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UniProtKB - P0AF52 (GHXP_ECOLI)
Protein
Guanine/hypoxanthine permease GhxP
Gene
ghxP
Organism
Escherichia coli (strain K12)
Status
Functioni
High-affinity transporter for guanine and hypoxanthine.
1 PublicationKineticsi
- KM=1.6 µM for guanine1 Publication
- KM=11.2 µM for hypoxanthine1 Publication
- Vmax=4.0 nmol/min/mg enzyme with guanine as substrate1 Publication
- Vmax=2.7 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication
GO - Molecular functioni
- guanine transmembrane transporter activity Source: EcoCyc
- purine nucleobase transmembrane transporter activity Source: GO_Central
GO - Biological processi
- guanine import across plasma membrane Source: EcoCyc
- hypoxanthine transport Source: EcoCyc
Keywordsi
Biological process | Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11939-MONOMER |
SABIO-RKi | P0AF52 |
Protein family/group databases
TCDBi | 2.A.40.7.5, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine/hypoxanthine permease GhxP |
Gene namesi | Name:ghxP Synonyms:yjcD Ordered Locus Names:b4064, JW4025 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 25 | CytoplasmicSequence analysisAdd BLAST | 25 | |
Transmembranei | 26 – 49 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 50 – 59 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 60 – 78 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 79 – 80 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 81 – 97 | Discontinuously helicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 98 – 109 | PeriplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 110 – 129 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 130 – 141 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 142 – 162 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 163 – 180 | PeriplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 181 – 198 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 199 – 202 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 203 – 222 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 223 – 254 | PeriplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 255 – 283 | HelicalSequence analysisAdd BLAST | 29 | |
Topological domaini | 284 – 296 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 297 – 312 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 313 – 314 | PeriplasmicSequence analysis | 2 | |
Transmembranei | 315 – 330 | Discontinuously helicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 331 – 334 | CytoplasmicSequence analysis | 4 | |
Transmembranei | 335 – 349 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 350 – 360 | PeriplasmicSequence analysisAdd BLAST | 11 | |
Transmembranei | 361 – 380 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 381 – 385 | CytoplasmicSequence analysis | 5 | |
Intramembranei | 386 – 421 | Discontinuously helicalSequence analysisAdd BLAST | 36 | |
Topological domaini | 422 – 449 | CytoplasmicSequence analysisAdd BLAST | 28 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | T → A: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 35 | T → H: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 88 | A → G or S: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 271 | D → A or N: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 271 | D → E: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 275 | T → A, D or N: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 275 | T → S: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 302 | D → E: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 302 | D → N: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 321 | I → A: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 321 | I → E: Almost no change in activity. 1 Publication | 1 | |
Mutagenesisi | 322 | E → D: Strong decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 322 | E → Q: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 323 | S → A or N: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000169720 | 1 – 449 | Guanine/hypoxanthine permease GhxPAdd BLAST | 449 |
Proteomic databases
jPOSTi | P0AF52 |
PaxDbi | P0AF52 |
PRIDEi | P0AF52 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4262672, 293 interactors |
DIPi | DIP-48113N |
IntActi | P0AF52, 1 interactor |
STRINGi | 511145.b4064 |
Structurei
Family & Domainsi
Sequence similaritiesi
Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2252, Bacteria |
HOGENOMi | CLU_024508_0_1_6 |
InParanoidi | P0AF52 |
PhylomeDBi | P0AF52 |
Family and domain databases
InterProi | View protein in InterPro IPR045018, Azg-like IPR006043, NCS2 |
PANTHERi | PTHR43337, PTHR43337, 1 hit |
Pfami | View protein in Pfam PF00860, Xan_ur_permease, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AF52-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTPSARTGG SLDAWFKISQ RGSTVRQEVV AGLTTFLAMV YSVIVVPGML
60 70 80 90 100
GKAGFPPAAV FVATCLVAGL GSIVMGLWAN LPLAIGCAIS LTAFTAFSLV
110 120 130 140 150
LGQHISVPVA LGAVFLMGVL FTVISATGIR SWILRNLPHG VAHGTGIGIG
160 170 180 190 200
LFLLLIAANG VGLVIKNPLD GLPVALGDFA TFPVIMSLVG LAVIIGLEKL
210 220 230 240 250
KVPGGILLTI IGISIVGLIF DPNVHFSGVF AMPSLSDENG NSLIGSLDIM
260 270 280 290 300
GALNPVVLPS VLALVMTAVF DATGTIRAVA GQANLLDKDG QIIDGGKALT
310 320 330 340 350
TDSMSSVFSG LVGAAPAAVY IESAAGTAAG GKTGLTAITV GVLFLLILFL
360 370 380 390 400
SPLSYLVPGY ATAPALMYVG LLMLSNVAKI DFADFVDAMA GLVTAVFIVL
410 420 430 440
TCNIVTGIMI GFATLVIGRL VSGEWRKLNI GTVVIAVALV TFYAGGWAI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43158.1 U00096 Genomic DNA Translation: AAC77034.1 AP009048 Genomic DNA Translation: BAE78066.1 |
PIRi | G65214 |
RefSeqi | NP_418488.1, NC_000913.3 WP_000106882.1, NZ_STEB01000014.1 |
Genome annotation databases
EnsemblBacteriai | AAC77034; AAC77034; b4064 BAE78066; BAE78066; BAE78066 |
GeneIDi | 66672020 948565 |
KEGGi | ecj:JW4025 eco:b4064 |
PATRICi | fig|1411691.4.peg.2640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA Translation: AAC43158.1 U00096 Genomic DNA Translation: AAC77034.1 AP009048 Genomic DNA Translation: BAE78066.1 |
PIRi | G65214 |
RefSeqi | NP_418488.1, NC_000913.3 WP_000106882.1, NZ_STEB01000014.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4262672, 293 interactors |
DIPi | DIP-48113N |
IntActi | P0AF52, 1 interactor |
STRINGi | 511145.b4064 |
Protein family/group databases
TCDBi | 2.A.40.7.5, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family |
Proteomic databases
jPOSTi | P0AF52 |
PaxDbi | P0AF52 |
PRIDEi | P0AF52 |
Genome annotation databases
EnsemblBacteriai | AAC77034; AAC77034; b4064 BAE78066; BAE78066; BAE78066 |
GeneIDi | 66672020 948565 |
KEGGi | ecj:JW4025 eco:b4064 |
PATRICi | fig|1411691.4.peg.2640 |
Organism-specific databases
EchoBASEi | EB1883 |
Phylogenomic databases
eggNOGi | COG2252, Bacteria |
HOGENOMi | CLU_024508_0_1_6 |
InParanoidi | P0AF52 |
PhylomeDBi | P0AF52 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11939-MONOMER |
SABIO-RKi | P0AF52 |
Miscellaneous databases
PROi | PR:P0AF52 |
Family and domain databases
InterProi | View protein in InterPro IPR045018, Azg-like IPR006043, NCS2 |
PANTHERi | PTHR43337, PTHR43337, 1 hit |
Pfami | View protein in Pfam PF00860, Xan_ur_permease, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GHXP_ECOLI | |
Accessioni | P0AF52Primary (citable) accession number: P0AF52 Secondary accession number(s): P32702, Q2M6P0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families