UniProtKB - P0AEP7 (GCL_ECOLI)
Protein
Glyoxylate carboligase
Gene
gcl
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde).
Catalytic activityi
- EC:4.1.1.47
Cofactori
Protein has several cofactor binding sites:- Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity
- thiamine diphosphateBy similarityNote: Binds 1 thiamine pyrophosphate per subunit.By similarity
: glycolate degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.Proteins known to be involved in the 4 steps of the subpathway in this organism are:
- no protein annotated in this organism
- Glyoxylate carboligase (gcl)
- 2-hydroxy-3-oxopropionate reductase (glxR)
- Glycerate 3-kinase (glxK)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.
GO - Molecular functioni
- FAD binding Source: EcoCyc
- flavin adenine dinucleotide binding Source: GO_Central
- identical protein binding Source: EcoCyc
- magnesium ion binding Source: InterPro
- tartronate-semialdehyde synthase activity Source: EcoCyc
- thiamine pyrophosphate binding Source: EcoCyc
GO - Biological processi
- glycolate catabolic process Source: EcoCyc
- glyoxylate catabolic process Source: EcoCyc
- isoleucine biosynthetic process Source: GO_Central
- valine biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | EcoCyc:GLYOCARBOLIG-MONOMER MetaCyc:GLYOCARBOLIG-MONOMER |
BRENDAi | 4.1.1.47, 2165 |
UniPathwayi | UPA00864;UER00831 |
Names & Taxonomyi
Protein namesi | Recommended name: Glyoxylate carboligase (EC:4.1.1.47)Alternative name(s): Tartronate-semialdehyde synthase |
Gene namesi | Name:gcl Ordered Locus Names:b0507, JW0495 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000090832 | 2 – 593 | Glyoxylate carboligaseAdd BLAST | 592 |
Proteomic databases
PaxDbi | P0AEP7 |
PRIDEi | P0AEP7 |
Expressioni
Inductioni
By glyoxylate.
Interactioni
Subunit structurei
Homotetramer.
GO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4263336, 2 interactors |
DIPi | DIP-48112N |
IntActi | P0AEP7, 6 interactors |
STRINGi | 511145.b0507 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEP7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEP7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the TPP enzyme family.Curated
Phylogenomic databases
eggNOGi | COG3960, Bacteria |
HOGENOMi | CLU_013748_1_2_6 |
InParanoidi | P0AEP7 |
PhylomeDBi | P0AEP7 |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR006397, Glyox_carbo_lig IPR029061, THDP-binding IPR012000, Thiamin_PyroP_enz_cen_dom IPR012001, Thiamin_PyroP_enz_TPP-bd_dom IPR011766, TPP_enzyme-bd_C |
PANTHERi | PTHR18968:SF14, PTHR18968:SF14, 1 hit |
Pfami | View protein in Pfam PF02775, TPP_enzyme_C, 1 hit PF00205, TPP_enzyme_M, 1 hit PF02776, TPP_enzyme_N, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit SSF52518, SSF52518, 2 hits |
TIGRFAMsi | TIGR01504, glyox_carbo_lig, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEP7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKMRAVDAA MYVLEKEGIT TAFGVPGAAI NPFYSAMRKH GGIRHILARH
60 70 80 90 100
VEGASHMAEG YTRATAGNIG VCLGTSGPAG TDMITALYSA SADSIPILCI
110 120 130 140 150
TGQAPRARLH KEDFQAVDIE AIAKPVSKMA VTVREAALVP RVLQQAFHLM
160 170 180 190 200
RSGRPGPVLV DLPFDVQVAE IEFDPDMYEP LPVYKPAASR MQIEKAVEML
210 220 230 240 250
IQAERPVIVA GGGVINADAA ALLQQFAELT SVPVIPTLMG WGCIPDDHEL
260 270 280 290 300
MAGMVGLQTA HRYGNATLLA SDMVFGIGNR FANRHTGSVE KYTEGRKIVH
310 320 330 340 350
IDIEPTQIGR VLCPDLGIVS DAKAALTLLV EVAQEMQKAG RLPCRKEWVA
360 370 380 390 400
DCQQRKRTLL RKTHFDNVPV KPQRVYEEMN KAFGRDVCYV TTIGLSQIAA
410 420 430 440 450
AQMLHVFKDR HWINCGQAGP LGWTIPAALG VCAADPKRNV VAISGDFDFQ
460 470 480 490 500
FLIEELAVGA QFNIPYIHVL VNNAYLGLIR QSQRAFDMDY CVQLAFENIN
510 520 530 540 550
SSEVNGYGVD HVKVAEGLGC KAIRVFKPED IAPAFEQAKA LMAQYRVPVV
560 570 580 590
VEVILERVTN ISMGSELDNV MEFEDIADNA ADAPTETCFM HYE
Sequence cautioni
The sequence AAB40260 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03845 Genomic DNA Translation: AAA23864.1 U82664 Genomic DNA Translation: AAB40260.1 Different initiation. U00096 Genomic DNA Translation: AAC73609.1 AP009048 Genomic DNA Translation: BAE76285.1 |
PIRi | JT0742 |
RefSeqi | NP_415040.1, NC_000913.3 WP_001096881.1, NZ_STEB01000007.1 |
Genome annotation databases
EnsemblBacteriai | AAC73609; AAC73609; b0507 BAE76285; BAE76285; BAE76285 |
GeneIDi | 945394 |
KEGGi | ecj:JW0495 eco:b0507 |
PATRICi | fig|1411691.4.peg.1770 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L03845 Genomic DNA Translation: AAA23864.1 U82664 Genomic DNA Translation: AAB40260.1 Different initiation. U00096 Genomic DNA Translation: AAC73609.1 AP009048 Genomic DNA Translation: BAE76285.1 |
PIRi | JT0742 |
RefSeqi | NP_415040.1, NC_000913.3 WP_001096881.1, NZ_STEB01000007.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2PAN | X-ray | 2.70 | A/B/C/D/E/F | 1-593 | [»] | |
SMRi | P0AEP7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4263336, 2 interactors |
DIPi | DIP-48112N |
IntActi | P0AEP7, 6 interactors |
STRINGi | 511145.b0507 |
Proteomic databases
PaxDbi | P0AEP7 |
PRIDEi | P0AEP7 |
Genome annotation databases
EnsemblBacteriai | AAC73609; AAC73609; b0507 BAE76285; BAE76285; BAE76285 |
GeneIDi | 945394 |
KEGGi | ecj:JW0495 eco:b0507 |
PATRICi | fig|1411691.4.peg.1770 |
Organism-specific databases
EchoBASEi | EB1542 |
Phylogenomic databases
eggNOGi | COG3960, Bacteria |
HOGENOMi | CLU_013748_1_2_6 |
InParanoidi | P0AEP7 |
PhylomeDBi | P0AEP7 |
Enzyme and pathway databases
UniPathwayi | UPA00864;UER00831 |
BioCyci | EcoCyc:GLYOCARBOLIG-MONOMER MetaCyc:GLYOCARBOLIG-MONOMER |
BRENDAi | 4.1.1.47, 2165 |
Miscellaneous databases
EvolutionaryTracei | P0AEP7 |
PROi | PR:P0AEP7 |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR006397, Glyox_carbo_lig IPR029061, THDP-binding IPR012000, Thiamin_PyroP_enz_cen_dom IPR012001, Thiamin_PyroP_enz_TPP-bd_dom IPR011766, TPP_enzyme-bd_C |
PANTHERi | PTHR18968:SF14, PTHR18968:SF14, 1 hit |
Pfami | View protein in Pfam PF02775, TPP_enzyme_C, 1 hit PF00205, TPP_enzyme_M, 1 hit PF02776, TPP_enzyme_N, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit SSF52518, SSF52518, 2 hits |
TIGRFAMsi | TIGR01504, glyox_carbo_lig, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GCL_ECOLI | |
Accessioni | P0AEP7Primary (citable) accession number: P0AEP7 Secondary accession number(s): P30146, P77126, Q2MBS1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 106 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families