UniProtKB - P0AFA2 (NARX_ECOLI)
Protein
Nitrate/nitrite sensor protein NarX
Gene
narX
Organism
Escherichia coli (strain K12)
Status
Functioni
Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the NarL protein and of both nitrate/nitrite to the NarP protein. NarX probably activates NarL and NarP by phosphorylation in the presence of nitrate. NarX also plays a negative role in controlling NarL activity, probably through dephosphorylation in the absence of nitrate.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphoprotein phosphatase activity Source: EcoCyc
- phosphorelay sensor kinase activity Source: EcoCyc
- protein dimerization activity Source: InterPro
GO - Biological processi
- cellular response to DNA damage stimulus Source: EcoliWiki
- cellular response to nitrate Source: EcoCyc
- cellular response to nitrite Source: EcoCyc
- nitrate assimilation Source: UniProtKB-KW
- protein autophosphorylation Source: EcoCyc
- protein dephosphorylation Source: EcoCyc
- signal transduction Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Nitrate assimilation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:NARX-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:narX Synonyms:narR Ordered Locus Names:b1222, JW1213 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 14 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 15 – 37 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 38 – 151 | PeriplasmicSequence analysisAdd BLAST | 114 | |
Transmembranei | 152 – 174 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 175 – 598 | CytoplasmicSequence analysisAdd BLAST | 424 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074812 | 1 – 598 | Nitrate/nitrite sensor protein NarXAdd BLAST | 598 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 399 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AFA2 |
PaxDbi | P0AFA2 |
PRIDEi | P0AFA2 |
Interactioni
Binary interactionsi
P0AFA2
With | #Exp. | IntAct |
---|---|---|
narL [P0AF28] | 2 | EBI-1112775,EBI-1122899 |
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 4263270, 17 interactors |
DIPi | DIP-35785N |
IntActi | P0AFA2, 5 interactors |
STRINGi | 511145.b1222 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AFA2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AFA2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 176 – 228 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 393 – 587 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 195 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3850, Bacteria |
HOGENOMi | CLU_000445_20_10_6 |
InParanoidi | P0AFA2 |
PhylomeDBi | P0AFA2 |
Family and domain databases
Gene3Di | 1.20.120.960, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR029095, NarX-like_N IPR042295, NarX-like_N_sf IPR016380, Sig_transdc_His_kin_NarX/NarQ IPR011712, Sig_transdc_His_kin_sub3_dim/P |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF07730, HisKA_3, 1 hit PF13675, PilJ, 1 hit |
PIRSFi | PIRSF003167, STHK_NarX/NarQ, 1 hit |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AFA2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKRCLSPLT LVNQVALIVL LSTAIGLAGM AVSGWLVQGV QGSAHAINKA
60 70 80 90 100
GSLRMQSYRL LAAVPLSEKD KPLIKEMEQT AFSAELTRAA ERDGQLAQLQ
110 120 130 140 150
GLQDYWRNEL IPALMRAQNR ETVSADVSQF VAGLDQLVSG FDRTTEMRIE
160 170 180 190 200
TVVLVHRVMA VFMALLLVFT IIWLRARLLQ PWRQLLAMAS AVSHRDFTQR
210 220 230 240 250
ANISGRNEMA MLGTALNNMS AELAESYAVL EQRVQEKTAG LEHKNQILSF
260 270 280 290 300
LWQANRRLHS RAPLCERLSP VLNGLQNLTL LRDIELRVYD TDDEENHQEF
310 320 330 340 350
TCQPDMTCDD KGCQLCPRGV LPVGDRGTTL KWRLADSHTQ YGILLATLPQ
360 370 380 390 400
GRHLSHDQQQ LVDTLVEQLT ATLALDRHQE RQQQLIVMEE RATIARELHD
410 420 430 440 450
SIAQSLSCMK MQVSCLQMQG DALPESSREL LSQIRNELNA SWAQLRELLT
460 470 480 490 500
TFRLQLTEPG LRPALEASCE EYSAKFGFPV KLDYQLPPRL VPSHQAIHLL
510 520 530 540 550
QIAREALSNA LKHSQASEVV VTVAQNDNQV KLTVQDNGCG VPENAIRSNH
560 570 580 590
YGMIIMRDRA QSLRGDCRVR RRESGGTEVV VTFIPEKTFT DVQGDTHE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 42 – 52 | GSAHAINKAGS → AAPMRSTKRDA (PubMed:2657652).CuratedAdd BLAST | 11 | |
Sequence conflicti | 42 – 52 | GSAHAINKAGS → AAPMRSTKRDA (PubMed:2668029).CuratedAdd BLAST | 11 | |
Sequence conflicti | 374 | A → G in AAA24198 (PubMed:2649492).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13360 Genomic DNA Translation: CAA31741.1 U00096 Genomic DNA Translation: AAC74306.1 AP009048 Genomic DNA Translation: BAA36090.1 X15996 Genomic DNA Translation: CAA34125.1 M24910 Genomic DNA Translation: AAA24198.1 X65715 Genomic DNA Translation: CAA46631.1 X69189 Genomic DNA Translation: CAA48934.1 |
PIRi | S26137, RGECNX |
RefSeqi | NP_415740.1, NC_000913.3 WP_000918073.1, NZ_STEB01000023.1 |
Genome annotation databases
EnsemblBacteriai | AAC74306; AAC74306; b1222 BAA36090; BAA36090; BAA36090 |
GeneIDi | 58390543 945788 |
KEGGi | ecj:JW1213 eco:b1222 |
PATRICi | fig|1411691.4.peg.1060 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X13360 Genomic DNA Translation: CAA31741.1 U00096 Genomic DNA Translation: AAC74306.1 AP009048 Genomic DNA Translation: BAA36090.1 X15996 Genomic DNA Translation: CAA34125.1 M24910 Genomic DNA Translation: AAA24198.1 X65715 Genomic DNA Translation: CAA46631.1 X69189 Genomic DNA Translation: CAA48934.1 |
PIRi | S26137, RGECNX |
RefSeqi | NP_415740.1, NC_000913.3 WP_000918073.1, NZ_STEB01000023.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3EZH | X-ray | 1.70 | A/B | 38-151 | [»] | |
3EZI | X-ray | 1.70 | A/B/C/D | 42-148 | [»] | |
SMRi | P0AFA2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263270, 17 interactors |
DIPi | DIP-35785N |
IntActi | P0AFA2, 5 interactors |
STRINGi | 511145.b1222 |
Proteomic databases
jPOSTi | P0AFA2 |
PaxDbi | P0AFA2 |
PRIDEi | P0AFA2 |
Genome annotation databases
EnsemblBacteriai | AAC74306; AAC74306; b1222 BAA36090; BAA36090; BAA36090 |
GeneIDi | 58390543 945788 |
KEGGi | ecj:JW1213 eco:b1222 |
PATRICi | fig|1411691.4.peg.1060 |
Organism-specific databases
EchoBASEi | EB0640 |
Phylogenomic databases
eggNOGi | COG3850, Bacteria |
HOGENOMi | CLU_000445_20_10_6 |
InParanoidi | P0AFA2 |
PhylomeDBi | P0AFA2 |
Enzyme and pathway databases
BioCyci | EcoCyc:NARX-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AFA2 |
PROi | PR:P0AFA2 |
Family and domain databases
Gene3Di | 1.20.120.960, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR029095, NarX-like_N IPR042295, NarX-like_N_sf IPR016380, Sig_transdc_His_kin_NarX/NarQ IPR011712, Sig_transdc_His_kin_sub3_dim/P |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF07730, HisKA_3, 1 hit PF13675, PilJ, 1 hit |
PIRSFi | PIRSF003167, STHK_NarX/NarQ, 1 hit |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit |
SUPFAMi | SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NARX_ECOLI | |
Accessioni | P0AFA2Primary (citable) accession number: P0AFA2 Secondary accession number(s): P10956 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references