UniProtKB - P0AEA8 (CYSG_ECOLI)
Protein
Siroheme synthase
Gene
cysG
Organism
Escherichia coli (strain K12)
Status
Functioni
Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation5 Publications
Catalytic activityi
- 2 S-adenosyl-L-methionine + uroporphyrinogen III = H+ + precorrin-2 + 2 S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.107UniRule annotation
- EC:1.3.1.76UniRule annotation
- EC:4.99.1.4UniRule annotation
: adenosylcobalamin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Siroheme synthase (cobA), Siroheme synthase (cysG)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Pathwayi: adenosylcobalamin biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Siroheme synthase (cobA), Siroheme synthase (cysG)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.
Pathwayi: siroheme biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes precorrin-2 from uroporphyrinogen III.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Siroheme synthase (cysG)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes precorrin-2 from uroporphyrinogen III, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.
Pathwayi: siroheme biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes siroheme from sirohydrochlorin.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Siroheme synthase (cysG)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes siroheme from sirohydrochlorin, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.
Pathwayi: siroheme biosynthesis
This protein is involved in step 1 of the subpathway that synthesizes sirohydrochlorin from precorrin-2.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Siroheme synthase (cobA), Siroheme synthase (cysG)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sirohydrochlorin from precorrin-2, the pathway siroheme biosynthesis and in Porphyrin-containing compound metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 225 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 248 | Proton acceptorUniRule annotation | 1 | |
Active sitei | 270 | Proton donorUniRule annotation | 1 | |
Binding sitei | 306 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 382 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 411 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 22 – 23 | NADUniRule annotation | 2 | |
Nucleotide bindingi | 43 – 44 | NADUniRule annotation | 2 |
GO - Molecular functioni
- NAD binding Source: InterPro
- precorrin-2 dehydrogenase activity Source: UniProtKB-UniRule
- sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
- uroporphyrin-III C-methyltransferase activity Source: EcoCyc
GO - Biological processi
- cobalamin biosynthetic process Source: UniProtKB-UniRule
- methylation Source: UniProtKB-KW
- response to osmotic stress Source: EcoCyc
- siroheme biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Lyase, Methyltransferase, Multifunctional enzyme, Oxidoreductase, Transferase |
Biological process | Cobalamin biosynthesis, Porphyrin biosynthesis |
Ligand | NAD, S-adenosyl-L-methionine |
Enzyme and pathway databases
BioCyci | EcoCyc:SIROHEMESYN-MONOMER MetaCyc:SIROHEMESYN-MONOMER |
UniPathwayi | UPA00148;UER00211 UPA00148;UER00222 UPA00262;UER00211 UPA00262;UER00222 UPA00262;UER00376 |
Names & Taxonomyi
Protein namesi | Recommended name: Siroheme synthaseUniRule annotationIncluding the following 3 domains: Uroporphyrinogen-III C-methyltransferaseUniRule annotation (EC:2.1.1.107UniRule annotation) Short name: Urogen III methylaseUniRule annotation Alternative name(s): SUMTUniRule annotation Uroporphyrinogen III methylaseUniRule annotation Short name: UROMUniRule annotation |
Gene namesi | Name:cysGUniRule annotation Ordered Locus Names:b3368, JW3331 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 21 | G → D: It has methyltransferase and ferrochelatase activities but a greatly reduced dehydrogenase activity. It is able to bind AdoMet. 1 Publication | 1 | |
Mutagenesisi | 224 | G → A: It abolishes methyltransferase activity, but has dehydrogenase and ferrochelatase activities. It is unable to bind AdoMet. 1 Publication | 1 | |
Mutagenesisi | 227 | D → A: It has all activities of CysG. 1 Publication | 1 | |
Mutagenesisi | 248 | D → A: It abolishes methyltransferase activity, but has dehydrogenase and ferrochelatase activities. It is able to bind AdoMet. | 1 | |
Mutagenesisi | 270 | K → I: It has all activities of CysG. It is able to bind AdoMet. 1 Publication | 1 | |
Mutagenesisi | 298 | R → L: It abolishes methyltransferase activity, but has dehydrogenase and ferrochelatase activities. It is unable to bind AdoMet. 1 Publication | 1 | |
Mutagenesisi | 303 | D → A: It has all activities of CysG. It is able to bind AdoMet. 1 Publication | 1 | |
Mutagenesisi | 309 | R → L: It abolishes methyltransferase activity, but has dehydrogenase and ferrochelatase activities. It is able to bind AdoMet. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000150378 | 1 – 457 | Siroheme synthaseAdd BLAST | 457 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 128 | PhosphoserineUniRule annotation | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | P0AEA8 |
PaxDbi | P0AEA8 |
PRIDEi | P0AEA8 |
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4262478, 6 interactors |
IntActi | P0AEA8, 1 interactor |
STRINGi | 511145.b3368 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 4 – 204 | Precorrin-2 dehydrogenase /sirohydrochlorin ferrochelataseBy similarityAdd BLAST | 201 | |
Regioni | 216 – 448 | Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd BLAST | 233 | |
Regioni | 301 – 303 | S-adenosyl-L-methionine bindingUniRule annotation | 3 | |
Regioni | 331 – 332 | S-adenosyl-L-methionine bindingUniRule annotation | 2 |
Sequence similaritiesi
In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0007, Bacteria COG1648, Bacteria |
HOGENOMi | CLU_011276_2_0_6 |
InParanoidi | P0AEA8 |
PhylomeDBi | P0AEA8 |
Family and domain databases
CDDi | cd11642, SUMT, 1 hit |
Gene3Di | 1.10.8.210, 1 hit 3.30.160.110, 1 hit 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
HAMAPi | MF_01646, Siroheme_synth, 1 hit |
InterProi | View protein in InterPro IPR000878, 4pyrrol_Mease IPR035996, 4pyrrol_Methylase_sf IPR014777, 4pyrrole_Mease_sub1 IPR014776, 4pyrrole_Mease_sub2 IPR006366, CobA/CysG_C IPR036291, NAD(P)-bd_dom_sf IPR037115, Sirohaem_synt_dimer_dom_sf IPR012409, Sirohaem_synth IPR043152, Sirohaem_synthase-like_central IPR028281, Sirohaem_synthase_central IPR019478, Sirohaem_synthase_dimer_dom IPR006367, Sirohaem_synthase_N IPR003043, Uropor_MeTrfase_CS |
Pfami | View protein in Pfam PF10414, CysG_dimeriser, 1 hit PF14824, Sirohm_synth_M, 1 hit PF00590, TP_methylase, 1 hit |
PIRSFi | PIRSF036426, Sirohaem_synth, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF53790, SSF53790, 1 hit |
TIGRFAMsi | TIGR01469, cobA_cysG_Cterm, 1 hit TIGR01470, cysG_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS00839, SUMT_1, 1 hit PS00840, SUMT_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P0AEA8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDHLPIFCQL RDRDCLIVGG GDVAERKARL LLDAGARLTV NALAFIPQFT
60 70 80 90 100
AWADAGMLTL VEGPFDESLL DTCWLAIAAT DDDALNQRVS EAAEARRIFC
110 120 130 140 150
NVVDAPKAAS FIMPSIIDRS PLMVAVSSGG TSPVLARLLR EKLESLLPLH
160 170 180 190 200
LGQVAKYAGQ LRGRVKQQFA TMGERRRFWE KLFVNDRLAQ SLANNDQKAI
210 220 230 240 250
TETTEQLINE PLDHRGEVVL VGAGPGDAGL LTLKGLQQIQ QADVVVYDRL
260 270 280 290 300
VSDDIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QKGKRVVRLK
310 320 330 340 350
GGDPFIFGRG GEELETLCNA GIPFSVVPGI TAASGCSAYS GIPLTHRDYA
360 370 380 390 400
QSVRLITGHL KTGGELDWEN LAAEKQTLVF YMGLNQAATI QQKLIEHGMP
410 420 430 440 450
GEMPVAIVEN GTAVTQRVID GTLTQLGELA QQMNSPSLII IGRVVGLRDK
LNWFSNH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | R → P in CAA32419 (PubMed:2543955).Curated | 1 | |
Sequence conflicti | 26 | R → P in AAA58165 (PubMed:2200672).Curated | 1 | |
Sequence conflicti | 90 – 91 | SE → RQ in CAA32419 (PubMed:2543955).Curated | 2 | |
Sequence conflicti | 90 – 91 | SE → RQ in AAA58165 (PubMed:2200672).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14202 Genomic DNA Translation: CAA32419.1 U18997 Genomic DNA Translation: AAA58165.1 U00096 Genomic DNA Translation: AAC76393.1 AP009048 Genomic DNA Translation: BAE77922.1 |
PIRi | C65131 |
RefSeqi | NP_417827.1, NC_000913.3 WP_000349855.1, NZ_STEB01000004.1 |
Genome annotation databases
EnsemblBacteriai | AAC76393; AAC76393; b3368 BAE77922; BAE77922; BAE77922 |
GeneIDi | 947880 |
KEGGi | ecj:JW3331 eco:b3368 |
PATRICi | fig|1411691.4.peg.3361 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X14202 Genomic DNA Translation: CAA32419.1 U18997 Genomic DNA Translation: AAA58165.1 U00096 Genomic DNA Translation: AAC76393.1 AP009048 Genomic DNA Translation: BAE77922.1 |
PIRi | C65131 |
RefSeqi | NP_417827.1, NC_000913.3 WP_000349855.1, NZ_STEB01000004.1 |
3D structure databases
SMRi | P0AEA8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4262478, 6 interactors |
IntActi | P0AEA8, 1 interactor |
STRINGi | 511145.b3368 |
Proteomic databases
jPOSTi | P0AEA8 |
PaxDbi | P0AEA8 |
PRIDEi | P0AEA8 |
Genome annotation databases
EnsemblBacteriai | AAC76393; AAC76393; b3368 BAE77922; BAE77922; BAE77922 |
GeneIDi | 947880 |
KEGGi | ecj:JW3331 eco:b3368 |
PATRICi | fig|1411691.4.peg.3361 |
Organism-specific databases
EchoBASEi | EB0185 |
Phylogenomic databases
eggNOGi | COG0007, Bacteria COG1648, Bacteria |
HOGENOMi | CLU_011276_2_0_6 |
InParanoidi | P0AEA8 |
PhylomeDBi | P0AEA8 |
Enzyme and pathway databases
UniPathwayi | UPA00148;UER00211 UPA00148;UER00222 UPA00262;UER00211 UPA00262;UER00222 UPA00262;UER00376 |
BioCyci | EcoCyc:SIROHEMESYN-MONOMER MetaCyc:SIROHEMESYN-MONOMER |
Miscellaneous databases
PROi | PR:P0AEA8 |
Family and domain databases
CDDi | cd11642, SUMT, 1 hit |
Gene3Di | 1.10.8.210, 1 hit 3.30.160.110, 1 hit 3.30.950.10, 1 hit 3.40.1010.10, 1 hit |
HAMAPi | MF_01646, Siroheme_synth, 1 hit |
InterProi | View protein in InterPro IPR000878, 4pyrrol_Mease IPR035996, 4pyrrol_Methylase_sf IPR014777, 4pyrrole_Mease_sub1 IPR014776, 4pyrrole_Mease_sub2 IPR006366, CobA/CysG_C IPR036291, NAD(P)-bd_dom_sf IPR037115, Sirohaem_synt_dimer_dom_sf IPR012409, Sirohaem_synth IPR043152, Sirohaem_synthase-like_central IPR028281, Sirohaem_synthase_central IPR019478, Sirohaem_synthase_dimer_dom IPR006367, Sirohaem_synthase_N IPR003043, Uropor_MeTrfase_CS |
Pfami | View protein in Pfam PF10414, CysG_dimeriser, 1 hit PF14824, Sirohm_synth_M, 1 hit PF00590, TP_methylase, 1 hit |
PIRSFi | PIRSF036426, Sirohaem_synth, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit SSF53790, SSF53790, 1 hit |
TIGRFAMsi | TIGR01469, cobA_cysG_Cterm, 1 hit TIGR01470, cysG_Nterm, 1 hit |
PROSITEi | View protein in PROSITE PS00839, SUMT_1, 1 hit PS00840, SUMT_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYSG_ECOLI | |
Accessioni | P0AEA8Primary (citable) accession number: P0AEA8 Secondary accession number(s): P11098, P76685, Q2M734 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 6, 2005 |
Last sequence update: | December 6, 2005 | |
Last modified: | December 2, 2020 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families