UniProtKB - P0AEC5 (BARA_ECOLI)
Protein
Signal transduction histidine-protein kinase BarA
Gene
barA
Organism
Escherichia coli (strain K12)
Status
Functioni
Member of the two-component regulatory system UvrY/BarA involved in the regulation of carbon metabolism via the CsrA/CsrB regulatory system. Phosphorylates UvrY, probably via a four-step phosphorelay.
Catalytic activityi
- ATP + protein L-histidine = ADP + protein N-phospho-L-histidine. EC:2.7.13.3
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- histidine phosphotransfer kinase activity Source: EcoliWiki
- phosphoprotein phosphatase activity Source: EcoCyc
- phosphorelay sensor kinase activity Source: EcoCyc
- protein histidine kinase activity Source: EcoCyc
GO - Biological processi
- protein autophosphorylation Source: EcoliWiki
- protein phosphorylation Source: EcoliWiki
- response to acetate Source: EcoCyc
- response to formic acid Source: EcoCyc
- response to hydrogen peroxide Source: EcoCyc
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Transcription, Transcription regulation, Two-component regulatory system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:BARA-MONOMER MetaCyc:BARA-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:barA Ordered Locus Names:b2786, JW2757 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 9 | CytoplasmicSequence analysis | 9 | |
Transmembranei | 10 – 31 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 32 – 176 | PeriplasmicSequence analysisAdd BLAST | 145 | |
Transmembranei | 177 – 196 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 197 – 918 | CytoplasmicSequence analysisAdd BLAST | 722 |
GO - Cellular componenti
- integral component of plasma membrane Source: EcoCyc
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074697 | 1 – 918 | Signal transduction histidine-protein kinase BarAAdd BLAST | 918 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 302 | Phosphohistidine; by autocatalysisPROSITE-ProRule annotation | 1 | |
Modified residuei | 718 | 4-aspartylphosphatePROSITE-ProRule annotation | 1 | |
Modified residuei | 861 | PhosphohistidinePROSITE-ProRule annotation | 1 |
Post-translational modificationi
Activation requires a sequential transfer of a phosphate group from a His in the primary transmitter domain, to an Asp in the receiver domain and to a His in the secondary transmitter domain.By similarity
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AEC5 |
PaxDbi | P0AEC5 |
PRIDEi | P0AEC5 |
PTM databases
iPTMneti | P0AEC5 |
Interactioni
Binary interactionsi
P0AEC5
With | #Exp. | IntAct |
---|---|---|
clpA [P0ABH9] | 3 | EBI-546740,EBI-546140 |
Protein-protein interaction databases
BioGRIDi | 4262286, 24 interactors 851585, 1 interactor |
DIPi | DIP-47932N |
IntActi | P0AEC5, 4 interactors |
STRINGi | 511145.b2786 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 200 – 252 | HAMPPROSITE-ProRule annotationAdd BLAST | 53 | |
Domaini | 299 – 520 | Histidine kinasePROSITE-ProRule annotationAdd BLAST | 222 | |
Domaini | 669 – 785 | Response regulatoryPROSITE-ProRule annotationAdd BLAST | 117 | |
Domaini | 822 – 918 | HPtPROSITE-ProRule annotationAdd BLAST | 97 |
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2205, Bacteria COG3437, Bacteria COG3850, Bacteria COG4999, Bacteria |
HOGENOMi | CLU_000445_104_1_6 |
InParanoidi | P0AEC5 |
PhylomeDBi | P0AEC5 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit cd00088, HPT, 1 hit cd00156, REC, 1 hit |
Gene3Di | 1.20.120.160, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR019247, Histidine_kinase_BarA_N IPR036641, HPT_dom_sf IPR004358, Sig_transdc_His_kin-like_C IPR008207, Sig_transdc_His_kin_Hpt_dom IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF01627, Hpt, 1 hit PF00072, Response_reg, 1 hit PF09984, sCache_4, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00073, HPT, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF47226, SSF47226, 1 hit SSF47384, SSF47384, 1 hit SSF52172, SSF52172, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit PS50894, HPT, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEC5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTNYSLRARM MILILAPTVL IGLLLSIFFV VHRYNDLQRQ LEDAGASIIE
60 70 80 90 100
PLAVSTEYGM SLQNRESIGQ LISVLHRRHS DIVRAISVYD ENNRLFVTSN
110 120 130 140 150
FHLDPSSMQL GSNVPFPRQL TVTRDGDIMI LRTPIISESY SPDESPSSDA
160 170 180 190 200
KNSQNMLGYI ALELDLKSVR LQQYKEIFIS SVMMLFCIGI ALIFGWRLMR
210 220 230 240 250
DVTGPIRNMV NTVDRIRRGQ LDSRVEGFML GELDMLKNGI NSMAMSLAAY
260 270 280 290 300
HEEMQHNIDQ ATSDLRETLE QMEIQNVELD LAKKRAQEAA RIKSEFLANM
310 320 330 340 350
SHELRTPLNG VIGFTRLTLK TELTPTQRDH LNTIERSANN LLAIINDVLD
360 370 380 390 400
FSKLEAGKLI LESIPFPLRS TLDEVVTLLA HSSHDKGLEL TLNIKSDVPD
410 420 430 440 450
NVIGDPLRLQ QIITNLVGNA IKFTENGNID ILVEKRALSN TKVQIEVQIR
460 470 480 490 500
DTGIGIPERD QSRLFQAFRQ ADASISRRHG GTGLGLVITQ KLVNEMGGDI
510 520 530 540 550
SFHSQPNRGS TFWFHINLDL NPNIIIEGPS TQCLAGKRLA YVEPNSAAAQ
560 570 580 590 600
CTLDILSETP LEVVYSPTFS ALPPAHYDMM LLGIAVTFRE PLTMQHERLA
610 620 630 640 650
KAVSMTDFLM LALPCHAQVN AEKLKQDGIG ACLLKPLTPT RLLPALTEFC
660 670 680 690 700
HHKQNTLLPV TDESKLAMTV MAVDDNPANL KLIGALLEDM VQHVELCDSG
710 720 730 740 750
HQAVERAKQM PFDLILMDIQ MPDMDGIRAC ELIHQLPHQQ QTPVIAVTAH
760 770 780 790 800
AMAGQKEKLL GAGMSDYLAK PIEEERLHNL LLRYKPGSGI SSRVVTPEVN
810 820 830 840 850
EIVVNPNATL DWQLALRQAA GKTDLARDML QMLLDFLPEV RNKVEEQLVG
860 870 880 890 900
ENPEGLVDLI HKLHGSCGYS GVPRMKNLCQ LIEQQLRSGT KEEDLEPELL
910
ELLDEMDNVA REASKILG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10888 Genomic DNA Translation: BAA01710.1 U29580 Genomic DNA Translation: AAA69296.1 U00096 Genomic DNA Translation: AAC75828.1 AP009048 Genomic DNA Translation: BAA16571.2 |
PIRi | S20550 |
RefSeqi | NP_417266.1, NC_000913.3 WP_000186450.1, NZ_SSZK01000003.1 |
Genome annotation databases
EnsemblBacteriai | AAC75828; AAC75828; b2786 BAA16571; BAA16571; BAA16571 |
GeneIDi | 48726382 947255 |
KEGGi | ecj:JW2757 eco:b2786 |
PATRICi | fig|1411691.4.peg.3949 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D10888 Genomic DNA Translation: BAA01710.1 U29580 Genomic DNA Translation: AAA69296.1 U00096 Genomic DNA Translation: AAC75828.1 AP009048 Genomic DNA Translation: BAA16571.2 |
PIRi | S20550 |
RefSeqi | NP_417266.1, NC_000913.3 WP_000186450.1, NZ_SSZK01000003.1 |
3D structure databases
SMRi | P0AEC5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262286, 24 interactors 851585, 1 interactor |
DIPi | DIP-47932N |
IntActi | P0AEC5, 4 interactors |
STRINGi | 511145.b2786 |
PTM databases
iPTMneti | P0AEC5 |
Proteomic databases
jPOSTi | P0AEC5 |
PaxDbi | P0AEC5 |
PRIDEi | P0AEC5 |
Genome annotation databases
EnsemblBacteriai | AAC75828; AAC75828; b2786 BAA16571; BAA16571; BAA16571 |
GeneIDi | 48726382 947255 |
KEGGi | ecj:JW2757 eco:b2786 |
PATRICi | fig|1411691.4.peg.3949 |
Organism-specific databases
EchoBASEi | EB1341 |
Phylogenomic databases
eggNOGi | COG2205, Bacteria COG3437, Bacteria COG3850, Bacteria COG4999, Bacteria |
HOGENOMi | CLU_000445_104_1_6 |
InParanoidi | P0AEC5 |
PhylomeDBi | P0AEC5 |
Enzyme and pathway databases
BioCyci | EcoCyc:BARA-MONOMER MetaCyc:BARA-MONOMER |
Miscellaneous databases
PROi | PR:P0AEC5 |
Family and domain databases
CDDi | cd06225, HAMP, 1 hit cd00082, HisKA, 1 hit cd00088, HPT, 1 hit cd00156, REC, 1 hit |
Gene3Di | 1.20.120.160, 1 hit 3.30.565.10, 1 hit |
InterProi | View protein in InterPro IPR011006, CheY-like_superfamily IPR003660, HAMP_dom IPR003594, HATPase_C IPR036890, HATPase_C_sf IPR005467, His_kinase_dom IPR003661, HisK_dim/P IPR036097, HisK_dim/P_sf IPR019247, Histidine_kinase_BarA_N IPR036641, HPT_dom_sf IPR004358, Sig_transdc_His_kin-like_C IPR008207, Sig_transdc_His_kin_Hpt_dom IPR001789, Sig_transdc_resp-reg_receiver |
Pfami | View protein in Pfam PF00672, HAMP, 1 hit PF02518, HATPase_c, 1 hit PF00512, HisKA, 1 hit PF01627, Hpt, 1 hit PF00072, Response_reg, 1 hit PF09984, sCache_4, 1 hit |
PRINTSi | PR00344, BCTRLSENSOR |
SMARTi | View protein in SMART SM00304, HAMP, 1 hit SM00387, HATPase_c, 1 hit SM00388, HisKA, 1 hit SM00073, HPT, 1 hit SM00448, REC, 1 hit |
SUPFAMi | SSF47226, SSF47226, 1 hit SSF47384, SSF47384, 1 hit SSF52172, SSF52172, 1 hit SSF55874, SSF55874, 1 hit |
PROSITEi | View protein in PROSITE PS50885, HAMP, 1 hit PS50109, HIS_KIN, 1 hit PS50894, HPT, 1 hit PS50110, RESPONSE_REGULATORY, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BARA_ECOLI | |
Accessioni | P0AEC5Primary (citable) accession number: P0AEC5 Secondary accession number(s): P26607, P77032 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | December 2, 2020 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |