UniProtKB - P0AF06 (MOTB_ECOLI)
Protein
Motility protein B
Gene
motB
Organism
Escherichia coli (strain K12)
Status
Functioni
MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Probably a linker that fastens the torque-generating machinery to the cell wall. Overexpression of this protein with MotA improves motility in a pdeH disruption, (a c-di-GMP phosphodiesterase) suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein YcgR and the flagellar stator.1 Publication
GO - Biological processi
- archaeal or bacterial-type flagellum-dependent cell motility Source: UniProtKB-KW
- chemotaxis Source: UniProtKB-KW
Keywordsi
Biological process | Chemotaxis, Flagellar rotation |
Enzyme and pathway databases
BioCyci | EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN |
Protein family/group databases
TCDBi | 1.A.30.1.1, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Motility protein BAlternative name(s): Chemotaxis protein MotB |
Gene namesi | Name:motB Ordered Locus Names:b1889, JW1878 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Single-pass type II membrane protein 1 Publication Note: The OmpA-like domain probably functions to anchor the complex to the cell wall.
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 27 | Cytoplasmic1 PublicationAdd BLAST | 27 | |
Transmembranei | 28 – 49 | Helical; Signal-anchor for type II membrane proteinCuratedAdd BLAST | 22 | |
Topological domaini | 50 – 308 | Periplasmic1 PublicationAdd BLAST | 259 |
GO - Cellular componenti
- integral component of membrane Source: EcoliWiki
- plasma membrane Source: EcoliWiki
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | A → T: Complete loss of motility. | 1 | |
Mutagenesisi | 32 | D → N: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 39 | A → V: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 159 | P → I: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication | 1 | |
Mutagenesisi | 164 | G → D: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 196 | T → I: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 197 | D → N: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 205 | E → K: Decreased motility, subnormal torque, tethered strains rotate very slowly, maybe reduced affinity for the motor. 1 Publication | 1 | |
Mutagenesisi | 214 | S → F: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 217 | R → W: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 222 | R → H: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 240 | G → D: Decreased motility, subnormal torque, tethered strains rotate very slowly. 1 Publication | 1 | |
Mutagenesisi | 242 | A → T or V: Complete loss of motility. 1 Publication | 1 | |
Mutagenesisi | 258 | R → C or H: Complete loss of motility. 1 Publication | 1 |
Miscellaneous databases
PHI-basei | PHI:6534 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000189585 | 1 – 308 | Motility protein BAdd BLAST | 308 |
Proteomic databases
PaxDbi | P0AF06 |
PRIDEi | P0AF06 |
Interactioni
Subunit structurei
Each stator complex is composed of 4 MotA and 2 MotB subunits; in E.coli 11 to 12 stator complexes can be involved in flagellar rotation. 2 A subunits and 1 B subunit are thought to form a single ion channel, so that each stator complex contains two channels.
Binary interactionsi
P0AF06
With | #Exp. | IntAct |
---|---|---|
motA [P09348] | 2 | EBI-1117399,EBI-557926 |
Protein-protein interaction databases
BioGRIDi | 4261035, 417 interactors |
ComplexPortali | CPX-5884, Flagellar motor stator complex |
DIPi | DIP-47996N |
IntActi | P0AF06, 5 interactors |
STRINGi | 511145.b1889 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 148 – 268 | OmpA-likePROSITE-ProRule annotationAdd BLAST | 121 |
Sequence similaritiesi
Belongs to the MotB family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1360, Bacteria |
HOGENOMi | CLU_016890_3_0_6 |
InParanoidi | P0AF06 |
PhylomeDBi | P0AF06 |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
InterProi | View protein in InterPro IPR025713, MotB_N_dom IPR006665, OmpA-like IPR036737, OmpA-like_sf |
Pfami | View protein in Pfam PF13677, MotB_plug, 1 hit PF00691, OmpA, 1 hit |
SUPFAMi | SSF103088, SSF103088, 1 hit |
PROSITEi | View protein in PROSITE PS51123, OMPA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AF06-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNQAHPIIV VKRRKAKSHG AAHGSWKIAY ADFMTAMMAF FLVMWLISIS
60 70 80 90 100
SPKELIQIAE YFRTPLATAV TGGDRISNSE SPIPGGGDDY TQSQGEVNKQ
110 120 130 140 150
PNIEELKKRM EQSRLRKLRG DLDQLIESDP KLRALRPHLK IDLVQEGLRI
160 170 180 190 200
QIIDSQNRPM FRTGSADVEP YMRDILRAIA PVLNGIPNRI SLSGHTDDFP
210 220 230 240 250
YASGEKGYSN WELSADRANA SRRELMVGGL DSGKVLRVVG MAATMRLSDR
260 270 280 290 300
GPDDAVNRRI SLLVLNKQAE QAILHENAES QNEPVSALEK PEVAPQVSVP
TMPSAEPR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01652 Genomic DNA Translation: AAA24178.1 U00096 Genomic DNA Translation: AAC74959.1 AP009048 Genomic DNA Translation: BAA15710.1 M34669 Genomic DNA Translation: AAA23572.1 |
PIRi | A64952, QRECMB |
RefSeqi | NP_416403.1, NC_000913.3 WP_000795630.1, NZ_STEB01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC74959; AAC74959; b1889 BAA15710; BAA15710; BAA15710 |
GeneIDi | 57731288 946402 |
KEGGi | ecj:JW1878 eco:b1889 |
PATRICi | fig|1411691.4.peg.358 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01652 Genomic DNA Translation: AAA24178.1 U00096 Genomic DNA Translation: AAC74959.1 AP009048 Genomic DNA Translation: BAA15710.1 M34669 Genomic DNA Translation: AAA23572.1 |
PIRi | A64952, QRECMB |
RefSeqi | NP_416403.1, NC_000913.3 WP_000795630.1, NZ_STEB01000026.1 |
3D structure databases
SMRi | P0AF06 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261035, 417 interactors |
ComplexPortali | CPX-5884, Flagellar motor stator complex |
DIPi | DIP-47996N |
IntActi | P0AF06, 5 interactors |
STRINGi | 511145.b1889 |
Protein family/group databases
TCDBi | 1.A.30.1.1, the h(+)- or na(+)-translocating bacterial flagellar motor/exbbd outer membrane transport energizer (mot/exb) superfamily |
Proteomic databases
PaxDbi | P0AF06 |
PRIDEi | P0AF06 |
Genome annotation databases
EnsemblBacteriai | AAC74959; AAC74959; b1889 BAA15710; BAA15710; BAA15710 |
GeneIDi | 57731288 946402 |
KEGGi | ecj:JW1878 eco:b1889 |
PATRICi | fig|1411691.4.peg.358 |
Organism-specific databases
EchoBASEi | EB0597 |
Phylogenomic databases
eggNOGi | COG1360, Bacteria |
HOGENOMi | CLU_016890_3_0_6 |
InParanoidi | P0AF06 |
PhylomeDBi | P0AF06 |
Enzyme and pathway databases
BioCyci | EcoCyc:MOTB-FLAGELLAR-MOTOR-STATOR-PROTEIN |
Miscellaneous databases
PHI-basei | PHI:6534 |
PROi | PR:P0AF06 |
Family and domain databases
CDDi | cd07185, OmpA_C-like, 1 hit |
Gene3Di | 3.30.1330.60, 1 hit |
InterProi | View protein in InterPro IPR025713, MotB_N_dom IPR006665, OmpA-like IPR036737, OmpA-like_sf |
Pfami | View protein in Pfam PF13677, MotB_plug, 1 hit PF00691, OmpA, 1 hit |
SUPFAMi | SSF103088, SSF103088, 1 hit |
PROSITEi | View protein in PROSITE PS51123, OMPA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MOTB_ECOLI | |
Accessioni | P0AF06Primary (citable) accession number: P0AF06 Secondary accession number(s): P09349 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 10, 2021 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families