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Entry version 116 (23 Feb 2022)
Sequence version 1 (20 Dec 2005)
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Protein

Guanine/hypoxanthine permease GhxP

Gene

ghxP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

High-affinity transporter for guanine and hypoxanthine.

1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1.6 µM for guanine1 Publication
  2. KM=11.2 µM for hypoxanthine1 Publication
  1. Vmax=4.0 nmol/min/mg enzyme with guanine as substrate1 Publication
  2. Vmax=2.7 nmol/min/mg enzyme with hypoxanthine as substrate1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

  • guanine transmembrane transporter activity Source: EcoCyc
  • purine nucleobase transmembrane transporter activity Source: GO_Central

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:EG11939-MONOMER

SABIO-RK: Biochemical Reaction Kinetics Database

More...
SABIO-RKi
P0AF52

Protein family/group databases

Transport Classification Database

More...
TCDBi
2.A.40.7.5, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Guanine/hypoxanthine permease GhxP
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:ghxP
Synonyms:yjcD
Ordered Locus Names:b4064, JW4025
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 25CytoplasmicSequence analysisAdd BLAST25
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei26 – 49HelicalSequence analysisAdd BLAST24
Topological domaini50 – 59PeriplasmicSequence analysis10
Transmembranei60 – 78HelicalSequence analysisAdd BLAST19
Topological domaini79 – 80CytoplasmicSequence analysis2
Transmembranei81 – 97Discontinuously helicalSequence analysisAdd BLAST17
Topological domaini98 – 109PeriplasmicSequence analysisAdd BLAST12
Transmembranei110 – 129HelicalSequence analysisAdd BLAST20
Topological domaini130 – 141CytoplasmicSequence analysisAdd BLAST12
Transmembranei142 – 162HelicalSequence analysisAdd BLAST21
Topological domaini163 – 180PeriplasmicSequence analysisAdd BLAST18
Transmembranei181 – 198HelicalSequence analysisAdd BLAST18
Topological domaini199 – 202CytoplasmicSequence analysis4
Transmembranei203 – 222HelicalSequence analysisAdd BLAST20
Topological domaini223 – 254PeriplasmicSequence analysisAdd BLAST32
Transmembranei255 – 283HelicalSequence analysisAdd BLAST29
Topological domaini284 – 296CytoplasmicSequence analysisAdd BLAST13
Transmembranei297 – 312HelicalSequence analysisAdd BLAST16
Topological domaini313 – 314PeriplasmicSequence analysis2
Transmembranei315 – 330Discontinuously helicalSequence analysisAdd BLAST16
Topological domaini331 – 334CytoplasmicSequence analysis4
Transmembranei335 – 349HelicalSequence analysisAdd BLAST15
Topological domaini350 – 360PeriplasmicSequence analysisAdd BLAST11
Transmembranei361 – 380HelicalSequence analysisAdd BLAST20
Topological domaini381 – 385CytoplasmicSequence analysis5
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a region that is buried within a membrane, but does not cross it.<p><a href='/help/intramem' target='_top'>More...</a></p>Intramembranei386 – 421Discontinuously helicalSequence analysisAdd BLAST36
Topological domaini422 – 449CytoplasmicSequence analysisAdd BLAST28

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi35T → A: Almost no change in activity. 1 Publication1
Mutagenesisi35T → H: Lack of activity. 1 Publication1
Mutagenesisi88A → G or S: Almost no change in activity. 1 Publication1
Mutagenesisi271D → A or N: Strong decrease in activity. 1 Publication1
Mutagenesisi271D → E: Lack of activity. 1 Publication1
Mutagenesisi275T → A, D or N: Lack of activity. 1 Publication1
Mutagenesisi275T → S: Strong decrease in activity. 1 Publication1
Mutagenesisi302D → E: Lack of activity. 1 Publication1
Mutagenesisi302D → N: Almost no change in activity. 1 Publication1
Mutagenesisi321I → A: Lack of activity. 1 Publication1
Mutagenesisi321I → E: Almost no change in activity. 1 Publication1
Mutagenesisi322E → D: Strong decrease in activity. 1 Publication1
Mutagenesisi322E → Q: Lack of activity. 1 Publication1
Mutagenesisi323S → A or N: Lack of activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001697201 – 449Guanine/hypoxanthine permease GhxPAdd BLAST449

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P0AF52

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P0AF52

PRoteomics IDEntifications database

More...
PRIDEi
P0AF52

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
4262672, 293 interactors

Database of interacting proteins

More...
DIPi
DIP-48113N

Protein interaction database and analysis system

More...
IntActi
P0AF52, 1 interactor

STRING: functional protein association networks

More...
STRINGi
511145.b4064

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG2252, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_024508_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P0AF52

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P0AF52

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR045018, Azg-like
IPR006043, NCS2

The PANTHER Classification System

More...
PANTHERi
PTHR43337, PTHR43337, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00860, Xan_ur_permease, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AF52-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTPSARTGG SLDAWFKISQ RGSTVRQEVV AGLTTFLAMV YSVIVVPGML
60 70 80 90 100
GKAGFPPAAV FVATCLVAGL GSIVMGLWAN LPLAIGCAIS LTAFTAFSLV
110 120 130 140 150
LGQHISVPVA LGAVFLMGVL FTVISATGIR SWILRNLPHG VAHGTGIGIG
160 170 180 190 200
LFLLLIAANG VGLVIKNPLD GLPVALGDFA TFPVIMSLVG LAVIIGLEKL
210 220 230 240 250
KVPGGILLTI IGISIVGLIF DPNVHFSGVF AMPSLSDENG NSLIGSLDIM
260 270 280 290 300
GALNPVVLPS VLALVMTAVF DATGTIRAVA GQANLLDKDG QIIDGGKALT
310 320 330 340 350
TDSMSSVFSG LVGAAPAAVY IESAAGTAAG GKTGLTAITV GVLFLLILFL
360 370 380 390 400
SPLSYLVPGY ATAPALMYVG LLMLSNVAKI DFADFVDAMA GLVTAVFIVL
410 420 430 440
TCNIVTGIMI GFATLVIGRL VSGEWRKLNI GTVVIAVALV TFYAGGWAI
Length:449
Mass (Da):45,711
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iBDBFE2F057E45910
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43158.1
U00096 Genomic DNA Translation: AAC77034.1
AP009048 Genomic DNA Translation: BAE78066.1

Protein sequence database of the Protein Information Resource

More...
PIRi
G65214

NCBI Reference Sequences

More...
RefSeqi
NP_418488.1, NC_000913.3
WP_000106882.1, NZ_STEB01000014.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC77034; AAC77034; b4064
BAE78066; BAE78066; BAE78066

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
66672020
948565

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW4025
eco:b4064

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.2640

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43158.1
U00096 Genomic DNA Translation: AAC77034.1
AP009048 Genomic DNA Translation: BAE78066.1
PIRiG65214
RefSeqiNP_418488.1, NC_000913.3
WP_000106882.1, NZ_STEB01000014.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4262672, 293 interactors
DIPiDIP-48113N
IntActiP0AF52, 1 interactor
STRINGi511145.b4064

Protein family/group databases

TCDBi2.A.40.7.5, the nucleobase/ascorbate transporter (nat) or nucleobase:cation symporter-2 (ncs2) family

Proteomic databases

jPOSTiP0AF52
PaxDbiP0AF52
PRIDEiP0AF52

Genome annotation databases

EnsemblBacteriaiAAC77034; AAC77034; b4064
BAE78066; BAE78066; BAE78066
GeneIDi66672020
948565
KEGGiecj:JW4025
eco:b4064
PATRICifig|1411691.4.peg.2640

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB1883

Phylogenomic databases

eggNOGiCOG2252, Bacteria
HOGENOMiCLU_024508_0_1_6
InParanoidiP0AF52
PhylomeDBiP0AF52

Enzyme and pathway databases

BioCyciEcoCyc:EG11939-MONOMER
SABIO-RKiP0AF52

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P0AF52

Family and domain databases

InterProiView protein in InterPro
IPR045018, Azg-like
IPR006043, NCS2
PANTHERiPTHR43337, PTHR43337, 1 hit
PfamiView protein in Pfam
PF00860, Xan_ur_permease, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiGHXP_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AF52
Secondary accession number(s): P32702, Q2M6P0
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: February 23, 2022
This is version 116 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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