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Protein

Chaperone protein Skp

Gene

skp

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Molecular chaperone that interacts specifically with outer membrane proteins, thus maintaining the solubility of early folding intermediates during passage through the periplasm. Required for the efficient release of OmpA from the inner membrane, the maintenance of its solubility in the periplasm, and, in association with lipopolysaccharide (LPS), for the efficient folding and insertion of OmpA into the outer membrane.5 Publications

Miscellaneous

Does not require ATP for its activity.

Caution

Was originally thought to bind DNA. It was probably an artifact due to the cationic nature of skp.Curated

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • lipopolysaccharide binding Source: EcoCyc
  • unfolded protein binding Source: EcoCyc

GO - Biological processi

  • chaperone-mediated protein folding Source: EcoCyc
  • Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
  • protein folding Source: EcoliWiki
  • protein insertion into membrane from inner side Source: EcoCyc
  • protein maturation by protein folding Source: CACAO
  • protein stabilization Source: EcoCyc

Keywordsi

Molecular functionChaperone

Enzyme and pathway databases

BioCyciEcoCyc:EG10455-MONOMER
MetaCyc:EG10455-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein Skp
Alternative name(s):
DNA-binding 17 kDa protein
Histone-like protein HLP-1
Seventeen kilodalton protein
Gene namesi
Name:skp
Synonyms:hlpA, ompH
Ordered Locus Names:b0178, JW0173
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10455 skp

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • outer membrane-bounded periplasmic space Source: EcoliWiki

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 202 PublicationsAdd BLAST20
ChainiPRO_000002017621 – 161Chaperone protein SkpAdd BLAST141

Proteomic databases

EPDiP0AEU7
PaxDbiP0AEU7
PRIDEiP0AEU7

Interactioni

Subunit structurei

Homotrimer. Interacts with OmpA. Interacts with PhoE during its translocation across the inner membrane, but, in contrast to OmpA, release of PhoE from the inner membrane is not dependent on skp. Interacts also with LamB, OmpC and OmpF.3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • unfolded protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263427, 241 interactors
DIPiDIP-36210N
IntActiP0AEU7, 30 interactors
STRINGi316385.ECDH10B_0158

Structurei

Secondary structure

1161
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 27Combined sources4
Helixi29 – 40Combined sources12
Turni41 – 46Combined sources6
Helixi48 – 65Combined sources18
Helixi78 – 90Combined sources13
Helixi92 – 104Combined sources13
Helixi106 – 130Combined sources25
Beta strandi134 – 138Combined sources5
Helixi139 – 141Combined sources3
Beta strandi142 – 145Combined sources4
Beta strandi149 – 151Combined sources3
Helixi153 – 159Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SG2X-ray2.35A/B/C20-161[»]
1U2MX-ray2.30A/B/C21-161[»]
ProteinModelPortaliP0AEU7
SMRiP0AEU7
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEU7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 108Lipopolysaccharide bindingSequence analysisAdd BLAST12

Domaini

Composed of a compact central beta-barrel domain with long alpha-helical extensions that form a three-pronged structure around an internal cavity. Substrate proteins may be bound in this cavity.

Sequence similaritiesi

Belongs to the Skp family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108RFI Bacteria
COG2825 LUCA
HOGENOMiHOG000261755
KOiK06142
OMAiYINPKHD
PhylomeDBiP0AEU7

Family and domain databases

Gene3Di3.30.910.20, 1 hit
InterProiView protein in InterPro
IPR005632 Chaperone_Skp
IPR024930 Skp_dom_sf
PANTHERiPTHR35089 PTHR35089, 1 hit
PfamiView protein in Pfam
PF03938 OmpH, 1 hit
PIRSFiPIRSF002094 OMP26_Skp, 1 hit
SMARTiView protein in SMART
SM00935 OmpH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEU7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKWLLAAGL GLALATSAQA ADKIAIVNMG SLFQQVAQKT GVSNTLENEF
60 70 80 90 100
KGRASELQRM ETDLQAKMKK LQSMKAGSDR TKLEKDVMAQ RQTFAQKAQA
110 120 130 140 150
FEQDRARRSN EERGKLVTRI QTAVKSVANS QDIDLVVDAN AVAYNSSDVK
160
DITADVLKQV K
Length:161
Mass (Da):17,688
Last modified:December 20, 2005 - v1
Checksum:i2A966BBD83F3E675
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15A → L in CAA53207 (PubMed:1991717).Curated1
Sequence conflicti149V → E in CAA53207 (PubMed:1991717).Curated1
Sequence conflicti153T → I in CAA53207 (PubMed:1991717).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21118 Genomic DNA Translation: AAA24630.1
X75465 Genomic DNA Translation: CAA53207.1
U70214 Genomic DNA Translation: AAB08607.1
U00096 Genomic DNA Translation: AAC73289.1
AP009048 Genomic DNA Translation: BAA77853.1
X54797 Genomic DNA Translation: CAA38567.1
PIRiJT0304 DNEC17
RefSeqiNP_414720.1, NC_000913.3
WP_000758956.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73289; AAC73289; b0178
BAA77853; BAA77853; BAA77853
GeneIDi944861
KEGGiecj:JW0173
eco:b0178
PATRICifig|1411691.4.peg.2101

Similar proteinsi

Entry informationi

Entry nameiSKP_ECOLI
AccessioniPrimary (citable) accession number: P0AEU7
Secondary accession number(s): P11457
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: March 28, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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