UniProtKB - P0AER5 (GLTK_ECOLI)
Protein
Glutamate/aspartate import permease protein GltK
Gene
gltK
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex GltIJKL involved in glutamate and aspartate uptake. Probably responsible for the translocation of the substrate across the membrane.1 Publication
(Microbial infection) Probably transports the toxic C-terminal region of CdiA from P.luminescens strain TTO1 across the inner membrane to the cytoplasm, where CdiA has a toxic effect. Toxin transport is strain-specific, mutations in this gene do not confer resistance to several other tested CdiA toxins.1 Publication
GO - Molecular functioni
- transmembrane transporter activity Source: InterPro
GO - Biological processi
- amino acid transport Source: GO_Central
- toxin transport Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:GLTK-MONOMER MetaCyc:GLTK-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.3.4, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate/aspartate import permease protein GltKCurated |
Gene namesi | Name:gltK Ordered Locus Names:b0653, JW0648 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein Sequence analysis
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | PeriplasmicCuratedAdd BLAST | 19 | |
Transmembranei | 20 – 40 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 41 – 67 | CytoplasmicCuratedAdd BLAST | 27 | |
Transmembranei | 68 – 88 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 89 – 94 | PeriplasmicCurated | 6 | |
Transmembranei | 95 – 112 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 113 – 154 | CytoplasmicCuratedAdd BLAST | 42 | |
Transmembranei | 155 – 175 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 176 – 196 | PeriplasmicCuratedAdd BLAST | 21 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 224 | Cytoplasmic1 Publication | 7 |
GO - Cellular componenti
- ATP-binding cassette (ABC) transporter complex Source: InterPro
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Disruption confers resistance to cellular contact-dependent growth inhibition (CDI) CdiA of P.luminescens strain TTO1, but not to several other tested CdiA toxins.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060037 | 1 – 224 | Glutamate/aspartate import permease protein GltKAdd BLAST | 224 |
Proteomic databases
PaxDbi | P0AER5 |
PRIDEi | P0AER5 |
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (GltL), two transmembrane proteins (GltJ and GltK) and a solute-binding protein (GltI).
1 PublicationProtein-protein interaction databases
BioGRIDi | 4259913, 8 interactors |
ComplexPortali | CPX-4324, Glutamate/aspartate ABC transporter complex |
STRINGi | 511145.b0653 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 216 | ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST | 197 |
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0765, Bacteria |
HOGENOMi | CLU_019602_1_1_6 |
InParanoidi | P0AER5 |
PhylomeDBi | P0AER5 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR010065, AA_ABC_transptr_permease_3TM IPR043429, ArtM/GltK/GlnP/TcyL/YhdX-like IPR030205, GltK/AatM IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR30614, PTHR30614, 1 hit PTHR30614:SF1, PTHR30614:SF1, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR01726, HEQRo_perm_3TM, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AER5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MYEFDWSSIV PSLPYLLDGL VITLKITVTA VVIGILWGTM LAVMRLSSFA
60 70 80 90 100
PVAWFAKAYV NVFRSIPLVM VLLWFYLIVP GFLQNVLGLS PKNDIRLISA
110 120 130 140 150
MVAFSMFEAA YYSEIIRAGI QSISRGQSSA ALALGMTHWQ SMKLIILPQA
160 170 180 190 200
FRAMVPLLLT QGIVLFQDTS LVYVLSLADF FRTASTIGER DGTQVEMILF
210 220
AGFVYFVISL SASLLVSYLK RRTA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10981 Genomic DNA Translation: AAA60981.1 U82598 Genomic DNA Translation: AAB40854.1 U00096 Genomic DNA Translation: AAC73754.1 AP009048 Genomic DNA Translation: BAA35305.1 |
PIRi | C64800 |
RefSeqi | NP_415186.1, NC_000913.3 WP_000272824.1, NZ_STEB01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC73754; AAC73754; b0653 BAA35305; BAA35305; BAA35305 |
GeneIDi | 48727512 947354 |
KEGGi | ecj:JW0648 eco:b0653 |
PATRICi | fig|1411691.4.peg.1615 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U10981 Genomic DNA Translation: AAA60981.1 U82598 Genomic DNA Translation: AAB40854.1 U00096 Genomic DNA Translation: AAC73754.1 AP009048 Genomic DNA Translation: BAA35305.1 |
PIRi | C64800 |
RefSeqi | NP_415186.1, NC_000913.3 WP_000272824.1, NZ_STEB01000031.1 |
3D structure databases
SMRi | P0AER5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259913, 8 interactors |
ComplexPortali | CPX-4324, Glutamate/aspartate ABC transporter complex |
STRINGi | 511145.b0653 |
Protein family/group databases
TCDBi | 3.A.1.3.4, the atp-binding cassette (abc) superfamily |
Proteomic databases
PaxDbi | P0AER5 |
PRIDEi | P0AER5 |
Genome annotation databases
EnsemblBacteriai | AAC73754; AAC73754; b0653 BAA35305; BAA35305; BAA35305 |
GeneIDi | 48727512 947354 |
KEGGi | ecj:JW0648 eco:b0653 |
PATRICi | fig|1411691.4.peg.1615 |
Organism-specific databases
EchoBASEi | EB2528 |
Phylogenomic databases
eggNOGi | COG0765, Bacteria |
HOGENOMi | CLU_019602_1_1_6 |
InParanoidi | P0AER5 |
PhylomeDBi | P0AER5 |
Enzyme and pathway databases
BioCyci | EcoCyc:GLTK-MONOMER MetaCyc:GLTK-MONOMER |
Miscellaneous databases
PROi | PR:P0AER5 |
Family and domain databases
CDDi | cd06261, TM_PBP2, 1 hit |
Gene3Di | 1.10.3720.10, 1 hit |
InterProi | View protein in InterPro IPR010065, AA_ABC_transptr_permease_3TM IPR043429, ArtM/GltK/GlnP/TcyL/YhdX-like IPR030205, GltK/AatM IPR000515, MetI-like IPR035906, MetI-like_sf |
PANTHERi | PTHR30614, PTHR30614, 1 hit PTHR30614:SF1, PTHR30614:SF1, 1 hit |
Pfami | View protein in Pfam PF00528, BPD_transp_1, 1 hit |
SUPFAMi | SSF161098, SSF161098, 1 hit |
TIGRFAMsi | TIGR01726, HEQRo_perm_3TM, 1 hit |
PROSITEi | View protein in PROSITE PS50928, ABC_TM1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GLTK_ECOLI | |
Accessioni | P0AER5Primary (citable) accession number: P0AER5 Secondary accession number(s): P41075 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families