UniProtKB - P0AEI6 (NUDJ_ECOLI)
Protein
Phosphatase NudJ
Gene
nudJ
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF3-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate.2 Publications
Catalytic activityi
Cofactori
Mg2+1 Publication
Kineticsi
- KM=0.64 mM for GDP1 Publication
- Vmax=12 µmol/min/mg enzyme with GDP as substrate1 Publication
pH dependencei
Optimum pH is about 8.5.1 Publication
GO - Molecular functioni
- 4-amino-5-hydroxymethyl-2-methylpyrimidine diphosphatase activity Source: RHEA
- nucleoside-diphosphatase activity Source: EcoCyc
- nucleoside-triphosphatase activity Source: EcoCyc
- pyrophosphatase activity Source: EcoCyc
- thiamine-diphosphatase activity Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Ligand | Magnesium |
Enzyme and pathway databases
BioCyci | EcoCyc:G6580-MONOMER MetaCyc:G6580-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:nudJ Synonyms:ymfB Ordered Locus Names:b1134, JW1120 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
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PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000057069 | 1 – 153 | Phosphatase NudJAdd BLAST | 153 |
Proteomic databases
jPOSTi | P0AEI6 |
PaxDbi | P0AEI6 |
PRIDEi | P0AEI6 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4263128, 44 interactors 850061, 1 interactor |
DIPi | DIP-35908N |
IntActi | P0AEI6, 5 interactors |
STRINGi | 511145.b1134 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 131 | Nudix hydrolasePROSITE-ProRule annotationAdd BLAST | 129 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 36 – 57 | Nudix boxAdd BLAST | 22 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1051, Bacteria |
HOGENOMi | CLU_037162_6_1_6 |
InParanoidi | P0AEI6 |
PhylomeDBi | P0AEI6 |
Family and domain databases
CDDi | cd03675, Nudix_Hydrolase_2, 1 hit |
InterProi | View protein in InterPro IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom IPR033713, NudJ |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEI6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFKPHVTVAC VVHAEGKFLV VEETINGKAL WNQPAGHLEA DETLVEAAAR
60 70 80 90 100
ELWEETGISA QPQHFIRMHQ WIAPDKTPFL RFLFAIELEQ ICPTQPHDSD
110 120 130 140 150
IDCCRWVSAE EILQASNLRS PLVAESIRCY QSGQRYPLEM IGDFNWPFTK
GVI
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74218.1 AP009048 Genomic DNA Translation: BAA35956.1 |
PIRi | C64858 |
RefSeqi | NP_415652.1, NC_000913.3 WP_000476093.1, NZ_STEB01000016.1 |
Genome annotation databases
EnsemblBacteriai | AAC74218; AAC74218; b1134 BAA35956; BAA35956; BAA35956 |
GeneIDi | 58461252 945689 |
KEGGi | ecj:JW1120 eco:b1134 |
PATRICi | fig|1411691.4.peg.1132 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74218.1 AP009048 Genomic DNA Translation: BAA35956.1 |
PIRi | C64858 |
RefSeqi | NP_415652.1, NC_000913.3 WP_000476093.1, NZ_STEB01000016.1 |
3D structure databases
SMRi | P0AEI6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263128, 44 interactors 850061, 1 interactor |
DIPi | DIP-35908N |
IntActi | P0AEI6, 5 interactors |
STRINGi | 511145.b1134 |
Proteomic databases
jPOSTi | P0AEI6 |
PaxDbi | P0AEI6 |
PRIDEi | P0AEI6 |
Genome annotation databases
EnsemblBacteriai | AAC74218; AAC74218; b1134 BAA35956; BAA35956; BAA35956 |
GeneIDi | 58461252 945689 |
KEGGi | ecj:JW1120 eco:b1134 |
PATRICi | fig|1411691.4.peg.1132 |
Organism-specific databases
EchoBASEi | EB3220 |
Phylogenomic databases
eggNOGi | COG1051, Bacteria |
HOGENOMi | CLU_037162_6_1_6 |
InParanoidi | P0AEI6 |
PhylomeDBi | P0AEI6 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6580-MONOMER MetaCyc:G6580-MONOMER |
Miscellaneous databases
PROi | PR:P0AEI6 |
Family and domain databases
CDDi | cd03675, Nudix_Hydrolase_2, 1 hit |
InterProi | View protein in InterPro IPR020476, Nudix_hydrolase IPR015797, NUDIX_hydrolase-like_dom_sf IPR020084, NUDIX_hydrolase_CS IPR000086, NUDIX_hydrolase_dom IPR033713, NudJ |
Pfami | View protein in Pfam PF00293, NUDIX, 1 hit |
PRINTSi | PR00502, NUDIXFAMILY |
SUPFAMi | SSF55811, SSF55811, 1 hit |
PROSITEi | View protein in PROSITE PS51462, NUDIX, 1 hit PS00893, NUDIX_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NUDJ_ECOLI | |
Accessioni | P0AEI6Primary (citable) accession number: P0AEI6 Secondary accession number(s): P75965 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 107 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families