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Protein

NAD(P)H-flavin reductase

Gene

fre

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.

Caution

Was originally assigned to be ubiB.1 Publication

Catalytic activityi

Reduced riboflavin + NAD(P)+ = riboflavin + NAD(P)H.
2 cob(II)alamin + NAD+ + 2 H2O = 2 aquacob(III)alamin + NADH.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 115PyridineBy similarity5

GO - Molecular functioni

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • iron ion homeostasis Source: UniProtKB-KW
  • protein repair Source: EcoCyc
  • response to oxidative stress Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processIon transport, Iron transport, Transport
LigandFAD, Flavoprotein, FMN, Iron, NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:FMNREDUCT-MONOMER
MetaCyc:FMNREDUCT-MONOMER
BRENDAi1.5.1.41 2026

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-flavin reductase (EC:1.16.1.3, EC:1.5.1.41)
Alternative name(s):
Aquacobalamin reductase
FMN reductase
Ferrisiderophore reductase C
NAD(P)H:flavin oxidoreductase
Riboflavin reductase [NAD(P)H]
Gene namesi
Name:fre
Synonyms:fadI, flrD, fsrC, ubiB
Ordered Locus Names:b3844, JW3820
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10334 fre

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000681442 – 233NAD(P)H-flavin reductaseAdd BLAST232

Proteomic databases

EPDiP0AEN1
PaxDbiP0AEN1
PRIDEiP0AEN1

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262003, 14 interactors
IntActiP0AEN1, 13 interactors
STRINGi316385.ECDH10B_4033

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AEN1
SMRiP0AEN1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEN1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 99FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108EX3 Bacteria
COG0543 LUCA
HOGENOMiHOG000269618
InParanoidiP0AEN1
KOiK05368
PhylomeDBiP0AEN1

Family and domain databases

Gene3Di3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR017927 FAD-bd_FR_type
IPR039261 FNR_nucleotide-bd
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00410 PHEHYDRXLASE
SUPFAMiSSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEN1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTLSCKVTS VEAITDTVYR VRIVPDAAFS FRAGQYLMVV MDERDKRPFS
60 70 80 90 100
MASTPDEKGF IELHIGASEI NLYAKAVMDR ILKDHQIVVD IPHGEAWLRD
110 120 130 140 150
DEERPMILIA GGTGFSYARS ILLTALARNP NRDITIYWGG REEQHLYDLC
160 170 180 190 200
ELEALSLKHP GLQVVPVVEQ PEAGWRGRTG TVLTAVLQDH GTLAEHDIYI
210 220 230
AGRFEMAKIA RDLFCSERNA REDRLFGDAF AFI
Length:233
Mass (Da):26,242
Last modified:January 23, 2007 - v2
Checksum:i0E64DE5A8FA0F12D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61182 Genomic DNA Translation: AAA23806.1
M85227 Genomic DNA Translation: AAA23753.1
M74448 Genomic DNA Translation: AAA91058.1
M87049 Genomic DNA Translation: AAA67641.1
U00096 Genomic DNA Translation: AAC76847.1
AP009048 Genomic DNA Translation: BAE77459.1
PIRiA39434
RefSeqiNP_418286.1, NC_000913.3
WP_000209826.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76847; AAC76847; b3844
BAE77459; BAE77459; BAE77459
GeneIDi948325
KEGGiecj:JW3820
eco:b3844
PATRICifig|511145.12.peg.3958

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61182 Genomic DNA Translation: AAA23806.1
M85227 Genomic DNA Translation: AAA23753.1
M74448 Genomic DNA Translation: AAA91058.1
M87049 Genomic DNA Translation: AAA67641.1
U00096 Genomic DNA Translation: AAC76847.1
AP009048 Genomic DNA Translation: BAE77459.1
PIRiA39434
RefSeqiNP_418286.1, NC_000913.3
WP_000209826.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QFJX-ray2.20A/B/C/D2-233[»]
ProteinModelPortaliP0AEN1
SMRiP0AEN1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262003, 14 interactors
IntActiP0AEN1, 13 interactors
STRINGi316385.ECDH10B_4033

Proteomic databases

EPDiP0AEN1
PaxDbiP0AEN1
PRIDEiP0AEN1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76847; AAC76847; b3844
BAE77459; BAE77459; BAE77459
GeneIDi948325
KEGGiecj:JW3820
eco:b3844
PATRICifig|511145.12.peg.3958

Organism-specific databases

EchoBASEiEB0330
EcoGeneiEG10334 fre

Phylogenomic databases

eggNOGiENOG4108EX3 Bacteria
COG0543 LUCA
HOGENOMiHOG000269618
InParanoidiP0AEN1
KOiK05368
PhylomeDBiP0AEN1

Enzyme and pathway databases

BioCyciEcoCyc:FMNREDUCT-MONOMER
MetaCyc:FMNREDUCT-MONOMER
BRENDAi1.5.1.41 2026

Miscellaneous databases

EvolutionaryTraceiP0AEN1
PROiPR:P0AEN1

Family and domain databases

Gene3Di3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR008333 Cbr1-like_FAD-bd_dom
IPR017927 FAD-bd_FR_type
IPR039261 FNR_nucleotide-bd
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00410 PHEHYDRXLASE
SUPFAMiSSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFRE_ECOLI
AccessioniPrimary (citable) accession number: P0AEN1
Secondary accession number(s): P23486, P76768, Q2M8E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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