UniProtKB - P0AEC8 (DCUS_ECOLI)
Protein
Sensor histidine kinase DcuS
Gene
dcuS
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system DcuR/DcuS. Involved in the C4-dicarboxylate-stimulated regulation of the genes encoding the anaerobic fumarate respiratory system (frdABCD; nuoAN; dcuB; dcuC; sdhCDAB; etc.). Weakly regulates the aerobic C4-dicarboxylate transporter dctA. Activates DcuR by phosphorylation.
Miscellaneous
The region encompassing approximately residues 42 to 181 has been shown to be periplasmic, however exactly which residues are periplasmic is not clear.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
Activity regulationi
Autophosphorylation is stimulated by the presence of C4-dicarboxylates such as fumarate, succinate, malate, and tartrate.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 107 | C4-dicarboxylate | 1 | |
Binding sitei | 110 | C4-dicarboxylate | 1 | |
Binding sitei | 121 | C4-dicarboxylate; via amide nitrogen and carbonyl oxygen | 1 | |
Binding sitei | 147 | C4-dicarboxylate | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: IntAct
- phosphorelay sensor kinase activity Source: EcoCyc
- protein histidine kinase activity Source: EcoliWiki
GO - Biological processi
- phosphorelay signal transduction system Source: EcoliWiki
- protein autophosphorylation Source: EcoCyc
- protein phosphorylation Source: EcoliWiki
- regulation of transcription, DNA-templated Source: EcoliWiki
- signal transduction by protein phosphorylation Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:DCUS-MONOMER MetaCyc:DCUS-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dcuS Synonyms:yjdH Ordered Locus Names:b4125, JW4086 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 20 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 21 – 41 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 42 – 181 | PeriplasmicSequence analysisAdd BLAST | 140 | |
Transmembranei | 182 – 202 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 203 – 543 | CytoplasmicSequence analysisAdd BLAST | 341 |
GO - Cellular componenti
- cytosol Source: EcoCyc
- integral component of membrane Source: EcoliWiki
- integral component of plasma membrane Source: EcoCyc
- outer membrane-bounded periplasmic space Source: EcoCyc
- plasma membrane Source: EcoliWiki
- protein histidine kinase complex Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 107 | R → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication | 1 | |
Mutagenesisi | 110 | H → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication | 1 | |
Mutagenesisi | 147 | R → A: Abolishes the stimulation by fumarate to the same extent as complete deletion of the dcuS gene. 1 Publication | 1 | |
Mutagenesisi | 248 | N → A, D or G: Causes constitutive active state of the kinase, leading to constitutive expression of a target gene, without addition of C4-dicarboxylates. 1 Publication | 1 | |
Mutagenesisi | 304 | N → D: Causes constitutive active state of the kinase, leading to constitutive expression of a target gene, without addition of C4-dicarboxylates. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074750 | 1 – 543 | Sensor histidine kinase DcuSAdd BLAST | 543 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 349 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 |
Post-translational modificationi
Autophosphorylated. The phosphoryl group is rapidly transferred to DcuR.
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AEC8 |
PaxDbi | P0AEC8 |
PRIDEi | P0AEC8 |
Interactioni
Subunit structurei
Homodimer.
2 PublicationsBinary interactionsi
P0AEC8
With | #Exp. | IntAct |
---|---|---|
itself | 8 | EBI-1134683,EBI-1134683 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4261981, 13 interactors |
DIPi | DIP-9414N |
IntActi | P0AEC8, 3 interactors |
STRINGi | 511145.b4125 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P0AEC8 |
SMRi | P0AEC8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEC8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 212 – 323 | PASPROSITE-ProRule annotationAdd BLAST | 112 | |
Domaini | 346 – 538 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 193 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 140 – 142 | C4-dicarboxylate binding | 3 |
Domaini
The periplasmic domain is involved in C4-dicarboxylate binding and sensing. The structural disorder in the cytoplasmic PAS domain has an important role in signal transduction to the kinase domain and may be the decisive structural feature that characterizes the activated kinase.3 Publications
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG3290, Bacteria |
HOGENOMi | CLU_020211_11_2_6 |
InParanoidi | P0AEC8 |
PhylomeDBi | P0AEC8 |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR033463, sCache_3 IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR016120, Sig_transdc_His_kin_SpoOB IPR039506, SPOB_a |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00989, PAS, 1 hit PF17203, sCache_3_2, 1 hit PF14689, SPOB_a, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit SSF55890, SSF55890, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEC8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRHSLPYRML RKRPMKLSTT VILMVSAVLF SVLLVVHLIY FSQISDMTRD
60 70 80 90 100
GLANKALAVA RTLADSPEIR QGLQKKPQES GIQAIAEAVR KRNDLLFIVV
110 120 130 140 150
TDMQSLRYSH PEAQRIGQPF KGDDILKALN GEENVAINRG FLAQALRVFT
160 170 180 190 200
PIYDENHKQI GVVAIGLELS RVTQQINDSR WSIIWSVLFG MLVGLIGTCI
210 220 230 240 250
LVKVLKKILF GLEPYEISTL FEQRQAMLQS IKEGVVAVDD RGEVTLINDA
260 270 280 290 300
AQELLNYRKS QDDEKLSTLS HSWSQVVDVS EVLRDGTPRR DEEITIKDRL
310 320 330 340 350
LLINTVPVRS NGVIIGAIST FRDKTEVRKL MQRLDGLVNY ADALRERSHE
360 370 380 390 400
FMNKLHVILG LLHLKSYKQL EDYILKTANN YQEEIGSLLG KIKSPVIAGF
410 420 430 440 450
LISKINRATD LGHTLILNSE SQLPDSGSED QVATLITTLG NLIENALEAL
460 470 480 490 500
GPEPGGEISV TLHYRHGWLH CEVNDDGPGI APDKIDHIFD KGVSTKGSER
510 520 530 540
GVGLALVKQQ VENLGGSIAV ESEPGIFTQF FVQIPWDGER SNR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97025.1 U00096 Genomic DNA Translation: AAC77086.1 AP009048 Genomic DNA Translation: BAE78127.1 |
PIRi | D65222 |
RefSeqi | NP_418549.1, NC_000913.3 WP_001216477.1, NZ_SSZK01000018.1 |
Genome annotation databases
EnsemblBacteriai | AAC77086; AAC77086; b4125 BAE78127; BAE78127; BAE78127 |
GeneIDi | 48723402 948639 |
KEGGi | ecj:JW4086 eco:b4125 |
PATRICi | fig|1411691.4.peg.2575 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U14003 Genomic DNA Translation: AAA97025.1 U00096 Genomic DNA Translation: AAC77086.1 AP009048 Genomic DNA Translation: BAE78127.1 |
PIRi | D65222 |
RefSeqi | NP_418549.1, NC_000913.3 WP_001216477.1, NZ_SSZK01000018.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OJG | NMR | - | A | 45-180 | [»] | |
2W0N | NMR | - | A | 211-325 | [»] | |
3BY8 | X-ray | 1.45 | A | 42-181 | [»] | |
BMRBi | P0AEC8 | |||||
SMRi | P0AEC8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261981, 13 interactors |
DIPi | DIP-9414N |
IntActi | P0AEC8, 3 interactors |
STRINGi | 511145.b4125 |
Proteomic databases
jPOSTi | P0AEC8 |
PaxDbi | P0AEC8 |
PRIDEi | P0AEC8 |
Genome annotation databases
EnsemblBacteriai | AAC77086; AAC77086; b4125 BAE78127; BAE78127; BAE78127 |
GeneIDi | 48723402 948639 |
KEGGi | ecj:JW4086 eco:b4125 |
PATRICi | fig|1411691.4.peg.2575 |
Organism-specific databases
EchoBASEi | EB2358 |
Phylogenomic databases
eggNOGi | COG3290, Bacteria |
HOGENOMi | CLU_020211_11_2_6 |
InParanoidi | P0AEC8 |
PhylomeDBi | P0AEC8 |
Enzyme and pathway databases
BioCyci | EcoCyc:DCUS-MONOMER MetaCyc:DCUS-MONOMER |
BRENDAi | 2.7.13.3, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AEC8 |
PROi | PR:P0AEC8 |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold IPR033463, sCache_3 IPR029151, Sensor-like_sf IPR004358, Sig_transdc_His_kin-like_C IPR016120, Sig_transdc_His_kin_SpoOB IPR039506, SPOB_a |
Pfami | View protein in Pfam PF02518, HATPase_c, 1 hit PF00989, PAS, 1 hit PF17203, sCache_3_2, 1 hit PF14689, SPOB_a, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00387, HATPase_c, 1 hit SM00091, PAS, 1 hit |
SUPFAMi | SSF103190, SSF103190, 1 hit SSF55785, SSF55785, 1 hit SSF55874, SSF55874, 1 hit SSF55890, SSF55890, 1 hit |
PROSITEi | View protein in PROSITE PS50109, HIS_KIN, 1 hit PS50112, PAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCUS_ECOLI | |
Accessioni | P0AEC8Primary (citable) accession number: P0AEC8 Secondary accession number(s): P39272, P76795, Q2M6H9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references