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Protein

Sodium/glutamate symporter

Gene

gltS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the sodium-dependent, binding-protein-independent transport of glutamate.UniRule annotation3 Publications

Enzyme regulationi

Inhibited by the uncoupler carbonylcyanide m-chlorophenylhydrazone (CCCP) and the ionophore monensin.1 Publication

Kineticsi

    1. Vmax=5.2 nmol/min/mg enzyme1 Publication

    GO - Molecular functioni

    • glutamate:sodium symporter activity Source: EcoCyc
    • symporter activity Source: EcoCyc

    GO - Biological processi

    Keywordsi

    Biological processAmino-acid transport, Ion transport, Sodium transport, Symport, Transport
    LigandSodium

    Enzyme and pathway databases

    BioCyciEcoCyc:GLTS-MONOMER
    MetaCyc:GLTS-MONOMER

    Protein family/group databases

    TCDBi2.A.27.1.1 the glutamate:na(+) symporter (ess) family

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/glutamate symporterUniRule annotationCurated
    Alternative name(s):
    Glutamate permease1 Publication
    Gene namesi
    Name:gltSUniRule annotation
    Synonyms:gltC
    Ordered Locus Names:b3653, JW3628
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10406 gltS

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 6Periplasmic2 Publications6
    Transmembranei7 – 24HelicalSequence analysisAdd BLAST18
    Topological domaini25 – 32Cytoplasmic2 Publications8
    Transmembranei33 – 52HelicalSequence analysisAdd BLAST20
    Topological domaini53 – 69Periplasmic2 PublicationsAdd BLAST17
    Transmembranei70 – 87HelicalSequence analysisAdd BLAST18
    Topological domaini88 – 93Cytoplasmic2 Publications6
    Transmembranei94 – 116HelicalSequence analysisAdd BLAST23
    Topological domaini117 – 156Periplasmic1 PublicationAdd BLAST40
    Transmembranei157 – 179HelicalSequence analysisAdd BLAST23
    Topological domaini180 – 212Cytoplasmic1 PublicationAdd BLAST33
    Transmembranei213 – 235HelicalSequence analysisAdd BLAST23
    Topological domaini236 – 244Periplasmic2 Publications9
    Transmembranei245 – 267HelicalSequence analysisAdd BLAST23
    Topological domaini268 – 276Cytoplasmic2 Publications9
    Transmembranei277 – 292HelicalSequence analysisAdd BLAST16
    Topological domaini293 – 301Periplasmic2 Publications9
    Transmembranei302 – 324HelicalSequence analysisAdd BLAST23
    Topological domaini325 – 367Cytoplasmic2 PublicationsAdd BLAST43
    Transmembranei368 – 390HelicalSequence analysisAdd BLAST23
    Topological domaini391 – 401Periplasmic2 Publications1 PublicationAdd BLAST11

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000523321 – 401Sodium/glutamate symporterAdd BLAST401

    Proteomic databases

    PaxDbiP0AER8
    PRIDEiP0AER8

    Interactioni

    Protein-protein interaction databases

    BioGridi4262574, 14 interactors
    DIPiDIP-48023N
    IntActiP0AER8, 1 interactor
    STRINGi316385.ECDH10B_3835

    Structurei

    3D structure databases

    ProteinModelPortaliP0AER8
    SMRiP0AER8
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamate:Na(+) symporter (ESS) (TC 2.A.27) family. [View classification]UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105D6U Bacteria
    COG0786 LUCA
    HOGENOMiHOG000282013
    InParanoidiP0AER8
    KOiK03312
    OMAiPTAMVNM
    PhylomeDBiP0AER8

    Family and domain databases

    HAMAPiMF_02062 GltS, 1 hit
    InterProiView protein in InterPro
    IPR004445 GltS
    PANTHERiPTHR36178 PTHR36178, 1 hit
    PfamiView protein in Pfam
    PF03616 Glt_symporter, 1 hit
    TIGRFAMsiTIGR00210 gltS, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0AER8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFHLDTLATL VAATLTLLLG RKLVHSVSFL KKYTIPEPVA GGLLVALALL
    60 70 80 90 100
    VLKKSMGWEV NFDMSLRDPL MLAFFATIGL NANIASLRAG GRVVGIFLIV
    110 120 130 140 150
    VVGLLVMQNA IGIGMASLLG LDPLMGLLAG SITLSGGHGT GAAWSKLFIE
    160 170 180 190 200
    RYGFTNATEV AMACATFGLV LGGLIGGPVA RYLVKHSTTP NGIPDDQEVP
    210 220 230 240 250
    TAFEKPDVGR MITSLVLIET IALIAICLTV GKIVAQLLAG TAFELPTFVC
    260 270 280 290 300
    VLFVGVILSN GLSIMGFYRV FERAVSVLGN VSLSLFLAMA LMGLKLWELA
    310 320 330 340 350
    SLALPMLAIL VVQTIFMALY AIFVTWRMMG KNYDAAVLAA GHCGFGLGAT
    360 370 380 390 400
    PTAIANMQAI TERFGPSHMA FLVVPMVGAF FIDIVNALVI KLYLMLPIFA

    G
    Length:401
    Mass (Da):42,425
    Last modified:December 20, 2005 - v1
    Checksum:i9569AE0AF75634EC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti378G → S in BAA00517 (PubMed:2254324).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D00626 Genomic DNA Translation: BAA00517.1
    X17499 Genomic DNA Translation: CAA35540.1
    L10328 Genomic DNA Translation: AAA62006.1
    U00096 Genomic DNA Translation: AAC76677.1
    AP009048 Genomic DNA Translation: BAE77640.1
    M64367 Genomic DNA Translation: AAA24514.1
    PIRiG65166 YOECNQ
    RefSeqiNP_418110.1, NC_000913.3
    WP_000468833.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76677; AAC76677; b3653
    BAE77640; BAE77640; BAE77640
    GeneIDi948166
    KEGGiecj:JW3628
    eco:b3653
    PATRICifig|1411691.4.peg.3053

    Similar proteinsi

    Entry informationi

    Entry nameiGLTS_ECOLI
    AccessioniPrimary (citable) accession number: P0AER8
    Secondary accession number(s): P19933, Q2M7W6
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: April 25, 2018
    This is version 101 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

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