UniProtKB - P0AF16 (MURJ_ECOLI)
Protein
Lipid II flippase MurJ
Gene
murJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in peptidoglycan biosynthesis. Transports lipid-linked peptidoglycan precursors from the inner to the outer leaflet of the cytoplasmic membrane.UniRule annotation3 Publications
Miscellaneous
FtsW (AC P0ABG4) is also proposed to act as a lipid II flippase. The identity of the lipid II flippase is controversial with conflicting in vivo and in vitro results.
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
GO - Molecular functioni
- cardiolipin binding Source: EcoCyc
- lipid-linked peptidoglycan transporter activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- glycolipid translocation Source: CACAO
- lipid-linked peptidoglycan transport Source: EcoCyc
- lipid translocation Source: GO_Central
- peptidoglycan biosynthetic process Source: EcoCyc
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G6561-MONOMER MetaCyc:G6561-MONOMER |
UniPathwayi | UPA00219 |
Protein family/group databases
TCDBi | 2.A.66.4.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Lipid II flippase MurJCuratedAlternative name(s): Peptidoglycan biosynthesis protein MurJCurated |
Gene namesi | Name:murJ1 PublicationUniRule annotation Synonyms:mviN, yceN Ordered Locus Names:b1069, JW1056 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation2 Publications; Multi-pass membrane protein UniRule annotation2 Publications
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- division septum Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 5 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 6 – 32 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 33 – 40 | PeriplasmicSequence analysis | 8 | |
Transmembranei | 41 – 69 | HelicalSequence analysisAdd BLAST | 29 | |
Topological domaini | 70 – 82 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 83 – 109 | HelicalSequence analysisAdd BLAST | 27 | |
Topological domaini | 110 – 123 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 124 – 154 | HelicalSequence analysisAdd BLAST | 31 | |
Topological domaini | 155 – 156 | CytoplasmicSequence analysis | 2 | |
Transmembranei | 157 – 178 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 179 – 185 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 186 – 207 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 208 – 223 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 224 – 253 | HelicalSequence analysisAdd BLAST | 30 | |
Topological domaini | 254 – 263 | PeriplasmicSequence analysis | 10 | |
Transmembranei | 264 – 292 | HelicalSequence analysisAdd BLAST | 29 | |
Topological domaini | 293 – 302 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 303 – 337 | HelicalSequence analysisAdd BLAST | 35 | |
Topological domaini | 338 – 346 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 347 – 374 | HelicalSequence analysisAdd BLAST | 28 | |
Topological domaini | 375 – 379 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 380 – 402 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 403 – 409 | PeriplasmicSequence analysis | 7 | |
Transmembranei | 410 – 429 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 430 – 443 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 444 – 463 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 464 – 477 | PeriplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 478 – 499 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 500 – 511 | CytoplasmicSequence analysisAdd BLAST | 12 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 18 | R → A or C: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 24 | R → A or C: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 39 | D → A or C: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 52 | R → A: Decrease in activity. 1 Publication | 1 | |
Mutagenesisi | 52 | R → C: Lack of activity. 1 Publication | 1 | |
Mutagenesisi | 270 | R → A or C: Lack of activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000182006 | 1 – 511 | Lipid II flippase MurJAdd BLAST | 511 |
Proteomic databases
PaxDbi | P0AF16 |
PRIDEi | P0AF16 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260079, 316 interactors |
IntActi | P0AF16, 1 interactor |
STRINGi | 511145.b1069 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AF16 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0728, Bacteria |
HOGENOMi | CLU_006797_5_3_6 |
InParanoidi | P0AF16 |
PhylomeDBi | P0AF16 |
Family and domain databases
CDDi | cd13123, MATE_MurJ_like, 1 hit |
HAMAPi | MF_02078, MurJ_MviN, 1 hit |
InterProi | View protein in InterPro IPR004268, MurJ |
Pfami | View protein in Pfam PF03023, MurJ, 1 hit |
PIRSFi | PIRSF002869, MviN, 1 hit |
PRINTSi | PR01806, VIRFACTRMVIN |
TIGRFAMsi | TIGR01695, murJ_mviN, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AF16-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLLKSLAAV SSMTMFSRVL GFARDAIVAR IFGAGMATDA FFVAFKLPNL
60 70 80 90 100
LRRIFAEGAF SQAFVPILAE YKSKQGEDAT RVFVSYVSGL LTLALAVVTV
110 120 130 140 150
AGMLAAPWVI MVTAPGFADT ADKFALTSQL LKITFPYILL ISLASLVGAI
160 170 180 190 200
LNTWNRFSIP AFAPTLLNIS MIGFALFAAP YFNPPVLALA WAVTVGGVLQ
210 220 230 240 250
LVYQLPHLKK IGMLVLPRIN FHDAGAMRVV KQMGPAILGV SVSQISLIIN
260 270 280 290 300
TIFASFLASG SVSWMYYADR LMEFPSGVLG VALGTILLPS LSKSFASGNH
310 320 330 340 350
DEYNRLMDWG LRLCFLLALP SAVALGILSG PLTVSLFQYG KFTAFDALMT
360 370 380 390 400
QRALIAYSVG LIGLIVVKVL APGFYSRQDI KTPVKIAIVT LILTQLMNLA
410 420 430 440 450
FIGPLKHAGL SLSIGLAACL NASLLYWQLR KQKIFTPQPG WMAFLLRLVV
460 470 480 490 500
AVLVMSGVLL GMLHIMPEWS LGTMPWRLLR LMAVVLAGIA AYFAALAVLG
510
FKVKEFARRT V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74153.1 AP009048 Genomic DNA Translation: BAA35877.1 |
PIRi | B64850 |
RefSeqi | NP_415587.1, NC_000913.3 WP_001050683.1, NZ_SSZK01000053.1 |
Genome annotation databases
EnsemblBacteriai | AAC74153; AAC74153; b1069 BAA35877; BAA35877; BAA35877 |
GeneIDi | 945487 |
KEGGi | ecj:JW1056 eco:b1069 |
PATRICi | fig|1411691.4.peg.1199 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74153.1 AP009048 Genomic DNA Translation: BAA35877.1 |
PIRi | B64850 |
RefSeqi | NP_415587.1, NC_000913.3 WP_001050683.1, NZ_SSZK01000053.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6CC4 | X-ray | 3.50 | A | 4-511 | [»] | |
SMRi | P0AF16 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260079, 316 interactors |
IntActi | P0AF16, 1 interactor |
STRINGi | 511145.b1069 |
Protein family/group databases
TCDBi | 2.A.66.4.3, the multidrug/oligosaccharidyl-lipid/polysaccharide (mop) flippase superfamily |
Proteomic databases
PaxDbi | P0AF16 |
PRIDEi | P0AF16 |
Genome annotation databases
EnsemblBacteriai | AAC74153; AAC74153; b1069 BAA35877; BAA35877; BAA35877 |
GeneIDi | 945487 |
KEGGi | ecj:JW1056 eco:b1069 |
PATRICi | fig|1411691.4.peg.1199 |
Organism-specific databases
EchoBASEi | EB3639 |
Phylogenomic databases
eggNOGi | COG0728, Bacteria |
HOGENOMi | CLU_006797_5_3_6 |
InParanoidi | P0AF16 |
PhylomeDBi | P0AF16 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:G6561-MONOMER MetaCyc:G6561-MONOMER |
Miscellaneous databases
PROi | PR:P0AF16 |
Family and domain databases
CDDi | cd13123, MATE_MurJ_like, 1 hit |
HAMAPi | MF_02078, MurJ_MviN, 1 hit |
InterProi | View protein in InterPro IPR004268, MurJ |
Pfami | View protein in Pfam PF03023, MurJ, 1 hit |
PIRSFi | PIRSF002869, MviN, 1 hit |
PRINTSi | PR01806, VIRFACTRMVIN |
TIGRFAMsi | TIGR01695, murJ_mviN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MURJ_ECOLI | |
Accessioni | P0AF16Primary (citable) accession number: P0AF16 Secondary accession number(s): P75932 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families