UniProtKB - P0AEZ7 (MLTD_ECOLI)
Protein
Membrane-bound lytic murein transglycosylase D
Gene
mltD
Organism
Escherichia coli (strain K12)
Status
Functioni
Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division (By similarity).By similarity
Caution
Was originally thought to be involved in hexaheme nitrite reductase (cytochrome c552) expression.1 Publication
Catalytic activityi
- Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue. EC:4.2.2.n1
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 125 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- lyase activity Source: UniProtKB-KW
- lytic endotransglycosylase activity Source: EcoCyc
- lytic transglycosylase activity Source: EcoCyc
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan metabolic process Source: InterPro
- positive regulation of oxidoreductase activity Source: EcoliWiki
Keywordsi
Molecular function | Lyase |
Biological process | Cell wall biogenesis/degradation |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10246-MONOMER MetaCyc:EG10246-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Membrane-bound lytic murein transglycosylase D (EC:4.2.2.n1)Alternative name(s): Murein hydrolase D Regulatory protein DniR |
Gene namesi | Name:mltD Synonyms:dniR, yafG Ordered Locus Names:b0211, JW5018 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell membrane Curated; Lipid-anchor Curated
GO - Cellular componenti
- membrane Source: EcoliWiki
- plasma membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 15 | PROSITE-ProRule annotationAdd BLAST | 15 | |
ChainiPRO_0000032801 | 16 – 452 | Membrane-bound lytic murein transglycosylase DAdd BLAST | 437 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 16 | N-palmitoyl cysteinePROSITE-ProRule annotation | 1 | |
Lipidationi | 16 | S-diacylglycerol cysteinePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
PaxDbi | P0AEZ7 |
PRIDEi | P0AEZ7 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261468, 281 interactors |
DIPi | DIP-48010N |
IntActi | P0AEZ7, 5 interactors |
STRINGi | 511145.b0211 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEZ7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEZ7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 341 – 384 | LysM 1PROSITE-ProRule annotationAdd BLAST | 44 | |
Domaini | 400 – 448 | LysM 2PROSITE-ProRule annotationAdd BLAST | 49 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 113 – 198 | Slt-type domainAdd BLAST | 86 |
Domaini
LysM domains are thought to be involved in peptidoglycan binding.
Sequence similaritiesi
Belongs to the transglycosylase Slt family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | COG0741, Bacteria COG1388, Bacteria |
HOGENOMi | CLU_009520_1_4_6 |
InParanoidi | P0AEZ7 |
PhylomeDBi | P0AEZ7 |
Family and domain databases
CDDi | cd00118, LysM, 2 hits |
Gene3Di | 3.10.350.10, 2 hits |
InterProi | View protein in InterPro IPR018392, LysM_dom IPR036779, LysM_dom_sf IPR023346, Lysozyme-like_dom_sf IPR000189, Transglyc_AS IPR008258, Transglycosylase_SLT_dom_1 |
Pfami | View protein in Pfam PF01476, LysM, 2 hits PF01464, SLT, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 2 hits |
SUPFAMi | SSF53955, SSF53955, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 2 hits PS51257, PROKAR_LIPOPROTEIN, 1 hit PS00922, TRANSGLYCOSYLASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEZ7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKAKAILLAS VLLVGCQSTG NVQQHAQSLS AAGQGEAAKF TSQARWMDDG
60 70 80 90 100
TSIAPDGDLW AFIGDELKMG IPENDRIREQ KQKYLRNKSY LHDVTLRAEP
110 120 130 140 150
YMYWIAGQVK KRNMPMELVL LPIVESAFDP HATSGANAAG IWQIIPSTGR
160 170 180 190 200
NYGLKQTRNY DARRDVVAST TAALNMMQRL NKMFDGDWLL TVAAYNSGEG
210 220 230 240 250
RVMKAIKTNK ARGKSTDFWS LPLPQETKQY VPKMLALSDI LKNSKRYGVR
260 270 280 290 300
LPTTDESRAL ARVHLSSPVE MAKVADMAGI SVSKLKTFNA GVKGSTLGAS
310 320 330 340 350
GPQYVMVPKK HADQLRESLA SGEIAAVQST LVADNTPLNS RVYTVRSGDT
360 370 380 390 400
LSSIASRLGV STKDLQQWNK LRGSKLKPGQ SLTIGAGSSA QRLANNSDSI
410 420 430 440 450
TYRVRKGDSL SSIAKRHGVN IKDVMRWNSD TANLQPGDKL TLFVKNNNMP
DS
Sequence cautioni
The sequence CAA43144 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 69 – 72 | MGIP → DGNS (PubMed:1663890).Curated | 4 | |
Sequence conflicti | 217 | D → A (PubMed:1663890).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08633.1 U00096 Genomic DNA Translation: AAC73316.1 AP009048 Genomic DNA Translation: BAA77882.2 X60739 Genomic DNA Translation: CAA43144.1 Frameshift. |
PIRi | E64745 |
RefSeqi | NP_414747.1, NC_000913.3 WP_000644685.1, NZ_STEB01000020.1 |
Genome annotation databases
EnsemblBacteriai | AAC73316; AAC73316; b0211 BAA77882; BAA77882; BAA77882 |
GeneIDi | 49585972 945694 |
KEGGi | ecj:JW5018 eco:b0211 |
PATRICi | fig|511145.12.peg.213 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U70214 Genomic DNA Translation: AAB08633.1 U00096 Genomic DNA Translation: AAC73316.1 AP009048 Genomic DNA Translation: BAA77882.2 X60739 Genomic DNA Translation: CAA43144.1 Frameshift. |
PIRi | E64745 |
RefSeqi | NP_414747.1, NC_000913.3 WP_000644685.1, NZ_STEB01000020.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1E0G | NMR | - | A | 398-445 | [»] | |
SMRi | P0AEZ7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261468, 281 interactors |
DIPi | DIP-48010N |
IntActi | P0AEZ7, 5 interactors |
STRINGi | 511145.b0211 |
Proteomic databases
PaxDbi | P0AEZ7 |
PRIDEi | P0AEZ7 |
Genome annotation databases
EnsemblBacteriai | AAC73316; AAC73316; b0211 BAA77882; BAA77882; BAA77882 |
GeneIDi | 49585972 945694 |
KEGGi | ecj:JW5018 eco:b0211 |
PATRICi | fig|511145.12.peg.213 |
Organism-specific databases
EchoBASEi | EB0242 |
Phylogenomic databases
eggNOGi | COG0741, Bacteria COG1388, Bacteria |
HOGENOMi | CLU_009520_1_4_6 |
InParanoidi | P0AEZ7 |
PhylomeDBi | P0AEZ7 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10246-MONOMER MetaCyc:EG10246-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AEZ7 |
PROi | PR:P0AEZ7 |
Family and domain databases
CDDi | cd00118, LysM, 2 hits |
Gene3Di | 3.10.350.10, 2 hits |
InterProi | View protein in InterPro IPR018392, LysM_dom IPR036779, LysM_dom_sf IPR023346, Lysozyme-like_dom_sf IPR000189, Transglyc_AS IPR008258, Transglycosylase_SLT_dom_1 |
Pfami | View protein in Pfam PF01476, LysM, 2 hits PF01464, SLT, 1 hit |
SMARTi | View protein in SMART SM00257, LysM, 2 hits |
SUPFAMi | SSF53955, SSF53955, 1 hit |
PROSITEi | View protein in PROSITE PS51782, LYSM, 2 hits PS51257, PROKAR_LIPOPROTEIN, 1 hit PS00922, TRANSGLYCOSYLASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MLTD_ECOLI | |
Accessioni | P0AEZ7Primary (citable) accession number: P0AEZ7 Secondary accession number(s): P23931, P32982, P77350 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 127 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families