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Protein

Phosphatase NudJ

Gene

nudJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of 4-amino-2-methyl-5-hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2-methyl-5-hydroxymethylpyrimidine phosphate (HMP-P), and hydrolysis of thiamine pyrophosphate (TPP) to thiamine monophosphate (TMP). Can hydrolyze other substrates such as MeO-HMP-PP, CF3-HMP-PP and MeO-TPP. Is also a non-specific nucleoside tri- and diphosphatase that releases inorganic orthophosphate.2 Publications

Cofactori

Mg2+1 Publication

Kineticsi

  1. KM=0.64 mM for GDP1 Publication
  1. Vmax=12 µmol/min/mg enzyme with GDP as substrate1 Publication

pH dependencei

Optimum pH is about 8.5.1 Publication

GO - Molecular functioni

  • nucleoside-diphosphatase activity Source: EcoCyc
  • nucleoside-triphosphatase activity Source: EcoCyc
  • thiamine-pyrophosphatase activity Source: EcoCyc

Keywordsi

Molecular functionHydrolase
LigandMagnesium

Enzyme and pathway databases

BioCyciEcoCyc:G6580-MONOMER
MetaCyc:G6580-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatase NudJ (EC:3.6.1.-)
Gene namesi
Name:nudJ
Synonyms:ymfB
Ordered Locus Names:b1134, JW1120
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13446 nudJ

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570691 – 153Phosphatase NudJAdd BLAST153

Proteomic databases

PaxDbiP0AEI6
PRIDEiP0AEI6

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi4263128, 44 interactors
DIPiDIP-35908N
IntActiP0AEI6, 5 interactors
STRINGi316385.ECDH10B_1206

Structurei

3D structure databases

ProteinModelPortaliP0AEI6
SMRiP0AEI6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 131Nudix hydrolasePROSITE-ProRule annotationAdd BLAST129

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi36 – 57Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. NudJ subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105KB7 Bacteria
COG0494 LUCA
HOGENOMiHOG000256353
InParanoidiP0AEI6
KOiK12152
OMAiQPAGHLD
PhylomeDBiP0AEI6

Family and domain databases

CDDicd03675 Nudix_Hydrolase_2, 1 hit
InterProiView protein in InterPro
IPR020476 Nudix_hydrolase
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
IPR033713 NudJ
PfamiView protein in Pfam
PF00293 NUDIX, 1 hit
PRINTSiPR00502 NUDIXFAMILY
SUPFAMiSSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequencei

Sequence statusi: Complete.

P0AEI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKPHVTVAC VVHAEGKFLV VEETINGKAL WNQPAGHLEA DETLVEAAAR
60 70 80 90 100
ELWEETGISA QPQHFIRMHQ WIAPDKTPFL RFLFAIELEQ ICPTQPHDSD
110 120 130 140 150
IDCCRWVSAE EILQASNLRS PLVAESIRCY QSGQRYPLEM IGDFNWPFTK

GVI
Length:153
Mass (Da):17,433
Last modified:December 6, 2005 - v1
Checksum:iBDB7423E97D6735A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74218.1
AP009048 Genomic DNA Translation: BAA35956.1
PIRiC64858
RefSeqiNP_415652.1, NC_000913.3
WP_000476093.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74218; AAC74218; b1134
BAA35956; BAA35956; BAA35956
GeneIDi945689
KEGGiecj:JW1120
eco:b1134
PATRICifig|1411691.4.peg.1132

Similar proteinsi

Entry informationi

Entry nameiNUDJ_ECOLI
AccessioniPrimary (citable) accession number: P0AEI6
Secondary accession number(s): P75965
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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