for phosphorylation-dependent oligomerization, and a C-terminal domain, which is involved in DNA binding, dimerization and phosphorylation-independent oligomerization.1 Publication
Phylogenomic databases
eggNOGi | COG2204, Bacteria |
HOGENOMi | CLU_000445_0_1_6 |
InParanoidi | P0AFB8 |
PhylomeDBi | P0AFB8 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011006, CheY-like_superfamily IPR009057, Homeobox-like_sf IPR002197, HTH_Fis IPR027417, P-loop_NTPase IPR001789, Sig_transdc_resp-reg_receiver IPR002078, Sigma_54_int IPR025662, Sigma_54_int_dom_ATP-bd_1 IPR025943, Sigma_54_int_dom_ATP-bd_2 IPR025944, Sigma_54_int_dom_CS IPR010114, Transcript_reg_NtrC |
Pfami | View protein in Pfam PF02954, HTH_8, 1 hit PF00072, Response_reg, 1 hit PF00158, Sigma54_activat, 1 hit |
PRINTSi | PR01590, HTHFIS |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF52172, SSF52172, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01818, ntrC, 1 hit |
PROSITEi | View protein in PROSITE PS50110, RESPONSE_REGULATORY, 1 hit PS00675, SIGMA54_INTERACT_1, 1 hit PS00676, SIGMA54_INTERACT_2, 1 hit PS00688, SIGMA54_INTERACT_3, 1 hit PS50045, SIGMA54_INTERACT_4, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFB8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGAEVL EALASKTPDV
60 70 80 90 100
LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSDLDAA VSAYQQGAFD
110 120 130 140 150
YLPKPFDIDE AVALVERAIS HYQEQQQPRN VQLNGPTTDI IGEAPAMQDV
160 170 180 190 200
FRIIGRLSRS SISVLINGES GTGKELVAHA LHRHSPRAKA PFIALNMAAI
210 220 230 240 250
PKDLIESELF GHEKGAFTGA NTIRQGRFEQ ADGGTLFLDE IGDMPLDVQT
260 270 280 290 300
RLLRVLADGQ FYRVGGYAPV KVDVRIIAAT HQNLEQRVQE GKFREDLFHR
310 320 330 340 350
LNVIRVHLPP LRERREDIPR LARHFLQVAA RELGVEAKLL HPETEAALTR
360 370 380 390 400
LAWPGNVRQL ENTCRWLTVM AAGQEVLIQD LPGELFESTV AESTSQMQPD
410 420 430 440 450
SWATLLAQWA DRALRSGHQN LLSEAQPELE RTLLTTALRH TQGHKQEAAR
460
LLGWGRNTLT RKLKELGME
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 142 – 144 | GEA → AK in CAA28808 (PubMed:2882477).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28808.1 L19201 Genomic DNA Translation: AAB03002.1 U00096 Genomic DNA Translation: AAC76865.1 AP009048 Genomic DNA Translation: BAE77441.1 D16509 Genomic DNA Translation: BAA03962.1 |
PIRi | B30377, RGECGG |
RefSeqi | NP_418304.1, NC_000913.3 WP_001188777.1, NZ_SSZK01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC76865; AAC76865; b3868 BAE77441; BAE77441; BAE77441 |
GeneIDi | 58461340 948361 |
KEGGi | ecj:JW3839 eco:b3868 |
PATRICi | fig|1411691.4.peg.2843 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28808.1 L19201 Genomic DNA Translation: AAB03002.1 U00096 Genomic DNA Translation: AAC76865.1 AP009048 Genomic DNA Translation: BAE77441.1 D16509 Genomic DNA Translation: BAA03962.1 |
PIRi | B30377, RGECGG |
RefSeqi | NP_418304.1, NC_000913.3 WP_001188777.1, NZ_SSZK01000026.1 |
3D structure databases
BMRBi | P0AFB8 |
SMRi | P0AFB8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262628, 38 interactors 852658, 2 interactors |
DIPi | DIP-9781N |
IntActi | P0AFB8, 6 interactors |
STRINGi | 511145.b3868 |
Proteomic databases
jPOSTi | P0AFB8 |
PaxDbi | P0AFB8 |
PRIDEi | P0AFB8 |
Genome annotation databases
EnsemblBacteriai | AAC76865; AAC76865; b3868 BAE77441; BAE77441; BAE77441 |
GeneIDi | 58461340 948361 |
KEGGi | ecj:JW3839 eco:b3868 |
PATRICi | fig|1411691.4.peg.2843 |
Organism-specific databases
EchoBASEi | EB0380 |
Phylogenomic databases
eggNOGi | COG2204, Bacteria |
HOGENOMi | CLU_000445_0_1_6 |
InParanoidi | P0AFB8 |
PhylomeDBi | P0AFB8 |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-NRI |
Miscellaneous databases
PROi | PR:P0AFB8 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR011006, CheY-like_superfamily IPR009057, Homeobox-like_sf IPR002197, HTH_Fis IPR027417, P-loop_NTPase IPR001789, Sig_transdc_resp-reg_receiver IPR002078, Sigma_54_int IPR025662, Sigma_54_int_dom_ATP-bd_1 IPR025943, Sigma_54_int_dom_ATP-bd_2 IPR025944, Sigma_54_int_dom_CS IPR010114, Transcript_reg_NtrC |
Pfami | View protein in Pfam PF02954, HTH_8, 1 hit PF00072, Response_reg, 1 hit PF00158, Sigma54_activat, 1 hit |
PRINTSi | PR01590, HTHFIS |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit SSF52172, SSF52172, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR01818, ntrC, 1 hit |
PROSITEi | View protein in PROSITE PS50110, RESPONSE_REGULATORY, 1 hit PS00675, SIGMA54_INTERACT_1, 1 hit PS00676, SIGMA54_INTERACT_2, 1 hit PS00688, SIGMA54_INTERACT_3, 1 hit PS50045, SIGMA54_INTERACT_4, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NTRC_ECOLI | |
Accessioni | P0AFB8Primary (citable) accession number: P0AFB8 Secondary accession number(s): P06713, Q2M8G5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |