Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nitrate/nitrite response regulator protein NarL

Gene

narL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein activates the expression of the nitrate reductase (narGHJI) and formate dehydrogenase-N (fdnGHI) operons and represses the transcription of the fumarate reductase (frdABCD) operon in response to a nitrate/nitrite induction signal transmitted by either the NarX or NarQ proteins.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi173 – 192H-T-H motifPROSITE-ProRule annotationAdd BLAST20

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • bacterial-type RNA polymerase transcriptional repressor activity, sequence-specific DNA binding Source: CollecTF
  • DNA binding Source: EcoCyc
  • transcription regulatory region sequence-specific DNA binding Source: CollecTF

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processNitrate assimilation, Transcription, Transcription regulation, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:NARL-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate/nitrite response regulator protein NarL
Gene namesi
Name:narL
Synonyms:frdR
Ordered Locus Names:b1221, JW1212
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10643 narL

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000811451 – 216Nitrate/nitrite response regulator protein NarLAdd BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP0AF28
PaxDbiP0AF28
PRIDEiP0AF28

Expressioni

Gene expression databases

CollecTFiEXPREG_00000880

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
narXP0AFA22EBI-1122899,EBI-1112775

Protein-protein interaction databases

BioGridi4261632, 23 interactors
DIPiDIP-35941N
IntActiP0AF28, 5 interactors
STRINGi316385.ECDH10B_1280

Structurei

Secondary structure

1216
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AF28
SMRiP0AF28
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AF28

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 124Response regulatoryPROSITE-ProRule annotationAdd BLAST117
Domaini149 – 214HTH luxR-typePROSITE-ProRule annotationAdd BLAST66

Phylogenomic databases

eggNOGiENOG4105WH1 Bacteria
COG2197 LUCA
HOGENOMiHOG000034813
InParanoidiP0AF28
KOiK07684
PhylomeDBiP0AF28

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
cd00156 REC, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR001789 Sig_transdc_resp-reg_receiver
IPR000792 Tscrpt_reg_LuxR_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00196 GerE, 1 hit
PF00072 Response_reg, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

P0AF28-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNQEPATIL LIDDHPMLRT GVKQLISMAP DITVVGEASN GEQGIELAES
60 70 80 90 100
LDPDLILLDL NMPGMNGLET LDKLREKSLS GRIVVFSVSN HEEDVVTALK
110 120 130 140 150
RGADGYLLKD MEPEDLLKAL HQAAAGEMVL SEALTPVLAA SLRANRATTE
160 170 180 190 200
RDVNQLTPRE RDILKLIAQG LPNKMIARRL DITESTVKVH VKHMLKKMKL
210
KSRVEAAVWV HQERIF
Length:216
Mass (Da):23,927
Last modified:December 20, 2005 - v1
Checksum:i7DED78E4EF7EC57D
GO

Sequence cautioni

The sequence CAA33023 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti115D → H in AAA24199 (PubMed:2649492).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13360 Genomic DNA Translation: CAA31742.1
M24910 Genomic DNA Translation: AAA24199.1
X14884 Genomic DNA Translation: CAA33023.1 Different initiation.
X69189 Genomic DNA Translation: CAA48935.1
U00096 Genomic DNA Translation: AAC74305.1
AP009048 Genomic DNA Translation: BAA36089.1
PIRiS09285 RGECNL
RefSeqiNP_415739.1, NC_000913.3
WP_000070491.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74305; AAC74305; b1221
BAA36089; BAA36089; BAA36089
GeneIDi945795
KEGGiecj:JW1212
eco:b1221
PATRICifig|1411691.4.peg.1061

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13360 Genomic DNA Translation: CAA31742.1
M24910 Genomic DNA Translation: AAA24199.1
X14884 Genomic DNA Translation: CAA33023.1 Different initiation.
X69189 Genomic DNA Translation: CAA48935.1
U00096 Genomic DNA Translation: AAC74305.1
AP009048 Genomic DNA Translation: BAA36089.1
PIRiS09285 RGECNL
RefSeqiNP_415739.1, NC_000913.3
WP_000070491.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A04X-ray2.20A/B2-216[»]
1JE8X-ray2.12A/B/E/F147-216[»]
1RNLX-ray2.40A2-216[»]
1ZG1X-ray2.30A/B/E/F147-216[»]
1ZG5X-ray2.30A/B/E/F147-216[»]
ProteinModelPortaliP0AF28
SMRiP0AF28
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261632, 23 interactors
DIPiDIP-35941N
IntActiP0AF28, 5 interactors
STRINGi316385.ECDH10B_1280

Proteomic databases

EPDiP0AF28
PaxDbiP0AF28
PRIDEiP0AF28

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74305; AAC74305; b1221
BAA36089; BAA36089; BAA36089
GeneIDi945795
KEGGiecj:JW1212
eco:b1221
PATRICifig|1411691.4.peg.1061

Organism-specific databases

EchoBASEiEB0637
EcoGeneiEG10643 narL

Phylogenomic databases

eggNOGiENOG4105WH1 Bacteria
COG2197 LUCA
HOGENOMiHOG000034813
InParanoidiP0AF28
KOiK07684
PhylomeDBiP0AF28

Enzyme and pathway databases

BioCyciEcoCyc:NARL-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AF28
PROiPR:P0AF28

Gene expression databases

CollecTFiEXPREG_00000880

Family and domain databases

CDDicd06170 LuxR_C_like, 1 hit
cd00156 REC, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR016032 Sig_transdc_resp-reg_C-effctor
IPR001789 Sig_transdc_resp-reg_receiver
IPR000792 Tscrpt_reg_LuxR_C
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF00196 GerE, 1 hit
PF00072 Response_reg, 1 hit
PRINTSiPR00038 HTHLUXR
SMARTiView protein in SMART
SM00421 HTH_LUXR, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF46894 SSF46894, 1 hit
SSF52172 SSF52172, 1 hit
PROSITEiView protein in PROSITE
PS00622 HTH_LUXR_1, 1 hit
PS50043 HTH_LUXR_2, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNARL_ECOLI
AccessioniPrimary (citable) accession number: P0AF28
Secondary accession number(s): P10957
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again