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Protein

Osmolarity sensor protein EnvZ

Gene

envZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system EnvZ/OmpR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates OmpR in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Activity regulationi

Activity is modulated by MzrA.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei347ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi373 – 387ATPAdd BLAST15

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • phosphoprotein phosphatase activity Source: EcoCyc
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ENVZ-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Osmolarity sensor protein EnvZ (EC:2.7.13.3)
Gene namesi
Name:envZ
Synonyms:ompB, perA, tpo
Ordered Locus Names:b3404, JW3367
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10269 envZ

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 15CytoplasmicCuratedAdd BLAST15
Transmembranei16 – 35HelicalCuratedAdd BLAST20
Topological domaini36 – 158PeriplasmicCuratedAdd BLAST123
Transmembranei159 – 179HelicalCuratedAdd BLAST21
Topological domaini180 – 450CytoplasmicCuratedAdd BLAST271

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi193A → L: Promotes the formation of alpha-helical secondary structure of the HAMP domain. 1 Publication1
Mutagenesisi193A → V: No effect. 1 Publication1
Mutagenesisi347N → D: Loss of ATP binding; loss of autophosphorylation. 1 Publication1

Chemistry databases

DrugBankiDB04395 Phosphoaminophosphonic Acid-Adenylate Ester

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747591 – 450Osmolarity sensor protein EnvZAdd BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei243Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1 Publication1

Post-translational modificationi

Autophosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0AEJ4
PRIDEiP0AEJ4

PTM databases

iPTMnetiP0AEJ4

Interactioni

Subunit structurei

Homodimer. Interacts with MzrA.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4261266, 47 interactors
DIPiDIP-48357N
IntActiP0AEJ4, 5 interactors
MINTiP0AEJ4
STRINGi316385.ECDH10B_3579

Structurei

Secondary structure

1450
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AEJ4
SMRiP0AEJ4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEJ4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini180 – 232HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini240 – 440Histidine kinasePROSITE-ProRule annotationAdd BLAST201

Domaini

The HAMP domain by itself is intrinsically disordered.1 Publication

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105E0F Bacteria
ENOG410XTWD LUCA
HOGENOMiHOG000218774
InParanoidiP0AEJ4
KOiK07638
OMAiWIRPPQA
PhylomeDBiP0AEJ4

Family and domain databases

CDDicd06225 HAMP, 1 hit
cd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003660 HAMP_dom
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF00672 HAMP, 1 hit
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00304 HAMP, 1 hit
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50885 HAMP, 1 hit
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEJ4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRRLRFSPRS SFARTLLLIV TLLFASLVTT YLVVLNFAIL PSLQQFNKVL
60 70 80 90 100
AYEVRMLMTD KLQLEDGTQL VVPPAFRREI YRELGISLYS NEAAEEAGLR
110 120 130 140 150
WAQHYEFLSH QMAQQLGGPT EVRVEVNKSS PVVWLKTWLS PNIWVRVPLT
160 170 180 190 200
EIHQGDFSPL FRYTLAIMLL AIGGAWLFIR IQNRPLVDLE HAALQVGKGI
210 220 230 240 250
IPPPLREYGA SEVRSVTRAF NHMAAGVKQL ADDRTLLMAG VSHDLRTPLT
260 270 280 290 300
RIRLATEMMS EQDGYLAESI NKDIEECNAI IEQFIDYLRT GQEMPMEMAD
310 320 330 340 350
LNAVLGEVIA AESGYEREIE TALYPGSIEV KMHPLSIKRA VANMVVNAAR
360 370 380 390 400
YGNGWIKVSS GTEPNRAWFQ VEDDGPGIAP EQRKHLFQPF VRGDSARTIS
410 420 430 440 450
GTGLGLAIVQ RIVDNHNGML ELGTSERGGL SIRAWLPVPV TRAQGTTKEG
Length:450
Mass (Da):50,334
Last modified:December 20, 2005 - v1
Checksum:iD58444D038722146
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01656 Unassigned RNA Translation: AAA16242.1
U18997 Genomic DNA Translation: AAA58201.1
U00096 Genomic DNA Translation: AAC76429.1
AP009048 Genomic DNA Translation: BAE77887.1
PIRiB25024 MMECZB
RefSeqiNP_417863.1, NC_000913.3
WP_001253696.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76429; AAC76429; b3404
BAE77887; BAE77887; BAE77887
GeneIDi947272
KEGGiecj:JW3367
eco:b3404
PATRICifig|1411691.4.peg.3325

Entry informationi

Entry nameiENVZ_ECOLI
AccessioniPrimary (citable) accession number: P0AEJ4
Secondary accession number(s): P02933, Q2M769
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: December 20, 2005
Last modified: September 12, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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