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Protein

Maltose/maltodextrin-binding periplasmic protein

Gene

malE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex MalEFGK involved in maltose/maltodextrin import. Binds maltose and higher maltodextrins such as maltotriose.4 Publications

Kineticsi

  1. KM=0.9 µM for maltose1 Publication
  2. KM=2 µM for maltotriose1 Publication

    GO - Molecular functioni

    • carbohydrate transmembrane transporter activity Source: InterPro
    • maltose binding Source: EcoCyc
    • transporter activity Source: EcoliWiki

    GO - Biological processi

    • carbohydrate transport Source: EcoliWiki
    • cell chemotaxis Source: EcoCyc
    • cellular response to DNA damage stimulus Source: EcoliWiki
    • detection of maltose stimulus Source: EcoCyc
    • maltodextrin transport Source: EcoCyc
    • maltose transport Source: EcoCyc

    Keywordsi

    Biological processSugar transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:MALE-MONOMER
    MetaCyc:MALE-MONOMER

    Protein family/group databases

    TCDBi3.A.1.1.1 the atp-binding cassette (abc) superfamily

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Maltose/maltodextrin-binding periplasmic proteinCurated
    Alternative name(s):
    MMBPCurated
    Maltodextrin-binding proteinCurated
    Maltose-binding protein1 Publication
    Short name:
    MBP1 Publication
    Gene namesi
    Name:malE1 Publication
    Ordered Locus Names:b4034, JW3994
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10554 malE

    Subcellular locationi

    • Periplasm 1 Publication

    GO - Cellular componenti

    Keywords - Cellular componenti

    Periplasm

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB02379 Beta-D-Glucose
    DB03995 Cyclo-Hepta-Amylose
    DB01914 D-glucose
    DB03323 Maltose

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 262 PublicationsAdd BLAST26
    ChainiPRO_000003169427 – 396Maltose/maltodextrin-binding periplasmic proteinAdd BLAST370

    Proteomic databases

    PaxDbiP0AEX9
    PRIDEiP0AEX9

    2D gel databases

    SWISS-2DPAGEiP0AEX9

    Expressioni

    Inductioni

    Induced by maltose and the transcriptional regulator MalT (PubMed:4215651). Permanently repressed by cold shock in a PNPase-dependent fashion (PubMed:14527658).2 Publications

    Interactioni

    Subunit structurei

    The complex is composed of two ATP-binding proteins (MalK), two transmembrane proteins (MalG and MalF) and a solute-binding protein (MalE).2 Publications

    Binary interactionsi

    Protein-protein interaction databases

    BioGridi4263065, 24 interactors
    852832, 1 interactor
    ComplexPortaliCPX-1932 Maltose transport complex
    DIPiDIP-31871N
    IntActiP0AEX9, 10 interactors
    MINTiP0AEX9
    STRINGi316385.ECDH10B_4223

    Structurei

    Secondary structure

    1396
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP0AEX9
    SMRiP0AEX9
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEX9

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4107FKK Bacteria
    COG2182 LUCA
    HOGENOMiHOG000055250
    InParanoidiP0AEX9
    KOiK10108
    PhylomeDBiP0AEX9

    Family and domain databases

    InterProiView protein in InterPro
    IPR006060 Maltose/Cyclodextrin-bd
    IPR006059 SBP
    IPR006061 SBP_1_CS
    PfamiView protein in Pfam
    PF13416 SBP_bac_8, 1 hit
    PRINTSiPR00181 MALTOSEBP
    PROSITEiView protein in PROSITE
    PS01037 SBP_BACTERIAL_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0AEX9-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MKIKTGARIL ALSALTTMMF SASALAKIEE GKLVIWINGD KGYNGLAEVG
    60 70 80 90 100
    KKFEKDTGIK VTVEHPDKLE EKFPQVAATG DGPDIIFWAH DRFGGYAQSG
    110 120 130 140 150
    LLAEITPDKA FQDKLYPFTW DAVRYNGKLI AYPIAVEALS LIYNKDLLPN
    160 170 180 190 200
    PPKTWEEIPA LDKELKAKGK SALMFNLQEP YFTWPLIAAD GGYAFKYENG
    210 220 230 240 250
    KYDIKDVGVD NAGAKAGLTF LVDLIKNKHM NADTDYSIAE AAFNKGETAM
    260 270 280 290 300
    TINGPWAWSN IDTSKVNYGV TVLPTFKGQP SKPFVGVLSA GINAASPNKE
    310 320 330 340 350
    LAKEFLENYL LTDEGLEAVN KDKPLGAVAL KSYEEELAKD PRIAATMENA
    360 370 380 390
    QKGEIMPNIP QMSAFWYAVR TAVINAASGR QTVDEALKDA QTRITK
    Length:396
    Mass (Da):43,388
    Last modified:July 21, 1986 - v1
    Checksum:iA4C1B3C1777ADE47
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti36W → A AA sequence (PubMed:9298646).Curated1
    Sequence conflicti46L → H in CAA23581 (PubMed:6283312).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    V00303 Genomic DNA Translation: CAA23581.1
    U00006 Genomic DNA Translation: AAC43128.1
    U00096 Genomic DNA Translation: AAC77004.1
    AP009048 Genomic DNA Translation: BAE78036.1
    J01648 Genomic DNA Translation: AAB59056.1
    M16181 Genomic DNA Translation: AAA24102.1
    M12635 Genomic DNA Translation: AAA24123.1
    M12647 Genomic DNA Translation: AAA24135.1
    M12650 Genomic DNA Translation: AAA24138.1
    M12653 Genomic DNA Translation: AAA24115.1
    PIRiA03428 JGECM
    RefSeqiNP_418458.1, NC_000913.3
    WP_000695387.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC77004; AAC77004; b4034
    BAE78036; BAE78036; BAE78036
    GeneIDi948538
    KEGGiecj:JW3994
    eco:b4034
    PATRICifig|1411691.4.peg.2677

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    V00303 Genomic DNA Translation: CAA23581.1
    U00006 Genomic DNA Translation: AAC43128.1
    U00096 Genomic DNA Translation: AAC77004.1
    AP009048 Genomic DNA Translation: BAE78036.1
    J01648 Genomic DNA Translation: AAB59056.1
    M16181 Genomic DNA Translation: AAA24102.1
    M12635 Genomic DNA Translation: AAA24123.1
    M12647 Genomic DNA Translation: AAA24135.1
    M12650 Genomic DNA Translation: AAA24138.1
    M12653 Genomic DNA Translation: AAA24115.1
    PIRiA03428 JGECM
    RefSeqiNP_418458.1, NC_000913.3
    WP_000695387.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1A7LX-ray2.90A/B/C27-389[»]
    1ANFX-ray1.67A27-396[»]
    1DMBX-ray1.80A27-396[»]
    1EZ9X-ray1.90A/B27-396[»]
    1EZONMR-A29-396[»]
    1EZPNMR-A29-396[»]
    1FQAX-ray1.90A28-396[»]
    1FQBX-ray1.90A28-396[»]
    1FQCX-ray2.30A28-396[»]
    1FQDX-ray2.30A28-396[»]
    1HSJX-ray2.30A/B27-396[»]
    1IUDX-ray2.70A27-396[»]
    1JVXX-ray2.50A27-396[»]
    1JVYX-ray1.90A27-396[»]
    1JW4X-ray2.00A27-396[»]
    1JW5X-ray2.00A27-396[»]
    1LAXX-ray1.85A/C27-396[»]
    1LLSX-ray1.80A27-396[»]
    1MDPX-ray2.301/227-396[»]
    1MDQX-ray1.90A27-396[»]
    1MG1X-ray2.50A31-392[»]
    1MH3X-ray2.10A27-392[»]
    1MH4X-ray2.30A27-392[»]
    1MPBX-ray2.00A27-396[»]
    1MPCX-ray2.10A27-396[»]
    1MPDX-ray2.30A27-396[»]
    1N3WX-ray2.60A27-396[»]
    1N3XX-ray2.50A27-396[»]
    1NL5X-ray2.10A27-396[»]
    1NMUX-ray2.31A/C27-392[»]
    1OMPX-ray1.80A27-396[»]
    1PEBX-ray2.60A27-396[»]
    1R6ZX-ray2.80A/P/Z27-392[»]
    1SVXX-ray2.24B29-392[»]
    1T0KX-ray3.24A29-392[»]
    1Y4CX-ray1.90A27-392[»]
    1YTVX-ray1.80A/B27-392[»]
    1ZIUX-ray2.00A27-396[»]
    1ZJLX-ray2.00A27-396[»]
    1ZKBX-ray2.20A27-396[»]
    1ZMGX-ray2.50A27-396[»]
    2D21NMR-A27-396[»]
    2H25NMR-A27-396[»]
    2KLFNMR-A27-396[»]
    2MV0NMR-A27-396[»]
    2N44NMR-A27-396[»]
    2N45NMR-A27-396[»]
    2NVUX-ray2.80B25-392[»]
    2OBGX-ray2.35A31-396[»]
    2OK2X-ray2.00A/B28-392[»]
    2R6GX-ray2.80E27-396[»]
    2V93NMR-A27-396[»]
    2VGQX-ray2.10A27-392[»]
    2XZ3X-ray1.95A27-392[»]
    2ZXTX-ray3.00A27-392[»]
    3A3CX-ray2.50A29-392[»]
    3C4MX-ray1.95A/B26-392[»]
    3CSBX-ray2.00A31-396[»]
    3CSGX-ray1.80A31-396[»]
    3D4CX-ray2.90A27-393[»]
    3D4GX-ray2.30A/B/C/D/E/F/G/H27-393[»]
    3DM0X-ray2.40A27-387[»]
    3EF7X-ray3.10A/B27-393[»]
    3EHSX-ray2.76A26-392[»]
    3EHTX-ray3.40A26-392[»]
    3EHUX-ray1.96A/B26-392[»]
    3F5FX-ray2.65A27-387[»]
    3G7VX-ray1.86A/B/C/D27-392[»]
    3G7WX-ray1.75A27-392[»]
    3H3GX-ray1.94A26-392[»]
    3H4ZX-ray2.35A/B/C27-392[»]
    3HPIX-ray2.00A/B27-396[»]
    3HSTX-ray2.25A/C27-392[»]
    3IO4X-ray3.63A/B/C27-384[»]
    3IO6X-ray3.70A/B/C27-384[»]
    3IORX-ray3.60A/B/C27-384[»]
    3IOTX-ray3.50A/B/C27-384[»]
    3IOUX-ray3.70A/B/C27-384[»]
    3IOVX-ray3.70A/B/C27-384[»]
    3IOWX-ray3.50A/B/C27-384[»]
    3J9Pelectron microscopy4.24A/B/C/D27-392[»]
    3KJTX-ray1.50A27-396[»]
    3L2JX-ray3.24A/B29-392[»]
    3LBSX-ray2.15A/B29-390[»]
    3LC8X-ray2.00A/B29-390[»]
    3MBPX-ray1.70A27-396[»]
    3MP1X-ray2.60A27-387[»]
    3MP6X-ray1.48A27-387[»]
    3MP8X-ray1.92A27-387[»]
    3MQ9X-ray2.80A/B/C/D/E/F/G/H27-395[»]
    3N94X-ray1.80A26-392[»]
    3O3UX-ray1.50N28-384[»]
    3OAIX-ray2.10A/B27-392[»]
    3OB4X-ray2.71A27-393[»]
    3OSQX-ray1.90A27-396[»]
    3OSRX-ray2.00A/B27-396[»]
    3PGFX-ray2.10A27-393[»]
    3PUVX-ray2.40E27-396[»]
    3PUWX-ray2.30E27-396[»]
    3PUXX-ray2.30E27-396[»]
    3PUYX-ray3.10E27-396[»]
    3PUZX-ray2.90E27-396[»]
    3PV0X-ray3.10E27-396[»]
    3PY7X-ray2.29A27-392[»]
    3Q25X-ray1.90A27-392[»]
    3Q26X-ray1.54A27-392[»]
    3Q27X-ray1.30A27-392[»]
    3Q28X-ray1.60A27-392[»]
    3Q29X-ray2.30A/C27-392[»]
    3RLFX-ray2.20E27-396[»]
    3RUMX-ray1.85A27-392[»]
    3SERX-ray2.35A/C27-384[»]
    3SESX-ray1.90A/C27-384[»]
    3SETX-ray1.90A/C27-384[»]
    3SEUX-ray1.85A27-384[»]
    3SEVX-ray3.05A/C/E27-384[»]
    3SEWX-ray1.55A27-384[»]
    3SEXX-ray1.95A/C27-384[»]
    3SEYX-ray1.85A/C/E27-384[»]
    3VFJX-ray2.05A27-392[»]
    3W15X-ray1.80C27-396[»]
    3WAIX-ray1.90A27-392[»]
    3WOAX-ray2.00A27-393[»]
    4B3NX-ray3.30A/B27-395[»]
    4BL8X-ray3.04A/B27-393[»]
    4BL9X-ray2.80A/B/C/D27-393[»]
    4BLAX-ray3.50A/B/C/D27-393[»]
    4BLBX-ray2.80A/B/C/D27-393[»]
    4BLDX-ray2.80A/B/C/D27-393[»]
    4DXBX-ray2.29A/B27-396[»]
    4DXCX-ray2.30A27-396[»]
    4EDQX-ray1.64A/B27-384[»]
    4EGCX-ray1.99A27-392[»]
    4EXKX-ray1.28A26-392[»]
    4FE8X-ray3.00A/B/C27-384[»]
    4FEBX-ray2.80A/B/C27-384[»]
    4FECX-ray3.00A/B/C27-384[»]
    4FEDX-ray2.81A/B/C27-384[»]
    4GIZX-ray2.55A/B27-392[»]
    4GLIX-ray1.90A27-395[»]
    4IFPX-ray1.99A/B/C27-384[»]
    4IKMX-ray2.46A27-392[»]
    4IRLX-ray1.47A/B/C27-384[»]
    4JBZX-ray2.40A/B/C27-392[»]
    4JKMX-ray2.26C/D27-392[»]
    4KEGX-ray2.50A27-387[»]
    4KHZX-ray2.90E27-396[»]
    4KI0X-ray2.38E27-396[»]
    4KV3X-ray2.20A/B27-392[»]
    4KYCX-ray1.95A27-392[»]
    4KYDX-ray2.21A/B27-392[»]
    4KYEX-ray2.60A27-392[»]
    4LOGX-ray2.70A/B26-392[»]
    4MBPX-ray1.70A27-396[»]
    4N4XX-ray2.50A27-387[»]
    4NDZX-ray3.45A/B/C/D/E/F27-393[»]
    4NUFX-ray2.80A25-392[»]
    4O2XX-ray2.70A/B27-396[»]
    4OGMX-ray2.23A27-387[»]
    4PE2X-ray1.72A27-392[»]
    4PQKX-ray3.40A/B/C/D27-392[»]
    4QSZX-ray2.86A/B27-387[»]
    4QVHX-ray1.75A/B27-392[»]
    4R0YX-ray2.00A/B27-387[»]
    4RG5X-ray1.70A/B27-395[»]
    4RWFX-ray1.76A26-392[»]
    4RWGX-ray2.44A/B/C26-392[»]
    4TSMX-ray1.90A/B/C27-392[»]
    4WJVX-ray3.20E/F/G/H27-393[»]
    4WMSX-ray1.90A27-392[»]
    4WMTX-ray2.35A27-392[»]
    4WMUX-ray1.55A27-392[»]
    4WMVX-ray2.40A27-392[»]
    4WMWX-ray1.90A27-392[»]
    4WMXX-ray2.00A27-392[»]
    4WRNX-ray3.20A/B27-393[»]
    4WTHX-ray2.25A/B27-392[»]
    4WVHX-ray2.10A33-392[»]
    4XA2X-ray1.98A/B27-392[»]
    4XAJX-ray3.55A/B/C/D25-392[»]
    4XHSX-ray1.70A/B27-392[»]
    4XR8X-ray2.25A/B27-392[»]
    4XZSX-ray2.12A/B27-392[»]
    4XZVX-ray3.58A/C/E/G27-392[»]
    5AQ9X-ray1.86B/D33-392[»]
    5AZ6X-ray2.56A/B27-394[»]
    5AZ7X-ray1.96A27-394[»]
    5AZ8X-ray1.70A27-394[»]
    5AZ9X-ray1.82A27-394[»]
    5AZAX-ray2.08A27-394[»]
    5B3WX-ray2.40A/B27-393[»]
    5B3XX-ray2.40A27-393[»]
    5B3YX-ray1.90A27-392[»]
    5B3ZX-ray2.30A/B/C/D27-393[»]
    5BK1X-ray2.15A/B26-392[»]
    5BK2X-ray2.60A/B27-392[»]
    5C7RX-ray1.94A/B27-384[»]
    5CBNX-ray2.30B27-392[»]
    5CFVX-ray1.80A27-392[»]
    5DFMX-ray2.30A/B27-384[»]
    5DISX-ray2.85D32-387[»]
    5E24X-ray2.14A/C27-396[»]
    5EDUX-ray2.79A/B27-392[»]
    5FIOX-ray2.10B29-392[»]
    5FSGX-ray3.21A27-384[»]
    5GRUX-ray1.96A27-392[»]
    5GS2X-ray3.59A27-393[»]
    5GXTX-ray2.25A27-392[»]
    5GXVX-ray2.10A27-392[»]
    5H7NX-ray1.85A/B27-392[»]
    5H7QX-ray1.45A27-392[»]
    5HZ7X-ray1.43A27-392[»]
    5HZVX-ray2.70A27-393[»]
    5HZWX-ray4.45A27-393[»]
    5I04X-ray2.42A27-393[»]
    5I69X-ray2.70A27-392[»]
    5IHJX-ray2.20A27-392[»]
    5II5X-ray1.80A27-393[»]
    5IICX-ray2.90A/B27-393[»]
    5IQZX-ray2.33A27-392[»]
    5JJ4X-ray2.81A/B/C27-393[»]
    5JSTX-ray2.20A/B27-392[»]
    5JTQNMR-E/F108-149[»]
    5JTRNMR-E/F/G/H168-207[»]
    5LDFelectron microscopy6.20M/N/O/P/Q/R/S/T/U/V/W/X27-396[»]
    5M13X-ray1.37A27-392[»]
    5M14X-ray1.60A/B27-392[»]
    5M15X-ray1.90A/B27-392[»]
    5OSQX-ray2.05A/B27-393[»]
    5T03X-ray2.10A/B27-392[»]
    5T05X-ray1.95A/B27-392[»]
    5T0AX-ray1.95A/B27-392[»]
    5TTDX-ray2.00A/B33-392[»]
    5WPZX-ray2.00A/B/C/D/E/F27-392[»]
    5WQ6X-ray1.65A/B/C/D27-392[»]
    5ZCAX-ray1.80A28-392[»]
    5ZR0NMR-A238-266[»]
    6APXX-ray2.49A27-392[»]
    6D65X-ray2.35A/C27-391[»]
    6D66X-ray2.23A27-391[»]
    6D67X-ray2.55A27-391[»]
    6DM8X-ray2.70A/B/C/D/E/F/G/H27-392[»]
    ProteinModelPortaliP0AEX9
    SMRiP0AEX9
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4263065, 24 interactors
    852832, 1 interactor
    ComplexPortaliCPX-1932 Maltose transport complex
    DIPiDIP-31871N
    IntActiP0AEX9, 10 interactors
    MINTiP0AEX9
    STRINGi316385.ECDH10B_4223

    Chemistry databases

    DrugBankiDB02379 Beta-D-Glucose
    DB03995 Cyclo-Hepta-Amylose
    DB01914 D-glucose
    DB03323 Maltose

    Protein family/group databases

    TCDBi3.A.1.1.1 the atp-binding cassette (abc) superfamily

    2D gel databases

    SWISS-2DPAGEiP0AEX9

    Proteomic databases

    PaxDbiP0AEX9
    PRIDEiP0AEX9

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC77004; AAC77004; b4034
    BAE78036; BAE78036; BAE78036
    GeneIDi948538
    KEGGiecj:JW3994
    eco:b4034
    PATRICifig|1411691.4.peg.2677

    Organism-specific databases

    EchoBASEiEB0549
    EcoGeneiEG10554 malE

    Phylogenomic databases

    eggNOGiENOG4107FKK Bacteria
    COG2182 LUCA
    HOGENOMiHOG000055250
    InParanoidiP0AEX9
    KOiK10108
    PhylomeDBiP0AEX9

    Enzyme and pathway databases

    BioCyciEcoCyc:MALE-MONOMER
    MetaCyc:MALE-MONOMER

    Miscellaneous databases

    EvolutionaryTraceiP0AEX9
    PROiPR:P0AEX9

    Family and domain databases

    InterProiView protein in InterPro
    IPR006060 Maltose/Cyclodextrin-bd
    IPR006059 SBP
    IPR006061 SBP_1_CS
    PfamiView protein in Pfam
    PF13416 SBP_bac_8, 1 hit
    PRINTSiPR00181 MALTOSEBP
    PROSITEiView protein in PROSITE
    PS01037 SBP_BACTERIAL_1, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiMALE_ECOLI
    AccessioniPrimary (citable) accession number: P0AEX9
    Secondary accession number(s): P02928, Q2M6S0
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: November 7, 2018
    This is version 141 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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