UniProtKB - P0AF67 (TSAE_ECOLI)
Protein
tRNA threonylcarbamoyladenosine biosynthesis protein TsaE
Gene
tsaE
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t6A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.1 Publication
Miscellaneous
TsaBCDE are necessary and sufficient for tRNA(NNU) t6A37 threonylcarbamoyladenosine modification in vitro in E.coli.1 Publication
Caution
The well-known t6A modification appears to be a hydrolyzed artifact of natural cyclic t6A (ct6A) that occurs during the preparation and handling of tRNA in E.coli and many other species (PubMed:23242255). In these species, the t6A modification is processed further by dehydration into ct6A, a reaction catalyzed by TcdA.Curated
Kineticsi
kcat is 0.0026 sec(-1) for ATP hydrolysis.
- KM=169 µM for ATP1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | ATPBy similarity | 1 | |
Metal bindingi | 42 | MagnesiumBy similarity | 1 | |
Metal bindingi | 108 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 38 – 43 | ATPBy similarity | 6 |
GO - Molecular functioni
- ADP binding Source: EcoCyc
- ATPase activity Source: EcoCyc
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
- protein kinase activity Source: EcoCyc
GO - Biological processi
- protein autophosphorylation Source: EcoCyc
- tRNA threonylcarbamoyladenosine modification Source: EcoCyc
Keywordsi
Biological process | tRNA processing |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11757-MONOMER MetaCyc:EG11757-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA threonylcarbamoyladenosine biosynthesis protein TsaEAlternative name(s): t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE |
Gene namesi | Name:tsaE Synonyms:yjeE Ordered Locus Names:b4168, JW4126 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cytoplasm 1 Publication
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Appears essential for growth, since no null mutants can be obtained. Conditional depletion of this gene leads to very elongated cells, 1.5 to 1.75 times wider than wild-type, displaying an unusual distribution of the DNA, which appears to be located around the periphery of the cell rather than throughout the cytoplasm. The TsaE depletion phenotype is suppressed by overexpressing the response regulator RstA.1 Publication
Chemistry databases
ChEMBLi | CHEMBL3309030 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096205 | 1 – 153 | tRNA threonylcarbamoyladenosine biosynthesis protein TsaEAdd BLAST | 153 |
Proteomic databases
jPOSTi | P0AF67 |
PaxDbi | P0AF67 |
PRIDEi | P0AF67 |
Interactioni
Subunit structurei
Homodimer.
Interacts with TsaB.
2 PublicationsBinary interactionsi
P0AF67
With | #Exp. | IntAct |
---|---|---|
rlmB [P63177] | 3 | EBI-561268,EBI-562712 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4262705, 155 interactors 852976, 1 interactor |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-47867N |
IntActi | P0AF67, 23 interactors |
STRINGi | 511145.b4168 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TsaE family.Curated
Phylogenomic databases
eggNOGi | COG0802, Bacteria |
HOGENOMi | CLU_087829_2_2_6 |
InParanoidi | P0AF67 |
PhylomeDBi | P0AF67 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003442, T6A_TsaE |
Pfami | View protein in Pfam PF02367, TsaE, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00150, T6A_YjeE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AF67-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MMNRVIPLPD EQATLDLGER VAKACDGATV IYLYGDLGAG KTTFSRGFLQ
60 70 80 90 100
ALGHQGNVKS PTYTLVEPYT LDNLMVYHFD LYRLADPEEL EFMGIRDYFA
110 120 130 140 150
NDAICLVEWP QQGTGVLPDP DVEIHIDYQA QGREARVSAV SSAGELLLAR
LAG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19346 Unassigned DNA Translation: AAA20096.1 U14003 Genomic DNA Translation: AAA97064.1 U00096 Genomic DNA Translation: AAC77125.1 AP009048 Genomic DNA Translation: BAE78169.1 |
PIRi | S56393 |
RefSeqi | NP_418589.1, NC_000913.3 WP_000981977.1, NZ_STEB01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC77125; AAC77125; b4168 BAE78169; BAE78169; BAE78169 |
GeneIDi | 52078551 948684 |
KEGGi | ecj:JW4126 eco:b4168 |
PATRICi | fig|1411691.4.peg.2533 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L19346 Unassigned DNA Translation: AAA20096.1 U14003 Genomic DNA Translation: AAA97064.1 U00096 Genomic DNA Translation: AAC77125.1 AP009048 Genomic DNA Translation: BAE78169.1 |
PIRi | S56393 |
RefSeqi | NP_418589.1, NC_000913.3 WP_000981977.1, NZ_STEB01000013.1 |
3D structure databases
SMRi | P0AF67 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262705, 155 interactors 852976, 1 interactor |
ComplexPortali | CPX-1094, YgjD-YeaZ-YjeE complex |
DIPi | DIP-47867N |
IntActi | P0AF67, 23 interactors |
STRINGi | 511145.b4168 |
Chemistry databases
ChEMBLi | CHEMBL3309030 |
Proteomic databases
jPOSTi | P0AF67 |
PaxDbi | P0AF67 |
PRIDEi | P0AF67 |
Genome annotation databases
EnsemblBacteriai | AAC77125; AAC77125; b4168 BAE78169; BAE78169; BAE78169 |
GeneIDi | 52078551 948684 |
KEGGi | ecj:JW4126 eco:b4168 |
PATRICi | fig|1411691.4.peg.2533 |
Organism-specific databases
EchoBASEi | EB1707 |
Phylogenomic databases
eggNOGi | COG0802, Bacteria |
HOGENOMi | CLU_087829_2_2_6 |
InParanoidi | P0AF67 |
PhylomeDBi | P0AF67 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11757-MONOMER MetaCyc:EG11757-MONOMER |
Miscellaneous databases
PROi | PR:P0AF67 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR003442, T6A_TsaE |
Pfami | View protein in Pfam PF02367, TsaE, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00150, T6A_YjeE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TSAE_ECOLI | |
Accessioni | P0AF67Primary (citable) accession number: P0AF67 Secondary accession number(s): P31805, Q2M6D7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families