UniProtKB - P0AEM9 (TCYJ_ECOLI)
Protein
L-cystine-binding protein TcyJ
Gene
tcyJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the ABC transporter complex TcyJLN involved in L-cystine import (PubMed:25139244, PubMed:20351115, PubMed:25837721, PubMed:26350134). This high affinity cystine transporter is involved in resistance to oxidative stress by forming a L-cysteine/L-cystine shuttle system with the EamA transporter, which exports L-cysteine as reducing equivalents to the periplasm to prevent the cells from oxidative stress. Exported L-cysteine can reduce the periplasmic hydrogen peroxide to water, and then generated L-cystine is imported back into the cytoplasm via the TcyJLN complex (PubMed:20351115, PubMed:25837721). Functions at low cystine concentrations (PubMed:26350134). The system can also transport L-cysteine, diaminopimelic acid (DAP), djenkolate, lanthionine, D-cystine, homocystine, and it mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys) (PubMed:25139244, PubMed:26350134). Binds cystine and DAP (PubMed:4564569, PubMed:8450713).6 Publications
Activity regulationi
The TcyJLN system is inhibited by L-cystine, L-cysteine, DL-2,6-diaminopimelic acid and L-cystathionine, and is stimulated by D-cysteine.1 Publication
GO - Molecular functioni
- amino acid binding Source: EcoCyc
- L-cystine transmembrane transporter activity Source: EcoCyc
- ligand-gated ion channel activity Source: InterPro
GO - Biological processi
- L-cystine transport Source: EcoCyc
- sulfur utilization Source: EcoCyc
Keywordsi
Biological process | Amino-acid transport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:G7039-MONOMER MetaCyc:G7039-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: L-cystine-binding protein TcyJCuratedShort name: CBP Alternative name(s): Protein FliY Sulfate starvation-induced protein 7 Short name: SSI7 |
Gene namesi | Name:tcyJ1 Publication Synonyms:fliY1 Publication, yzzR Ordered Locus Names:b1920, JW1905 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Periplasm 1 Publication
GO - Cellular componenti
- membrane Source: InterPro
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Disruption phenotypei
Disruption of the gene significantly impairs intracellular uptake of L-cystine (PubMed:25837721, PubMed:20351115). Mutants show a higher sensitivity to H2O2 than wild-type cells (PubMed:20351115). The tcyJ-tcyP double mutant is unable to import cystine and is completely resistant to both L-selenaproline and L-selenocystine (PubMed:25139244, PubMed:26350134). The double disruption of eamA and tcyJ increases cellular levels of lipid peroxides (PubMed:25837721).4 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | 3 PublicationsAdd BLAST | 29 | |
ChainiPRO_0000031755 | 30 – 266 | L-cystine-binding protein TcyJAdd BLAST | 237 |
Proteomic databases
jPOSTi | P0AEM9 |
PaxDbi | P0AEM9 |
PRIDEi | P0AEM9 |
2D gel databases
SWISS-2DPAGEi | P0AEM9 |
Expressioni
Inductioni
Interactioni
Subunit structurei
The complex is composed of two ATP-binding proteins (TcyN), two transmembrane proteins (TcyL) and a solute-binding protein (TcyJ).
2 PublicationsProtein-protein interaction databases
BioGRIDi | 4261251, 71 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
DIPi | DIP-47980N |
IntActi | P0AEM9, 9 interactors |
STRINGi | 511145.b1920 |
Family & Domainsi
Sequence similaritiesi
Belongs to the bacterial solute-binding protein 3 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0834, Bacteria |
HOGENOMi | CLU_019602_18_5_6 |
InParanoidi | P0AEM9 |
PhylomeDBi | P0AEM9 |
Family and domain databases
InterProi | View protein in InterPro IPR001320, Iontro_rcpt IPR018313, SBP_3_CS IPR001638, Solute-binding_3/MltF_N |
Pfami | View protein in Pfam PF00497, SBP_bac_3, 1 hit |
SMARTi | View protein in SMART SM00062, PBPb, 1 hit SM00079, PBPe, 1 hit |
PROSITEi | View protein in PROSITE PS01039, SBP_BACTERIAL_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEM9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLAHLGRQA LMGVMAVALV AGMSVKSFAD EGLLNKVKER GTLLVGLEGT
60 70 80 90 100
YPPFSFQGDD GKLTGFEVEF AQQLAKHLGV EASLKPTKWD GMLASLDSKR
110 120 130 140 150
IDVVINQVTI SDERKKKYDF STPYTISGIQ ALVKKGNEGT IKTADDLKGK
160 170 180 190 200
KVGVGLGTNY EEWLRQNVQG VDVRTYDDDP TKYQDLRVGR IDAILVDRLA
210 220 230 240 250
ALDLVKKTND TLAVTGEAFS RQESGVALRK GNEDLLKAVN DAIAEMQKDG
260
TLQALSEKWF GADVTK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18539 Genomic DNA Translation: AAC43545.1 U00096 Genomic DNA Translation: AAC74987.1 AP009048 Genomic DNA Translation: BAA15740.1 |
PIRi | E64955 |
RefSeqi | NP_416430.1, NC_000913.3 WP_001296168.1, NZ_SSUV01000013.1 |
Genome annotation databases
EnsemblBacteriai | AAC74987; AAC74987; b1920 BAA15740; BAA15740; BAA15740 |
GeneIDi | 49584330 948833 |
KEGGi | ecj:JW1905 eco:b1920 |
PATRICi | fig|511145.12.peg.2002 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U18539 Genomic DNA Translation: AAC43545.1 U00096 Genomic DNA Translation: AAC74987.1 AP009048 Genomic DNA Translation: BAA15740.1 |
PIRi | E64955 |
RefSeqi | NP_416430.1, NC_000913.3 WP_001296168.1, NZ_SSUV01000013.1 |
3D structure databases
SMRi | P0AEM9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261251, 71 interactors |
ComplexPortali | CPX-4406, L-cystine ABC transporter complex |
DIPi | DIP-47980N |
IntActi | P0AEM9, 9 interactors |
STRINGi | 511145.b1920 |
Protein family/group databases
TCDBi | 3.A.1.3.10, the atp-binding cassette (abc) superfamily |
2D gel databases
SWISS-2DPAGEi | P0AEM9 |
Proteomic databases
jPOSTi | P0AEM9 |
PaxDbi | P0AEM9 |
PRIDEi | P0AEM9 |
Genome annotation databases
EnsemblBacteriai | AAC74987; AAC74987; b1920 BAA15740; BAA15740; BAA15740 |
GeneIDi | 49584330 948833 |
KEGGi | ecj:JW1905 eco:b1920 |
PATRICi | fig|511145.12.peg.2002 |
Organism-specific databases
EchoBASEi | EB2545 |
Phylogenomic databases
eggNOGi | COG0834, Bacteria |
HOGENOMi | CLU_019602_18_5_6 |
InParanoidi | P0AEM9 |
PhylomeDBi | P0AEM9 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7039-MONOMER MetaCyc:G7039-MONOMER |
Miscellaneous databases
PROi | PR:P0AEM9 |
Family and domain databases
InterProi | View protein in InterPro IPR001320, Iontro_rcpt IPR018313, SBP_3_CS IPR001638, Solute-binding_3/MltF_N |
Pfami | View protein in Pfam PF00497, SBP_bac_3, 1 hit |
SMARTi | View protein in SMART SM00062, PBPb, 1 hit SM00079, PBPe, 1 hit |
PROSITEi | View protein in PROSITE PS01039, SBP_BACTERIAL_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCYJ_ECOLI | |
Accessioni | P0AEM9Primary (citable) accession number: P0AEM9 Secondary accession number(s): P39174 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families