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UniProtKB - P0AET2 (HDEB_ECOLI)
Protein
Acid stress chaperone HdeB
Gene
hdeB
Organism
Escherichia coli (strain K12)
Status
Functioni
Required for optimal acid stress protection, which is important for survival of enteric bacteria in the acidic environment of the host stomach. Exhibits a chaperone-like activity at acidic pH by preventing the aggregation of many different periplasmic proteins.
UniRule annotation1 PublicationMiscellaneous
In vitro, HdeA is more efficient than HdeB at pH 2 and HdeB is more efficient than HdeA at pH 3. In vivo, both are required for optimal protection against acid stress at either pH 3 or pH 2 (PubMed:17085547).1 Publication
GO - Molecular functioni
- unfolded protein binding Source: EcoCyc
GO - Biological processi
- cellular stress response to acidic pH Source: UniProtKB-UniRule
- response to acidic pH Source: EcoCyc
Keywordsi
Molecular function | Chaperone |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11399-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Acid stress chaperone HdeBUniRule annotationAlternative name(s): 10K-L protein |
Gene namesi | Name:hdeBUniRule annotation Synonyms:yhhD, yhiC Ordered Locus Names:b3509, JW5669 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm UniRule annotation1 Publication
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Mutants display increased sensitivity to acid stress at pH 2 and pH 3.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 55 – 56 | WW → AA: Folded, but does not form homodimers at pH 7. 1 Publication | 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | UniRule annotation4 PublicationsAdd BLAST | 29 | |
ChainiPRO_0000021405 | 30 – 108 | Acid stress chaperone HdeBAdd BLAST | 79 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 93 | N6-acetyllysine1 Publication | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P0AET2 |
PaxDbi | P0AET2 |
PRIDEi | P0AET2 |
2D gel databases
SWISS-2DPAGEi | P0AET2 |
PTM databases
iPTMneti | P0AET2 |
Expressioni
Inductioni
Induced by the EvgS/EvgA two-component regulatory system. Negatively regulated by H-NS and the TorS/TorR two-component regulatory system.3 Publications
Interactioni
Subunit structurei
Homodimer at neutral pH. Dissociates into monomers at acidic pH.
2 PublicationsGO - Molecular functioni
- unfolded protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261137, 6 interactors |
DIPi | DIP-47949N |
IntActi | P0AET2, 3 interactors |
STRINGi | 511145.b3509 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AET2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AET2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the HdeB family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG50334GK, Bacteria |
HOGENOMi | CLU_149189_1_0_6 |
PhylomeDBi | P0AET2 |
Family and domain databases
Gene3Di | 1.10.890.10, 1 hit |
HAMAPi | MF_00947, HdeB, 1 hit |
InterProi | View protein in InterPro IPR038303, HdeA/HdeB_sf IPR028623, HdeB IPR010486, HNS-dep_expression_A/B |
Pfami | View protein in Pfam PF06411, HdeA, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AET2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNISSLRKAF IFMGAVAALS LVNAQSALAA NESAKDMTCQ EFIDLNPKAM
60 70 80 90 100
TPVAWWMLHE ETVYKGGDTV TLNETDLTQI PKVIEYCKKN PQKNLYTFKN
QASNDLPN
Sequence cautioni
The sequence AAB18485 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence BAA01884 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Mass spectrometryi
Molecular mass is 9065 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11109 Genomic DNA Translation: BAA01884.1 Different initiation. U00039 Genomic DNA Translation: AAB18485.1 Different initiation. U00096 Genomic DNA Translation: AAC76534.2 AP009048 Genomic DNA Translation: BAE77785.1 |
PIRi | S30269 |
RefSeqi | NP_417966.4, NC_000913.3 WP_001298717.1, NZ_STEB01000046.1 |
Genome annotation databases
EnsemblBacteriai | AAC76534; AAC76534; b3509 BAE77785; BAE77785; BAE77785 |
GeneIDi | 58463707 948026 |
KEGGi | ecj:JW5669 eco:b3509 |
PATRICi | fig|511145.12.peg.3616 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D11109 Genomic DNA Translation: BAA01884.1 Different initiation. U00039 Genomic DNA Translation: AAB18485.1 Different initiation. U00096 Genomic DNA Translation: AAC76534.2 AP009048 Genomic DNA Translation: BAE77785.1 |
PIRi | S30269 |
RefSeqi | NP_417966.4, NC_000913.3 WP_001298717.1, NZ_STEB01000046.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MYJ | NMR | - | A/B | 30-108 | [»] | |
2XUV | X-ray | 1.50 | A/B/C/D | 30-108 | [»] | |
SMRi | P0AET2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261137, 6 interactors |
DIPi | DIP-47949N |
IntActi | P0AET2, 3 interactors |
STRINGi | 511145.b3509 |
PTM databases
iPTMneti | P0AET2 |
2D gel databases
SWISS-2DPAGEi | P0AET2 |
Proteomic databases
jPOSTi | P0AET2 |
PaxDbi | P0AET2 |
PRIDEi | P0AET2 |
Genome annotation databases
EnsemblBacteriai | AAC76534; AAC76534; b3509 BAE77785; BAE77785; BAE77785 |
GeneIDi | 58463707 948026 |
KEGGi | ecj:JW5669 eco:b3509 |
PATRICi | fig|511145.12.peg.3616 |
Organism-specific databases
EchoBASEi | EB1371 |
Phylogenomic databases
eggNOGi | ENOG50334GK, Bacteria |
HOGENOMi | CLU_149189_1_0_6 |
PhylomeDBi | P0AET2 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11399-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AET2 |
PROi | PR:P0AET2 |
Family and domain databases
Gene3Di | 1.10.890.10, 1 hit |
HAMAPi | MF_00947, HdeB, 1 hit |
InterProi | View protein in InterPro IPR038303, HdeA/HdeB_sf IPR028623, HdeB IPR010486, HNS-dep_expression_A/B |
Pfami | View protein in Pfam PF06411, HdeA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HDEB_ECOLI | |
Accessioni | P0AET2Primary (citable) accession number: P0AET2 Secondary accession number(s): P26605, Q2M7H1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families