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UniProtKB - P0AEJ6 (EUTB_ECOLI)
Protein
Ethanolamine ammonia-lyase large subunit
Gene
eutB
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds (PubMed:19762342).
Allows this organism to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of external vitamin B12. It is spontaneously inactivated by its substrate and reactivated by EutA (PubMed:15466038).
1 Publication2 PublicationsCaution
In strain MG1655 the eut operon is interrupted by the CPZ-55 prophage, encoding 9 genes situated between eutA and eutB, which are translated in the other direction. CPZ-55 may prevent expression of the eut operon in strain MG1655. Strain W3110 does not have this prophage element and should be able to express the operon.1 Publication
Catalytic activityi
- EC:4.3.1.7UniRule annotation2 Publications
Cofactori
adenosylcob(III)alaminUniRule annotation4 PublicationsNote: Binds 1 AdoCbl between the large and small subunits, with 6 cofactors per holoenzyme.UniRule annotation1 Publication3 Publications
Activity regulationi
Inhibited by 5-adeninylpentylcobalamin (AdePeCbl), a cofactor analog (PubMed:19762342). Irreversibly inhibited during catalysis by cleavage of the Co-C bond of the cobalamin coenzyme (Probable) (PubMed:19762342). Reactivated by EutA, which probably involves an ATP-dependent cobalamin exchange (PubMed:15466038).1 Publication2 Publications
Kineticsi
- KM=8.2 µM for ethanolamine1 Publication
- KM=0.055 µM for adenosylcob(III)alamin1 Publication
: ethanolamine degradation Pathwayi
This protein is involved in the pathway ethanolamine degradation, which is part of Amine and polyamine degradation.UniRule annotationView all proteins of this organism that are known to be involved in the pathway ethanolamine degradation and in Amine and polyamine degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 193 | SubstrateUniRule annotationCombined sources2 Publications | 1 | |
Binding sitei | 194 | Adenosylcob(III)alamin; via carbonyl oxygenUniRule annotationCombined sources1 Publication | 1 | |
Binding sitei | 246 | Adenosylcob(III)alaminUniRule annotationCombined sources2 Publications | 1 | |
Binding sitei | 287 | SubstrateUniRule annotationCombined sources2 Publications | 1 | |
Binding sitei | 295 | Adenosylcob(III)alaminUniRule annotationCombined sourcesCurated | 1 | |
Binding sitei | 362 | SubstrateUniRule annotationCombined sources2 Publications | 1 | |
Binding sitei | 401 | Adenosylcob(III)alamin; via carbonyl oxygenUniRule annotationCombined sources2 Publications | 1 |
GO - Molecular functioni
- cobalamin binding Source: UniProtKB-KW
- ethanolamine ammonia-lyase activity Source: UniProtKB-EC
GO - Biological processi
- cellular amino acid metabolic process Source: InterPro
- ethanolamine catabolic process Source: ComplexPortal
Keywordsi
Molecular function | Lyase |
Ligand | Cobalamin, Cobalt |
Enzyme and pathway databases
BioCyci | EcoCyc:EUTB-MONOMER |
UniPathwayi | UPA00560 |
Names & Taxonomyi
Protein namesi | Recommended name: Ethanolamine ammonia-lyase large subunit1 PublicationUniRule annotation (EC:4.3.1.7UniRule annotation2 Publications)Short name: EAL large subunit1 PublicationUniRule annotation Alternative name(s): Ethanolamine ammonia-lyase alpha subunit1 Publication Ethanolamine ammonia-lyase heavy chain Ethanolamine deaminase large subunit1 Publication |
Gene namesi | Name:eutBUniRule annotation Ordered Locus Names:b2441, JW2434 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Bacterial microcompartment UniRule annotation
Cytosol
- cytosol Source: EcoCyc
Other locations
- ethanolamine ammonia-lyase complex Source: EcoCyc
Keywords - Cellular componenti
Bacterial microcompartmentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087081 | 1 – 453 | Ethanolamine ammonia-lyase large subunitAdd BLAST | 453 |
Proteomic databases
jPOSTi | P0AEJ6 |
PaxDbi | P0AEJ6 |
PRIDEi | P0AEJ6 |
Expressioni
Inductioni
When grown in ethanolamine and adenosylcobalamin (AdoCbl) (at protein level). Note this experiment was done in strain JM109, which expresses the eut operon.1 Publication
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 4260578, 10 interactors |
ComplexPortali | CPX-2313, Ethanolamine ammonia-lyase complex |
IntActi | P0AEJ6, 1 interactor |
STRINGi | 511145.b2441 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEJ6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEJ6 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 160 – 162 | Substrate bindingUniRule annotationCombined sources2 Publications | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG4303, Bacteria |
HOGENOMi | CLU_048555_0_0_6 |
InParanoidi | P0AEJ6 |
PhylomeDBi | P0AEJ6 |
Family and domain databases
Gene3Di | 1.10.220.70, 1 hit 2.30.170.30, 1 hit 3.20.20.70, 1 hit |
HAMAPi | MF_00861, EutB, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR010628, EutB IPR044939, EutB_dom_2_sf IPR044941, EutB_N_sf |
PANTHERi | PTHR39329, PTHR39329, 1 hit |
Pfami | View protein in Pfam PF06751, EutB, 1 hit |
PIRSFi | PIRSF018788, EutB, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEJ6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKLKTTLFGN VYQFKDVKEV LAKANELRSG DVLAGVAAAS SQERVAAKQV
60 70 80 90 100
LSEMTVADIR NNPVIAYEDD CVTRLIQDDV NETAYNQIKN WSISELREYV
110 120 130 140 150
LSDETSVDDI AFTRKGLTSE VVAAVAKICS NADLIYGAKK MPVIKKANTT
160 170 180 190 200
IGIPGTFSAR LQPNDTRDDV QSIAAQIYEG LSFGVGDAVI GVNPVTDDVE
210 220 230 240 250
NLSRVLDTIY GVIDKFNIPT QGCVLAHVTT QIEAIRRGAP GGLIFQSICG
260 270 280 290 300
SEKGLKEFGV ELAMLDEARA VGAEFNRIAG ENCLYFETGQ GSALSAGANF
310 320 330 340 350
GADQVTMEAR NYGLARHYDP FIVNTVVGFI GPEYLYNDRQ IIRAGLEDHF
360 370 380 390 400
MGKLSGISMG CDCCYTNHAD ADQNLNENLM ILLATAGCNY IMGMPLGDDI
410 420 430 440 450
MLNYQTTAFH DTATVRQLLN LRPSPEFERW LESMGIMANG RLTKRAGDPS
LFF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75494.2 AP009048 Genomic DNA Translation: BAA16323.1 |
PIRi | H65018 |
RefSeqi | NP_416936.4, NC_000913.3 WP_000769961.1, NZ_SSUR01000044.1 |
Genome annotation databases
EnsemblBacteriai | AAC75494; AAC75494; b2441 BAA16323; BAA16323; BAA16323 |
GeneIDi | 66673689 946924 |
KEGGi | ecj:JW2434 eco:b2441 |
PATRICi | fig|1411691.4.peg.4290 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75494.2 AP009048 Genomic DNA Translation: BAA16323.1 |
PIRi | H65018 |
RefSeqi | NP_416936.4, NC_000913.3 WP_000769961.1, NZ_SSUR01000044.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3ABO | X-ray | 2.10 | A/C | 1-453 | [»] | |
3ABQ | X-ray | 2.05 | A/C | 1-453 | [»] | |
3ABR | X-ray | 2.10 | A/C | 1-453 | [»] | |
3ABS | X-ray | 2.25 | A/C | 1-453 | [»] | |
3ANY | X-ray | 2.10 | A/C | 1-453 | [»] | |
3AO0 | X-ray | 2.25 | A/C | 1-453 | [»] | |
5YSN | X-ray | 2.00 | A/C | 1-453 | [»] | |
5YSR | X-ray | 2.05 | A/C | 1-453 | [»] | |
SMRi | P0AEJ6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260578, 10 interactors |
ComplexPortali | CPX-2313, Ethanolamine ammonia-lyase complex |
IntActi | P0AEJ6, 1 interactor |
STRINGi | 511145.b2441 |
Proteomic databases
jPOSTi | P0AEJ6 |
PaxDbi | P0AEJ6 |
PRIDEi | P0AEJ6 |
Protocols and materials databases
DNASUi | 946924 |
Genome annotation databases
EnsemblBacteriai | AAC75494; AAC75494; b2441 BAA16323; BAA16323; BAA16323 |
GeneIDi | 66673689 946924 |
KEGGi | ecj:JW2434 eco:b2441 |
PATRICi | fig|1411691.4.peg.4290 |
Organism-specific databases
EchoBASEi | EB4299 |
Phylogenomic databases
eggNOGi | COG4303, Bacteria |
HOGENOMi | CLU_048555_0_0_6 |
InParanoidi | P0AEJ6 |
PhylomeDBi | P0AEJ6 |
Enzyme and pathway databases
UniPathwayi | UPA00560 |
BioCyci | EcoCyc:EUTB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AEJ6 |
PROi | PR:P0AEJ6 |
Family and domain databases
Gene3Di | 1.10.220.70, 1 hit 2.30.170.30, 1 hit 3.20.20.70, 1 hit |
HAMAPi | MF_00861, EutB, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR010628, EutB IPR044939, EutB_dom_2_sf IPR044941, EutB_N_sf |
PANTHERi | PTHR39329, PTHR39329, 1 hit |
Pfami | View protein in Pfam PF06751, EutB, 1 hit |
PIRSFi | PIRSF018788, EutB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EUTB_ECOLI | |
Accessioni | P0AEJ6Primary (citable) accession number: P0AEJ6 Secondary accession number(s): P19635, P78094, P78300 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families