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Protein

3',5'-cyclic adenosine monophosphate phosphodiesterase CpdA

Gene

cpdA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes cAMP to 5'-AMP. Plays an important regulatory role in modulating the intracellular concentration of cAMP, thereby influencing cAMP-dependent processes. Specific for cAMP.UniRule annotation1 Publication

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.UniRule annotation1 Publication

Cofactori

a metal cationUniRule annotationNote: Binds 2 metal cations per subunit.UniRule annotation

Enzyme regulationi

Activated by iron.1 Publication

Kineticsi

  1. KM=0.5 mM for cAMP1 Publication
  1. Vmax=2.0 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi22Metal cation 1UniRule annotation1
Metal bindingi24Metal cation 1UniRule annotation1
Binding sitei24cAMPUniRule annotation1
Metal bindingi64Metal cation 1UniRule annotation1
Metal bindingi64Metal cation 2UniRule annotation1
Binding sitei64cAMPUniRule annotation1
Metal bindingi94Metal cation 2UniRule annotation1
Metal bindingi164Metal cation 2UniRule annotation1
Metal bindingi203Metal cation 2UniRule annotation1
Metal bindingi205Metal cation 1UniRule annotation1
Binding sitei205cAMPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi94 – 95cAMPUniRule annotation2

GO - Molecular functioni

  • 3',5'-cyclic-AMP phosphodiesterase activity Source: EcoCyc
  • ferrous iron binding Source: EcoCyc
  • nucleotide binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandcAMP, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:G7579-MONOMER
MetaCyc:G7579-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
3',5'-cyclic adenosine monophosphate phosphodiesterase CpdAUniRule annotation (EC:3.1.4.53UniRule annotation)
Short name:
3',5'-cyclic AMP phosphodiesteraseUniRule annotation
Short name:
cAMP phosphodiesteraseUniRule annotation
Gene namesi
Name:cpdAUniRule annotation
Synonyms:icc
Ordered Locus Names:b3032, JW3000
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12187 cpdA

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000841461 – 2753',5'-cyclic adenosine monophosphate phosphodiesterase CpdAAdd BLAST275

Proteomic databases

EPDiP0AEW4
PaxDbiP0AEW4
PRIDEiP0AEW4

Interactioni

Protein-protein interaction databases

BioGridi4262395, 9 interactors
IntActiP0AEW4, 10 interactors
STRINGi316385.ECDH10B_3206

Structurei

3D structure databases

ProteinModelPortaliP0AEW4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cAMP phosphodiesterase class-III family.UniRule annotation

Phylogenomic databases

eggNOGiENOG41070EG Bacteria
COG1409 LUCA
HOGENOMiHOG000238351
InParanoidiP0AEW4
KOiK03651
OMAiCAWLDQH
PhylomeDBiP0AEW4

Family and domain databases

CDDicd07402 MPP_GpdQ, 1 hit
Gene3Di3.60.21.10, 1 hit
HAMAPiMF_00905 cAMP_phophodiest_CpdA, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR026575 cAMP_Pdiest_CpdA
IPR013622 CpdA_C
IPR029052 Metallo-depent_PP-like
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD587589 Calcineurin-like_phos_C, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEW4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESLLTLPLA GEARVRILQI TDTHLFAQKH EALLGVNTWE SYQAVLEAIR
60 70 80 90 100
PHQHEFDLIV ATGDLAQDQS SAAYQHFAEG IASFRAPCVW LPGNHDFQPA
110 120 130 140 150
MYSALQDAGI SPAKRVFIGE QWQILLLDSQ VFGVPHGELS EFQLEWLERK
160 170 180 190 200
LADAPERHTL LLLHHHPLPA GCSWLDQHSL RNAGELDTVL AKFPHVKYLL
210 220 230 240 250
CGHIHQELDL DWNGRRLLAT PSTCVQFKPH CSNFTLDTIA PGWRTLELHA
260 270
DGTLTTEVHR LADTRFQPDT ASEGY
Length:275
Mass (Da):30,938
Last modified:December 20, 2005 - v1
Checksum:i97696478536CFBF1
GO

Sequence cautioni

The sequence AAA69200 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16557 Genomic DNA Translation: BAA03989.1
U28377 Genomic DNA Translation: AAA69200.1 Different initiation.
U00096 Genomic DNA Translation: AAC76068.1
AP009048 Genomic DNA Translation: BAE77088.1
PIRiF65090
RefSeqiNP_417504.1, NC_000913.3
WP_000444747.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76068; AAC76068; b3032
BAE77088; BAE77088; BAE77088
GeneIDi947515
KEGGiecj:JW3000
eco:b3032
PATRICifig|1411691.4.peg.3699

Similar proteinsi

Entry informationi

Entry nameiCPDA_ECOLI
AccessioniPrimary (citable) accession number: P0AEW4
Secondary accession number(s): P36650, Q2M9G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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