motB

Functionⁱ

MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication

GO - Biological processⁱ

archaeal or bacterial-type flagellum-dependent cell motility Source: UniProtKB-KW
chemotaxis Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Chemotaxis, Flagellar rotation

Biological process

Chemotaxis, Flagellar rotation

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN

BioCycⁱ

EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN

Protein family/group databases

TCDBⁱ	1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

TCDBⁱ

1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Motility protein B Alternative name(s): Chemotaxis protein MotB
Gene namesⁱ	Name:motB Ordered Locus Names:b1889, JW1878
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG10602 motB

EcoGeneⁱ

EG10602 motB

Subcellular locationⁱ

Cell inner membrane
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Bacterial motility: membrane topology of the Escherichia coli MotB protein."
  Chun S.Y., Parkinson J.S.
  Science 239:276-278(1988) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY, SUGGESTION OF ROLE AS A CELL-WALL ANCHOR.
; Single-pass type II membrane protein
1 Publication
Manual assertion based on experiment inⁱ
- Ref.6
  "Bacterial motility: membrane topology of the Escherichia coli MotB protein."
  Chun S.Y., Parkinson J.S.
  Science 239:276-278(1988) [PubMed] [Europe PMC] [Abstract]
  Cited for: SUBCELLULAR LOCATION, TOPOLOGY, SUGGESTION OF ROLE AS A CELL-WALL ANCHOR.

Note:

The OmpA-like domain probably functions to anchor the complex to the cell wall.

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 27	Cytoplasmic1 PublicationAdd BLAST	27
Transmembraneⁱ	28 – 49	Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST	22
Topological domainⁱ	50 – 308	Periplasmic1 PublicationAdd BLAST	259

GO - Cellular componentⁱ

bacterial-type flagellum stator complex
Source: CACAO
Inferred from direct assayⁱ
- 14705928
integral component of membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 2193926
plasma membrane
Source: EcoliWiki
Inferred from direct assayⁱ
- 2193926

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	31	A → T: Complete loss of motility.	1
Mutagenesisⁱ	32	D → N: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	39	A → V: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	159	P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication	1
Mutagenesisⁱ	164	G → D: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	196	T → I: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	197	D → N: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	205	E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication	1
Mutagenesisⁱ	214	S → F: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	217	R → W: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	222	R → H: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	240	G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication	1
Mutagenesisⁱ	242	A → T or V: Complete loss of motility. 1 Publication	1
Mutagenesisⁱ	258	R → C or H: Complete loss of motility. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000189585	1 – 308	Motility protein BAdd BLAST		308

Proteomic databases

PaxDbⁱ	P0AF06
PRIDEⁱ	P0AF06

Interactionⁱ

Subunit structureⁱ

Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.

Protein-protein interaction databases

BioGridⁱ	4261035, 417 interactors
Database of interacting proteins More...DIPⁱ	DIP-47996N
IntActⁱ	P0AF06, 4 interactors
STRINGⁱ	316385.ECDH10B_2030

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P0AF06
SMRⁱ	P0AF06
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	148 – 268	OmpA-likePROSITE-ProRule annotationAdd BLAST		121

Sequence similaritiesⁱ

Belongs to the MotB family.Curated

Keywords - Domainⁱ

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG4105ER2 Bacteria COG1360 LUCA
HOGENOMⁱ	HOG000254689
InParanoidⁱ	P0AF06
KEGG Orthology (KO) More...KOⁱ	K02557
PhylomeDBⁱ	P0AF06

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07185 OmpA_C-like, 1 hit
Gene3Dⁱ	3.30.1330.60, 1 hit
InterProⁱ	View protein in InterPro IPR025713 MotB_N_dom IPR006665 OmpA-like IPR036737 OmpA-like_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF13677 MotB_plug, 1 hit PF00691 OmpA, 1 hit
SUPFAMⁱ	SSF103088 SSF103088, 1 hit
PROSITEⁱ	View protein in PROSITE PS51123 OMPA_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P0AF06-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS 
        60         70         80         90        100
SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ 
       110        120        130        140        150
PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI 
       160        170        180        190        200
QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP 
       210        220        230        240        250
YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR 
       260        270        280        290        300
GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP 

TMPSAEPR

Length:308

Mass (Da):34,186

Last modified:December 20, 2005 - v1

Checksum:ⁱ2F20C551C44BD6E3

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01652 Genomic DNA Translation: AAA24178.1 U00096 Genomic DNA Translation: AAC74959.1 AP009048 Genomic DNA Translation: BAA15710.1 M34669 Genomic DNA Translation: AAA23572.1
PIRⁱ	A64952 QRECMB
NCBI Reference Sequences More...RefSeqⁱ	NP_416403.1, NC_000913.3 WP_000795630.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74959; AAC74959; b1889 BAA15710; BAA15710; BAA15710
GeneIDⁱ	946402
KEGGⁱ	ecj:JW1878 eco:b1889
PATRICⁱ	fig\|1411691.4.peg.358

Protein	Similar proteins		Species	Score	Length	Source
P0AF06	Motility protein B		ECO57		308	UniRef100_P0AF07
Motility protein B		Shigella sp.		308
Chemotaxis protein MotB		KLEPN		308
Flagellar motor protein MotB		Escherichia coli D6-113.11		308
Motility protein B		Escherichia coli M8		308
+95

Protein	Similar proteins		Species	Score	Length	Source
P0AF06	Motility protein B		ECO57		308	UniRef90_P0AF07
Motility protein B		Shigella sp.		308
Chemotaxis protein MotB		KLEPN		308
Flagellar motor protein MotB		Escherichia coli D6-113.11		308
Motility protein B		Escherichia coli M8		308
+103

Protein	Similar proteins		Species	Score	Length	Source
P0AF06	Motility protein B		ECO57		308	UniRef50_P0AF07
Motility protein B		Shigella sp.		308
Chemotaxis protein MotB		KLEPN		308
Flagellar motor protein MotB		Escherichia coli D6-113.11		308
Motility protein B		Escherichia coli M8		308
+103

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	J01652 Genomic DNA Translation: AAA24178.1 U00096 Genomic DNA Translation: AAC74959.1 AP009048 Genomic DNA Translation: BAA15710.1 M34669 Genomic DNA Translation: AAA23572.1
PIRⁱ	A64952 QRECMB
RefSeqⁱ	NP_416403.1, NC_000913.3 WP_000795630.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P0AF06
SMRⁱ	P0AF06
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4261035, 417 interactors
DIPⁱ	DIP-47996N
IntActⁱ	P0AF06, 4 interactors
STRINGⁱ	316385.ECDH10B_2030

Protein family/group databases

TCDBⁱ	1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

TCDBⁱ

1.A.30.1.1 the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily

Proteomic databases

PaxDbⁱ	P0AF06
PRIDEⁱ	P0AF06

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74959; AAC74959; b1889 BAA15710; BAA15710; BAA15710
GeneIDⁱ	946402
KEGGⁱ	ecj:JW1878 eco:b1889
PATRICⁱ	fig\|1411691.4.peg.358

Organism-specific databases

EchoBASEⁱ	EB0597
EcoGeneⁱ	EG10602 motB

Phylogenomic databases

eggNOGⁱ	ENOG4105ER2 Bacteria COG1360 LUCA
HOGENOMⁱ	HOG000254689
InParanoidⁱ	P0AF06
KOⁱ	K02557
PhylomeDBⁱ	P0AF06

Enzyme and pathway databases

BioCycⁱ	EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN

BioCycⁱ

EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P0AF06

PROⁱ

PR:P0AF06

Family and domain databases

CDDⁱ	cd07185 OmpA_C-like, 1 hit
Gene3Dⁱ	3.30.1330.60, 1 hit
InterProⁱ	View protein in InterPro IPR025713 MotB_N_dom IPR006665 OmpA-like IPR036737 OmpA-like_sf
Pfamⁱ	View protein in Pfam PF13677 MotB_plug, 1 hit PF00691 OmpA, 1 hit
SUPFAMⁱ	SSF103088 SSF103088, 1 hit
PROSITEⁱ	View protein in PROSITE PS51123 OMPA_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MOTB_ECOLI
Accessionⁱ	P0AF06Primary (citable) accession number: P0AF06 Secondary accession number(s): P09349
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
	Last sequence update:	December 20, 2005
	Last modified:	November 7, 2018
	This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P0AF06 (MOTB_ECOLI)

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Motility protein B

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

GO - Cellular componenti

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ