UniProtKB - P0AEE5 (DGAL_ECOLI)
Protein
D-galactose-binding periplasmic protein
Gene
mglB
Organism
Escherichia coli (strain K12)
Status
Functioni
This protein is involved in the active transport of galactose and glucose. It plays a role in the chemotaxis towards the two sugars by interacting with the trg chemoreceptor.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 97 | Interacts with membrane-bound trg signal transducer | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 157 – 165 | First part of site | 9 | |
Calcium bindingi | 227 – 228 | Second part of site | 2 |
GO - Molecular functioni
- carbohydrate binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- chemotaxis Source: EcoCyc
- galactose transmembrane transport Source: EcoCyc
- methylgalactoside transport Source: EcoCyc
Keywordsi
Biological process | Chemotaxis, Sugar transport, Transport |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:MGLB-MONOMER MetaCyc:MGLB-MONOMER |
Protein family/group databases
TCDBi | 3.A.1.2.3, the atp-binding cassette (abc) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: D-galactose-binding periplasmic proteinShort name: GBP Alternative name(s): D-galactose/ D-glucose-binding protein Short name: GGBP |
Gene namesi | Name:mglB Ordered Locus Names:b2150, JW2137 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
GO - Cellular componenti
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
PeriplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 97 | G → D: Improductive interaction with trg. | 1 |
Chemistry databases
DrugBanki | DB02379, Beta-D-Glucose |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | 2 PublicationsAdd BLAST | 23 | |
ChainiPRO_0000031722 | 24 – 332 | D-galactose-binding periplasmic proteinAdd BLAST | 309 |
Proteomic databases
jPOSTi | P0AEE5 |
PaxDbi | P0AEE5 |
PRIDEi | P0AEE5 |
2D gel databases
SWISS-2DPAGEi | P0AEE5 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259172, 16 interactors 853285, 1 interactor |
ComplexPortali | CPX-4341, Beta-methyl-D-galactoside/galactose ABC transporter complex |
DIPi | DIP-35953N |
IntActi | P0AEE5, 8 interactors |
STRINGi | 511145.b2150 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AEE5 |
SMRi | P0AEE5 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEE5 |
Family & Domainsi
Domaini
The calcium-binding site is structurally similar to that of EF-hand proteins, but is in two parts, with the last calcium ligand provided by Glu-228.
Sequence similaritiesi
Belongs to the bacterial solute-binding protein 2 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1879, Bacteria |
HOGENOMi | CLU_037628_3_1_6 |
InParanoidi | P0AEE5 |
PhylomeDBi | P0AEE5 |
Family and domain databases
CDDi | cd01539, PBP1_GGBP, 1 hit |
InterProi | View protein in InterPro IPR044085, MglB-like_PBP1 IPR028082, Peripla_BP_I IPR025997, SBP_2_dom |
Pfami | View protein in Pfam PF13407, Peripla_BP_4, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEE5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNKKVLTLSA VMASMLFGAA AHAADTRIGV TIYKYDDNFM SVVRKAIEQD
60 70 80 90 100
AKAAPDVQLL MNDSQNDQSK QNDQIDVLLA KGVKALAINL VDPAAAGTVI
110 120 130 140 150
EKARGQNVPV VFFNKEPSRK ALDSYDKAYY VGTDSKESGI IQGDLIAKHW
160 170 180 190 200
AANQGWDLNK DGQIQFVLLK GEPGHPDAEA RTTYVIKELN DKGIKTEQLQ
210 220 230 240 250
LDTAMWDTAQ AKDKMDAWLS GPNANKIEVV IANNDAMAMG AVEALKAHNK
260 270 280 290 300
SSIPVFGVDA LPEALALVKS GALAGTVLND ANNQAKATFD LAKNLADGKG
310 320 330
AADGTNWKID NKVVRVPYVG VDKDNLAEFS KK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59444 Genomic DNA Translation: AAA24169.1 U00007 Genomic DNA Translation: AAA60523.1 X05646 Genomic DNA Translation: CAA29132.1 U00096 Genomic DNA Translation: AAC75211.1 AP009048 Genomic DNA Translation: BAE76627.1 K00419 Genomic DNA Translation: AAA24172.1 |
PIRi | A37277, JGECG |
RefSeqi | NP_416655.1, NC_000913.3 WP_001036964.1, NZ_STEB01000002.1 |
Genome annotation databases
EnsemblBacteriai | AAC75211; AAC75211; b2150 BAE76627; BAE76627; BAE76627 |
GeneIDi | 49584029 949041 |
KEGGi | ecj:JW2137 eco:b2150 |
PATRICi | fig|1411691.4.peg.91 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M59444 Genomic DNA Translation: AAA24169.1 U00007 Genomic DNA Translation: AAA60523.1 X05646 Genomic DNA Translation: CAA29132.1 U00096 Genomic DNA Translation: AAC75211.1 AP009048 Genomic DNA Translation: BAE76627.1 K00419 Genomic DNA Translation: AAA24172.1 |
PIRi | A37277, JGECG |
RefSeqi | NP_416655.1, NC_000913.3 WP_001036964.1, NZ_STEB01000002.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GLG | X-ray | 2.00 | A | 24-332 | [»] | |
2FVY | X-ray | 0.92 | A | 24-332 | [»] | |
2FW0 | X-ray | 1.55 | A | 24-332 | [»] | |
2GBP | X-ray | 1.90 | A | 24-332 | [»] | |
2HPH | X-ray | 1.33 | A | 24-332 | [»] | |
2IPL | X-ray | 1.20 | A | 24-332 | [»] | |
2IPM | X-ray | 1.12 | A | 24-332 | [»] | |
2IPN | X-ray | 1.15 | A | 24-332 | [»] | |
2QW1 | X-ray | 1.70 | A | 24-332 | [»] | |
BMRBi | P0AEE5 | |||||
SMRi | P0AEE5 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259172, 16 interactors 853285, 1 interactor |
ComplexPortali | CPX-4341, Beta-methyl-D-galactoside/galactose ABC transporter complex |
DIPi | DIP-35953N |
IntActi | P0AEE5, 8 interactors |
STRINGi | 511145.b2150 |
Chemistry databases
DrugBanki | DB02379, Beta-D-Glucose |
Protein family/group databases
TCDBi | 3.A.1.2.3, the atp-binding cassette (abc) superfamily |
2D gel databases
SWISS-2DPAGEi | P0AEE5 |
Proteomic databases
jPOSTi | P0AEE5 |
PaxDbi | P0AEE5 |
PRIDEi | P0AEE5 |
Genome annotation databases
EnsemblBacteriai | AAC75211; AAC75211; b2150 BAE76627; BAE76627; BAE76627 |
GeneIDi | 49584029 949041 |
KEGGi | ecj:JW2137 eco:b2150 |
PATRICi | fig|1411691.4.peg.91 |
Organism-specific databases
EchoBASEi | EB0588 |
Phylogenomic databases
eggNOGi | COG1879, Bacteria |
HOGENOMi | CLU_037628_3_1_6 |
InParanoidi | P0AEE5 |
PhylomeDBi | P0AEE5 |
Enzyme and pathway databases
BioCyci | EcoCyc:MGLB-MONOMER MetaCyc:MGLB-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P0AEE5 |
PROi | PR:P0AEE5 |
Family and domain databases
CDDi | cd01539, PBP1_GGBP, 1 hit |
InterProi | View protein in InterPro IPR044085, MglB-like_PBP1 IPR028082, Peripla_BP_I IPR025997, SBP_2_dom |
Pfami | View protein in Pfam PF13407, Peripla_BP_4, 1 hit |
SUPFAMi | SSF53822, SSF53822, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGAL_ECOLI | |
Accessioni | P0AEE5Primary (citable) accession number: P0AEE5 Secondary accession number(s): P02927, P17775, Q2MAS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | August 13, 1987 | |
Last modified: | December 2, 2020 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families