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UniProtKB - P0AEB2 (DACA_ECOLI)
Protein
D-alanyl-D-alanine carboxypeptidase DacA
Gene
dacA
Organism
Escherichia coli (strain K12)
Status
Functioni
Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors.
Catalytic activityi
- EC:3.5.2.6
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine. EC:3.4.16.4
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 73 | Acyl-ester intermediate | 1 | |
Active sitei | 76 | Proton acceptor | 1 | |
Active sitei | 139 | 1 | ||
Binding sitei | 242 | Substrate | 1 |
GO - Molecular functioni
- beta-lactamase activity Source: EcoCyc
- carboxypeptidase activity Source: EcoCyc
- penicillin binding Source: EcoCyc
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoliWiki
GO - Biological processi
- cell wall organization Source: UniProtKB-KW
- peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
- peptidoglycan metabolic process Source: EcoCyc
- regulation of cell shape Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Biological process | Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10201-MONOMER |
BRENDAi | 3.4.16.4, 2026 |
UniPathwayi | UPA00219 |
Protein family/group databases
MEROPSi | S11.008 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dacA Synonyms:pfv Ordered Locus Names:b0632, JW0627 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Note: N-terminal lies in the periplasmic space.
Plasma Membrane
- integral component of periplasmic side of plasma membrane Source: EcoCyc
- integral component of plasma membrane Source: EcoliWiki
- plasma membrane Source: EcoCyc
Other locations
- outer membrane-bounded periplasmic space Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 101 – 121 | Missing : Complete loss of enzyme activity. No effect on penicillin binding. 1 PublicationAdd BLAST | 21 | |
Mutagenesisi | 213 | K → R: Complete loss of enzyme activity. No effect on penicillin binding. 1 Publication | 1 | |
Mutagenesisi | 213 | K → X: Complete loss of activity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB04647, BOC-GAMMA-D-GLU-L-LYS(CBZ)-D-BOROALA DB00578, Carbenicillin DB09319, Carindacillin DB00274, Cefmetazole DB01329, Cefoperazone DB01331, Cefoxitin DB09050, Ceftolozane DB01000, Cyclacillin |
DrugCentrali | P0AEB2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 29 | 1 PublicationAdd BLAST | 29 | |
ChainiPRO_0000027231 | 30 – 403 | D-alanyl-D-alanine carboxypeptidase DacAAdd BLAST | 374 |
Proteomic databases
jPOSTi | P0AEB2 |
PaxDbi | P0AEB2 |
PRIDEi | P0AEB2 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260698, 270 interactors |
DIPi | DIP-47947N |
IntActi | P0AEB2, 9 interactors |
STRINGi | 511145.b0632 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P0AEB2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P0AEB2 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase S11 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1686, Bacteria |
HOGENOMi | CLU_027070_8_1_6 |
InParanoidi | P0AEB2 |
PhylomeDBi | P0AEB2 |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P0AEB2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTIFSARIM KRLALTTALC TAFISAAHAD DLNIKTMIPG VPQIDAESYI
60 70 80 90 100
LIDYNSGKVL AEQNADVRRD PASLTKMMTS YVIGQAMKAG KFKETDLVTI
110 120 130 140 150
GNDAWATGNP VFKGSSLMFL KPGMQVPVSQ LIRGINLQSG NDACVAMADF
160 170 180 190 200
AAGSQDAFVG LMNSYVNALG LKNTHFQTVH GLDADGQYSS ARDMALIGQA
210 220 230 240 250
LIRDVPNEYS IYKEKEFTFN GIRQLNRNGL LWDNSLNVDG IKTGHTDKAG
260 270 280 290 300
YNLVASATEG QMRLISAVMG GRTFKGREAE SKKLLTWGFR FFETVNPLKV
310 320 330 340 350
GKEFASEPVW FGDSDRASLG VDKDVYLTIP RGRMKDLKAS YVLNSSELHA
360 370 380 390 400
PLQKNQVVGT INFQLDGKTI EQRPLVVLQE IPEGNFFGKI IDYIKLMFHH
WFG
Sequence cautioni
The sequence AAB40832 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 134 | G → D in mutant dacA11191; inactive but still binds penicillin. Blocked in the release of the bound penicilloyl moiety; the mutant also fails to catalyze the D-alanine carboxypeptidase reaction as the hydrolysis of the acyl-enzyme formed with substrate is also blocked and the acyl-enzyme accumulates. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06479 Genomic DNA Translation: CAA29774.1 M18276 Genomic DNA Translation: AAA24553.1 U82598 Genomic DNA Translation: AAB40832.1 Different initiation. U00096 Genomic DNA Translation: AAC73733.1 AP009048 Genomic DNA Translation: BAA35275.1 L07636 Genomic DNA Translation: AAA66340.1 |
PIRi | A28536, ZPECP5 |
RefSeqi | NP_415165.1, NC_000913.3 WP_001092082.1, NZ_STEB01000031.1 |
Genome annotation databases
EnsemblBacteriai | AAC73733; AAC73733; b0632 BAA35275; BAA35275; BAA35275 |
GeneIDi | 66671094 945222 |
KEGGi | ecj:JW0627 eco:b0632 |
PATRICi | fig|511145.12.peg.662 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X06479 Genomic DNA Translation: CAA29774.1 M18276 Genomic DNA Translation: AAA24553.1 U82598 Genomic DNA Translation: AAB40832.1 Different initiation. U00096 Genomic DNA Translation: AAC73733.1 AP009048 Genomic DNA Translation: BAA35275.1 L07636 Genomic DNA Translation: AAA66340.1 |
PIRi | A28536, ZPECP5 |
RefSeqi | NP_415165.1, NC_000913.3 WP_001092082.1, NZ_STEB01000031.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1HD8 | X-ray | 2.30 | A | 30-386 | [»] | |
1NJ4 | X-ray | 1.90 | A | 30-392 | [»] | |
1NZO | X-ray | 1.85 | A | 30-392 | [»] | |
1NZU | X-ray | 2.00 | A | 30-392 | [»] | |
1SDN | X-ray | 2.50 | A | 30-392 | [»] | |
1Z6F | X-ray | 1.60 | A | 30-392 | [»] | |
3BEB | X-ray | 2.00 | A | 30-386 | [»] | |
3BEC | X-ray | 1.60 | A | 30-386 | [»] | |
3MZD | X-ray | 1.90 | A | 30-386 | [»] | |
3MZE | X-ray | 2.10 | A | 30-386 | [»] | |
3MZF | X-ray | 1.50 | A | 30-386 | [»] | |
5J8X | X-ray | 2.53 | A | 30-392 | [»] | |
6NTZ | X-ray | 2.20 | A | 1-403 | [»] | |
SMRi | P0AEB2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260698, 270 interactors |
DIPi | DIP-47947N |
IntActi | P0AEB2, 9 interactors |
STRINGi | 511145.b0632 |
Chemistry databases
ChEMBLi | CHEMBL2354204 |
DrugBanki | DB01602, Bacampicillin DB04647, BOC-GAMMA-D-GLU-L-LYS(CBZ)-D-BOROALA DB00578, Carbenicillin DB09319, Carindacillin DB00274, Cefmetazole DB01329, Cefoperazone DB01331, Cefoxitin DB09050, Ceftolozane DB01000, Cyclacillin |
DrugCentrali | P0AEB2 |
Protein family/group databases
MEROPSi | S11.008 |
Proteomic databases
jPOSTi | P0AEB2 |
PaxDbi | P0AEB2 |
PRIDEi | P0AEB2 |
Genome annotation databases
EnsemblBacteriai | AAC73733; AAC73733; b0632 BAA35275; BAA35275; BAA35275 |
GeneIDi | 66671094 945222 |
KEGGi | ecj:JW0627 eco:b0632 |
PATRICi | fig|511145.12.peg.662 |
Organism-specific databases
EchoBASEi | EB0197 |
Phylogenomic databases
eggNOGi | COG1686, Bacteria |
HOGENOMi | CLU_027070_8_1_6 |
InParanoidi | P0AEB2 |
PhylomeDBi | P0AEB2 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
BioCyci | EcoCyc:EG10201-MONOMER |
BRENDAi | 3.4.16.4, 2026 |
Miscellaneous databases
EvolutionaryTracei | P0AEB2 |
PROi | PR:P0AEB2 |
Family and domain databases
Gene3Di | 2.60.410.10, 1 hit 3.40.710.10, 1 hit |
InterProi | View protein in InterPro IPR012338, Beta-lactam/transpept-like IPR015956, Peniciliin-bd_prot_C_sf IPR018044, Peptidase_S11 IPR012907, Peptidase_S11_C IPR037167, Peptidase_S11_C_sf IPR001967, Peptidase_S11_N |
Pfami | View protein in Pfam PF07943, PBP5_C, 1 hit PF00768, Peptidase_S11, 1 hit |
PRINTSi | PR00725, DADACBPTASE1 |
SMARTi | View protein in SMART SM00936, PBP5_C, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit SSF69189, SSF69189, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DACA_ECOLI | |
Accessioni | P0AEB2Primary (citable) accession number: P0AEB2 Secondary accession number(s): P04287, P77106 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 1987 |
Last sequence update: | March 20, 1987 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families