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UniProtKB - P0AEE8 (DMA_ECOLI)

Entry version 129 (25 May 2022)
Sequence version 1 (21 Jul 1986)
Previous versions | rss
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Protein

DNA adenine methylase

Gene

dam

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

An alpha subtype methylase that recognizes the double-stranded sequence 5'-GATC-3' and methylates A-2 on both strands (Probable) (PubMed:12654995).

Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in methyl-directed mismatch repair, initiation of chromosome replication and gene expression.

1 Publication1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei10S-adenosyl-L-methionineBy similarity1
Binding sitei14S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei54S-adenosyl-L-methionineBy similarity1
Binding sitei181S-adenosyl-L-methionineBy similarity1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding, Methyltransferase, Transferase
Biological processDNA replication
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		EcoCyc:EG10204-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.72, 2026

Protein family/group databases

Restriction enzymes and methylases database

More...REBASEi		13376, M.EcoW3110DamP
2396, M.EcoKDam
441639, M.EcoBL21FDamP

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA adenine methylase1 Publication (EC:2.1.1.72)
Alternative name(s):
DNA adenine methyltransferase
Deoxyadenosyl-methyltransferase
Orphan methyltransferase M.EcoKDam1 Publication
Short name:
M.EcoKDam1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:dam1 Publication
Ordered Locus Names:b3387, JW3350
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi134P → S: About 63% methylase activity, binds S-adenosyl-methionine (SAM) normally. 1 Publication1
Mutagenesisi136G → A: About 33% methylase activity, binds SAM normally. 1 Publication1
Mutagenesisi137R → L: No methylase activity, binds SAM normally. 1 Publication1
Mutagenesisi139K → S or T: 75% methylase activity, binds SAM normally. 1 Publication1
Mutagenesisi181D → G, N or S: Protein stable, no methylase activity, no binding to S-adenosyl-methionine. 1 Publication1
Mutagenesisi183P → E or G: Low amounts of protein, no methyase activity, no binding to S-adenosyl-methionine. 1 Publication1
Mutagenesisi183P → R: Protein stable, no methylase activity, no binding to S-adenosyl-methionine. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1075075

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000879911 – 278DNA adenine methylaseAdd BLAST278

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		P0AEE8

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		P0AEE8

PRoteomics IDEntifications database

More...PRIDEi		P0AEE8

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		4261860, 125 interactors

Database of interacting proteins

More...DIPi		DIP-47948N

Protein interaction database and analysis system

More...IntActi		P0AEE8, 24 interactors

STRING: functional protein association networks

More...STRINGi		511145.b3387

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1278
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

AlphaFold Protein Structure Database

More...AlphaFoldDBi		P0AEE8

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		P0AEE8

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		P0AEE8

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		COG0338, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_063430_0_1_6

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		P0AEE8

Identification of Orthologs from Complete Genome Data

More...OMAi		MNRHGFN

Database for complete collections of gene phylogenies

More...PhylomeDBi		P0AEE8

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.10.1020.10, 1 hit
3.40.50.150, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR023095, Ade_MeTrfase_dom_2
IPR002052, DNA_methylase_N6_adenine_CS
IPR012263, M_m6A_EcoRV
IPR012327, MeTrfase_D12
IPR029063, SAM-dependent_MTases

The PANTHER Classification System

More...PANTHERi		PTHR30481, PTHR30481, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF02086, MethyltransfD12, 1 hit

PIRSF; a whole-protein classification database

More...PIRSFi		PIRSF000398, M_m6A_EcoRV, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00505, D12N6MTFRASE

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53335, SSF53335, 1 hit

TIGRFAMs; a protein family database

More...TIGRFAMsi		TIGR00571, dam, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00092, N6_MTASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P0AEE8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY 
        60         70         80         90        100
ILADINSDLI SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK 
       110        120        130        140        150
SQDPFRRAVL FLYLNRYGYN GLCRYNLRGE FNVPFGRYKK PYFPEAELYH 
       160        170        180        190        200
FAEKAQNAFF YCESYADSMA RADDASVVYC DPPYAPLSAT ANFTAYHTNS 
       210        220        230        240        250
FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA KLHVVKVRRS 
       260        270 
ISSNGGTRKK VDELLALYKP GVVSPAKK
Length:278
Mass (Da):32,100
Last modified:July 21, 1986 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iB2FA5D1FDC863CB9
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
J01600 Genomic DNA Translation: AAA23664.1
V00272 Genomic DNA Translation: CAA23530.1
Z19601 Genomic DNA Translation: CAA79668.1
U18997 Genomic DNA Translation: AAA58184.1
U00096 Genomic DNA Translation: AAC76412.1
AP009048 Genomic DNA Translation: BAE77904.1
X15162 Genomic DNA Translation: CAA33254.1

Protein sequence database of the Protein Information Resource

More...PIRi		A00555, XYECDA

NCBI Reference Sequences

More...RefSeqi		NP_417846.1, NC_000913.3
WP_000742143.1, NZ_STEB01000004.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAC76412; AAC76412; b3387
BAE77904; BAE77904; BAE77904

Database of genes from NCBI RefSeq genomes

More...GeneIDi		66672733
947893

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		ecj:JW3350
eco:b3387

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|1411691.4.peg.3343

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01600 Genomic DNA Translation: AAA23664.1
V00272 Genomic DNA Translation: CAA23530.1
Z19601 Genomic DNA Translation: CAA79668.1
U18997 Genomic DNA Translation: AAA58184.1
U00096 Genomic DNA Translation: AAC76412.1
AP009048 Genomic DNA Translation: BAE77904.1
X15162 Genomic DNA Translation: CAA33254.1
PIRiA00555, XYECDA
RefSeqiNP_417846.1, NC_000913.3
WP_000742143.1, NZ_STEB01000004.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2G1PX-ray1.89A/B1-278[»]
2OREX-ray2.99D/E/F1-278[»]
4GBEX-ray2.66D/E/F1-278[»]
4GOLX-ray2.57D/E/F1-278[»]
4GOMX-ray2.45D/E/F1-278[»]
4GONX-ray2.72D/E/F1-278[»]
4GOOX-ray2.70D/E/F1-278[»]
4RTJX-ray1.99A1-278[»]
4RTKX-ray1.96A1-278[»]
4RTLX-ray2.19A1-278[»]
4RTMX-ray2.50A1-278[»]
4RTNX-ray2.59A1-278[»]
4RTOX-ray2.69A1-278[»]
4RTPX-ray2.39A1-278[»]
4RTQX-ray2.00A1-278[»]
4RTRX-ray2.39A1-278[»]
4RTSX-ray2.49A1-278[»]
AlphaFoldDBiP0AEE8
SMRiP0AEE8
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi4261860, 125 interactors
DIPiDIP-47948N
IntActiP0AEE8, 24 interactors
STRINGi511145.b3387

Chemistry databases

ChEMBLiCHEMBL1075075

Protein family/group databases

REBASEi13376, M.EcoW3110DamP
2396, M.EcoKDam
441639, M.EcoBL21FDamP

Proteomic databases

jPOSTiP0AEE8
PaxDbiP0AEE8
PRIDEiP0AEE8

Genome annotation databases

EnsemblBacteriaiAAC76412; AAC76412; b3387
BAE77904; BAE77904; BAE77904
GeneIDi66672733
947893
KEGGiecj:JW3350
eco:b3387
PATRICifig|1411691.4.peg.3343

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...EchoBASEi		EB0200

Phylogenomic databases

eggNOGiCOG0338, Bacteria
HOGENOMiCLU_063430_0_1_6
InParanoidiP0AEE8
OMAiMNRHGFN
PhylomeDBiP0AEE8

Enzyme and pathway databases

BioCyciEcoCyc:EG10204-MONOMER
BRENDAi2.1.1.72, 2026

Miscellaneous databases

EvolutionaryTraceiP0AEE8

Protein Ontology

More...PROi		PR:P0AEE8

Family and domain databases

Gene3Di1.10.1020.10, 1 hit
3.40.50.150, 1 hit
InterProiView protein in InterPro
IPR023095, Ade_MeTrfase_dom_2
IPR002052, DNA_methylase_N6_adenine_CS
IPR012263, M_m6A_EcoRV
IPR012327, MeTrfase_D12
IPR029063, SAM-dependent_MTases
PANTHERiPTHR30481, PTHR30481, 1 hit
PfamiView protein in Pfam
PF02086, MethyltransfD12, 1 hit
PIRSFiPIRSF000398, M_m6A_EcoRV, 1 hit
PRINTSiPR00505, D12N6MTFRASE
SUPFAMiSSF53335, SSF53335, 1 hit
TIGRFAMsiTIGR00571, dam, 1 hit
PROSITEiView protein in PROSITE
PS00092, N6_MTASE, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDMA_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P0AEE8
Secondary accession number(s): P00475, Q2M752
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 25, 2022
This is version 129 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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