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Protein

Maltose/maltodextrin-binding periplasmic protein

Gene

malE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.4 Publications

Kineticsi

  1. KM=0.9 µM for maltose1 Publication
  2. KM=2 µM for maltotriose1 Publication

    GO - Molecular functioni

    • carbohydrate transmembrane transporter activity Source: InterPro
    • maltose binding Source: EcoCyc
    • transporter activity Source: EcoliWiki

    GO - Biological processi

    • carbohydrate transport Source: EcoliWiki
    • cell chemotaxis Source: EcoCyc
    • cellular response to DNA damage stimulus Source: EcoliWiki
    • detection of maltose stimulus Source: EcoCyc
    • maltodextrin transport Source: EcoCyc
    • maltose transport Source: EcoCyc

    Keywordsi

    Biological processSugar transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:MALE-MONOMER
    MetaCyc:MALE-MONOMER

    Protein family/group databases

    TCDBi3.A.1.1.1 the atp-binding cassette (abc) superfamily

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Maltose/maltodextrin-binding periplasmic proteinCurated
    Alternative name(s):
    MMBPCurated
    Maltodextrin-binding proteinCurated
    Maltose-binding protein1 Publication
    Short name:
    MBP1 Publication
    Gene namesi
    Name:malE1 Publication
    Ordered Locus Names:b4034, JW3994
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10554 malE

    Subcellular locationi

    • Periplasm 1 Publication

    GO - Cellular componenti

    • ATP-binding cassette (ABC) transporter complex Source: EcoliWiki
    • ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing Source: EcoliWiki
    • outer membrane-bounded periplasmic space Source: EcoCyc
    • periplasmic space Source: EcoliWiki

    Keywords - Cellular componenti

    Periplasm

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB02379 Beta-D-Glucose
    DB03995 Cyclo-Hepta-Amylose
    DB01914 D-glucose
    DB03323 Maltose

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 262 PublicationsAdd BLAST26
    ChainiPRO_000003169427 – 396Maltose/maltodextrin-binding periplasmic proteinAdd BLAST370

    Proteomic databases

    PaxDbiP0AEX9
    PRIDEiP0AEX9

    2D gel databases

    SWISS-2DPAGEiP0AEX9

    Expressioni

    Inductioni

    Induced by maltose and the transcriptional regulator MalT (PubMed:4215651). Permanently repressed by cold shock in a PNPase-dependent fashion (PubMed:14527658).2 Publications

    Interactioni

    Subunit structurei

    The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE).2 Publications

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi4263065, 24 interactors
    852832, 1 interactor
    ComplexPortaliCPX-1932 Maltose transport complex
    DIPiDIP-31871N
    IntActiP0AEX9, 10 interactors
    MINTiP0AEX9
    STRINGi316385.ECDH10B_4223

    Structurei

    Secondary structure

    1396
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni28 – 31Combined sources4
    Beta strandi32 – 36Combined sources5
    Helixi39 – 41Combined sources3
    Helixi43 – 57Combined sources15
    Beta strandi60 – 64Combined sources5
    Helixi69 – 76Combined sources8
    Turni77 – 80Combined sources4
    Beta strandi84 – 89Combined sources6
    Helixi90 – 92Combined sources3
    Helixi93 – 98Combined sources6
    Beta strandi99 – 101Combined sources3
    Helixi109 – 112Combined sources4
    Helixi117 – 122Combined sources6
    Beta strandi123 – 125Combined sources3
    Beta strandi126 – 129Combined sources4
    Beta strandi131 – 137Combined sources7
    Beta strandi140 – 144Combined sources5
    Turni145 – 147Combined sources3
    Beta strandi148 – 150Combined sources3
    Beta strandi153 – 155Combined sources3
    Helixi158 – 166Combined sources9
    Turni167 – 169Combined sources3
    Beta strandi171 – 173Combined sources3
    Beta strandi177 – 179Combined sources3
    Helixi180 – 189Combined sources10
    Beta strandi193 – 198Combined sources6
    Beta strandi201 – 208Combined sources8
    Beta strandi209 – 211Combined sources3
    Helixi212 – 226Combined sources15
    Beta strandi227 – 230Combined sources4
    Helixi236 – 244Combined sources9
    Beta strandi248 – 253Combined sources6
    Helixi255 – 257Combined sources3
    Helixi258 – 263Combined sources6
    Beta strandi264 – 266Combined sources3
    Beta strandi268 – 271Combined sources4
    Beta strandi275 – 279Combined sources5
    Beta strandi284 – 293Combined sources10
    Beta strandi294 – 297Combined sources4
    Helixi299 – 308Combined sources10
    Turni309 – 311Combined sources3
    Helixi313 – 322Combined sources10
    Beta strandi323 – 325Combined sources3
    Beta strandi327 – 330Combined sources4
    Helixi331 – 337Combined sources7
    Beta strandi338 – 340Combined sources3
    Helixi341 – 352Combined sources12
    Beta strandi353 – 355Combined sources3
    Helixi362 – 377Combined sources16
    Beta strandi379 – 381Combined sources3
    Helixi383 – 392Combined sources10

    3D structure databases

    ProteinModelPortaliP0AEX9
    SMRiP0AEX9
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEX9

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4107FKK Bacteria
    COG2182 LUCA
    HOGENOMiHOG000055250
    InParanoidiP0AEX9
    KOiK10108
    OMAiWAHDWIG
    PhylomeDBiP0AEX9

    Family and domain databases

    InterProiView protein in InterPro
    IPR006060 Maltose/Cyclodextrin-bd
    IPR006059 SBP
    IPR006061 SBP_1_CS
    PfamiView protein in Pfam
    PF13416 SBP_bac_8, 1 hit
    PRINTSiPR00181 MALTOSEBP
    PROSITEiView protein in PROSITE
    PS01037 SBP_BACTERIAL_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AEX9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIKTGARIL ALSALTTMMF SASALAKIEE GKLVIWINGD KGYNGLAEVG
    60 70 80 90 100
    KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG
    110 120 130 140 150
    LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN
    160 170 180 190 200
    PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG
    210 220 230 240 250
    KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM
    260 270 280 290 300
    TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE
    310 320 330 340 350
    LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA
    360 370 380 390
    QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITK
    Length:396
    Mass (Da):43,388
    Last modified:July 21, 1986 - v1
    Checksum:iA4C1B3C1777ADE47
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti36W → A AA sequence (PubMed:9298646).Curated1
    Sequence conflicti46L → H in CAA23581 (PubMed:6283312).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    V00303 Genomic DNA Translation: CAA23581.1
    U00006 Genomic DNA Translation: AAC43128.1
    U00096 Genomic DNA Translation: AAC77004.1
    AP009048 Genomic DNA Translation: BAE78036.1
    J01648 Genomic DNA Translation: AAB59056.1
    M16181 Genomic DNA Translation: AAA24102.1
    M12635 Genomic DNA Translation: AAA24123.1
    M12647 Genomic DNA Translation: AAA24135.1
    M12650 Genomic DNA Translation: AAA24138.1
    M12653 Genomic DNA Translation: AAA24115.1
    PIRiA03428 JGECM
    RefSeqiNP_418458.1, NC_000913.3
    WP_000695387.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77004; AAC77004; b4034
    BAE78036; BAE78036; BAE78036
    GeneIDi948538
    KEGGiecj:JW3994
    eco:b4034
    PATRICifig|1411691.4.peg.2677

    Similar proteinsi

    Entry informationi

    Entry nameiMALE_ECOLI
    AccessioniPrimary (citable) accession number: P0AEX9
    Secondary accession number(s): P02928, Q2M6S0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: June 20, 2018
    This is version 139 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

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