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Protein

Isochorismate synthase EntC

Gene

entC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the siderophore enterobactin (macrocyclic trimeric lactone of N-(2,3-dihydroxybenzoyl)-serine). Catalyzes the reversible conversion of chorismate to isochorismate.6 Publications

Catalytic activityi

Chorismate = isochorismate.3 Publications

Cofactori

Mg2+1 Publication

Kineticsi

Kcat is 290 min(-1) for mutase activity with chorismate. Kcat is 173 min(-1) for mutase activity with chorismate (at pH 7.8 and 37 degrees Celsius). Kcat is 108 min(-1) for mutase activity with isochorismate (at pH 7.8 and 37 degrees Celsius). Kcat is 37 min(-1) for mutase activity with chorismate.3 Publications
  1. KM=5 µM for isochorismate (at pH 7.8 and 37 degrees Celsius)1 Publication
  2. KM=7 µM for chorismate1 Publication
  3. KM=14 µM for chorismate (at pH 7.8 and 37 degrees Celsius)1 Publication
  4. KM=41 µM for chorismate1 Publication

    Pathwayi: enterobactin biosynthesis

    This protein is involved in the pathway enterobactin biosynthesis, which is part of Siderophore biosynthesis.Curated
    View all proteins of this organism that are known to be involved in the pathway enterobactin biosynthesis and in Siderophore biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi140Magnesium; via carbonyl oxygen1 Publication1
    Metal bindingi142Magnesium; via carbonyl oxygen1 Publication1
    Metal bindingi145Magnesium; via carbonyl oxygen1 Publication1
    Metal bindingi146Magnesium1 Publication1
    Active sitei147Proton acceptor1 Publication1
    Active sitei197Proton donor1 Publication1
    Metal bindingi241Magnesium1 Publication1
    Binding sitei241Substrate1 Publication1
    Binding sitei303Substrate; via carbonyl oxygen1 Publication1
    Binding sitei347Substrate1 Publication1
    Binding sitei361Substrate; via amide nitrogen1 Publication1
    Metal bindingi376Magnesium1 Publication1

    GO - Molecular functioni

    • isochorismate synthase activity Source: EcoCyc
    • magnesium ion binding Source: UniProtKB

    GO - Biological processi

    • enterobactin biosynthetic process Source: EcoCyc
    • salicylic acid biosynthetic process Source: GO_Central

    Keywordsi

    Molecular functionIsomerase
    Biological processEnterobactin biosynthesis
    LigandMagnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ENTC-MONOMER
    MetaCyc:ENTC-MONOMER
    BRENDAi5.4.4.2 2026
    SABIO-RKiP0AEJ2
    UniPathwayi
    UPA00017

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isochorismate synthase EntC1 Publication (EC:5.4.4.23 Publications)
    Alternative name(s):
    Isochorismate mutaseCurated
    Gene namesi
    Name:entC1 Publication
    Ordered Locus Names:b0593, JW0585
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10261 entC

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene are unable to produce enterobactin and are hypersensitive to the antimicrobial peptide wrwycr.2 Publications

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi303A → T: Loss of mutase activity. 1 Publication1
    Mutagenesisi304L → A: Loss of mutase activity. 1 Publication1
    Mutagenesisi327F → Y: Loss of mutase activity. 1 Publication1
    Mutagenesisi346I → L: Loss of mutase activity. 1 Publication1
    Mutagenesisi359F → Q: Loss of mutase activity. 1 Publication1

    Chemistry databases

    ChEMBLiCHEMBL1075176

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001541441 – 391Isochorismate synthase EntCAdd BLAST391

    Proteomic databases

    PaxDbiP0AEJ2
    PRIDEiP0AEJ2

    Expressioni

    Inductioni

    Under conditions of iron deficiency and by the fur protein.2 Publications

    Interactioni

    Subunit structurei

    Monomer.2 Publications

    Protein-protein interaction databases

    BioGridi4260986, 160 interactors
    DIPiDIP-47970N
    IntActiP0AEJ2, 9 interactors
    STRINGi316385.ECDH10B_0661

    Chemistry databases

    BindingDBiP0AEJ2

    Structurei

    Secondary structure

    1391
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliP0AEJ2
    SMRiP0AEJ2
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP0AEJ2

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni214 – 215Substrate1 Publication2

    Sequence similaritiesi

    Belongs to the isochorismate synthase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105E4F Bacteria
    COG1169 LUCA
    HOGENOMiHOG000028185
    InParanoidiP0AEJ2
    KOiK02361
    PhylomeDBiP0AEJ2

    Family and domain databases

    Gene3Di3.60.120.10, 1 hit
    InterProiView protein in InterPro
    IPR005801 ADC_synthase
    IPR015890 Chorismate_C
    IPR004561 IsoChor_synthase
    PfamiView protein in Pfam
    PF00425 Chorismate_bind, 1 hit
    SUPFAMiSSF56322 SSF56322, 1 hit
    TIGRFAMsiTIGR00543 isochor_syn, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0AEJ2-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MDTSLAEEVQ QTMATLAPNR FFFMSPYRSF TTSGCFARFD EPAVNGDSPD
    60 70 80 90 100
    SPFQQKLAAL FADAKAQGIK NPVMVGAIPF DPRQPSSLYI PESWQSFSRQ
    110 120 130 140 150
    EKQASARRFT RSQSLNVVER QAIPEQTTFE QMVARAAALT ATPQVDKVVL
    160 170 180 190 200
    SRLIDITTDA AIDSGVLLER LIAQNPVSYN FHVPLADGGV LLGASPELLL
    210 220 230 240 250
    RKDGERFSSI PLAGSARRQP DEVLDREAGN RLLASEKDRH EHELVTQAMK
    260 270 280 290 300
    EVLRERSSEL HVPSSPQLIT TPTLWHLATP FEGKANSQEN ALTLACLLHP
    310 320 330 340 350
    TPALSGFPHQ AATQVIAELE PFDRELFGGI VGWCDSEGNG EWVVTIRCAK
    360 370 380 390
    LRENQVRLFA GAGIVPASSP LGEWRETGVK LSTMLNVFGL H
    Length:391
    Mass (Da):42,932
    Last modified:December 20, 2005 - v1
    Checksum:i62882569DFC41AC4
    GO

    Sequence cautioni

    The sequence AAB40793 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti305 – 306SG → TA in AAA18491 (PubMed:2110093).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M36700 Genomic DNA Translation: AAA18491.1
    M24142 Unassigned DNA Translation: AAA16100.1
    U82598 Genomic DNA Translation: AAB40793.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73694.1
    AP009048 Genomic DNA Translation: BAE76348.1
    X53274 Genomic DNA Translation: CAA37371.1
    PIRiJT0497 SYECIK
    RefSeqiNP_415125.1, NC_000913.3
    WP_000381303.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73694; AAC73694; b0593
    BAE76348; BAE76348; BAE76348
    GeneIDi945511
    KEGGiecj:JW0585
    eco:b0593
    PATRICifig|1411691.4.peg.1676

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M36700 Genomic DNA Translation: AAA18491.1
    M24142 Unassigned DNA Translation: AAA16100.1
    U82598 Genomic DNA Translation: AAB40793.1 Different initiation.
    U00096 Genomic DNA Translation: AAC73694.1
    AP009048 Genomic DNA Translation: BAE76348.1
    X53274 Genomic DNA Translation: CAA37371.1
    PIRiJT0497 SYECIK
    RefSeqiNP_415125.1, NC_000913.3
    WP_000381303.1, NZ_LN832404.1

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3HWOX-ray2.30A/B1-391[»]
    5JXZX-ray1.88A/B1-391[»]
    5JY4X-ray2.11A/B1-391[»]
    5JY8X-ray2.94A/B1-391[»]
    5JZDX-ray2.30A/B1-391[»]
    ProteinModelPortaliP0AEJ2
    SMRiP0AEJ2
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4260986, 160 interactors
    DIPiDIP-47970N
    IntActiP0AEJ2, 9 interactors
    STRINGi316385.ECDH10B_0661

    Chemistry databases

    BindingDBiP0AEJ2
    ChEMBLiCHEMBL1075176

    Proteomic databases

    PaxDbiP0AEJ2
    PRIDEiP0AEJ2

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73694; AAC73694; b0593
    BAE76348; BAE76348; BAE76348
    GeneIDi945511
    KEGGiecj:JW0585
    eco:b0593
    PATRICifig|1411691.4.peg.1676

    Organism-specific databases

    EchoBASEiEB0257
    EcoGeneiEG10261 entC

    Phylogenomic databases

    eggNOGiENOG4105E4F Bacteria
    COG1169 LUCA
    HOGENOMiHOG000028185
    InParanoidiP0AEJ2
    KOiK02361
    PhylomeDBiP0AEJ2

    Enzyme and pathway databases

    UniPathwayi
    UPA00017

    BioCyciEcoCyc:ENTC-MONOMER
    MetaCyc:ENTC-MONOMER
    BRENDAi5.4.4.2 2026
    SABIO-RKiP0AEJ2

    Miscellaneous databases

    EvolutionaryTraceiP0AEJ2
    PROiPR:P0AEJ2

    Family and domain databases

    Gene3Di3.60.120.10, 1 hit
    InterProiView protein in InterPro
    IPR005801 ADC_synthase
    IPR015890 Chorismate_C
    IPR004561 IsoChor_synthase
    PfamiView protein in Pfam
    PF00425 Chorismate_bind, 1 hit
    SUPFAMiSSF56322 SSF56322, 1 hit
    TIGRFAMsiTIGR00543 isochor_syn, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiENTC_ECOLI
    AccessioniPrimary (citable) accession number: P0AEJ2
    Secondary accession number(s): P10377, P77099, Q2MBK8
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: December 20, 2005
    Last modified: November 7, 2018
    This is version 96 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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