UniProtKB - P0AEE8 (DMA_ECOLI)
Protein
DNA adenine methylase
Gene
dam
Organism
Escherichia coli (strain K12)
Status
Functioni
Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 10 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 14 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 | |
Binding sitei | 54 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 181 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- S-adenosyl-L-methionine binding Source: EcoCyc
- sequence-specific DNA binding Source: EcoCyc
- site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoCyc
GO - Biological processi
- bacterial-type DNA replication initiation Source: EcoCyc
- DNA-dependent DNA replication Source: EcoliWiki
- DNA methylation on adenine Source: EcoCyc
- mismatch repair Source: EcoliWiki
- response to UV Source: EcoCyc
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | DNA replication |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10204-MONOMER MetaCyc:EG10204-MONOMER |
BRENDAi | 2.1.1.72, 2026 |
Protein family/group databases
REBASEi | 13376, M.EcoW3110DamP 2396, M.EcoKDam |
Names & Taxonomyi
Protein namesi | Recommended name: DNA adenine methylase (EC:2.1.1.72)Alternative name(s): DNA adenine methyltransferase Deoxyadenosyl-methyltransferase M.EcoDam |
Gene namesi | Name:dam Ordered Locus Names:b3387, JW3350 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087991 | 1 – 278 | DNA adenine methylaseAdd BLAST | 278 |
Proteomic databases
jPOSTi | P0AEE8 |
PaxDbi | P0AEE8 |
PRIDEi | P0AEE8 |
Interactioni
Binary interactionsi
P0AEE8
With | #Exp. | IntAct |
---|---|---|
glpD [P13035] | 3 | EBI-548491,EBI-548509 |
Protein-protein interaction databases
BioGRIDi | 4261860, 125 interactors |
DIPi | DIP-47948N |
IntActi | P0AEE8, 24 interactors |
STRINGi | 511145.b3387 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEE8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEE8 |
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Phylogenomic databases
eggNOGi | COG0338, Bacteria |
HOGENOMi | CLU_063430_0_1_6 |
InParanoidi | P0AEE8 |
PhylomeDBi | P0AEE8 |
Family and domain databases
Gene3Di | 1.10.1020.10, 1 hit |
InterProi | View protein in InterPro IPR023095, Ade_MeTrfase_dom_2 IPR002052, DNA_methylase_N6_adenine_CS IPR012263, M_m6A_EcoRV IPR012327, MeTrfase_D12 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR30481, PTHR30481, 1 hit |
Pfami | View protein in Pfam PF02086, MethyltransfD12, 1 hit |
PIRSFi | PIRSF000398, M_m6A_EcoRV, 1 hit |
PRINTSi | PR00505, D12N6MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00571, dam, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEE8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY
60 70 80 90 100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK
110 120 130 140 150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH
160 170 180 190 200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS
210 220 230 240 250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS
260 270
ISSNGGTRKK VDELLALYKP GVVSPAKK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01600 Genomic DNA Translation: AAA23664.1 V00272 Genomic DNA Translation: CAA23530.1 Z19601 Genomic DNA Translation: CAA79668.1 U18997 Genomic DNA Translation: AAA58184.1 U00096 Genomic DNA Translation: AAC76412.1 AP009048 Genomic DNA Translation: BAE77904.1 X15162 Genomic DNA Translation: CAA33254.1 |
PIRi | A00555, XYECDA |
RefSeqi | NP_417846.1, NC_000913.3 WP_000742143.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76412; AAC76412; b3387 BAE77904; BAE77904; BAE77904 |
GeneIDi | 57729349 947893 |
KEGGi | ecj:JW3350 eco:b3387 |
PATRICi | fig|1411691.4.peg.3343 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | J01600 Genomic DNA Translation: AAA23664.1 V00272 Genomic DNA Translation: CAA23530.1 Z19601 Genomic DNA Translation: CAA79668.1 U18997 Genomic DNA Translation: AAA58184.1 U00096 Genomic DNA Translation: AAC76412.1 AP009048 Genomic DNA Translation: BAE77904.1 X15162 Genomic DNA Translation: CAA33254.1 |
PIRi | A00555, XYECDA |
RefSeqi | NP_417846.1, NC_000913.3 WP_000742143.1, NZ_STEB01000004.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2G1P | X-ray | 1.89 | A/B | 1-278 | [»] | |
2ORE | X-ray | 2.99 | D/E/F | 1-278 | [»] | |
4GBE | X-ray | 2.66 | D/E/F | 1-278 | [»] | |
4GOL | X-ray | 2.57 | D/E/F | 1-278 | [»] | |
4GOM | X-ray | 2.45 | D/E/F | 1-278 | [»] | |
4GON | X-ray | 2.72 | D/E/F | 1-278 | [»] | |
4GOO | X-ray | 2.70 | D/E/F | 1-278 | [»] | |
4RTJ | X-ray | 1.99 | A | 1-278 | [»] | |
4RTK | X-ray | 1.96 | A | 1-278 | [»] | |
4RTL | X-ray | 2.19 | A | 1-278 | [»] | |
4RTM | X-ray | 2.50 | A | 1-278 | [»] | |
4RTN | X-ray | 2.59 | A | 1-278 | [»] | |
4RTO | X-ray | 2.69 | A | 1-278 | [»] | |
4RTP | X-ray | 2.39 | A | 1-278 | [»] | |
4RTQ | X-ray | 2.00 | A | 1-278 | [»] | |
4RTR | X-ray | 2.39 | A | 1-278 | [»] | |
4RTS | X-ray | 2.49 | A | 1-278 | [»] | |
SMRi | P0AEE8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261860, 125 interactors |
DIPi | DIP-47948N |
IntActi | P0AEE8, 24 interactors |
STRINGi | 511145.b3387 |
Chemistry databases
ChEMBLi | CHEMBL1075075 |
Protein family/group databases
REBASEi | 13376, M.EcoW3110DamP 2396, M.EcoKDam |
Proteomic databases
jPOSTi | P0AEE8 |
PaxDbi | P0AEE8 |
PRIDEi | P0AEE8 |
Genome annotation databases
EnsemblBacteriai | AAC76412; AAC76412; b3387 BAE77904; BAE77904; BAE77904 |
GeneIDi | 57729349 947893 |
KEGGi | ecj:JW3350 eco:b3387 |
PATRICi | fig|1411691.4.peg.3343 |
Organism-specific databases
EchoBASEi | EB0200 |
Phylogenomic databases
eggNOGi | COG0338, Bacteria |
HOGENOMi | CLU_063430_0_1_6 |
InParanoidi | P0AEE8 |
PhylomeDBi | P0AEE8 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10204-MONOMER MetaCyc:EG10204-MONOMER |
BRENDAi | 2.1.1.72, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AEE8 |
PROi | PR:P0AEE8 |
Family and domain databases
Gene3Di | 1.10.1020.10, 1 hit |
InterProi | View protein in InterPro IPR023095, Ade_MeTrfase_dom_2 IPR002052, DNA_methylase_N6_adenine_CS IPR012263, M_m6A_EcoRV IPR012327, MeTrfase_D12 IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR30481, PTHR30481, 1 hit |
Pfami | View protein in Pfam PF02086, MethyltransfD12, 1 hit |
PIRSFi | PIRSF000398, M_m6A_EcoRV, 1 hit |
PRINTSi | PR00505, D12N6MTFRASE |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00571, dam, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DMA_ECOLI | |
Accessioni | P0AEE8Primary (citable) accession number: P0AEE8 Secondary accession number(s): P00475, Q2M752 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | April 7, 2021 | |
This is version 126 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families