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Protein

NAD(P)H-flavin reductase

Gene

fre

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of soluble flavins by reduced pyridine nucleotides. Seems to reduce the complexed Fe3+ iron of siderophores to Fe2+, thus releasing it from the chelator.

Caution

Was originally assigned to be ubiB.1 Publication

Catalytic activityi

Reduced riboflavin + NAD(P)+ = riboflavin + NAD(P)H.
2 cob(II)alamin + NAD+ + 2 H2O = 2 aquacob(III)alamin + NADH.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi111 – 115PyridineBy similarity5

GO - Molecular functioni

  • aquacobalamin reductase activity Source: UniProtKB-EC
  • enzyme activator activity Source: EcoCyc
  • riboflavin reductase (NADH) activity Source: EcoCyc
  • riboflavin reductase (NADPH) activity Source: EcoCyc

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • iron ion homeostasis Source: UniProtKB-KW
  • protein repair Source: EcoCyc
  • response to oxidative stress Source: EcoCyc

Keywordsi

Molecular functionOxidoreductase
Biological processIon transport, Iron transport, Transport
LigandFAD, Flavoprotein, FMN, Iron, NAD, NADP

Enzyme and pathway databases

BioCyciEcoCyc:FMNREDUCT-MONOMER
MetaCyc:FMNREDUCT-MONOMER
BRENDAi1.5.1.41 2026

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H-flavin reductase (EC:1.16.1.3, EC:1.5.1.41)
Alternative name(s):
Aquacobalamin reductase
FMN reductase
Ferrisiderophore reductase C
NAD(P)H:flavin oxidoreductase
Riboflavin reductase [NAD(P)H]
Gene namesi
Name:fre
Synonyms:fadI, flrD, fsrC, ubiB
Ordered Locus Names:b3844, JW3820
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10334 fre

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000681442 – 233NAD(P)H-flavin reductaseAdd BLAST232

Proteomic databases

EPDiP0AEN1
PaxDbiP0AEN1
PRIDEiP0AEN1

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262003, 14 interactors
IntActiP0AEN1, 13 interactors
STRINGi316385.ECDH10B_4033

Structurei

Secondary structure

1233
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 17Combined sources15
Beta strandi19 – 27Combined sources9
Beta strandi36 – 44Combined sources9
Beta strandi46 – 50Combined sources5
Beta strandi61 – 65Combined sources5
Helixi75 – 84Combined sources10
Beta strandi85 – 93Combined sources9
Beta strandi102 – 104Combined sources3
Beta strandi106 – 111Combined sources6
Helixi115 – 128Combined sources14
Beta strandi134 – 142Combined sources9
Helixi143 – 145Combined sources3
Helixi149 – 158Combined sources10
Beta strandi162 – 170Combined sources9
Beta strandi177 – 180Combined sources4
Helixi182 – 189Combined sources8
Beta strandi197 – 202Combined sources6
Helixi204 – 217Combined sources14
Helixi222 – 224Combined sources3
Helixi229 – 232Combined sources4

3D structure databases

ProteinModelPortaliP0AEN1
SMRiP0AEN1
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEN1

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 99FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108EX3 Bacteria
COG0543 LUCA
HOGENOMiHOG000269618
InParanoidiP0AEN1
KOiK05368
OMAiKAGQYLM
PhylomeDBiP0AEN1

Family and domain databases

Gene3Di3.40.50.80, 1 hit
InterProiView protein in InterPro
IPR017927 Fd_Rdtase_FAD-bd
IPR039261 FNR_nucleotide_bd
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR001221 Phe_hydroxylase
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00970 FAD_binding_6, 1 hit
PF00175 NAD_binding_1, 1 hit
PRINTSiPR00410 PHEHYDRXLASE
SUPFAMiSSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEN1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLSCKVTS VEAITDTVYR VRIVPDAAFS FRAGQYLMVV MDERDKRPFS
60 70 80 90 100
MASTPDEKGF IELHIGASEI NLYAKAVMDR ILKDHQIVVD IPHGEAWLRD
110 120 130 140 150
DEERPMILIA GGTGFSYARS ILLTALARNP NRDITIYWGG REEQHLYDLC
160 170 180 190 200
ELEALSLKHP GLQVVPVVEQ PEAGWRGRTG TVLTAVLQDH GTLAEHDIYI
210 220 230
AGRFEMAKIA RDLFCSERNA REDRLFGDAF AFI
Length:233
Mass (Da):26,242
Last modified:January 23, 2007 - v2
Checksum:i0E64DE5A8FA0F12D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61182 Genomic DNA Translation: AAA23806.1
M85227 Genomic DNA Translation: AAA23753.1
M74448 Genomic DNA Translation: AAA91058.1
M87049 Genomic DNA Translation: AAA67641.1
U00096 Genomic DNA Translation: AAC76847.1
AP009048 Genomic DNA Translation: BAE77459.1
PIRiA39434
RefSeqiNP_418286.1, NC_000913.3
WP_000209826.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76847; AAC76847; b3844
BAE77459; BAE77459; BAE77459
GeneIDi948325
KEGGiecj:JW3820
eco:b3844
PATRICifig|511145.12.peg.3958

Similar proteinsi

Entry informationi

Entry nameiFRE_ECOLI
AccessioniPrimary (citable) accession number: P0AEN1
Secondary accession number(s): P23486, P76768, Q2M8E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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