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UniProtKB - P0AEJ1 (EMRB_ECO57)
Protein
Multidrug export protein EmrB
Gene
emrB
Organism
Escherichia coli O157:H7
Status
Functioni
Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics.
By similarityGO - Molecular functioni
- transmembrane transporter activity Source: InterPro
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Biological process | Antibiotic resistance, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug export protein EmrB |
Gene namesi | Name:emrB Ordered Locus Names:Z3987, ECs3548 |
Organismi | Escherichia coli O157:H7 |
Taxonomic identifieri | 83334 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane By similarity; Multi-pass membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 38 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 39 – 51 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 52 – 72 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 73 – 81 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 82 – 100 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 101 – 109 | ExtracellularSequence analysis | 9 | |
Transmembranei | 110 – 128 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 129 – 136 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 137 – 159 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 160 – 164 | ExtracellularSequence analysis | 5 | |
Transmembranei | 165 – 189 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 190 – 202 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 203 – 223 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 224 – 233 | ExtracellularSequence analysis | 10 | |
Transmembranei | 234 – 249 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 250 – 271 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 272 – 295 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 296 – 305 | ExtracellularSequence analysis | 10 | |
Transmembranei | 306 – 329 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 330 – 335 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 336 – 355 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 356 – 363 | ExtracellularSequence analysis | 8 | |
Transmembranei | 364 – 387 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 388 – 407 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 408 – 428 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 429 – 481 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 482 – 504 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 505 – 512 | CytoplasmicSequence analysis | 8 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173323 | 1 – 512 | Multidrug export protein EmrBAdd BLAST | 512 |
Interactioni
Subunit structurei
Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.
Interacts with EmrA (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 155864.EDL933_3853 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
OMAi | GNICYGA |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEJ1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ
60 70 80 90 100
GTWVITSFGV ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS
110 120 130 140 150
SLNMLIFFRV IQGIVAGPLI PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA
160 170 180 190 200
PICGPILGGY ISDNYHWGWI FFINVPIGVA VVLMTLQTLR GRETRTERRR
210 220 230 240 250
IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV AVVAICFLIV
260 270 280 290 300
WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG
310 320 330 340 350
YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW
360 370 380 390 400
RAYTFEPGMD FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS
410 420 430 440 450
LSNFTRTLAG SIGTSITTTM WTNRESMHHA QLTESVNPFN PNAQAMYSQL
460 470 480 490 500
EGLGMTQQQA SGWIAQQITN QGLIISANEI FWMSAGIFLV LLGLVWFAKP
510
PFGAGGGGGG AH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57795.1 BA000007 Genomic DNA Translation: BAB36971.1 |
PIRi | D91072 |
RefSeqi | NP_311575.1, NC_002695.1 WP_001295176.1, NZ_SWKA01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAG57795; AAG57795; Z3987 BAB36971; BAB36971; ECs_3548 |
GeneIDi | 66673445 914736 |
KEGGi | ece:Z3987 ecs:ECs3548 |
PATRICi | fig|386585.9.peg.3704 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005174 Genomic DNA Translation: AAG57795.1 BA000007 Genomic DNA Translation: BAB36971.1 |
PIRi | D91072 |
RefSeqi | NP_311575.1, NC_002695.1 WP_001295176.1, NZ_SWKA01000005.1 |
3D structure databases
AlphaFoldDBi | P0AEJ1 |
SMRi | P0AEJ1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 155864.EDL933_3853 |
Genome annotation databases
EnsemblBacteriai | AAG57795; AAG57795; Z3987 BAB36971; BAB36971; ECs_3548 |
GeneIDi | 66673445 914736 |
KEGGi | ece:Z3987 ecs:ECs3548 |
PATRICi | fig|386585.9.peg.3704 |
Phylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
OMAi | GNICYGA |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EMRB_ECO57 | |
Accessioni | P0AEJ1Primary (citable) accession number: P0AEJ1 Secondary accession number(s): P27304, P77725 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | May 25, 2022 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families