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Protein

2-iminobutanoate/2-iminopropanoate deaminase

Gene

ridA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Accelerates the release of ammonia from reactive enamine/imine intermediates of the PLP-dependent threonine dehydratase (IlvA) in the low water environment of the cell. It catalyzes the deamination of enamine/imine intermediates to yield 2-ketobutyrate and ammonia. It is required for the detoxification of reactive intermediates of IlvA due to their highly nucleophilic abilities. Involved in the isoleucine biosynthesis (By similarity).By similarity

Catalytic activityi

2-iminobutanoate + H2O = 2-oxobutanoate + NH3.
2-iminopropanoate + H2O = pyruvate + NH3.

Pathwayi: L-isoleucine biosynthesis

This protein is involved in the subpathway that synthesizes 2-oxobutanoate from L-threonine. This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei17Stabilizes the substrateBy similarity1
Binding sitei105SubstrateSequence analysis1
Sitei120Important for catalytic activity at high pHBy similarity1

GO - Molecular functioni

  • deaminase activity Source: EcoCyc
  • identical protein binding Source: EcoCyc
  • unfolded protein binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Detoxification, Isoleucine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:G7877-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
2-iminobutanoate/2-iminopropanoate deaminase (EC:3.5.99.10)
Alternative name(s):
Enamine/imine deaminase
Gene namesi
Name:ridA
Synonyms:yjgF
Ordered Locus Names:b4243, JW5755
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12524 ridA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB03544 S-Phosphocysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001703212 – 1282-iminobutanoate/2-iminopropanoate deaminaseAdd BLAST127

Proteomic databases

EPDiP0AF93
PaxDbiP0AF93
PRIDEiP0AF93

2D gel databases

SWISS-2DPAGEiP0AF93

Interactioni

Subunit structurei

Homotrimer.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4262720, 14 interactors
DIPiDIP-36232N
STRINGi316385.ECDH10B_4438

Structurei

Secondary structure

1128
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AF93
SMRiP0AF93
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AF93

Family & Domainsi

Sequence similaritiesi

Belongs to the RutC family.Curated

Phylogenomic databases

eggNOGiENOG4105KME Bacteria
COG0251 LUCA
HOGENOMiHOG000267215
InParanoidiP0AF93
KOiK09022
PhylomeDBiP0AF93

Family and domain databases

Gene3Di3.30.1330.40, 1 hit
InterProiView protein in InterPro
IPR006056 RidA
IPR019897 RidA_CS
IPR035959 RutC-like_sf
IPR006175 YjgF/YER057c/UK114
PANTHERiPTHR11803 PTHR11803, 1 hit
PfamiView protein in Pfam
PF01042 Ribonuc_L-PSP, 1 hit
SUPFAMiSSF55298 SSF55298, 1 hit
TIGRFAMsiTIGR00004 TIGR00004, 1 hit
PROSITEiView protein in PROSITE
PS01094 UPF0076, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AF93-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSKTIATENA PAAIGPYVQG VDLGNMIITS GQIPVNPKTG EVPADVAAQA
60 70 80 90 100
RQSLDNVKAI VEAAGLKVGD IVKTTVFVKD LNDFATVNAT YEAFFTEHNA
110 120
TFPARSCVEV ARLPKDVKIE IEAIAVRR
Length:128
Mass (Da):13,612
Last modified:January 23, 2007 - v2
Checksum:i4696BE30BAA71A15
GO

Sequence cautioni

The sequence AAA97140 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97140.1 Different initiation.
U00096 Genomic DNA Translation: AAC77200.2
AP009048 Genomic DNA Translation: BAE78242.1
RefSeqiNP_418664.2, NC_000913.3
WP_000047539.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC77200; AAC77200; b4243
BAE78242; BAE78242; BAE78242
GeneIDi948771
KEGGiecj:JW5755
eco:b4243
PATRICifig|511145.12.peg.4376

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U14003 Genomic DNA Translation: AAA97140.1 Different initiation.
U00096 Genomic DNA Translation: AAC77200.2
AP009048 Genomic DNA Translation: BAE78242.1
RefSeqiNP_418664.2, NC_000913.3
WP_000047539.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QU9X-ray1.20A/B/C1-128[»]
ProteinModelPortaliP0AF93
SMRiP0AF93
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262720, 14 interactors
DIPiDIP-36232N
STRINGi316385.ECDH10B_4438

Chemistry databases

DrugBankiDB03544 S-Phosphocysteine

2D gel databases

SWISS-2DPAGEiP0AF93

Proteomic databases

EPDiP0AF93
PaxDbiP0AF93
PRIDEiP0AF93

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77200; AAC77200; b4243
BAE78242; BAE78242; BAE78242
GeneIDi948771
KEGGiecj:JW5755
eco:b4243
PATRICifig|511145.12.peg.4376

Organism-specific databases

EchoBASEiEB2415
EcoGeneiEG12524 ridA

Phylogenomic databases

eggNOGiENOG4105KME Bacteria
COG0251 LUCA
HOGENOMiHOG000267215
InParanoidiP0AF93
KOiK09022
PhylomeDBiP0AF93

Enzyme and pathway databases

BioCyciEcoCyc:G7877-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AF93
PROiPR:P0AF93

Family and domain databases

Gene3Di3.30.1330.40, 1 hit
InterProiView protein in InterPro
IPR006056 RidA
IPR019897 RidA_CS
IPR035959 RutC-like_sf
IPR006175 YjgF/YER057c/UK114
PANTHERiPTHR11803 PTHR11803, 1 hit
PfamiView protein in Pfam
PF01042 Ribonuc_L-PSP, 1 hit
SUPFAMiSSF55298 SSF55298, 1 hit
TIGRFAMsiTIGR00004 TIGR00004, 1 hit
PROSITEiView protein in PROSITE
PS01094 UPF0076, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRIDA_ECOLI
AccessioniPrimary (citable) accession number: P0AF93
Secondary accession number(s): P39330, P76806, Q2M664
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
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Main funding by: National Institutes of Health

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