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Protein

Lipid II flippase MurJ

Gene

murJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.UniRule annotation3 Publications

Miscellaneous

FtsW (AC P0ABG4) is also proposed to act as a lipid II flippase. The identity of the lipid II flippase is controversial with conflicting in vivo and in vitro results.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

GO - Molecular functioni

  • lipid-linked peptidoglycan transporter activity Source: EcoCyc

GO - Biological processi

Keywordsi

Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Transport

Enzyme and pathway databases

BioCyciEcoCyc:G6561-MONOMER
MetaCyc:G6561-MONOMER
UniPathwayi
UPA00219

Protein family/group databases

TCDBi2.A.66.4.3 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid II flippase MurJCurated
Alternative name(s):
Peptidoglycan biosynthesis protein MurJCurated
Gene namesi
Name:murJ1 PublicationUniRule annotation
Synonyms:mviN, yceN
Ordered Locus Names:b1069, JW1056
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13880 murJ

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5CytoplasmicSequence analysis5
Transmembranei6 – 32HelicalSequence analysisAdd BLAST27
Topological domaini33 – 40PeriplasmicSequence analysis8
Transmembranei41 – 69HelicalSequence analysisAdd BLAST29
Topological domaini70 – 82CytoplasmicSequence analysisAdd BLAST13
Transmembranei83 – 109HelicalSequence analysisAdd BLAST27
Topological domaini110 – 123PeriplasmicSequence analysisAdd BLAST14
Transmembranei124 – 154HelicalSequence analysisAdd BLAST31
Topological domaini155 – 156CytoplasmicSequence analysis2
Transmembranei157 – 178HelicalSequence analysisAdd BLAST22
Topological domaini179 – 185PeriplasmicSequence analysis7
Transmembranei186 – 207HelicalSequence analysisAdd BLAST22
Topological domaini208 – 223CytoplasmicSequence analysisAdd BLAST16
Transmembranei224 – 253HelicalSequence analysisAdd BLAST30
Topological domaini254 – 263PeriplasmicSequence analysis10
Transmembranei264 – 292HelicalSequence analysisAdd BLAST29
Topological domaini293 – 302CytoplasmicSequence analysis10
Transmembranei303 – 337HelicalSequence analysisAdd BLAST35
Topological domaini338 – 346PeriplasmicSequence analysis9
Transmembranei347 – 374HelicalSequence analysisAdd BLAST28
Topological domaini375 – 379CytoplasmicSequence analysis5
Transmembranei380 – 402HelicalSequence analysisAdd BLAST23
Topological domaini403 – 409PeriplasmicSequence analysis7
Transmembranei410 – 429HelicalSequence analysisAdd BLAST20
Topological domaini430 – 443CytoplasmicSequence analysisAdd BLAST14
Transmembranei444 – 463HelicalSequence analysisAdd BLAST20
Topological domaini464 – 477PeriplasmicSequence analysisAdd BLAST14
Transmembranei478 – 499HelicalSequence analysisAdd BLAST22
Topological domaini500 – 511CytoplasmicSequence analysisAdd BLAST12

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18R → A or C: Lack of activity. 1 Publication1
Mutagenesisi24R → A or C: Lack of activity. 1 Publication1
Mutagenesisi39D → A or C: Lack of activity. 1 Publication1
Mutagenesisi52R → A: Decrease in activity. 1 Publication1
Mutagenesisi52R → C: Lack of activity. 1 Publication1
Mutagenesisi270R → A or C: Lack of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001820061 – 511Lipid II flippase MurJAdd BLAST511

Proteomic databases

PaxDbiP0AF16
PRIDEiP0AF16

Interactioni

Protein-protein interaction databases

BioGridi4260079, 316 interactors
IntActiP0AF16, 1 interactor
STRINGi316385.ECDH10B_1140

Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AF16
SMRiP0AF16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MurJ/MviN family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CJR Bacteria
COG0728 LUCA
HOGENOMiHOG000263812
InParanoidiP0AF16
KOiK03980
PhylomeDBiP0AF16

Family and domain databases

CDDicd13123 MATE_MurJ_like, 1 hit
HAMAPiMF_02078 MurJ_MviN, 1 hit
InterProiView protein in InterPro
IPR004268 MurJ
PfamiView protein in Pfam
PF03023 MVIN, 1 hit
PIRSFiPIRSF002869 MviN, 1 hit
PRINTSiPR01806 VIRFACTRMVIN
TIGRFAMsiTIGR01695 murJ_mviN, 1 hit

Sequencei

Sequence statusi: Complete.

P0AF16-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLLKSLAAV SSMTMFSRVL GFARDAIVAR IFGAGMATDA FFVAFKLPNL
60 70 80 90 100
LRRIFAEGAF SQAFVPILAE YKSKQGEDAT RVFVSYVSGL LTLALAVVTV
110 120 130 140 150
AGMLAAPWVI MVTAPGFADT ADKFALTSQL LKITFPYILL ISLASLVGAI
160 170 180 190 200
LNTWNRFSIP AFAPTLLNIS MIGFALFAAP YFNPPVLALA WAVTVGGVLQ
210 220 230 240 250
LVYQLPHLKK IGMLVLPRIN FHDAGAMRVV KQMGPAILGV SVSQISLIIN
260 270 280 290 300
TIFASFLASG SVSWMYYADR LMEFPSGVLG VALGTILLPS LSKSFASGNH
310 320 330 340 350
DEYNRLMDWG LRLCFLLALP SAVALGILSG PLTVSLFQYG KFTAFDALMT
360 370 380 390 400
QRALIAYSVG LIGLIVVKVL APGFYSRQDI KTPVKIAIVT LILTQLMNLA
410 420 430 440 450
FIGPLKHAGL SLSIGLAACL NASLLYWQLR KQKIFTPQPG WMAFLLRLVV
460 470 480 490 500
AVLVMSGVLL GMLHIMPEWS LGTMPWRLLR LMAVVLAGIA AYFAALAVLG
510
FKVKEFARRT V
Length:511
Mass (Da):55,267
Last modified:December 20, 2005 - v1
Checksum:iCB20FE3CFC9419C2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74153.1
AP009048 Genomic DNA Translation: BAA35877.1
PIRiB64850
RefSeqiNP_415587.1, NC_000913.3
WP_001050683.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74153; AAC74153; b1069
BAA35877; BAA35877; BAA35877
GeneIDi945487
KEGGiecj:JW1056
eco:b1069
PATRICifig|1411691.4.peg.1199

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74153.1
AP009048 Genomic DNA Translation: BAA35877.1
PIRiB64850
RefSeqiNP_415587.1, NC_000913.3
WP_001050683.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6CC4X-ray3.50A4-511[»]
ProteinModelPortaliP0AF16
SMRiP0AF16
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260079, 316 interactors
IntActiP0AF16, 1 interactor
STRINGi316385.ECDH10B_1140

Protein family/group databases

TCDBi2.A.66.4.3 the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Proteomic databases

PaxDbiP0AF16
PRIDEiP0AF16

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74153; AAC74153; b1069
BAA35877; BAA35877; BAA35877
GeneIDi945487
KEGGiecj:JW1056
eco:b1069
PATRICifig|1411691.4.peg.1199

Organism-specific databases

EchoBASEiEB3639
EcoGeneiEG13880 murJ

Phylogenomic databases

eggNOGiENOG4105CJR Bacteria
COG0728 LUCA
HOGENOMiHOG000263812
InParanoidiP0AF16
KOiK03980
PhylomeDBiP0AF16

Enzyme and pathway databases

UniPathwayi
UPA00219

BioCyciEcoCyc:G6561-MONOMER
MetaCyc:G6561-MONOMER

Miscellaneous databases

PROiPR:P0AF16

Family and domain databases

CDDicd13123 MATE_MurJ_like, 1 hit
HAMAPiMF_02078 MurJ_MviN, 1 hit
InterProiView protein in InterPro
IPR004268 MurJ
PfamiView protein in Pfam
PF03023 MVIN, 1 hit
PIRSFiPIRSF002869 MviN, 1 hit
PRINTSiPR01806 VIRFACTRMVIN
TIGRFAMsiTIGR01695 murJ_mviN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMURJ_ECOLI
AccessioniPrimary (citable) accession number: P0AF16
Secondary accession number(s): P75932
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: November 7, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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