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UniProtKB - P0AEJ0 (EMRB_ECOLI)
Protein
Multidrug export protein EmrB
Gene
emrB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the tripartite efflux system EmrAB-TolC, which confers resistance to antibiotics such as CCCP, FCCP, 2,4-dinitrophenol and nalidixic acid.
2 PublicationsGO - Molecular functioni
- transmembrane transporter activity Source: GO_Central
GO - Biological processi
- proton transmembrane transport Source: GOC
- response to antibiotic Source: UniProtKB-KW
- response to toxic substance Source: EcoCyc
- transmembrane transport Source: GO_Central
- xenobiotic detoxification by transmembrane export across the plasma membrane Source: EcoCyc
Keywordsi
Biological process | Antibiotic resistance, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:EMRB-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.3.2, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: Multidrug export protein EmrB |
Gene namesi | Name:emrB Ordered Locus Names:b2686, JW2661 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Plasma Membrane
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 12 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 13 – 38 | HelicalSequence analysisAdd BLAST | 26 | |
Topological domaini | 39 – 51 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 52 – 72 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 73 – 81 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 82 – 100 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 101 – 109 | ExtracellularSequence analysis | 9 | |
Transmembranei | 110 – 128 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 129 – 136 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 137 – 159 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 160 – 164 | ExtracellularSequence analysis | 5 | |
Transmembranei | 165 – 189 | HelicalSequence analysisAdd BLAST | 25 | |
Topological domaini | 190 – 202 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 203 – 223 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 224 – 233 | ExtracellularSequence analysis | 10 | |
Transmembranei | 234 – 249 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 250 – 271 | CytoplasmicSequence analysisAdd BLAST | 22 | |
Transmembranei | 272 – 295 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 296 – 305 | ExtracellularSequence analysis | 10 | |
Transmembranei | 306 – 329 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 330 – 335 | CytoplasmicSequence analysis | 6 | |
Transmembranei | 336 – 355 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 356 – 363 | ExtracellularSequence analysis | 8 | |
Transmembranei | 364 – 387 | HelicalSequence analysisAdd BLAST | 24 | |
Topological domaini | 388 – 407 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 408 – 428 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 429 – 481 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 482 – 504 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 505 – 512 | CytoplasmicSequence analysis | 8 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000173322 | 1 – 512 | Multidrug export protein EmrBAdd BLAST | 512 |
Proteomic databases
PaxDbi | P0AEJ0 |
PRIDEi | P0AEJ0 |
Interactioni
Subunit structurei
Part of the tripartite efflux system EmrAB-TolC, which is composed of an inner membrane transporter, EmrB, a periplasmic membrane fusion protein, EmrA, and an outer membrane component, TolC. The complex forms a large protein conduit and can translocate molecules across both the inner and outer membranes.
Interacts with EmrA. EmrAB complex forms a dimer in vitro.
2 PublicationsBinary interactionsi
P0AEJ0
With | #Exp. | IntAct |
---|---|---|
emrA [P27303] | 2 | EBI-21407519,EBI-1119547 |
Protein-protein interaction databases
BioGRIDi | 4263179, 251 interactors |
ComplexPortali | CPX-4268, EmrAB-TolC multidrug efflux transport system |
IntActi | P0AEJ0, 1 interactor |
STRINGi | 511145.b2686 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
InParanoidi | P0AEJ0 |
PhylomeDBi | P0AEJ0 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEJ0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQQQKPLEGA QLVIMTIALS LATFMQVLDS TIANVAIPTI AGNLGSSLSQ
60 70 80 90 100
GTWVITSFGV ANAISIPLTG WLAKRVGEVK LFLWSTIAFA IASWACGVSS
110 120 130 140 150
SLNMLIFFRV IQGIVAGPLI PLSQSLLLNN YPPAKRSIAL ALWSMTVIVA
160 170 180 190 200
PICGPILGGY ISDNYHWGWI FFINVPIGVA VVLMTLQTLR GRETRTERRR
210 220 230 240 250
IDAVGLALLV IGIGSLQIML DRGKELDWFS SQEIIILTVV AVVAICFLIV
260 270 280 290 300
WELTDDNPIV DLSLFKSRNF TIGCLCISLA YMLYFGAIVL LPQLLQEVYG
310 320 330 340 350
YTATWAGLAS APVGIIPVIL SPIIGRFAHK LDMRRLVTFS FIMYAVCFYW
360 370 380 390 400
RAYTFEPGMD FGASAWPQFI QGFAVACFFM PLTTITLSGL PPERLAAASS
410 420 430 440 450
LSNFTRTLAG SIGTSITTTM WTNRESMHHA QLTESVNPFN PNAQAMYSQL
460 470 480 490 500
EGLGMTQQQA SGWIAQQITN QGLIISANEI FWMSAGIFLV LLGLVWFAKP
510
PFGAGGGGGG AH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 325 | G → A in AAA23725 (PubMed:1409590).Curated | 1 | |
Sequence conflicti | 501 | P → A in AAA23725 (PubMed:1409590).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86657 Genomic DNA Translation: AAA23725.1 U00096 Genomic DNA Translation: AAC75733.1 AP009048 Genomic DNA Translation: BAA16548.1 |
PIRi | G65048 |
RefSeqi | NP_417171.1, NC_000913.3 WP_001295176.1, NZ_STEB01000042.1 |
Genome annotation databases
EnsemblBacteriai | AAC75733; AAC75733; b2686 BAA16548; BAA16548; BAA16548 |
GeneIDi | 66673445 947167 |
KEGGi | ecj:JW2661 eco:b2686 |
PATRICi | fig|1411691.4.peg.4053 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M86657 Genomic DNA Translation: AAA23725.1 U00096 Genomic DNA Translation: AAC75733.1 AP009048 Genomic DNA Translation: BAA16548.1 |
PIRi | G65048 |
RefSeqi | NP_417171.1, NC_000913.3 WP_001295176.1, NZ_STEB01000042.1 |
3D structure databases
SMRi | P0AEJ0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263179, 251 interactors |
ComplexPortali | CPX-4268, EmrAB-TolC multidrug efflux transport system |
IntActi | P0AEJ0, 1 interactor |
STRINGi | 511145.b2686 |
Protein family/group databases
TCDBi | 2.A.1.3.2, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P0AEJ0 |
PRIDEi | P0AEJ0 |
Genome annotation databases
EnsemblBacteriai | AAC75733; AAC75733; b2686 BAA16548; BAA16548; BAA16548 |
GeneIDi | 66673445 947167 |
KEGGi | ecj:JW2661 eco:b2686 |
PATRICi | fig|1411691.4.peg.4053 |
Organism-specific databases
EchoBASEi | EB1409 |
Phylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_000960_28_0_6 |
InParanoidi | P0AEJ0 |
PhylomeDBi | P0AEJ0 |
Enzyme and pathway databases
BioCyci | EcoCyc:EMRB-MONOMER |
Miscellaneous databases
PROi | PR:P0AEJ0 |
Family and domain databases
Gene3Di | 1.20.1250.20, 1 hit |
InterProi | View protein in InterPro IPR004638, EmrB-like IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
TIGRFAMsi | TIGR00711, efflux_EmrB, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EMRB_ECOLI | |
Accessioni | P0AEJ0Primary (citable) accession number: P0AEJ0 Secondary accession number(s): P27304, P77725 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 20, 2005 |
Last sequence update: | December 20, 2005 | |
Last modified: | February 23, 2022 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families