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Protein

Thiol:disulfide interchange protein DsbA

Gene

dsbA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cellular response to antibiotic Source: EcoliWiki

Enzyme and pathway databases

BioCyciEcoCyc:DISULFOXRED-MONOMER
MetaCyc:DISULFOXRED-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol:disulfide interchange protein DsbA
Gene namesi
Name:dsbA
Synonyms:dsf, ppfA
Ordered Locus Names:b3860, JW3832
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11297 dsbA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Induction of the PhoP/PhoQ two-component regulatory system, suppressed by 50 µM CuSO4.1 Publication

Chemistry databases

ChEMBLiCHEMBL3559645
DrugBankiDB08689 UBIQUINONE-1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 193 PublicationsAdd BLAST19
ChainiPRO_000003425220 – 208Thiol:disulfide interchange protein DsbAAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 52Redox-activePROSITE-ProRule annotation1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP0AEG4
PaxDbiP0AEG4
PRIDEiP0AEG4

2D gel databases

SWISS-2DPAGEiP0AEG4

Interactioni

Binary interactionsi

Protein-protein interaction databases

BioGridi4261200, 185 interactors
DIPiDIP-35886N
IntActiP0AEG4, 20 interactors
MINTiP0AEG4
STRINGi316385.ECDH10B_4049

Chemistry databases

BindingDBiP0AEG4

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP0AEG4
SMRiP0AEG4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0AEG4

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 150ThioredoxinPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the thioredoxin family. DsbA subfamily.Curated

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiENOG4108Z33 Bacteria
COG0526 LUCA
HOGENOMiHOG000265316
InParanoidiP0AEG4
KOiK03673
PhylomeDBiP0AEG4

Family and domain databases

CDDicd03019 DsbA_DsbA, 1 hit
InterProiView protein in InterPro
IPR001853 DSBA-like_thioredoxin_dom
IPR023205 DsbA/DsbL
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF01323 DSBA, 1 hit
PIRSFiPIRSF001488 Tdi_protein, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0AEG4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP
60 70 80 90 100
HCYQFEEVLH ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA
110 120 130 140 150
LGVEDKVTVP LFEGVQKTQT IRSASDIRDV FINAGIKGEE YDAAWNSFVV
160 170 180 190 200
KSLVAQQEKA AADVQLRGVP AMFVNGKYQL NPQGMDTSNM DVFVQQYADT

VKYLSEKK
Length:208
Mass (Da):23,105
Last modified:December 6, 2005 - v1
Checksum:i10527E876FCAEE55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80762 Genomic DNA Translation: CAA56736.1
M77746 Genomic DNA Translation: AAA23715.1
X63186 Genomic DNA Translation: CAA44868.1
L19201 Genomic DNA Translation: AAB02995.1
U00096 Genomic DNA Translation: AAC76858.1
AP009048 Genomic DNA Translation: BAE77448.1
U35817 Genomic DNA Translation: AAC43519.1
U35818 Genomic DNA Translation: AAC43520.1
U35819 Genomic DNA Translation: AAC43521.1
U35820 Genomic DNA Translation: AAC43522.1
U35821 Genomic DNA Translation: AAC43523.1
U35822 Genomic DNA Translation: AAC43524.1
U35823 Genomic DNA Translation: AAC43525.1
U35824 Genomic DNA Translation: AAC43526.1
U35825 Genomic DNA Translation: AAC43527.1
U36828 Genomic DNA Translation: AAC43528.1
U36829 Genomic DNA Translation: AAC43529.1
U36830 Genomic DNA Translation: AAC43530.1
U36831 Genomic DNA Translation: AAC43531.1
U36832 Genomic DNA Translation: AAC43532.1
U36833 Genomic DNA Translation: AAC43533.1
U36834 Genomic DNA Translation: AAC43534.1
U36835 Genomic DNA Translation: AAC43535.1
U35662 Genomic DNA Translation: AAA82614.1
PIRiA39292
RefSeqiNP_418297.1, NC_000913.3
WP_000725337.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76858; AAC76858; b3860
BAE77448; BAE77448; BAE77448
GeneIDi948353
KEGGiecj:JW3832
eco:b3860
PATRICifig|1411691.4.peg.2855

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80762 Genomic DNA Translation: CAA56736.1
M77746 Genomic DNA Translation: AAA23715.1
X63186 Genomic DNA Translation: CAA44868.1
L19201 Genomic DNA Translation: AAB02995.1
U00096 Genomic DNA Translation: AAC76858.1
AP009048 Genomic DNA Translation: BAE77448.1
U35817 Genomic DNA Translation: AAC43519.1
U35818 Genomic DNA Translation: AAC43520.1
U35819 Genomic DNA Translation: AAC43521.1
U35820 Genomic DNA Translation: AAC43522.1
U35821 Genomic DNA Translation: AAC43523.1
U35822 Genomic DNA Translation: AAC43524.1
U35823 Genomic DNA Translation: AAC43525.1
U35824 Genomic DNA Translation: AAC43526.1
U35825 Genomic DNA Translation: AAC43527.1
U36828 Genomic DNA Translation: AAC43528.1
U36829 Genomic DNA Translation: AAC43529.1
U36830 Genomic DNA Translation: AAC43530.1
U36831 Genomic DNA Translation: AAC43531.1
U36832 Genomic DNA Translation: AAC43532.1
U36833 Genomic DNA Translation: AAC43533.1
U36834 Genomic DNA Translation: AAC43534.1
U36835 Genomic DNA Translation: AAC43535.1
U35662 Genomic DNA Translation: AAA82614.1
PIRiA39292
RefSeqiNP_418297.1, NC_000913.3
WP_000725337.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A23NMR-A20-208[»]
1A24NMR-A20-208[»]
1A2JX-ray2.00A20-208[»]
1A2LX-ray2.70A/B20-208[»]
1A2MX-ray2.70A/B20-208[»]
1AC1X-ray2.00A/B20-208[»]
1ACVX-ray1.90A/B20-208[»]
1BQ7X-ray2.80A/B/C/D/E/F20-208[»]
1DSBX-ray2.00A/B20-208[»]
1FVJX-ray2.06A/B20-208[»]
1FVKX-ray1.70A/B20-208[»]
1TI1X-ray2.60A20-208[»]
1U3AX-ray2.00A/B/D/E20-208[»]
1UN2X-ray2.40A20-208[»]
2B3SX-ray1.96A/B20-208[»]
2B6MX-ray2.65A/B20-208[»]
2HI7X-ray3.70A20-208[»]
2LEGNMR-A20-208[»]
2NDONMR-A20-208[»]
2ZUPX-ray3.70A20-208[»]
3E9JX-ray3.70B/E20-208[»]
4TKYX-ray2.50A/B/C/D20-208[»]
4ZIJX-ray1.78A/B20-207[»]
6BQXX-ray1.99A/B20-208[»]
6BR4X-ray1.99A/B20-208[»]
ProteinModelPortaliP0AEG4
SMRiP0AEG4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261200, 185 interactors
DIPiDIP-35886N
IntActiP0AEG4, 20 interactors
MINTiP0AEG4
STRINGi316385.ECDH10B_4049

Chemistry databases

BindingDBiP0AEG4
ChEMBLiCHEMBL3559645
DrugBankiDB08689 UBIQUINONE-1

2D gel databases

SWISS-2DPAGEiP0AEG4

Proteomic databases

EPDiP0AEG4
PaxDbiP0AEG4
PRIDEiP0AEG4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76858; AAC76858; b3860
BAE77448; BAE77448; BAE77448
GeneIDi948353
KEGGiecj:JW3832
eco:b3860
PATRICifig|1411691.4.peg.2855

Organism-specific databases

EchoBASEiEB1274
EcoGeneiEG11297 dsbA

Phylogenomic databases

eggNOGiENOG4108Z33 Bacteria
COG0526 LUCA
HOGENOMiHOG000265316
InParanoidiP0AEG4
KOiK03673
PhylomeDBiP0AEG4

Enzyme and pathway databases

BioCyciEcoCyc:DISULFOXRED-MONOMER
MetaCyc:DISULFOXRED-MONOMER

Miscellaneous databases

EvolutionaryTraceiP0AEG4
PROiPR:P0AEG4

Family and domain databases

CDDicd03019 DsbA_DsbA, 1 hit
InterProiView protein in InterPro
IPR001853 DSBA-like_thioredoxin_dom
IPR023205 DsbA/DsbL
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF01323 DSBA, 1 hit
PIRSFiPIRSF001488 Tdi_protein, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS00194 THIOREDOXIN_1, 1 hit
PS51352 THIOREDOXIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDSBA_ECOLI
AccessioniPrimary (citable) accession number: P0AEG4
Secondary accession number(s): P24991
, Q2M8F8, Q46951, Q46952
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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