UniProtKB - P0AEK7 (FDNI_ECOLI)
Protein
Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunit
Gene
fdnI
Organism
Escherichia coli (strain K12)
Status
Functioni
Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. Subunit gamma is the cytochrome b556 component of the formate dehydrogenase-N, and also contains a menaquinone reduction site that receives electrons from the beta subunit (FdnH), through its hemes. Formate dehydrogenase-N is part of a system that generates proton motive force, together with the dissimilatory nitrate reductase (Nar).1 Publication
Cofactori
heme1 PublicationNote: Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 18 | Iron (heme B 1 axial ligand); via tele nitrogen | 1 | |
Metal bindingi | 57 | Iron (heme B 2 axial ligand); via tele nitrogen | 1 | |
Metal bindingi | 155 | Iron (heme B 2 axial ligand); via tele nitrogen | 1 | |
Metal bindingi | 169 | Iron (heme B 1 axial ligand); via tele nitrogen | 1 | |
Binding sitei | 169 | Menaquinone; via pros nitrogen | 1 |
GO - Molecular functioni
- electron transfer activity Source: EcoCyc
- formate dehydrogenase (NAD+) activity Source: InterPro
- formate dehydrogenase (quinone) activity Source: EcoCyc
- heme binding Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- anaerobic respiration Source: EcoCyc
- formate oxidation Source: EcoCyc
- respiratory electron transport chain Source: InterPro
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:FDNI-MONOMER MetaCyc:FDNI-MONOMER |
BRENDAi | 1.1.5.6, 2026 |
Protein family/group databases
TCDBi | 5.A.3.2.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | Recommended name: Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunitAlternative name(s): Anaerobic formate dehydrogenase cytochrome b556 subunit Formate dehydrogenase-N subunit gamma Short name: FDH-N subunit gamma |
Gene namesi | Name:fdnI Ordered Locus Names:b1476, JW1472 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Other locations
- formate dehydrogenase complex Source: EcoCyc
- integral component of membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | Cytoplasmic1 PublicationAdd BLAST | 11 | |
Transmembranei | 12 – 36 | HelicalAdd BLAST | 25 | |
Topological domaini | 37 – 52 | Periplasmic1 PublicationAdd BLAST | 16 | |
Transmembranei | 53 – 74 | HelicalAdd BLAST | 22 | |
Topological domaini | 75 – 110 | Cytoplasmic1 PublicationAdd BLAST | 36 | |
Transmembranei | 111 – 134 | HelicalAdd BLAST | 24 | |
Topological domaini | 135 – 150 | Periplasmic1 PublicationAdd BLAST | 16 | |
Transmembranei | 151 – 175 | HelicalAdd BLAST | 25 | |
Topological domaini | 176 – 217 | Cytoplasmic1 PublicationAdd BLAST | 42 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087210 | 1 – 217 | Formate dehydrogenase, nitrate-inducible, cytochrome b556(Fdn) subunitAdd BLAST | 217 |
Proteomic databases
jPOSTi | P0AEK7 |
PaxDbi | P0AEK7 |
PRIDEi | P0AEK7 |
Expressioni
Inductioni
By nitrate under anaerobic conditions.1 Publication
Interactioni
Subunit structurei
Trimer of heterotrimers, consisting of subunits alpha, beta and gamma.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262903, 10 interactors |
ComplexPortali | CPX-1975, Formate dehydrogenase N complex |
IntActi | P0AEK7, 1 interactor |
STRINGi | 511145.b1476 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEK7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEK7 |
Family & Domainsi
Sequence similaritiesi
Belongs to the formate dehydrogenase gamma subunit family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2864, Bacteria |
HOGENOMi | CLU_091368_1_1_6 |
InParanoidi | P0AEK7 |
PhylomeDBi | P0AEK7 |
Family and domain databases
InterProi | View protein in InterPro IPR011577, Cyt_b561_bac/Ni-Hgenase IPR016174, Di-haem_cyt_TM IPR006471, Formate_DH_gsu |
Pfami | View protein in Pfam PF01292, Ni_hydr_CYTB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit |
TIGRFAMsi | TIGR01583, formate-DH-gamm, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEK7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKSKMIVRT KFIDRACHWT VVICFFLVAL SGISFFFPTL QWLTQTFGTP
60 70 80 90 100
QMGRILHPFF GIAIFVALMF MFVRFVHHNI PDKKDIPWLL NIVEVLKGNE
110 120 130 140 150
HKVADVGKYN AGQKMMFWSI MSMIFVLLVT GVIIWRPYFA QYFPMQVVRY
160 170 180 190 200
SLLIHAAAGI ILIHAILIHM YMAFWVKGSI KGMIEGKVSR RWAKKHHPRW
210
YREIEKAEAK KESEEGI
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 160 – 217 | IILIH…SEEGI → YHPDPRHPDPYVYGILGERI D (PubMed:1834669).CuratedAdd BLAST | 58 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75029 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAD13440.1 AP009048 Genomic DNA Translation: BAA15125.1 |
PIRi | G64900, JS0630 |
RefSeqi | NP_415993.1, NC_000913.3 WP_000045648.1, NZ_STEB01000053.1 |
Genome annotation databases
EnsemblBacteriai | AAD13440; AAD13440; b1476 BAA15125; BAA15125; BAA15125 |
GeneIDi | 58391265 946038 |
KEGGi | ecj:JW1472 eco:b1476 |
PATRICi | fig|1411691.4.peg.791 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M75029 Genomic DNA No translation available. U00096 Genomic DNA Translation: AAD13440.1 AP009048 Genomic DNA Translation: BAA15125.1 |
PIRi | G64900, JS0630 |
RefSeqi | NP_415993.1, NC_000913.3 WP_000045648.1, NZ_STEB01000053.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1KQF | X-ray | 1.60 | C | 1-217 | [»] | |
1KQG | X-ray | 2.80 | C | 1-217 | [»] | |
SMRi | P0AEK7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262903, 10 interactors |
ComplexPortali | CPX-1975, Formate dehydrogenase N complex |
IntActi | P0AEK7, 1 interactor |
STRINGi | 511145.b1476 |
Chemistry databases
DrugBanki | DB07918, 2-heptyl-4-hydroxyquinoline N-oxide |
Protein family/group databases
TCDBi | 5.A.3.2.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
jPOSTi | P0AEK7 |
PaxDbi | P0AEK7 |
PRIDEi | P0AEK7 |
Genome annotation databases
EnsemblBacteriai | AAD13440; AAD13440; b1476 BAA15125; BAA15125; BAA15125 |
GeneIDi | 58391265 946038 |
KEGGi | ecj:JW1472 eco:b1476 |
PATRICi | fig|1411691.4.peg.791 |
Organism-specific databases
EchoBASEi | EB1211 |
Phylogenomic databases
eggNOGi | COG2864, Bacteria |
HOGENOMi | CLU_091368_1_1_6 |
InParanoidi | P0AEK7 |
PhylomeDBi | P0AEK7 |
Enzyme and pathway databases
BioCyci | EcoCyc:FDNI-MONOMER MetaCyc:FDNI-MONOMER |
BRENDAi | 1.1.5.6, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AEK7 |
PROi | PR:P0AEK7 |
Family and domain databases
InterProi | View protein in InterPro IPR011577, Cyt_b561_bac/Ni-Hgenase IPR016174, Di-haem_cyt_TM IPR006471, Formate_DH_gsu |
Pfami | View protein in Pfam PF01292, Ni_hydr_CYTB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit |
TIGRFAMsi | TIGR01583, formate-DH-gamm, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FDNI_ECOLI | |
Accessioni | P0AEK7Primary (citable) accession number: P0AEK7 Secondary accession number(s): P24185, P77513 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families