UniProtKB - P0AED7 (DAPE_ECOLI)
Protein
Succinyl-diaminopimelate desuccinylase
Gene
dapE
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.1 Publication
Miscellaneous
DapE/msgB is a multicopy suppressor of a temperature-sensitive allele of the heat shock gene grpE.
Catalytic activityi
- EC:3.5.1.18
Cofactori
Protein has several cofactor binding sites:- Zn2+1 PublicationNote: Binds 1 Zn2+ ion per subunit.1 Publication
- Co2+1 PublicationNote: Binds 1 Co2+ ion per subunit.1 Publication
Kineticsi
- KM=0.41 mM for L,L-SDAP (in the presence of Zn2+ at pH 7.0)1 Publication
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route).Proteins known to be involved in the 3 steps of the subpathway in this organism are:
- 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD), 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)
- Acetylornithine/succinyldiaminopimelate aminotransferase (argD), Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
- Succinyl-diaminopimelate desuccinylase (dapE), Succinyl-diaminopimelate desuccinylase (dapE)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes LL-2,6-diaminopimelate from (S)-tetrahydrodipicolinate (succinylase route), the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 66 | Cobalt or zinc 1By similarity | 1 | |
Active sitei | 68 | By similarity | 1 | |
Metal bindingi | 99 | Cobalt or zinc 1By similarity | 1 | |
Metal bindingi | 99 | Cobalt or zinc 2By similarity | 1 | |
Active sitei | 133 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 134 | Cobalt or zinc 2By similarity | 1 | |
Metal bindingi | 162 | Cobalt or zinc 1By similarity | 1 | |
Metal bindingi | 348 | Cobalt or zinc 2By similarity | 1 |
GO - Molecular functioni
- cobalt ion binding Source: EcoCyc
- identical protein binding Source: EcoCyc
- succinyl-diaminopimelate desuccinylase activity Source: EcoCyc
- zinc ion binding Source: EcoCyc
GO - Biological processi
- diaminopimelate biosynthetic process Source: UniProtKB-UniRule
- FtsZ-dependent cytokinesis Source: GO_Central
- lysine biosynthetic process via diaminopimelate Source: EcoCyc
Keywordsi
Molecular function | Hydrolase |
Biological process | Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis |
Ligand | Cobalt, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | EcoCyc:MONOMER0-1981 MetaCyc:MONOMER0-1981 |
SABIO-RKi | P0AED7 |
UniPathwayi | UPA00034;UER00021 |
Protein family/group databases
MEROPSi | M20.010 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinyl-diaminopimelate desuccinylase (EC:3.5.1.18)Short name: SDAP desuccinylase Alternative name(s): N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase |
Gene namesi | Name:dapE Synonyms:msgB Ordered Locus Names:b2472, JW2456 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185261 | 1 – 375 | Succinyl-diaminopimelate desuccinylaseAdd BLAST | 375 |
Proteomic databases
jPOSTi | P0AED7 |
PaxDbi | P0AED7 |
PRIDEi | P0AED7 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261716, 4 interactors 852610, 3 interactors |
IntActi | P0AED7, 6 interactors |
STRINGi | 511145.b2472 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0624, Bacteria |
HOGENOMi | CLU_021802_4_0_6 |
InParanoidi | P0AED7 |
PhylomeDBi | P0AED7 |
Family and domain databases
HAMAPi | MF_01690, DapE, 1 hit |
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR036264, Bact_exopeptidase_dim_dom IPR005941, DapE_proteobac IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
TIGRFAMsi | TIGR01246, dapE_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS00758, ARGE_DAPE_CPG2_1, 1 hit PS00759, ARGE_DAPE_CPG2_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AED7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSCPVIELTQ QLIRRPSLSP DDAGCQALLI ERLQAIGFTV ERMDFADTQN
60 70 80 90 100
FWAWRGQGET LAFAGHTDVV PPGDADRWIN PPFEPTIRDG MLFGRGAADM
110 120 130 140 150
KGSLAAMVVA AERFVAQHPN HTGRLAFLIT SDEEASAHNG TVKVVEALMA
160 170 180 190 200
RNERLDYCLV GEPSSIEVVG DVVKNGRRGS LTCNLTIHGV QGHVAYPHLA
210 220 230 240 250
DNPVHRAAPF LNELVAIEWD QGNEFFPATS MQIANIQAGT GSNNVIPGEL
260 270 280 290 300
FVQFNFRFST ELTDEMIKAQ VLALLEKHQL RYTVDWWLSG QPFLTARGKL
310 320 330 340 350
VDAVVNAVEH YNEIKPQLLT TGGTSDGRFI ARMGAQVVEL GPVNATIHKI
360 370
NECVNAADLQ LLARMYQRIM EQLVA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S41760 Genomic DNA Translation: AAB22798.1 X57403 Genomic DNA Translation: CAA40665.1 U00096 Genomic DNA Translation: AAC75525.1 AP009048 Genomic DNA Translation: BAA16346.1 |
PIRi | A42959 |
RefSeqi | NP_416967.1, NC_000913.3 WP_001277801.1, NZ_STEB01000051.1 |
Genome annotation databases
EnsemblBacteriai | AAC75525; AAC75525; b2472 BAA16346; BAA16346; BAA16346 |
GeneIDi | 58388524 948313 |
KEGGi | ecj:JW2456 eco:b2472 |
PATRICi | fig|1411691.4.peg.4268 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | S41760 Genomic DNA Translation: AAB22798.1 X57403 Genomic DNA Translation: CAA40665.1 U00096 Genomic DNA Translation: AAC75525.1 AP009048 Genomic DNA Translation: BAA16346.1 |
PIRi | A42959 |
RefSeqi | NP_416967.1, NC_000913.3 WP_001277801.1, NZ_STEB01000051.1 |
3D structure databases
SMRi | P0AED7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4261716, 4 interactors 852610, 3 interactors |
IntActi | P0AED7, 6 interactors |
STRINGi | 511145.b2472 |
Protein family/group databases
MEROPSi | M20.010 |
Proteomic databases
jPOSTi | P0AED7 |
PaxDbi | P0AED7 |
PRIDEi | P0AED7 |
Genome annotation databases
EnsemblBacteriai | AAC75525; AAC75525; b2472 BAA16346; BAA16346; BAA16346 |
GeneIDi | 58388524 948313 |
KEGGi | ecj:JW2456 eco:b2472 |
PATRICi | fig|1411691.4.peg.4268 |
Organism-specific databases
EchoBASEi | EB0204 |
Phylogenomic databases
eggNOGi | COG0624, Bacteria |
HOGENOMi | CLU_021802_4_0_6 |
InParanoidi | P0AED7 |
PhylomeDBi | P0AED7 |
Enzyme and pathway databases
UniPathwayi | UPA00034;UER00021 |
BioCyci | EcoCyc:MONOMER0-1981 MetaCyc:MONOMER0-1981 |
SABIO-RKi | P0AED7 |
Miscellaneous databases
PROi | PR:P0AED7 |
Family and domain databases
HAMAPi | MF_01690, DapE, 1 hit |
InterProi | View protein in InterPro IPR001261, ArgE/DapE_CS IPR036264, Bact_exopeptidase_dim_dom IPR005941, DapE_proteobac IPR002933, Peptidase_M20 IPR011650, Peptidase_M20_dimer |
Pfami | View protein in Pfam PF07687, M20_dimer, 1 hit PF01546, Peptidase_M20, 1 hit |
SUPFAMi | SSF55031, SSF55031, 1 hit |
TIGRFAMsi | TIGR01246, dapE_proteo, 1 hit |
PROSITEi | View protein in PROSITE PS00758, ARGE_DAPE_CPG2_1, 1 hit PS00759, ARGE_DAPE_CPG2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DAPE_ECOLI | |
Accessioni | P0AED7Primary (citable) accession number: P0AED7 Secondary accession number(s): P24176 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | February 10, 2021 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families