UniProtKB - P0AEG6 (DSBC_ECOLI)
Protein
Thiol:disulfide interchange protein DsbC
Gene
dsbC
Organism
Escherichia coli (strain K12)
Status
Functioni
Acts as a disulfide isomerase, interacting with incorrectly folded proteins to correct non-native disulfide bonds. DsbG and DsbC are part of a periplasmic reducing system that controls the level of cysteine sulfenylation, and provides reducing equivalents to rescue oxidatively damaged secreted proteins. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbC is reoxidized by DsbD.1 Publication
GO - Molecular functioni
- protein disulfide isomerase activity Source: EcoCyc
- protein disulfide oxidoreductase activity Source: UniProtKB
GO - Biological processi
- chaperone-mediated protein folding Source: EcoCyc
- response to copper ion Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:DSBC-MONOMER MetaCyc:DSBC-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Thiol:disulfide interchange protein DsbC |
Gene namesi | Name:dsbC Synonyms:xprA Ordered Locus Names:b2893, JW2861 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Other locations
- Periplasm 1 Publication
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
- periplasmic space Source: EcoliWiki
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 118 | C → T or A: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 121 | C → A: Partial loss of activity. 1 Publication | 1 | |
Mutagenesisi | 121 | C → V: Loss of activity. 1 Publication | 1 | |
Mutagenesisi | 161 | C → S: Destabilization of protein. 1 Publication | 1 | |
Mutagenesisi | 183 | C → L: Destabilization of protein. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 20 | 1 PublicationAdd BLAST | 20 | |
ChainiPRO_0000034273 | 21 – 236 | Thiol:disulfide interchange protein DsbCAdd BLAST | 216 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 118 ↔ 121 | Redox-activePROSITE-ProRule annotation1 Publication | ||
Disulfide bondi | 161 ↔ 183 | 1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P0AEG6 |
PaxDbi | P0AEG6 |
PRIDEi | P0AEG6 |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 4261304, 500 interactors |
DIPi | DIP-35818N |
IntActi | P0AEG6, 9 interactors |
STRINGi | 511145.b2893 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEG6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEG6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 231 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 196 |
Sequence similaritiesi
Keywords - Domaini
Redox-active center, SignalPhylogenomic databases
eggNOGi | COG1651, Bacteria |
HOGENOMi | CLU_083593_0_0_6 |
InParanoidi | P0AEG6 |
PhylomeDBi | P0AEG6 |
Family and domain databases
CDDi | cd03020, DsbA_DsbC_DsbG, 1 hit |
Gene3Di | 3.10.450.70, 1 hit 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR033954, DiS-bond_Isoase_DsbC/G IPR018950, DiS-bond_isomerase_DsbC/G_N IPR009094, DiS-bond_isomerase_DsbC/G_N_sf IPR012336, Thioredoxin-like_fold IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10411, DsbC_N, 1 hit PF13098, Thioredoxin_2, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit SSF54423, SSF54423, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEG6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKGFMLFTL LAAFSGFAQA DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV
60 70 80 90 100
LTNSGVLYIT DDGKHIIQGP MYDVSGTAPV NVTNKMLLKQ LNALEKEMIV
110 120 130 140 150
YKAPQEKHVI TVFTDITCGY CHKLHEQMAD YNALGITVRY LAFPRQGLDS
160 170 180 190 200
DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YALGVQLGVS
210 220 230
GTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 219 | Missing in AAA62788 (PubMed:1987126).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54884 Genomic DNA Translation: AAA62788.1 U28375 Genomic DNA Translation: AAA83074.1 U00096 Genomic DNA Translation: AAC75931.1 AP009048 Genomic DNA Translation: BAE76958.1 |
PIRi | E65073 |
RefSeqi | NP_417369.1, NC_000913.3 WP_000715214.1, NZ_STEB01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC75931; AAC75931; b2893 BAE76958; BAE76958; BAE76958 |
GeneIDi | 947363 |
KEGGi | ecj:JW2861 eco:b2893 |
PATRICi | fig|1411691.4.peg.3840 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M54884 Genomic DNA Translation: AAA62788.1 U28375 Genomic DNA Translation: AAA83074.1 U00096 Genomic DNA Translation: AAC75931.1 AP009048 Genomic DNA Translation: BAE76958.1 |
PIRi | E65073 |
RefSeqi | NP_417369.1, NC_000913.3 WP_000715214.1, NZ_STEB01000001.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EEJ | X-ray | 1.90 | A/B | 21-236 | [»] | |
1G0T | X-ray | 2.60 | A/B | 21-236 | [»] | |
1JZD | X-ray | 2.30 | A/B | 20-236 | [»] | |
1JZO | X-ray | 1.92 | A/B | 21-236 | [»] | |
1TJD | X-ray | 2.50 | A | 21-236 | [»] | |
2IYJ | X-ray | 2.00 | A/B | 19-91 | [»] | |
SMRi | P0AEG6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261304, 500 interactors |
DIPi | DIP-35818N |
IntActi | P0AEG6, 9 interactors |
STRINGi | 511145.b2893 |
Chemistry databases
DrugBanki | DB03814, 2-(N-morpholino)ethanesulfonic acid |
Proteomic databases
jPOSTi | P0AEG6 |
PaxDbi | P0AEG6 |
PRIDEi | P0AEG6 |
Genome annotation databases
EnsemblBacteriai | AAC75931; AAC75931; b2893 BAE76958; BAE76958; BAE76958 |
GeneIDi | 947363 |
KEGGi | ecj:JW2861 eco:b2893 |
PATRICi | fig|1411691.4.peg.3840 |
Organism-specific databases
EchoBASEi | EB1063 |
Phylogenomic databases
eggNOGi | COG1651, Bacteria |
HOGENOMi | CLU_083593_0_0_6 |
InParanoidi | P0AEG6 |
PhylomeDBi | P0AEG6 |
Enzyme and pathway databases
BioCyci | EcoCyc:DSBC-MONOMER MetaCyc:DSBC-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AEG6 |
PROi | PR:P0AEG6 |
Family and domain databases
CDDi | cd03020, DsbA_DsbC_DsbG, 1 hit |
Gene3Di | 3.10.450.70, 1 hit 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR033954, DiS-bond_Isoase_DsbC/G IPR018950, DiS-bond_isomerase_DsbC/G_N IPR009094, DiS-bond_isomerase_DsbC/G_N_sf IPR012336, Thioredoxin-like_fold IPR036249, Thioredoxin-like_sf IPR017937, Thioredoxin_CS IPR013766, Thioredoxin_domain |
Pfami | View protein in Pfam PF10411, DsbC_N, 1 hit PF13098, Thioredoxin_2, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit SSF54423, SSF54423, 1 hit |
PROSITEi | View protein in PROSITE PS00194, THIOREDOXIN_1, 1 hit PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DSBC_ECOLI | |
Accessioni | P0AEG6Primary (citable) accession number: P0AEG6 Secondary accession number(s): P21892, Q2M9U8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | April 7, 2021 | |
This is version 121 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families