UniProtKB - P0AFB5 (NTRB_ECOLI)
Protein
Sensory histidine kinase/phosphatase NtrB
Gene
glnL
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system NtrB/NtrC, which controls expression of the nitrogen-regulated (ntr) genes in response to nitrogen limitation. Under conditions of nitrogen limitation, NtrB autophosphorylates and transfers the phosphoryl group to NtrC. In the presence of nitrogen, acts as a phosphatase that dephosphorylates and inactivates NtrC.4 Publications
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.2 Publications EC:2.7.13.3
Activity regulationi
The phosphatase and kinase activities are regulated by the nitrogen regulatory proteins P-II 1 (GlnB) and P-II 2 (GlnK) (PubMed:2874557, PubMed:2574599, PubMed:10074086, PubMed:10231487). GlnB acts by binding to NtrB, leading to activation of the phosphatase activity and inhibition of the kinase activity. Binding of GlnB to NtrB is allosterically controlled by the small-molecule effector 2-ketoglutarate. At low 2-ketoglutarate concentrations, GlnB interacts with NtrB, but not at high 2-ketoglutarate concentrations (PubMed:10074086). Uridylylation of GlnB also prevents interaction with NtrB (PubMed:7961766).5 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 329 | ATPBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- phosphoprotein phosphatase activity Source: EcoCyc
- phosphorelay sensor kinase activity Source: EcoCyc
GO - Biological processi
- nitrogen fixation Source: UniProtKB-KW
- protein autophosphorylation Source: EcoCyc
- regulation of nitrogen compound metabolic process Source: EcoCyc
- regulation of transcription, DNA-templated Source: InterPro
- signal transduction Source: EcoCyc
Keywordsi
Molecular function | Hydrolase, Kinase, Transferase |
Biological process | Nitrogen fixation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-NRII |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:glnL1 Publication Synonyms:glnR, ntrB1 Publication Ordered Locus Names:b3869, JW3840 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074820 | 1 – 349 | Sensory histidine kinase/phosphatase NtrBAdd BLAST | 349 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 139 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication | 1 |
Post-translational modificationi
Autophosphorylated.3 Publications
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AFB5 |
PaxDbi | P0AFB5 |
PRIDEi | P0AFB5 |
PTM databases
iPTMneti | P0AFB5 |
Interactioni
Binary interactionsi
Hide detailsP0AFB5
With | #Exp. | IntAct |
---|---|---|
glnB [P0A9Z1] | 5 | EBI-701156,EBI-551053 |
glnG [P0AFB8] | 3 | EBI-701156,EBI-1113197 |
glnK [P0AC55] | 5 | EBI-701156,EBI-559503 |
itself | 3 | EBI-701156,EBI-701156 |
tfaE [P09153] | 4 | EBI-701156,EBI-9133821 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4262629, 18 interactors 852657, 10 interactors |
DIPi | DIP-9784N |
IntActi | P0AFB5, 10 interactors |
STRINGi | 511145.b3869 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFB5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFB5 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 5 – 78 | PASPROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 136 – 349 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 214 |
Domaini
The C-terminal ATP-binding domain is required for both kinase and phosphatase activities and contains the GlnB binding site.1 Publication
Phylogenomic databases
eggNOGi | COG3852, Bacteria |
HOGENOMi | CLU_000445_114_39_6 |
InParanoidi | P0AFB5 |
PhylomeDBi | P0AFB5 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFB5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATGTQPDAG QILNSLINSI LLIDDNLAIH YANPAAQQLL AQSSRKLFGT
60 70 80 90 100
PLPELLSYFS LNIELMQESL EAGQGFTDNE VTLVIDGRSH ILSVTAQRMP
110 120 130 140 150
DGMILLEMAP MDNQRRLSQE QLQHAQQVAA RDLVRGLAHE IKNPLGGLRG
160 170 180 190 200
AAQLLSKALP DPSLLEYTKV IIEQADRLRN LVDRLLGPQL PGTRVTESIH
210 220 230 240 250
KVAERVVTLV SMELPDNVRL IRDYDPSLPE LAHDPDQIEQ VLLNIVRNAL
260 270 280 290 300
QALGPEGGEI ILRTRTAFQL TLHGERYRLA ARIDVEDNGP GIPPHLQDTL
310 320 330 340
FYPMVSGREG GTGLGLSIAR NLIDQHSGKI EFTSWPGHTE FSVYLPIRK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28807.1 L19201 Genomic DNA Translation: AAB03003.1 U00096 Genomic DNA Translation: AAC76866.1 AP009048 Genomic DNA Translation: BAE77440.1 K02176 Genomic DNA Translation: AAA23881.1 |
PIRi | A30377, RGECGL |
RefSeqi | NP_418305.1, NC_000913.3 WP_000190577.1, NZ_STEB01000017.1 |
Genome annotation databases
EnsemblBacteriai | AAC76866; AAC76866; b3869 BAE77440; BAE77440; BAE77440 |
GeneIDi | 58390863 948360 |
KEGGi | ecj:JW3840 eco:b3869 |
PATRICi | fig|1411691.4.peg.2842 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X05173 Genomic DNA Translation: CAA28807.1 L19201 Genomic DNA Translation: AAB03003.1 U00096 Genomic DNA Translation: AAC76866.1 AP009048 Genomic DNA Translation: BAE77440.1 K02176 Genomic DNA Translation: AAA23881.1 |
PIRi | A30377, RGECGL |
RefSeqi | NP_418305.1, NC_000913.3 WP_000190577.1, NZ_STEB01000017.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1R62 | X-ray | 1.60 | A | 190-349 | [»] | |
SMRi | P0AFB5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262629, 18 interactors 852657, 10 interactors |
DIPi | DIP-9784N |
IntActi | P0AFB5, 10 interactors |
STRINGi | 511145.b3869 |
PTM databases
iPTMneti | P0AFB5 |
Proteomic databases
jPOSTi | P0AFB5 |
PaxDbi | P0AFB5 |
PRIDEi | P0AFB5 |
Genome annotation databases
EnsemblBacteriai | AAC76866; AAC76866; b3869 BAE77440; BAE77440; BAE77440 |
GeneIDi | 58390863 948360 |
KEGGi | ecj:JW3840 eco:b3869 |
PATRICi | fig|1411691.4.peg.2842 |
Organism-specific databases
EchoBASEi | EB0382 |
Phylogenomic databases
eggNOGi | COG3852, Bacteria |
HOGENOMi | CLU_000445_114_39_6 |
InParanoidi | P0AFB5 |
PhylomeDBi | P0AFB5 |
Enzyme and pathway databases
BioCyci | EcoCyc:PROTEIN-NRII |
Miscellaneous databases
EvolutionaryTracei | P0AFB5 |
PROi | PR:P0AFB5 |
Family and domain databases
CDDi | cd00082, HisKA, 1 hit cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR004358, Sig_transdc_His_kin-like_C |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF47384, SSF47384, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NTRB_ECOLI | |
Accessioni | P0AFB5Primary (citable) accession number: P0AFB5 Secondary accession number(s): P06712, Q2M8G6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 1, 1988 |
Last sequence update: | January 1, 1988 | |
Last modified: | April 7, 2021 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references