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UniProtKB - P0AF16 (MURJ_ECOLI)

Entry version 110 (07 Apr 2021)
Sequence version 1 (20 Dec 2005)
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Protein

Lipid II flippase MurJ

Gene

murJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.UniRule annotation3 Publications

Miscellaneous

FtsW (AC P0ABG4) is also proposed to act as a lipid II flippase. The identity of the lipid II flippase is controversial with conflicting in vivo and in vitro results.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:G6561-MONOMER
MetaCyc:G6561-MONOMER

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...UniPathwayi		UPA00219

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.66.4.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Lipid II flippase MurJCurated
Alternative name(s):
Peptidoglycan biosynthesis protein MurJCurated
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:murJ1 PublicationUniRule annotation
Synonyms:mviN, yceN
Ordered Locus Names:b1069, JW1056
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 5CytoplasmicSequence analysis5
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei6 – 32HelicalSequence analysisAdd BLAST27
Topological domaini33 – 40PeriplasmicSequence analysis8
Transmembranei41 – 69HelicalSequence analysisAdd BLAST29
Topological domaini70 – 82CytoplasmicSequence analysisAdd BLAST13
Transmembranei83 – 109HelicalSequence analysisAdd BLAST27
Topological domaini110 – 123PeriplasmicSequence analysisAdd BLAST14
Transmembranei124 – 154HelicalSequence analysisAdd BLAST31
Topological domaini155 – 156CytoplasmicSequence analysis2
Transmembranei157 – 178HelicalSequence analysisAdd BLAST22
Topological domaini179 – 185PeriplasmicSequence analysis7
Transmembranei186 – 207HelicalSequence analysisAdd BLAST22
Topological domaini208 – 223CytoplasmicSequence analysisAdd BLAST16
Transmembranei224 – 253HelicalSequence analysisAdd BLAST30
Topological domaini254 – 263PeriplasmicSequence analysis10
Transmembranei264 – 292HelicalSequence analysisAdd BLAST29
Topological domaini293 – 302CytoplasmicSequence analysis10
Transmembranei303 – 337HelicalSequence analysisAdd BLAST35
Topological domaini338 – 346PeriplasmicSequence analysis9
Transmembranei347 – 374HelicalSequence analysisAdd BLAST28
Topological domaini375 – 379CytoplasmicSequence analysis5
Transmembranei380 – 402HelicalSequence analysisAdd BLAST23
Topological domaini403 – 409PeriplasmicSequence analysis7
Transmembranei410 – 429HelicalSequence analysisAdd BLAST20
Topological domaini430 – 443CytoplasmicSequence analysisAdd BLAST14
Transmembranei444 – 463HelicalSequence analysisAdd BLAST20
Topological domaini464 – 477PeriplasmicSequence analysisAdd BLAST14
Transmembranei478 – 499HelicalSequence analysisAdd BLAST22
Topological domaini500 – 511CytoplasmicSequence analysisAdd BLAST12

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi18R → A or C: Lack of activity. 1 Publication1
Mutagenesisi24R → A or C: Lack of activity. 1 Publication1
Mutagenesisi39D → A or C: Lack of activity. 1 Publication1
Mutagenesisi52R → A: Decrease in activity. 1 Publication1
Mutagenesisi52R → C: Lack of activity. 1 Publication1
Mutagenesisi270R → A or C: Lack of activity. 1 Publication1

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001820061 – 511Lipid II flippase MurJAdd BLAST511

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AF16

PRoteomics IDEntifications database

More...PRIDEi		P0AF16

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4260079, 316 interactors

Protein interaction database and analysis system

More...IntActi		P0AF16, 1 interactor

STRING: functional protein association networks

More...STRINGi		511145.b1069

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1511
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AF16

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the MurJ/MviN family.UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0728, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_006797_5_3_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AF16

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AF16

Family and domain databases

Conserved Domains Database

More...CDDi		cd13123, MATE_MurJ_like, 1 hit

HAMAP database of protein families

More...HAMAPi		MF_02078, MurJ_MviN, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR004268, MurJ

Pfam protein domain database

More...Pfami		View protein in Pfam
PF03023, MurJ, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF002869, MviN, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR01806, VIRFACTRMVIN

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR01695, murJ_mviN, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AF16-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLLKSLAAV SSMTMFSRVL GFARDAIVAR IFGAGMATDA FFVAFKLPNL 
        60         70         80         90        100
LRRIFAEGAF SQAFVPILAE YKSKQGEDAT RVFVSYVSGL LTLALAVVTV 
       110        120        130        140        150
AGMLAAPWVI MVTAPGFADT ADKFALTSQL LKITFPYILL ISLASLVGAI 
       160        170        180        190        200
LNTWNRFSIP AFAPTLLNIS MIGFALFAAP YFNPPVLALA WAVTVGGVLQ 
       210        220        230        240        250
LVYQLPHLKK IGMLVLPRIN FHDAGAMRVV KQMGPAILGV SVSQISLIIN 
       260        270        280        290        300
TIFASFLASG SVSWMYYADR LMEFPSGVLG VALGTILLPS LSKSFASGNH 
       310        320        330        340        350
DEYNRLMDWG LRLCFLLALP SAVALGILSG PLTVSLFQYG KFTAFDALMT 
       360        370        380        390        400
QRALIAYSVG LIGLIVVKVL APGFYSRQDI KTPVKIAIVT LILTQLMNLA 
       410        420        430        440        450
FIGPLKHAGL SLSIGLAACL NASLLYWQLR KQKIFTPQPG WMAFLLRLVV 
       460        470        480        490        500
AVLVMSGVLL GMLHIMPEWS LGTMPWRLLR LMAVVLAGIA AYFAALAVLG 
       510 
FKVKEFARRT V
Length:511
Mass (Da):55,267
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iCB20FE3CFC9419C2
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74153.1
AP009048 Genomic DNA Translation: BAA35877.1

Protein sequence database of the Protein Information Resource

More...PIRi		B64850

NCBI Reference Sequences

More...RefSeqi		NP_415587.1, NC_000913.3
WP_001050683.1, NZ_SSZK01000053.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC74153; AAC74153; b1069
BAA35877; BAA35877; BAA35877

Database of genes from NCBI RefSeq genomes

More...GeneIDi		945487

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW1056
eco:b1069

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.1199

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74153.1
AP009048 Genomic DNA Translation: BAA35877.1
PIRiB64850
RefSeqiNP_415587.1, NC_000913.3
WP_001050683.1, NZ_SSZK01000053.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6CC4X-ray3.50A4-511[»]
SMRiP0AF16
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4260079, 316 interactors
IntActiP0AF16, 1 interactor
STRINGi511145.b1069

Protein family/group databases

TCDBi2.A.66.4.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily

Proteomic databases

PaxDbiP0AF16
PRIDEiP0AF16

Genome annotation databases

EnsemblBacteriaiAAC74153; AAC74153; b1069
BAA35877; BAA35877; BAA35877
GeneIDi945487
KEGGiecj:JW1056
eco:b1069
PATRICifig|1411691.4.peg.1199

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB3639

Phylogenomic databases

eggNOGiCOG0728, Bacteria
HOGENOMiCLU_006797_5_3_6
InParanoidiP0AF16
PhylomeDBiP0AF16

Enzyme and pathway databases

UniPathwayiUPA00219
BioCyciEcoCyc:G6561-MONOMER
MetaCyc:G6561-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P0AF16

Family and domain databases

CDDicd13123, MATE_MurJ_like, 1 hit
HAMAPiMF_02078, MurJ_MviN, 1 hit
InterProiView protein in InterPro
IPR004268, MurJ
PfamiView protein in Pfam
PF03023, MurJ, 1 hit
PIRSFiPIRSF002869, MviN, 1 hit
PRINTSiPR01806, VIRFACTRMVIN
TIGRFAMsiTIGR01695, murJ_mviN, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiMURJ_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AF16
Secondary accession number(s): P75932
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: April 7, 2021
This is version 110 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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