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Protein

DNA-cytosine methyltransferase

Gene

dcm

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei177PROSITE-ProRule annotation1

GO - Molecular functioni

  • DNA (cytosine-5-)-methyltransferase activity Source: EcoCyc

GO - Biological processi

  • C-5 methylation of cytosine Source: EcoCyc
  • DNA restriction-modification system Source: UniProtKB-KW

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processRestriction system
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10211-MONOMER
MetaCyc:EG10211-MONOMER

Protein family/group databases

REBASEi2397 M.EcoKDcm

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-cytosine methyltransferase (EC:2.1.1.37)
Alternative name(s):
M.EcoDcm
Gene namesi
Name:dcm
Synonyms:mec
Ordered Locus Names:b1961, JW1944
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10211 dcm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000879131 – 472DNA-cytosine methyltransferaseAdd BLAST472

Proteomic databases

PaxDbiP0AED9
PRIDEiP0AED9

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
aceFP069593EBI-548525,EBI-542707

Protein-protein interaction databases

BioGridi4260865, 162 interactors
DIPiDIP-47858N
IntActiP0AED9, 21 interactors
STRINGi316385.ECDH10B_2103

Structurei

3D structure databases

ProteinModelPortaliP0AED9
SMRiP0AED9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 457SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST371

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105DVF Bacteria
COG0270 LUCA
HOGENOMiHOG000225506
InParanoidiP0AED9
KOiK00558
OMAiAGYRKGF
PhylomeDBiP0AED9

Family and domain databases

InterProiView protein in InterPro
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00094 C5_MTASE_1, 1 hit
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P0AED9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQENISVTDS YSTGNAAQAM LEKLLQIYDV KTLVAQLNGV GENHWSAAIL
60 70 80 90 100
KRALANDSAW HRLSEKEFAH LQTLLPKPPA HHPHYAFRFI DLFAGIGGIR
110 120 130 140 150
RGFESIGGQC VFTSEWNKHA VRTYKANHYC DPATHHFNED IRDITLSHKE
160 170 180 190 200
GVSDEAAAEH IRQHIPEHDV LLAGFPCQPF SLAGVSKKNS LGRAHGFACD
210 220 230 240 250
TQGTLFFDVV RIIDARRPAM FVLENVKNLK SHDQGKTFRI IMQTLDELGY
260 270 280 290 300
DVADAEDNGP DDPKIIDGKH FLPQHRERIV LVGFRRDLNL KADFTLRDIS
310 320 330 340 350
ECFPAQRVTL AQLLDPMVEA KYILTPVLWK YLYRYAKKHQ ARGNGFGYGM
360 370 380 390 400
VYPNNPQSVT RTLSARYYKD GAEILIDRGW DMATGEKDFD DPLNQQHRPR
410 420 430 440 450
RLTPRECARL MGFEAPGEAK FRIPVSDTQA YRQFGNSVVV PVFAAVAKLL
460 470
EPKIKQAVAL RQQEAQHGRR SR
Length:472
Mass (Da):53,465
Last modified:December 6, 2005 - v1
Checksum:i3635BFC001CF94B4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X13330 Genomic DNA Translation: CAA31705.1
M32307 Unassigned DNA Translation: AAA03723.1
U00096 Genomic DNA Translation: AAC75027.1
AP009048 Genomic DNA Translation: BAA15788.1
PIRiA37754 JS0263
RefSeqiNP_416470.1, NC_000913.3
WP_001157239.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75027; AAC75027; b1961
BAA15788; BAA15788; BAA15788
GeneIDi946479
KEGGiecj:JW1944
eco:b1961
PATRICifig|1411691.4.peg.291

Similar proteinsi

Entry informationi

Entry nameiDCM_ECOLI
AccessioniPrimary (citable) accession number: P0AED9
Secondary accession number(s): P11876
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 6, 2005
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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