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UniProtKB - P0AEJ0 (EMRB_ECOLI)

Entry version 114 (23 Feb 2022)
Sequence version 1 (20 Dec 2005)
Previous versions | rss
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Protein

Multidrug export protein EmrB

Gene

emrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics such as CCCP, FCCP, 2,4-dinitrophenol and nalidixic acid.

2 Publications

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAntibiotic resistance, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EMRB-MONOMER

Protein family/group databases

Transport Classification Database

More...TCDBi		2.A.1.3.2, the major facilitator superfamily (mfs)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Multidrug export protein EmrB
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:emrB
Ordered Locus Names:b2686, JW2661
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei13 – 38HelicalSequence analysisAdd BLAST26
Topological domaini39 – 51ExtracellularSequence analysisAdd BLAST13
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 81CytoplasmicSequence analysis9
Transmembranei82 – 100HelicalSequence analysisAdd BLAST19
Topological domaini101 – 109ExtracellularSequence analysis9
Transmembranei110 – 128HelicalSequence analysisAdd BLAST19
Topological domaini129 – 136CytoplasmicSequence analysis8
Transmembranei137 – 159HelicalSequence analysisAdd BLAST23
Topological domaini160 – 164ExtracellularSequence analysis5
Transmembranei165 – 189HelicalSequence analysisAdd BLAST25
Topological domaini190 – 202CytoplasmicSequence analysisAdd BLAST13
Transmembranei203 – 223HelicalSequence analysisAdd BLAST21
Topological domaini224 – 233ExtracellularSequence analysis10
Transmembranei234 – 249HelicalSequence analysisAdd BLAST16
Topological domaini250 – 271CytoplasmicSequence analysisAdd BLAST22
Transmembranei272 – 295HelicalSequence analysisAdd BLAST24
Topological domaini296 – 305ExtracellularSequence analysis10
Transmembranei306 – 329HelicalSequence analysisAdd BLAST24
Topological domaini330 – 335CytoplasmicSequence analysis6
Transmembranei336 – 355HelicalSequence analysisAdd BLAST20
Topological domaini356 – 363ExtracellularSequence analysis8
Transmembranei364 – 387HelicalSequence analysisAdd BLAST24
Topological domaini388 – 407CytoplasmicSequence analysisAdd BLAST20
Transmembranei408 – 428HelicalSequence analysisAdd BLAST21
Topological domaini429 – 481ExtracellularSequence analysisAdd BLAST53
Transmembranei482 – 504HelicalSequence analysisAdd BLAST23
Topological domaini505 – 512CytoplasmicSequence analysis8

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001733221 – 512Multidrug export protein EmrBAdd BLAST512

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AEJ0

PRoteomics IDEntifications database

More...PRIDEi		P0AEJ0

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.

Interacts with EmrA. EmrAB complex forms a dimer in vitro.

2 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4263179, 251 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-4268, EmrAB-TolC multidrug efflux transport system

Protein interaction database and analysis system

More...IntActi		P0AEJ0, 1 interactor

STRING: functional protein association networks

More...STRINGi		511145.b2686

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AEJ0

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the major facilitator superfamily. EmrB family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG2814, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_000960_28_0_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AEJ0

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AEJ0

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1250.20, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR004638, EmrB-like
IPR011701, MFS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF07690, MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF103473, SSF103473, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00711, efflux_EmrB, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50850, MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AEJ0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ 
        60         70         80         90        100
GTWVITSFGV ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS 
       110        120        130        140        150
SLNMLIFFRV IQGIVAGPLI PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA 
       160        170        180        190        200
PICGPILGGY ISDNYHWGWI FFINVPIGVA VVLMTLQTLR GRETRTERRR 
       210        220        230        240        250
IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV AVVAICFLIV 
       260        270        280        290        300
WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG 
       310        320        330        340        350
YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW 
       360        370        380        390        400
RAYTFEPGMD FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS 
       410        420        430        440        450
LSNFTRTLAG SIGTSITTTM WTNRESMHHA QLTESVNPFN PNAQAMYSQL 
       460        470        480        490        500
EGLGMTQQQA SGWIAQQITN QGLIISANEI FWMSAGIFLV LLGLVWFAKP 
       510 
PFGAGGGGGG AH
Length:512
Mass (Da):55,624
Last modified:December 20, 2005 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i070334E68B303EFF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti325G → A in AAA23725 (PubMed:1409590).Curated1
Sequence conflicti501P → A in AAA23725 (PubMed:1409590).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
M86657 Genomic DNA Translation: AAA23725.1
U00096 Genomic DNA Translation: AAC75733.1
AP009048 Genomic DNA Translation: BAA16548.1

Protein sequence database of the Protein Information Resource

More...PIRi		G65048

NCBI Reference Sequences

More...RefSeqi		NP_417171.1, NC_000913.3
WP_001295176.1, NZ_STEB01000042.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC75733; AAC75733; b2686
BAA16548; BAA16548; BAA16548

Database of genes from NCBI RefSeq genomes

More...GeneIDi		66673445
947167

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW2661
eco:b2686

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.4053

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86657 Genomic DNA Translation: AAA23725.1
U00096 Genomic DNA Translation: AAC75733.1
AP009048 Genomic DNA Translation: BAA16548.1
PIRiG65048
RefSeqiNP_417171.1, NC_000913.3
WP_001295176.1, NZ_STEB01000042.1

3D structure databases

SMRiP0AEJ0
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi4263179, 251 interactors
ComplexPortaliCPX-4268, EmrAB-TolC multidrug efflux transport system
IntActiP0AEJ0, 1 interactor
STRINGi511145.b2686

Protein family/group databases

TCDBi2.A.1.3.2, the major facilitator superfamily (mfs)

Proteomic databases

PaxDbiP0AEJ0
PRIDEiP0AEJ0

Genome annotation databases

EnsemblBacteriaiAAC75733; AAC75733; b2686
BAA16548; BAA16548; BAA16548
GeneIDi66673445
947167
KEGGiecj:JW2661
eco:b2686
PATRICifig|1411691.4.peg.4053

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB1409

Phylogenomic databases

eggNOGiCOG2814, Bacteria
HOGENOMiCLU_000960_28_0_6
InParanoidiP0AEJ0
PhylomeDBiP0AEJ0

Enzyme and pathway databases

BioCyciEcoCyc:EMRB-MONOMER

Miscellaneous databases

Protein Ontology

More...PROi		PR:P0AEJ0

Family and domain databases

Gene3Di1.20.1250.20, 1 hit
InterProiView protein in InterPro
IPR004638, EmrB-like
IPR011701, MFS
IPR020846, MFS_dom
IPR036259, MFS_trans_sf
PfamiView protein in Pfam
PF07690, MFS_1, 1 hit
SUPFAMiSSF103473, SSF103473, 1 hit
TIGRFAMsiTIGR00711, efflux_EmrB, 1 hit
PROSITEiView protein in PROSITE
PS50850, MFS, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiEMRB_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AEJ0
Secondary accession number(s): P27304, P77725
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: February 23, 2022
This is version 114 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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